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Article

Landscape Simplification Modifies Trap-Nesting Bee and Wasp Communities in the Subtropics

by
Rachele S. Wilson
1,2,*,
Sara D. Leonhardt
3,
Chris J. Burwell
2,4,5,
Chris Fuller
6,
Tobias J. Smith
7,
Benjamin F. Kaluza
8 and
Helen M. Wallace
2
1
Genecology Research Centre, University of the Sunshine Coast, 90 Sippy Downs Dr, Sippy Downs, QLD 4556, Australia
2
Environmental Futures Research Institute, Griffith University, 170 Kessels Rd, Nathan, QLD 4111, Australia
3
Department of Ecology and Ecosystem Management, Technical University of Munich, Hans-Carl-von-Carlowitz-Platz 2, 85354 Freising, Germany
4
Biodiversity Program, Queensland Museum, PO Box 3300, South Brisbane, QLD 4101, Australia
5
School of Environment and Science, Griffith University, Nathan, QLD 4111, Australia
6
Kin Kin Native Bees, Main St, Kin Kin, QLD 4571, Australia
7
School of Biological Sciences, University of Queensland, St Lucia, QLD 4072, Australia
8
Department of Public Technology and Innovation Planning, Fraunhofer Institute for Technological Trend Analysis INT, Appelsgarten 2, 53879 Euskirchen, Germany
*
Author to whom correspondence should be addressed.
Insects 2020, 11(12), 853; https://doi.org/10.3390/insects11120853
Submission received: 5 November 2020 / Revised: 26 November 2020 / Accepted: 27 November 2020 / Published: 1 December 2020
(This article belongs to the Section Insect Ecology, Diversity and Conservation)
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Abstract

:

Simple Summary

Many bees and wasps are important pollinators and natural pest controllers. Habitat loss is a major threat to bee and wasp conservation, but little is known about how this impacts tropical bees and wasps. This study aimed to determine how habitat loss affects solitary bees and wasps in tropical agricultural landscapes and how they change with the seasons. Solitary bees and wasps can be monitored using trap nests, popularly known as “bee hotels”. We installed bee hotels in forests and orchards and checked them every season over two years. We found 41 species of bees and wasps nesting in bee hotels. Importantly, five species of bees and 14 species of wasps were found only in forests, mostly species with particular food or nesting requirements. More species of bees and wasps used the hotels in the wet season (spring-summer). Our study suggests that solitary bees and wasps with special resource requirements are vulnerable to habitat loss in tropical agricultural landscapes.

Abstract

(1) Background: Landscape simplification is a major threat to bee and wasp conservation in the tropics, but reliable, long-term population data are lacking. We investigated how community composition, diversity, and abundance of tropical solitary bees and wasps change with landscape simplification (plant diversity, plant richness, distance from forest, forest cover, and land use type) and season. (2) Methods: We installed 336 timber and cob trap nests in four complex forests and three simplified orchards within the subtropical biodiversity hotspot of south-east Queensland, Australia. Trap nests were replaced every season for 23 months and all emergents identified. (3) Results: We identified 28 wasp species and 13 bee species from 2251 brood cells. Bee and wasp community composition changed with landscape simplification such that large, ground-nesting, and spider-hunting species were present in all landscapes, while those with specialist resource requirements and (clepto) parasitoids were present only in complex landscapes. Abundance and diversity of bees and wasps were unaffected by landscape simplification but increased with rainfall. (4) Conclusions: This study highlights the need for multi-year studies incorporating nuanced measures such as composition with a focus on functional diversity to detect changes bee and wasp populations.

