Identification of a New and Effective Marker Combination for a Standardized and Automated Bin-Based Basophil Activation Test (BAT) Analysis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Approval, Data Sets and Study Populations
2.2. Flow Cytometric Instruments and Antibodies
2.3. Basophil Activation Test
2.4. Manual Flow Cytometry Data Analysis
2.5. Development of an Automated BAT Analysis Tool Using the PRI Approach
- Pregating:
- Basophil identification:
- User-friendly result output:
2.6. Quantification and Statistical Analysis
3. Results
3.1. Re-Analysis of Mass Cytometry Data Sets Discovered a Useful New Marker Combination for BAT
3.1.1. PRI-Based Identification of the Best Basophile Identification Marker Combination
3.1.2. Characterization of CD32 Expression in Basophils
3.2. Own Flow Cytometry Data Validate Re-Analyzed Mass Cytometry Results
3.2.1. New Marker Combination Confirms Its Potential in Bin-Based BAT Analysis
3.2.2. Flow Cytometry Data Confirms Correlation of CD32, FcεRIα and CD63 Expression Patterns on Basophils
3.2.3. Tendency of Higher CD32 Expression Values on Basophils from Non-Allergic Patients Compared to Allergic Patients
3.3. Successful Automation of BAT Analyses Using PRI Approach
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Groffmann, J.; Hoppe, I.; Ahmed, W.A.N.; Hoang, Y.; Gryzik, S.; Radbruch, A.; Worm, M.; Beyer, K.; Baumgrass, R. Identification of a New and Effective Marker Combination for a Standardized and Automated Bin-Based Basophil Activation Test (BAT) Analysis. Diagnostics 2024, 14, 1959. https://doi.org/10.3390/diagnostics14171959
Groffmann J, Hoppe I, Ahmed WAN, Hoang Y, Gryzik S, Radbruch A, Worm M, Beyer K, Baumgrass R. Identification of a New and Effective Marker Combination for a Standardized and Automated Bin-Based Basophil Activation Test (BAT) Analysis. Diagnostics. 2024; 14(17):1959. https://doi.org/10.3390/diagnostics14171959
Chicago/Turabian StyleGroffmann, Johannes, Ines Hoppe, Wail Abbas Nasser Ahmed, Yen Hoang, Stefanie Gryzik, Andreas Radbruch, Margitta Worm, Kirsten Beyer, and Ria Baumgrass. 2024. "Identification of a New and Effective Marker Combination for a Standardized and Automated Bin-Based Basophil Activation Test (BAT) Analysis" Diagnostics 14, no. 17: 1959. https://doi.org/10.3390/diagnostics14171959