Evaluation of Antimicrobial Resistance Patterns of Pseudomonas aeruginosa Strains Isolated among COVID-19 Patients in Brazil Typed by Fourier-Transform Infrared Spectroscopy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains and Culture Conditions
2.2. Identification by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry Proteomic Characterization
2.3. 16S rRNA Gene Sequencing Analysis
2.4. Typing by FTIR Spectroscopy Analysis
2.5. Antimicrobial Susceptibility Profile
2.6. Biofilm Formation and Its Tolerance to Disinfectants
3. Results
3.1. Identification and Typing
3.2. Evaluation of Antimicrobial Susceptibility Profile
3.3. Evaluation of Biofilm Formation and Its Tolerance to Disinfectants
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Strain ID | Source | Patient ID | Local | Origin | Date | MALDI-TOF MS a | |
---|---|---|---|---|---|---|---|
VITEK MS® (%) b | MALDI Biotyper® (Score) c | ||||||
PS001.21 | Urine | A | ICU d-1 | COVID-19 e | 07/12/2021 | P. aeruginosa (99.9) | P. aeruginosa (2.40) |
PS002.21 | Urine | B | ICU-1 | COVID-19 | 07/14/2021 | P. aeruginosa (99.9) | P. aeruginosa (2.05) |
PS003.21 | Tracheal secretion | C | ICU-1 | COVID-19 | 07/23/2021 | P. aeruginosa (84.0) | P. aeruginosa (2.27) |
PS005.21 | Tracheal secretion | D | ICU-1 | COVID-19 | 07/30/2021 | P. aeruginosa (99.9) | P. aeruginosa (2.03) |
PS006.21 | Tracheal secretion | E | ICU-1 | COVID-19 | 07/28/2021 | P. aeruginosa (99.9) | P. aeruginosa (2.31) |
PS007.21 | Urine | F | ICU-1 | COVID-19 | 08/06/2021 | P. aeruginosa (99.9) | P. aeruginosa (2.04) |
PS008.21 | Oral swab | G | ICU-1 | COVID-19 | 08/06/2021 | P. aeruginosa (95.1) | P. aeruginosa (2.03) |
PS010.22 | Rectal swab | H | ICU-2 | HIPCC f | 01/27/2022 | P. aeruginosa (99.9) | P. aeruginosa (2.33) |
PS011.22 | Tracheal secretion | I | Medical Clinic g-1 | Non-COVID-19 | 02/17/2022 | P. aeruginosa (92.7) | P. aeruginosa (2.00) |
PS012.22 | Rectal swab | J | ICU-2 | HIPCC | 02/23/2022 | P. aeruginosa (99.9) | P. aeruginosa (2.31) |
PS013.22 | Urine | K | ICU-2 | Non-COVID-19 | 02/28/2022 | P. aeruginosa (96.1) | P. aeruginosa (2.22) |
PS014.22 | Urine | L | ICU-2 | COVID-19 | 03/01/2022 | P. aeruginosa (82.7) | P. aeruginosa (2.34) |
PS015.22 | Tracheal secretion | M | ICU-1 | COVID-19 | 02/24/2022 | P. aeruginosa (91.6) | P. aeruginosa (2.00) |
PS016.22 | Tracheal secretion | N | ICU-2 | Non-COVID-19 | 03/05/2022 | P. aeruginosa (92.0) | P. aeruginosa (2.04) |
PS017.22 | Tracheal secretion | O | ICU-1 | COVID-19 | 03/04/2022 | P. aeruginosa (99.4) | P. aeruginosa (2.26) |
PS018.22 | Tracheal secretion | C | ICU-1 | COVID-19 | 03/09/2022 | P. aeruginosa (99.9) | P. aeruginosa (2.10) |
PS019.22 | Tracheal secretion | M | ICU-1 | COVID-19 | 03/13/2022 | P. aeruginosa (99.9) | P. aeruginosa (2.01) |
PS020.22 | Urine | P | Medical Clinic-2 | Non-COVID-19 | 03/17/2022 | P. aeruginosa (99.9) | P. aeruginosa (2.07) |
Strain ID | NCBI a Access Number | Base Pair Length | Identification (%) |
---|---|---|---|
PS001/21 | OQ944132 | 1027 | P. aeruginosa (100)/P. paraeruginosa (100) |
PS002/21 | OQ944133 | 856 | P. aeruginosa (100)/P. paraeruginosa (100) |
PS003/21 | OQ944134 | 858 | P. aeruginosa (99.30)/P. paraeruginosa (99.30) |
PS005/21 | OQ945448 | 873 | P. aeruginosa (100)/P. paraeruginosa (100) |
PS006/21 | OQ945449 | 928 | P. aeruginosa (99.78)/P. paraeruginosa (99.78) |
PS007/21 | OR041497 | 1337 | P. aeruginosa (100)/P. paraeruginosa (100) |
PS008/21 | OQ945450 | 849 | P. aeruginosa (100)/P. paraeruginosa (100) |
PS010/22 | OQ945451 | 874 | P. aeruginosa (100)/P. paraeruginosa (100) |