1. Introduction

Simplified landscapes are landscapes with little variation in land cover types, vertical vegetation structure, and plant diversity [1,2]. Agricultural expansion is the largest contributor to landscape simplification worldwide [3,4]. Simplified landscapes provide less resource diversity, niches, and species diversity than complex landscapes such as forests [2,5,6]. Landscape simplification is known to alter the abundance and diversity of many taxonomic groups, including birds [7], mammals [8], and invertebrates such as bees and wasps [9]. Bees and wasps provide essential pollination and pest management services, yet can be very sensitive to landscape simplification [10,11]. Maintaining bee and wasp communities in simplified landscapes, such as agroecosystems, therefore requires an understanding of their responses to respective changes in the landscape.
Natural or non-crop land in agriculture (e.g., forest, riparian vegetation, and fallow fields) acts as reservoirs for biodiversity and provides resources for bees and wasps [10,12,13,14]. Wild bee abundance and richness is lower in farms with little natural land use, resulting in lower flower visitation and crop yields [13,14]. Similarly, wasp diversity, abundance, and activity in fields can decrease with increasing distance to, or between patches of natural vegetation [10,12]. However, not all bee and wasp taxa are affected by landscape simplification in the same way, with traits such as nesting strategy (above- or below-ground) or sociality largely determining responses [15,16,17,18,19]. Furthermore, most research on bee and wasp responses to landscape simplification has been conducted in temperate regions of North America, Europe, and South America (see meta-analyses by [11,20,21,22]). Similar studies of bee and wasp responses in tropical and subtropical agroecosystems are rare [21], despite much of the tropics undergoing significant land use change [3].
Tropical regions have diverse bee and wasp faunas with potentially many thousands of species still waiting to be described [23,24]. Landscape simplification is considered a major threat to bee and wasp conservation in the tropics, but reliable population data is still needed to support further conclusions [23]. Nevertheless, some research suggests that different aspects of forests play an important role in tropical bee and wasp communities. For example, bee and wasp diversity and composition differs between forest and agricultural land uses in Brazil [25], Ecuador [26], and Costa Rica [15]. Within forests, bee and wasp diversity has been found to increase with canopy cover and fragment size in Australia [17,27], Colombia [28], and Mexico [29]. However, the longest tropical studies (two years), both in Brazil, show conflicting results with landscape simplification such as both increased [6] and decreased bee abundance [16]. This highlights the need for more long-term studies in tropical regions to understand the effects of landscape simplification.
Studies of bee or wasp responses to landscape simplification have largely been limited in their temporal observations, particularly in agricultural landscapes where sampling typically occurs over only one season of crop flowering (e.g., [30,31]). Observations across multiple seasons are important for showing changes in communities and thus ecosystem functions, such as from pollination by bee-dominated communities in spring to predation by wasp-dominated communities in summer [32]. The increasing use of trap nests for research presents opportunities to efficiently lengthen observations to several seasons or years (e.g., [6,33]), thus enabling more detailed, long-term investigations into solitary bee and wasp communities. Trap nests (also called “bee hotels” or “Fabre’s hives”) are artificially constructed nesting boxes for the study and management of solitary bees, wasps, and other insects (see reviews by [21,34]). Most trap-nest studies have measured responses of above-ground nesting taxa (i.e., those that nest in reeds or timber cavities), despite “below-ground nesters” dominating solitary bee species [24]. The few studies that have included below-ground nesters have used soil squares in situ [35] or portable cob structures as trap nests [36].
This study aimed to determine how landscape simplification and seasonal variability influence trap-nesting bee and wasp communities in the subtropics, using the biodiversity hotspot of south-east Queensland [37], Australia, as a case study. We asked how does community composition, diversity, and abundance of above- and below-ground nesting bees and wasps change with landscape simplification and season? As bee and wasp diversity can increase with landscape complexity in other bioregions, we hypothesise that: (1) community composition of bees and wasps will change with landscape simplification; (2) bee and wasp species diversity will be reduced in simplified landscapes; (3) bee and wasp abundance will be reduced in simplified landscapes; and (4) abundance and diversity of bees and wasps will vary with the seasons, decreasing in the cool, dry months.

