Utility of Scanning Electron Microscopy Elemental Analysis Using the ‘NanoSuit’ Correlative Light and Electron Microscopy Method in the Diagnosis of Lanthanum Phosphate Deposition in the Esophagogastroduodenal Mucosa
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patient Collection
2.2. NanoSuit-CLEM Method Combined with SEM-EDS
2.3. Statistical Analysis
3. Results
3.1. NanoSuit-CLEM Method Combined with SEM-EDS Is Useful for the Diagnosis of Lanthanum Phosphate Deposition in the H&E-Stained Glass Slides
3.2. Detection of Lanthanum Phosphate Deposition in the Esophagus
3.3. Restoring of the Slides Used for the NanoSuit-CLEM Method Combined with EDS
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Case No. | Sex | Age (Years) 1 | Deposited Organ 2 | Dialysis Vintage (Years) | Length of Lanthanum Carbonate Treatment (Years, Months) | Other Pathological Findings |
---|---|---|---|---|---|---|
1 | Female | 68 | St | 20 | 5 y, 7 m | - |
2 | Male | 60 | St, Du | 32 | 3 y, 4 m | Gastric intestinal metaplasia |
3 | Male | 68 | St, Du | 4 | 2 y, 6 m | - |
4 | Male | 77 | Du | 7 | 2 y, 9 m | - |
5 | Male | 51 | Du | 7 | 1 y, 1 m | - |
6 | Male | 76 | St, Es | 21 | 5 y, 1 m | Gastric adenocarcinoma |
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Shinmura, K.; Kawasaki, H.; Baba, S.; Ohta, I.; Kato, H.; Yasuda, H.; Yamada, S.; Misawa, K.; Sugimoto, K.; Osawa, S.; et al. Utility of Scanning Electron Microscopy Elemental Analysis Using the ‘NanoSuit’ Correlative Light and Electron Microscopy Method in the Diagnosis of Lanthanum Phosphate Deposition in the Esophagogastroduodenal Mucosa. Diagnostics 2020, 10, 1. https://doi.org/10.3390/diagnostics10010001
Shinmura K, Kawasaki H, Baba S, Ohta I, Kato H, Yasuda H, Yamada S, Misawa K, Sugimoto K, Osawa S, et al. Utility of Scanning Electron Microscopy Elemental Analysis Using the ‘NanoSuit’ Correlative Light and Electron Microscopy Method in the Diagnosis of Lanthanum Phosphate Deposition in the Esophagogastroduodenal Mucosa. Diagnostics. 2020; 10(1):1. https://doi.org/10.3390/diagnostics10010001
Chicago/Turabian StyleShinmura, Kazuya, Hideya Kawasaki, Satoshi Baba, Isao Ohta, Hisami Kato, Hideo Yasuda, Satoshi Yamada, Kiyoshi Misawa, Ken Sugimoto, Satoshi Osawa, and et al. 2020. "Utility of Scanning Electron Microscopy Elemental Analysis Using the ‘NanoSuit’ Correlative Light and Electron Microscopy Method in the Diagnosis of Lanthanum Phosphate Deposition in the Esophagogastroduodenal Mucosa" Diagnostics 10, no. 1: 1. https://doi.org/10.3390/diagnostics10010001