PS011/22 | OR041498 | 1333 | P. aeruginosa (100)/P. paraeruginosa (100) |
PS012/22 | OQ945452 | 873 | P. aeruginosa (100)/P. paraeruginosa (100) |
PS013/22 | OQ945453 | 588 | P. aeruginosa (100)/P. paraeruginosa (100) |
PS014/22 | OQ945454 | 1016 | P. aeruginosa (100)/P. paraeruginosa (100) |
PS015/22 | OQ945455 | 975 | P. aeruginosa (100)/P. paraeruginosa (100) |
PS016/22 | OQ945456 | 922 | P. aeruginosa (99.89)/P. paraeruginosa (99.89) |
PS017/22 | OQ945457 | 1003 | P. aeruginosa (99.90)/P. paraeruginosa (99.90) |
PS018/22 | OQ945458 | 935 | P. aeruginosa (100)/P. paraeruginosa (100) |
PS019/22 | OQ945459 | 905 | P. aeruginosa (100)/P. paraeruginosa (100) |
PS020/22 | OQ945460 | 1025 | P. aeruginosa (99.41)/P. paraeruginosa (99.41) |
Strains | SXT | CAZ | CEF | CRO | CXM | FOX | TZP | AMI | GEN | CIP | NOR | MEM | IPM | Magiorakos et al. (2012) Classification [17] |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
PS001.21 | R | I | I | R | R | R | I | S | S | S | S | S | I | MDR |
PS002.21 | R | I | I | R | R | R | I | S | S | I | S | S | R | MDR |
PS003.21 | S | R | R | R | R | R | R | S | R | R | R | R | R | MDR |
PS005.21 | S | I | I | R | R | R | I | S | S | S | S | S | I | N-MDR |
PS006.21 | S | R | R | R | R | R | R | S | I | I | S | R | R | MDR |
PS007.21 | R | I | I | R | R | R | I | S | S | I | S | S | I | MDR |
PS008.21 | S | S | S | R | R | R | S | S | S | S | S | S | S | N-MDR |
PS010.22 | S | R | I | R | R | R | S | S | S | R | S | R | R | MDR |
PS011.22 | S | R | R | R | R | R | R | S | S | S | S | S | S | MDR |
PS012.22 | S | I | I | R | R | R | R | S | S | R | S | I | I | MDR |
PS013.22 | R | I | I | R | R | R | I | S | S | I | S | S | I | MDR |
PS014.22 | R | I | I | R | R | R | I | S | S | I | S | S | I | MDR |
PS015.22 | S | R | I | R | R | R | R | S | S | S | S | S | S | MDR |
PS016.22 | S | S | S | R | R | R | S | S | S | S | S | S | S | N-MDR |
PS017.22 | S | S | S | R | R | R | S | S | S | S | S | S | S | N-MDR |
PS018.22 | S | R | I | R | R | R | I | S | S | S | S | I | R | MDR |
PS019.22 | S | R | I | R | R | R | S | S | S | S | S | R | R | MDR |
PS020.22 | R | I | I | R | R | R | I | S | S | I | S | S | I | MDR |
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de Souza, P.A.; Santos, M.C.S.d.; de Miranda, R.V.d.S.L.; da Costa, L.V.; da Silva, R.P.P.; de Miranda, C.A.C.; da Silva, A.P.R.; Forsythe, S.J.; Bôas, M.H.S.V.; Brandão, M.L.L. Evaluation of Antimicrobial Resistance Patterns of Pseudomonas aeruginosa Strains Isolated among COVID-19 Patients in Brazil Typed by Fourier-Transform Infrared Spectroscopy. Life 2024, 14, 1079. https://doi.org/10.3390/life14091079
de Souza PA, Santos MCSd, de Miranda RVdSL, da Costa LV, da Silva RPP, de Miranda CAC, da Silva APR, Forsythe SJ, Bôas MHSV, Brandão MLL. Evaluation of Antimicrobial Resistance Patterns of Pseudomonas aeruginosa Strains Isolated among COVID-19 Patients in Brazil Typed by Fourier-Transform Infrared Spectroscopy. Life. 2024; 14(9):1079. https://doi.org/10.3390/life14091079
Chicago/Turabian Stylede Souza, Paula Araujo, Milena Cristina Silva dos Santos, Rebeca Vitória da Silva Lage de Miranda, Luciana Veloso da Costa, Raphael Paiva Paschoal da Silva, Catia Aparecida Chaia de Miranda, Ana Paula Roque da Silva, Stephen James Forsythe, Maria Helena Simões Villas Bôas, and Marcelo Luiz Lima Brandão. 2024. "Evaluation of Antimicrobial Resistance Patterns of Pseudomonas aeruginosa Strains Isolated among COVID-19 Patients in Brazil Typed by Fourier-Transform Infrared Spectroscopy" Life 14, no. 9: 1079. https://doi.org/10.3390/life14091079