This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Open AccessArticle
Comparative Analysis of HPV Detection Efficiency: Evaluating Cobas 8800 Performance in Vaginal Self-Sampling versus Clinician-Collected Samples at a Regional Thai Hospital
by
Umaporn Ruttanamora
Umaporn Ruttanamora 1,
Pinsawitar Thongsalak
Pinsawitar Thongsalak 2,
Araya Sammor
Araya Sammor 3,
Sirinart Chomean
Sirinart Chomean 2,4 and
Chollanot Kaset
Chollanot Kaset 2,4,*
1
Graduate Program in Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathum Thani 12120, Thailand
2
Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathum Thani 12120, Thailand
3
Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand
4
Thammasat University Research Unit in Medical Technology and Precision Medicine Innovation, Thammasat University, Pathum Thani 12120, Thailand
*
Author to whom correspondence should be addressed.
Diagnostics 2024, 14(19), 2177; https://doi.org/10.3390/diagnostics14192177 (registering DOI)
Submission received: 1 July 2024
/
Revised: 11 September 2024
/
Accepted: 25 September 2024
/
Published: 29 September 2024
Abstract
Background: This study, conducted at a regional Thai hospital, assesses the comparative efficacy of self-collected versus clinician-collected samples for HPV detection using the Cobas 8800 system among Thai women aged 30–60. Methods: Our methodology involved analyzing 1541 self-collected and 1398 clinician-collected samples. Results: The results show a statistically significant mean difference in cycle threshold (Ct) values favoring clinician-collected samples (1.53; 95% CI: 1.18–1.87, p < 0.0001). This pattern was consistent across various age groups, with the most pronounced differences noted in the oldest cohort (50–59 years), suggesting higher detection efficiency in clinician-collected samples. The study further explored the correlation of Ct values with cytological and histological outcomes, where clinician-collected samples demonstrated superior diagnostic performance, particularly in identifying LSIL and HSIL conditions, evidenced by AUC values of 0.793 and 0.866, respectively. While self-sampling remains a viable method, with sensitivity reaching up to 48.84% for LSIL and 46.15% for HSIL, clinician collection proved more accurate, likely influencing future national screening policies. Conclusions: This work underscores the need for robust sample collection methods and the importance of ongoing enhancements to self-sampling assays and techniques to ensure their efficacy in cervical cancer screening programs.
Share and Cite
MDPI and ACS Style
Ruttanamora, U.; Thongsalak, P.; Sammor, A.; Chomean, S.; Kaset, C.
Comparative Analysis of HPV Detection Efficiency: Evaluating Cobas 8800 Performance in Vaginal Self-Sampling versus Clinician-Collected Samples at a Regional Thai Hospital. Diagnostics 2024, 14, 2177.
https://doi.org/10.3390/diagnostics14192177
AMA Style
Ruttanamora U, Thongsalak P, Sammor A, Chomean S, Kaset C.
Comparative Analysis of HPV Detection Efficiency: Evaluating Cobas 8800 Performance in Vaginal Self-Sampling versus Clinician-Collected Samples at a Regional Thai Hospital. Diagnostics. 2024; 14(19):2177.
https://doi.org/10.3390/diagnostics14192177
Chicago/Turabian Style
Ruttanamora, Umaporn, Pinsawitar Thongsalak, Araya Sammor, Sirinart Chomean, and Chollanot Kaset.
2024. "Comparative Analysis of HPV Detection Efficiency: Evaluating Cobas 8800 Performance in Vaginal Self-Sampling versus Clinician-Collected Samples at a Regional Thai Hospital" Diagnostics 14, no. 19: 2177.
https://doi.org/10.3390/diagnostics14192177
Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details
here.
Article Metrics
Article Access Statistics
For more information on the journal statistics, click
here.
Multiple requests from the same IP address are counted as one view.