Comparative Tissue Identification and Characterization of Long Non-Coding RNAs in the Globally Distributed Blue Shark Prionace glauca
Abstract
:1. Introduction
2. Materials and Methods
2.1. Tissue Sampling and RNA Isolation
2.2. Sequencing and lncRNA Identification
2.3. Tissue-Specific lncRNAs
2.4. LncRNA Differential Expression and Coding Target Genes
2.5. RT-qPCR Validation
3. Results
3.1. De Novo Assembly Overview
3.2. Identification and Characterization of lncRNAs in Blue Sharks
3.3. Identification of lncRNAs among Tissues
3.4. Tissue-Specific lncRNA Expression
3.5. Experimental RT-qPCR Validation of lncRNAs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Bravo, S.; Zarate, P.; Cari, I.; Clavijo, L.; Lopez, I.; Phillips, N.M.; Vidal, R. Comparative Tissue Identification and Characterization of Long Non-Coding RNAs in the Globally Distributed Blue Shark Prionace glauca. Life 2024, 14, 1144. https://doi.org/10.3390/life14091144
Bravo S, Zarate P, Cari I, Clavijo L, Lopez I, Phillips NM, Vidal R. Comparative Tissue Identification and Characterization of Long Non-Coding RNAs in the Globally Distributed Blue Shark Prionace glauca. Life. 2024; 14(9):1144. https://doi.org/10.3390/life14091144
Chicago/Turabian StyleBravo, Scarleth, Patricia Zarate, Ilia Cari, Ljubitza Clavijo, Ignacio Lopez, Nicole M. Phillips, and Rodrigo Vidal. 2024. "Comparative Tissue Identification and Characterization of Long Non-Coding RNAs in the Globally Distributed Blue Shark Prionace glauca" Life 14, no. 9: 1144. https://doi.org/10.3390/life14091144