2. Materials and Methods

Seven study sites were established in south-east Queensland, Australia (−24°38′ to −27°29′ S, 152°6′ to 153°6′ E). Sites consisted of natural forests dominated by an overstorey of Eucalyptus and Corymbia species (four sites) or orchards that consisted of a complex matrix of commercial macadamia plantings (Macadamia integrifolia Maiden and Betche × M. tetraphylla Johnson) and forest fragments (three sites, Figure 1). We measured landscape simplification with the following metrics: dominant land use, plant richness, percentage of land cover type (forest and orchard), and distance to forest [13,19] (Table 1). Study sites selected had ≥75% of target forest or orchard cover [38]. There was a minimum distance of 1 km between all sites, 6 km between sites of different land use types, and approximately 200 km between northern and southern blocks of sites. Plant species at study sites were described by Kaluza et al. [38,39]. Forest sites generally had twice the plant species richness of orchard sites (Table 1) and a more complex vertical structure (Figure 1).
Trap nests were installed in all sites in October 2016. Trap nests were housed in metal units, fixed to steel pickets, 1 m above the ground, with a minimum distance between each unit of 5 m. All trap nests were oriented in the same direction (north-east) to face away from the prevailing winds of the cooler months and to reduce irregular colonization of cavities [21]. Four units were installed at each site, with each unit comprising 12 possible nests (10 timber cavities and 2 cob blocks). In total, 336 trap nests (16 units × 12 trap nests across the four forest sites and 12 units × 12 trap nests across the three orchard sites) were available for occupation across all sites in the first season, followed by 264 trap nests (12 units × 12 trap nests across forest sites and 10 units × 12 trap nests across orchard sites) per season, due to the loss of six units in February 2017 to theft and damage.
Three different nesting substrates were provided in each unit to accommodate nesting preferences of “above-ground” and “below-ground” nesters [18]. Pre-drilled timber blocks were provided for species that nest above-ground in existing hollow cavities (e.g., Megachile and Hylaeus spp.). This design was chosen to allow whole nest management, as the holes can be lined with paper tubes (“PaperTunnel” liners, Pollinator Paradise, Parma, ID, USA) for easy removal of nests and reuse of blocks. Cavities of different dimensions were used to attract differently sized bee species. Five short cavities (100 mm long × 6 mm diameter) and five long cavities (150 mm long × 8 mm diameter) drilled into timber blocks were provided for each nesting unit, with the entrances slightly charred before inserting tubes. Cob blocks were provided for below-ground nesting bees that prefer sandy loam soil types such as Amegilla spp. [40]. Two cob blocks (a 4:1 mixture of sand and clay) were provided for each nesting unit, with two starter entrance holes pre-formed in each block. Each cob block represents one possible nest, however, as there was no barrier within the cob to separate potential ground-nester tunnels. Mixed bunches of Xanthorrhoea sp. and Lantana sp. stems with soft pith were also included as substrates for species that excavate their own above-ground cavities (e.g., allodapine bees). These pithy stems showed no signs of occupation, however, and so were excluded from analyses (as in [41]).
Trap nest units were checked for occupation every three to four months from February 2017 to December 2018. Occupied trap nests were removed and replaced with fresh paper tubes or cob blocks each sampling period. Occupied nests were placed in mesh (cob) or organza (paper tubes) bags for transport until dissection. All paper tubes recovered were carefully dissected to record the number of brood cells (abundance, above-ground nesters), larvae, pupae, adults, nest material, provisions, and pests or parasites. We assumed that the number of nests gives an estimation of the number of nesting females and that the number of brood cells per nest is an estimation of fecundity [42], although it is possible that females constructed multiple nests per block and additional nests in other units or locations.
