Chromatographic Detection of 8-Hydroxy-2′-Deoxyguanosine in Leukocytes of Asbestos Exposed Workers for Assessing Past and Recent Carcinogen Exposures
Abstract
:1. Introduction
2. Material and Methods
2.1. Study Population
2.2. DNA Extraction and Purification
2.3. 32P-DNA Postlabeling
2.4. Statistical Analysis
3. Results
3.1. Demographic Variables
3.2. 8-OxodG and Asbestos Workers
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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8-OxodG per 105 Deoxyguanosine (dG) | ||||
---|---|---|---|---|
N a | Mean ± Standard Error (SE) | MR, 95% C.I. | p-Values b | |
Age (years) | ||||
≤52 | 67 | 2.3 ± 0.2 | Reference | |
53–58 | 63 | 2.1 ± 0.2 | 1.00 (0.77–1.29) | 0.978 |
≥59 | 55 | 2.1 ± 0.2 | 0.89 (0.68–1.17) | 0.408 |
Educational level | ||||
Primary education | 86 | 2.3 ± 0.2 | Reference | |
Secondary education | 72 | 2.3 ± 0.2 | 0.84 (0.67–1.06) | 0.139 |
University | 27 | 1.3 ± 0.2 | 0.65 (0.48–0.88) | 0.006 |
Smoking status | ||||
Non-smokers | 72 | 2.2 ± 0.2 | Reference | |
Ex-smokers | 66 | 2.0 ± 0.2 | 0.98 (0.67–1.43) | 0.918 |
Smokers | 46 | 2.4 ± 0.3 | 1.09 (0.74–1.60) | 0.668 |
8-OxodG per 105 Deoxyguanosine (dG) | ||||
---|---|---|---|---|
N a | Mean ± Standard Error (SE) | MR, 95% C.I. | p-Values b | |
Asbestos exposure | ||||
Unexposed controls | 87 | 1.3 ± 0.1 | Reference | |
Asbestos workers | 98 | 3.0 ± 0.2 | 2.36 (1.99–2.81) | <0.001 |
Current and past exposure | ||||
Unexposed controls | 87 | 1.3 ± 0.1 | Reference | |
Former asbestos workers | 59 | 2.9 ± 0.2 | 2.32 (1.88–2.86) | <0.001 |
Current asbestos workers | 39 | 3.1 ± 0.3 | 2.43 (1.90–3.12) | <0.001 |
≤4 years asbestos workers | 18 | 2.8 ± 0.4 | 2.21 (1.64–2.99) | <0.001 |
5–9 years asbestos workers | 21 | 2.9 ± 0.3 | 2.40 (1.79–3.23) | <0.001 |
≥10 years asbestos workers | 16 | 3.5 ± 0.5 | 2.94 (2.11–4.11) | <0.001 |
Occupational history | ||||
≤4 years | 18 | 2.8 ± 0.4 | Reference | |
5–9 years | 21 | 2.9 ± 0.3 | 1.09 (0.73–1.64) | 0.672 |
≥10 years | 16 | 3.5 ± 0.5 | 1.34 (0.85–2.10) | 0.197 |
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Cellai, F.; Bonassi, S.; Cristaudo, A.; Bonotti, A.; Neri, M.; Ceppi, M.; Bruzzone, M.; Milić, M.; Munnia, A.; Peluso, M. Chromatographic Detection of 8-Hydroxy-2′-Deoxyguanosine in Leukocytes of Asbestos Exposed Workers for Assessing Past and Recent Carcinogen Exposures. Diagnostics 2020, 10, 239. https://doi.org/10.3390/diagnostics10040239
Cellai F, Bonassi S, Cristaudo A, Bonotti A, Neri M, Ceppi M, Bruzzone M, Milić M, Munnia A, Peluso M. Chromatographic Detection of 8-Hydroxy-2′-Deoxyguanosine in Leukocytes of Asbestos Exposed Workers for Assessing Past and Recent Carcinogen Exposures. Diagnostics. 2020; 10(4):239. https://doi.org/10.3390/diagnostics10040239
Chicago/Turabian StyleCellai, Filippo, Stefano Bonassi, Alfonso Cristaudo, Alessandra Bonotti, Monica Neri, Marcello Ceppi, Marco Bruzzone, Mirta Milić, Armelle Munnia, and Marco Peluso. 2020. "Chromatographic Detection of 8-Hydroxy-2′-Deoxyguanosine in Leukocytes of Asbestos Exposed Workers for Assessing Past and Recent Carcinogen Exposures" Diagnostics 10, no. 4: 239. https://doi.org/10.3390/diagnostics10040239