Detection of Rickettsiae, Borreliae, and Ehrlichiae in Ticks Collected from Walker County, Texas, 2017–2018
Abstract
:1. Introduction
2. Materials and Methods
2.1. Tick Collection, Identification and Nucleic Acid Extraction
2.2. PCR Amplification and RFLP for Tick-borne Bacterial and Rickettsial Detection
2.3. Detection of Tick-borne Viruses by Quantitative RT-PCR
2.4. Nucleotide Sequencing and Analysis
3. Results
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Collection Date | Species | Life Stage/Sex | Total Number |
---|---|---|---|
29 June 2017 | A. americanum | nymph | 190 |
female | 115 | ||
male | 116 | ||
A. maculatum | male | 1 | |
D. variabilis | female | 6 | |
male | 10 | ||
5 October 2017 | A. americanum | nymph | 150 |
female | 1 | ||
male | 2 | ||
I. scapularis | female | 2 | |
male | 1 | ||
5 June 2018 | A. americanum | nymph | 175 |
female | 93 | ||
male | 92 | ||
A. maculatum | male | 1 | |
D. variabilis | female | 3 | |
male | 6 | ||
I. scapularis | nymph | 1 | |
male | 1 |
Tick Species | Total Pools | Pools with Positive Bacterial Sequences | ||||
---|---|---|---|---|---|---|
Borrelia | Ehrlichia | Rickettsia | ||||
lonestari | chaffeensis | ewingii | amblyommatis | endosymbiont | ||
Amblyomma americanum | 68 | 3 | 6 | 11 | 68 | 0 |
A. maculatum | 2 | 0 | 0 | 0 | 1 | 0 |
Dermacentor variabilis | 6 | 0 | 0 | 0 | 0 | 0 |
Ixodes scapularis | 4 | 0 | 0 | 0 | 0 | 2 |
Total | 80 | 3 | 6 | 11 | 69 | 2 |
Collection Date | Borrelia lonestari | Ehrlichia chaffeensis | Ehrlichia ewingii | Rickettsia amblyommatis | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
AF | AM | N | AF | AM | N | AF | AM | N | AF | AM | N | |
29 June 2017 | 1/12 | 1/12 | 0/9 | 1/12 | 2/12 | 1/9 | 1/12 | 2/12 | 1/9 | 12/12 | 12/12 | 9/9 |
5 October 2017 | 0/1 | 0/1 | 1/6 | 0/1 | 0/1 | 1/6 | 0/1 | 0/1 | 2/6 | 1/1 | 1/1 | 6/6 |
5 June 2018 | 0/10 | 0/10 | 0/7 | 1/10 | 0/10 | 0/7 | 3/10 | 2/10 | 0/7 | 10/10 | 10/10 | 7/7 |
Total | 1/23 | 1/23 | 1/22 | 2/23 | 2/23 | 2/22 | 4/23 | 4/23 | 3/22 | 23/23 | 23/23 | 22/22 |
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Mendell, N.L.; Reynolds, E.S.; Blanton, L.S.; Hermance, M.E.; Londoño, A.F.; Hart, C.E.; Quade, B.R.; Esterly, A.T.; Hendrix, C.B.; Teel, P.D.; et al. Detection of Rickettsiae, Borreliae, and Ehrlichiae in Ticks Collected from Walker County, Texas, 2017–2018. Insects 2019, 10, 315. https://doi.org/10.3390/insects10100315
Mendell NL, Reynolds ES, Blanton LS, Hermance ME, Londoño AF, Hart CE, Quade BR, Esterly AT, Hendrix CB, Teel PD, et al. Detection of Rickettsiae, Borreliae, and Ehrlichiae in Ticks Collected from Walker County, Texas, 2017–2018. Insects. 2019; 10(10):315. https://doi.org/10.3390/insects10100315
Chicago/Turabian StyleMendell, Nicole L., Erin S. Reynolds, Lucas S. Blanton, Meghan E. Hermance, Andres F. Londoño, Charles E. Hart, Bethany R. Quade, Allen T. Esterly, C’Brionne B. Hendrix, Pete D. Teel, and et al. 2019. "Detection of Rickettsiae, Borreliae, and Ehrlichiae in Ticks Collected from Walker County, Texas, 2017–2018" Insects 10, no. 10: 315. https://doi.org/10.3390/insects10100315