Dissected nests with larvae or pupae were resealed, transferred to clean organza bags (one nest per bag), and stored together in emergence boxes outdoors [43]. All cob nests recovered were stored outdoors in larger mesh bags (one block per bag) without dissection, to avoid destroying the fragile structures that support emergence in these bees. All emergents from cob nests were counted (abundance of below-ground nesters). All nests were checked monthly for emergents, which were then frozen at −18˚C and pinned for identification. Emergent bees and wasps were identified to species or morphospecies by CJB using available taxonomic literature and reference to collections in the Queensland Museum. Nests without adult emergents (e.g., dead larvae or pupae) were determined to likely host family or subfamily from nest materials or provisions. For example, nests with pollen and “cellophane” to Hylaeinae and of resin or leaves to Megachilidae. Nests that were vacated prior to field sampling were counted as “occupied” without further species determination or abundance record [44].
We tested the effects of seasonal variation (season, temperature, and precipitation) and landscape simplification (plant richness, distance from forest, forest cover, and land use type) on the response variables of bee and wasp species composition, diversity (richness and Shannon), and abundance using Generalized Linear Mixed Models (GLMMs). Daily maximum temperature and rainfall observations were retrieved from the Australian Bureau of Meteorology (bom.gov.au/climate/data/index.shtml) and averaged for weather stations closest to the northern (Station No. 039128, 14 km south of P3) and southern (Station No. 040861, 15 km north of F3) sites over each sampling period. Season was defined as the season in which trap nests were sampled: summer (December-February), autumn (March-May), winter (June-August), and spring (September-November). Bee and wasp species diversity was calculated using Shannon’s diversity index: H = ∑ i = 1 s − ( P i ×   ln P i ) , where s is the total number of species and P i is the number of individuals of “species i” per site, divided by the total number of individuals for all species (Data S1). Bee abundance was segregated according to nesting strategy (above-ground and below-ground nesters), to account for different approaches used to measure abundance (i.e., number of brood cells vs. number of emergents) (Data S1). Bee diversity and richness was aggregated for all bees, as species identification was independent of nesting strategy (Data S1).
All analyses were performed in the statistical software ‘R’ (v 3.5.2, [45]) (Script S1). We used the vegan package to ordinate bee and wasp communities with non-metric multidimensional scaling (NMDS) using the metaMDS() function and visualised the data using Bray–Curtis distances with three dimensions and up to 999 permutations [46]. Effects of environmental variables on communities were tested individually with permutational multivariate ANOVAs using the vegdist() and adonis() functions, also with Bray-Curtis distance matrices [46,47]. Significant environmental variables were then fitted to NMDS models and visualised together with the envfit() function [46]. Similarity percentages to discriminate species between categorical groups was done using Bray-Curtis dissimilarities with the simper() function [46]. Data used for ordination and to construct GLMMs was analysed per trap nest unit. GLMMs were composed for each response variable, starting with the most complex model including all explanatory variables of interest and interactions between them, except where such variables were significantly correlated (i.e., season and precipitation; land cover, land use, and distance from forest). Where variables were significantly correlated, several models were composed each including only one of the correlated variables (e.g., model 1 = y ~ season*plant richness, model 2 = y ~ precipitation*plant richness). Random effects included in all models were trap nest unit, nested within site. An observation-level random effect was also added to GLMMs that were overdispersed [48]. Models were then simplified by consecutively dropping variables and comparing models with and without variables with likelihood ratio tests (χ2) using the anova() function until the most parsimonious model was reached. Final explanatory models were compared against null models (i.e., random factors only) to assess the significance levels of remaining variables [49]. Explanatory power of final models was determined from the variance of fixed (marginal R²) and random effects (conditional R²) with the MuMIn package [50]. Differences between levels of categorical explanatory factors were compared with Tukey’s post hoc tests using the glht() function [51].

