Similar Gut Bacterial Microbiota in Two Fruit-Feeding Moth Pests Collected from Different Host Species and Locations
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sample Collection and DNA Extraction
2.2. 16S rRNA Gene Amplification and Sequencing
2.3. Quality Control and OTU Identification
2.4. Diversity Analysis
3. Results
3.1. Community Composition of the Gut Microbiota in PFM and OFM
3.2. Comparison on Gut Microbiota between PFM and OFM
3.3. Influence of Orchard on Gut Microbiota within Species
4. Discussion
4.1. Comparison of Gut Microbiota from Two Fruit Borers
4.2. Influence of Orchard and Host Species on Gut Microbiota
4.3. Wolbachia in PFM and OFM
4.4. Implications for Pest Management
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Code * | Species | Collecting Location | Host (code) | Coordinate | NO. |
---|---|---|---|---|---|
PKPR | PFM | Kaosanji of Pinggu district (K) | Pear (PR) | 40°12′ N, 117°19′ E | 5 |
OKPR | OFM | 5 | |||
PLPR | PFM | Lvfulong of Yanqing district (L) | Pear (PR) | 40°32′ N, 116°4′ E | 5 |
OLPR | OFM | 7 | |||
PGAE | PFM | Liugou of Yanqing district (G) | Apple (AE) | 40°27′ N, 116°6′ E | 4 |
OGAE | OFM | 6 | |||
PDAE | PFM | Dafengying of Yanqing district (D) | Apple (AE) | 40°26′ N, 115°54′ E | 5 |
OYPH | OFM | Linguosuo of Haidian district (Y) | Peach shoot (PH) | 39°58′ N, 116°13′ E | 5 |
OSPH | OFM | Shuangxin of Haidian district (S) | Peach shoot (PH) | 39°57′ N, 116°12′ E | 2 |
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Gong, Q.; Cao, L.-J.; Sun, L.-N.; Chen, J.-C.; Gong, Y.-J.; Pu, D.-Q.; Huang, Q.; Hoffmann, A.A.; Wei, S.-J. Similar Gut Bacterial Microbiota in Two Fruit-Feeding Moth Pests Collected from Different Host Species and Locations. Insects 2020, 11, 840. https://doi.org/10.3390/insects11120840
Gong Q, Cao L-J, Sun L-N, Chen J-C, Gong Y-J, Pu D-Q, Huang Q, Hoffmann AA, Wei S-J. Similar Gut Bacterial Microbiota in Two Fruit-Feeding Moth Pests Collected from Different Host Species and Locations. Insects. 2020; 11(12):840. https://doi.org/10.3390/insects11120840
Chicago/Turabian StyleGong, Qiang, Li-Jun Cao, Li-Na Sun, Jin-Cui Chen, Ya-Jun Gong, De-Qiang Pu, Qiong Huang, Ary Anthony Hoffmann, and Shu-Jun Wei. 2020. "Similar Gut Bacterial Microbiota in Two Fruit-Feeding Moth Pests Collected from Different Host Species and Locations" Insects 11, no. 12: 840. https://doi.org/10.3390/insects11120840