Hyperoxic BOLD-MRI-Based Characterization of Breast Cancer Molecular Subtypes Is Independent of the Supplied Amount of Oxygen: A Preclinical Study

Bartsch, Silvester J.; Ehret, Viktoria; Friske, Joachim; Fröhlich, Vanessa; Laimer-Gruber, Daniela; Helbich, Thomas H.; Pinker, Katja

doi:10.3390/diagnostics13182946

Open AccessArticle

Hyperoxic BOLD-MRI-Based Characterization of Breast Cancer Molecular Subtypes Is Independent of the Supplied Amount of Oxygen: A Preclinical Study

by

Silvester J. Bartsch

^1,†,

Viktoria Ehret

^2,†

,

Joachim Friske

¹

,

Vanessa Fröhlich

³,

Daniela Laimer-Gruber

¹,

Thomas H. Helbich

¹

and

Katja Pinker

^1,4,*

¹

Department of Biomedical Imaging and Image-Guided Therapy, Division of Structural and Molecular Preclinical Imaging, Medical University of Vienna, 1090 Vienna, Austria

²

Department of Internal Medicine III, Division of Endocrinology and Metabolism, Medical University of Vienna, 1090 Vienna, Austria

³

Fachhochschule Wiener Neustadt GmbH, University of Applied Sciences, 2700 Wiener Neustadt, Austria

⁴

Breast Imaging Service, Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA

^*

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Diagnostics 2023, 13(18), 2946; https://doi.org/10.3390/diagnostics13182946

Submission received: 4 July 2023 / Revised: 12 September 2023 / Accepted: 12 September 2023 / Published: 14 September 2023

(This article belongs to the Special Issue New Horizons in Breast Cancer Magnetic Resonance Imaging)

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Abstract

Hyperoxic BOLD-MRI targeting tumor hypoxia may provide imaging biomarkers that represent breast cancer molecular subtypes without the use of injected contrast agents. However, the diagnostic performance of hyperoxic BOLD-MRI using different levels of oxygen remains unclear. We hypothesized that molecular subtype characterization with hyperoxic BOLD-MRI is feasible independently of the amount of oxygen. Twenty-three nude mice that were inoculated into the flank with luminal A (n = 9), Her2⁺ (n = 5), and triple-negative (n = 9) human breast cancer cells were imaged using a 9.4 T Bruker BioSpin system. During BOLD-MRI, anesthesia was supplemented with four different levels of oxygen (normoxic: 21%; hyperoxic: 41%, 71%, 100%). The change in the spin–spin relaxation rate in relation to the normoxic state,

Δ R_{2}^{*}

, dependent on the amount of erythrocyte-bound oxygen, was calculated using in-house MATLAB code.

Δ R_{2}^{*}

was significantly different between luminal A and Her2⁺ as well as between luminal A and triple-negative breast cancer, reflective of the less aggressive luminal A breast cancer’s ability to better deliver oxygen-rich hemoglobin to its tissue. Differences in

Δ R_{2}^{*}

between subtypes were independent of the amount of oxygen, with robust distinction already achieved with 41% oxygen. In conclusion, hyperoxic BOLD-MRI may be used as a biomarker for luminal A breast cancer identification without the use of exogenous contrast agents.

Keywords: breast cancer characterization; hypoxia; hyperoxic BOLD-MRI; intrinsic contrast MRI

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MDPI and ACS Style

Bartsch, S.J.; Ehret, V.; Friske, J.; Fröhlich, V.; Laimer-Gruber, D.; Helbich, T.H.; Pinker, K. Hyperoxic BOLD-MRI-Based Characterization of Breast Cancer Molecular Subtypes Is Independent of the Supplied Amount of Oxygen: A Preclinical Study. Diagnostics 2023, 13, 2946. https://doi.org/10.3390/diagnostics13182946

AMA Style

Bartsch SJ, Ehret V, Friske J, Fröhlich V, Laimer-Gruber D, Helbich TH, Pinker K. Hyperoxic BOLD-MRI-Based Characterization of Breast Cancer Molecular Subtypes Is Independent of the Supplied Amount of Oxygen: A Preclinical Study. Diagnostics. 2023; 13(18):2946. https://doi.org/10.3390/diagnostics13182946

Chicago/Turabian Style

Bartsch, Silvester J., Viktoria Ehret, Joachim Friske, Vanessa Fröhlich, Daniela Laimer-Gruber, Thomas H. Helbich, and Katja Pinker. 2023. "Hyperoxic BOLD-MRI-Based Characterization of Breast Cancer Molecular Subtypes Is Independent of the Supplied Amount of Oxygen: A Preclinical Study" Diagnostics 13, no. 18: 2946. https://doi.org/10.3390/diagnostics13182946

APA Style

Bartsch, S. J., Ehret, V., Friske, J., Fröhlich, V., Laimer-Gruber, D., Helbich, T. H., & Pinker, K. (2023). Hyperoxic BOLD-MRI-Based Characterization of Breast Cancer Molecular Subtypes Is Independent of the Supplied Amount of Oxygen: A Preclinical Study. Diagnostics, 13(18), 2946. https://doi.org/10.3390/diagnostics13182946

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Hyperoxic BOLD-MRI-Based Characterization of Breast Cancer Molecular Subtypes Is Independent of the Supplied Amount of Oxygen: A Preclinical Study

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