3. Results

A total of 654 nests were constructed in trap nest cavities or cob blocks over the entire sampling period of 23 months (36% occupation of available cavities and cob). We recovered 590 of these nests for analysis. Nests that were incomplete (less than one cell) were excluded from further analyses (n = 28). Of the remaining 562 nests recovered (Table 2): 200 had at least one adult emergent from which we could identify the host species; 79 were determined to the most likely host (sub)family from nest materials and provisions; and 283 nests, all constructed with mud and/or provisioned with spiders, could not be determined beyond “wasps”.

3.1. Community Composition

Adult emergents from recovered nests were classified into 41 different species or morphospecies (Table S1). Trap nest communities were mostly comprised of wasps (28 species, 74% of nests) rather than bees (13 species, 26% of nests). Five bee species occurred only in forests and two bee species occurred only in orchards (Table 2). The remaining six bee species were recorded in both land uses (Table 2). Similarly, for wasps, seven species were observed in both land uses while 14 species were found only in forests and seven species only in orchards (Table 2).
Trap-nesting bee and wasp communities found at our sites exhibited a range of different functional traits. Most were nesting above-ground, including seven bee species (out of 13) and all wasps (Table 2). Bees were also found to use different nesting materials, including “cellophane” secretions (three hylaeine species), leaves (Megachile simplex), resin (three Megachile spp.), and the supplied cob mixture (ground-nesting Apidae and Pachyprosopis spp.) (Table 2). Non-parasitic wasp species provisioned brood cells with either spiders (13 species, Pompilidae and Crabronidae) or caterpillars (five Eumeninae species) and used mud or cob to construct nests (Table 2).
Most nests were provisioned by one host species, except for 25 nests which were either usurped or co-inhabited by two or more species. In particular, adults of the ground-nesting Amegilla adelaidae and both Pachyprosopis spp. often emerged from the same nest. Others were cavities filled with both wasp (Pison spp.) and bee (Megachile mystacaena or Hylaeus spp.) brood cells, usually in succession. Almost one-third of wasp species identified were parasitic, with most parasitising other wasp nests, except Gasteruption spp., which were found in nests of resin bees (M. mystaceana) and cellophane bees (Hylaeinae spp.). Other pests of bees included cleptoparasitic Thyreus spp., which were recorded in nests of both Amegilla species.
Communities of bees and wasps were influenced differently by landscape simplification and season (Table 3). Bee communities were influenced by all environmental factors except rainfall, with the strongest effects being season, temperature, and amount of forest cover (Table 3). Bee community composition differed with land use, but also distance to forest, forest cover, and plant richness (Figure 2A). Wasp communities were influenced by all environmental factors except plant richness (Table 3). Composition of wasp communities differed with land use, percentage of forest cover, and distance to forest (Figure 2B).
A SIMPER analysis identified three bee species, M. simplex, A. adelaidae, and M. mystacaena, that collectively accounted for over 60% of the total bee community dissimilarity between forests and orchards (Table 4). These species were the most abundant individuals in trap nests, collectively totalling 203 of the 574 brood cells recovered from bee nests (Table 2). While M. simplex increased in abundance from forests to orchards, A. adelaidae was the opposite and M. mystaceaena was found only in forests. Similarly, four wasp species contributed the most to total wasp community dissimilarity between land uses: Pison sp. B, Pison sp. E, Fabriogenia sp. B and Pison sp. D (Table 4). Of these, Fabriogenia sp. B and Pison sp. B decreased in abundance from forests to orchards, Pison sp. E was found only in forests and Pison sp. D was found only in orchards (Table 2).

3.2. Species Diversity and Abundance

Variation in the species diversity and richness of bees and wasps was mostly explained by season or random factors rather than landscape simplification (Table 5). Bee diversity varied with season, and was higher in spring compared to autumn (Table 5). Bee species richness was not explained by any of our factors of interest, with similar numbers of species occurring across seasons and landscapes.
Bee abundance was unaffected by our measures of landscape simplification. The most abundant bee families were Megachilidae (49% of bee nests) and Colletidae (26%) (Table 2). Above-ground bee abundance increased with rainfall and varied with season, such that abundance was higher in summer compared to autumn (Table 5). Below-ground bee abundance similarly increased with rainfall and varied with season, with the lowest abundance in autumn (Table 5).
Wasp diversity was similarly influenced by season, being higher in summer compared to autumn (Table 5). Wasp species richness and abundance were best explained by random factors, with similar numbers of species and individuals occurring across seasons and landscapes. The most abundant wasp families were Crabronidae (57% of identified wasp nests) and Pompilidae (17%) (Table 2).

4. Discussion

We found that the composition of bee communities changed with land use type, amount of forest cover, distance to forest, plant diversity, and richness. Similarly, wasp community composition differed with land use type, amount of forest cover, and distance to forest, but not plant diversity or richness. The latter suggests that bees and wasps are affected differently by landscape simplification and require different resources to persist in subtropical agroecosystems. Importantly, five species of bees and 14 species of wasps were found only in forests, mostly species with specialist food or nesting requirements. In contrast to our expectations, we found diversity and abundance of bees and wasps varied with the seasons or rainfall, but not landscape simplification. Our study shows that, while overall numbers and diversity of tropical solitary bees and wasps may remain stable with landscape simplification, the composition of those communities is vulnerable to change.
The observed changes in bee and wasp community composition with landscape simplification was consistent with our first hypothesis. Similar changes were found by other trap-nest studies in tropical agroecosystems [6,15,25]. For example, plant diversity or richness was also a key determinant of bee community composition in Brazil [6]. The strong effect of plant diversity is probably due to changes in the availability of plant-based food (e.g., pollen) and nesting resources. Changes in wasp community composition with different forest metrics may indicate that there are forest-dependent species in each land use, including some species that favour less forest cover or shade (e.g., those found in the orchards only). Forests are important sources of food and nesting materials for wasps [21,52], where prey items may be more abundant irrespective of plant diversity and composition (e.g., caterpillars [53] and spiders [54]).
The composition in each land use is likely dependent on the traits of bee and wasp assemblages in our study system such as body size, foraging distance, resource use, and life history strategies such as parasitism (e.g., [16,28,55,56,57,58]). For example, the three bee and four wasp species that accounted for most dissimilarity between land uses (A. adelaidae, M. mystacaena, M. simplex, Fabriogenia sp. B, Pison sp. B, Pison sp. D, and Pison sp. E) were all large species, ground-nesters or spider-hunters. Those taxa present only in complex landscapes were mostly species with (known) specialist resource requirements (e.g., M. mystacaena, which needs resin-bearing trees) and the (clepto) parasitoids, which are dependent on the abundance and richness of their hosts [56,57]. Specialisation determines the breadth of resources exploited: specialists collect from a narrow range of (often related) resources, while generalists have a broader diet [59]. Specialists should be more vulnerable to decline when faced with shortages in their hosts, however, some can broaden their resource use during times of scarcity–if similar (morphologically or chemically) resources are available [60,61]. More comparative studies of bee and wasp resource use in different environments are needed to better understand the responses of specialists and generalists to changes in resource availability [62].
Contrary to our second and third hypotheses, the diversity and abundance of bees and wasps were not reduced in simplified landscapes. Instead, we saw similar abundances and numbers of bee and wasp species across sites with different levels of landscape complexity. Most studies found wild bee diversity and abundance is reduced in simplified agricultural landscapes (see reviews by [11,63]); or that diversity is reduced, but abundance is increased (e.g., with mass-flowering crops [64]). Our results may differ from these studies because of the inherently high biodiversity in the study region, even with landscape simplification. Of all our floristically simplified study sites, those with the least plant diversity (site “P2”; Table 1) still had at least 39 to 50 flowering plant species within 500 m of trap nests in addition to the mass-flowering macadamia. This could indicate that even simplified landscapes support relatively high trap-nesting bee and wasp diversity in the tropics—particularly of generalist species—as simplified tropical landscapes can still be relatively rich in plant species and thus provide more diet options compared with simplified temperate landscapes [62,65,66]. Furthermore, the comparatively short-term nature of similar trap-nest studies in tropical agroecosystems (e.g., 6–12 months [15,25]) may not capture the true variation of bee diversity and abundance following landscape simplification. However, this phenomenon could be restricted to cavity- or trap-nesting bees and wasps, due to their ability to nest in vertical soil or man-made cavities (e.g., studies using nets [16]; but see [15]).
Our hypothesis that diversity and abundance of bees and wasps will vary with the seasons, decreasing in the cool, dry months, was upheld. We found both above- and below-ground bee abundance to be positively associated with rainfall, being lowest in the cool, dry months of autumn. Diversity of bees and wasps was similarly lowest in autumn and highest in spring for bees and summer for wasps. In the tropics, many solitary bee species can reproduce continuously throughout the year, but particularly in the warm, wet season (e.g., in Ecuador [26]). Such patterns are likely also related to changes in resource availability [32]. As such, our findings suggest that bee and wasp communities in the subtropics follow similar seasonal patterns to those in the tropics.
We identified 28 wasp species (74% of nests) and 13 bee species (26% of nests) in trap nests. This is higher than the number of cavity-nesting species found in similar agroecological studies in temperate bioregions (e.g., [10,67]) and similar to those in the neotropics (e.g., [6]). Such parallels support the use of trap nests as indicators of bee and wasp diversity in different bioregions. Seeing more wasp than bee occupation is a common finding among trap nest studies (e.g., [10,44,68]). This may be because wasps outcompete bees for these nesting structures [68] or because wasps outnumber bees in general (~115,000 wasp species versus ~20,000 bee species) [69,70]. We found both wasps and bees often provisioned the same timber cavity, in succession, which could indicate that nests were being usurped by either host [71]. However, we also found two ground-nesting bee species commonly emerged in high numbers from the same cob blocks. This suggests that interspecies cohabitation among nesting aggregations (or, more likely, their emerging offspring) may be higher than expected.

5. Conclusions

Our study shows that the composition of tropical solitary bee and wasp communities is vulnerable to change with landscape simplification. However, overall abundance and diversity of trap-nesting species may remain stable. Large, ground-nesting bee and spider-hunting wasp species were present in all landscapes, while those with specialist resource requirements and most (clepto) parasitoids were present only in complex landscapes. Most trap-nest studies in tropical agroecosystems are comparatively short-term and may not capture effects of landscape simplification on bee and wasp communities. This study highlights the need for multi-year studies incorporating nuanced measures such as composition with a focus on traits to detect changes bee and wasp populations.

Supplementary Materials

The following are available online at https://www.mdpi.com/2075-4450/11/12/853/s1, Table S1: Taxonomy of bees and wasps identified in this study, Data S1: Spreadsheet of all data used in this study, Script S1: R scripts used to analyse and visualise Data S1.

Author Contributions

Conceptualization, R.S.W., H.M.W. and S.D.L.; Methodology, R.S.W., C.F., T.J.S., H.M.W. and S.D.L.; Formal Analysis, R.S.W. and S.D.L.; Software, R.S.W. and S.D.L.; Validation, R.S.W., H.M.W. and S.D.L.; Investigation, R.S.W.; Resources, C.F. and H.M.W.; Data Curation, R.S.W.; Writing—Original Draft Preparation, R.S.W.; Writing—Review & Editing, R.S.W., H.M.W., S.D.L., C.J.B., C.F., T.J.S., B.F.K.; Visualization, R.S.W. and S.D.L.; Supervision, H.M.W. and S.D.L.; Project Administration, R.S.W. and H.M.W.; Funding Acquisition, R.S.W., H.M.W. and S.D.L. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the GENECOLOGY RESEARCH CENTRE, the UNIVERSITY OF THE SUNSHINE COAST and the FRIENDS OF THE MAROOCHY REGIONAL BUSHLAND BOTANIC GARDENS.

Acknowledgments

We thank the Australian Government Research Training Program and the Rotary Clubs of the Sunshine Coast for scholarships. Special thanks to the landholders: S. and A. Reed, D. Bell, M. Gowen and Macadamia Farm Management. We also thank Brittany Elliott, Chris Cannizarro, Ryan Newis and Wiebke Kaemper for their assistance.

Conflicts of Interest

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

References

  1. MacArthur, R.H.; MacArthur, J.W. On Bird Species Diversity. Ecology 1961, 42, 594–598. [Google Scholar] [CrossRef]
  2. Stein, A.; Gerstner, K.; Kreft, H. Environmental heterogeneity as a universal driver of species richness across taxa, biomes and spatial scales. Ecol. Lett. 2014, 17, 866–880. [Google Scholar] [CrossRef]
  3. IPBES. Summary for Policymakers of the Global Assessment Report on Biodiversity and Ecosystem Services of the Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services; IPBES Secretariat: Bonn, Germany, 2019. [Google Scholar]
  4. Landis, D.A. Designing agricultural landscapes for biodiversity-based ecosystem services. Basic Appl. Ecol. 2017, 18, 1–12. [Google Scholar] [CrossRef] [Green Version]
  5. Da Rocha-Filho, L.C.; Montagnana, P.C.; Boscolo, D.; Garófalo, C.A. Green patches among a grey patchwork: The importance of preserving natural habitats to harbour cavity-nesting bees and wasps (Hymenoptera) and their natural enemies in urban areas. Biodivers. Conserv. 2020. [Google Scholar] [CrossRef]
  6. Flores, L.M.A.; Zanette, L.R.S.; Araujo, F.S. Effects of habitat simplification on assemblages of cavity nesting bees and wasps in a semiarid neotropical conservation area. Biodivers. Conserv. 2018, 27, 311–328. [Google Scholar] [CrossRef]
  7. Jeliazkov, A.; Mimet, A.; Chargé, R.; Jiguet, F.; Devictor, V.; Chiron, F. Impacts of agricultural intensification on bird communities: New insights from a multi-level and multi-facet approach of biodiversity. Agric. Ecosyst. Environ. 2016, 216, 9–22. [Google Scholar] [CrossRef]
  8. Flynn, D.F.B.; Gogol-Prokurat, M.; Nogeire, T.; Molinari, N.; Richers, B.T.; Lin, B.B.; Simpson, N.; Mayfield, M.M.; DeClerck, F. Loss of functional diversity under land use intensification across multiple taxa. Ecol. Lett. 2009, 12, 22–33. [Google Scholar] [CrossRef]
  9. Seibold, S.; Gossner, M.M.; Simons, N.K.; Blüthgen, N.; Müller, J.; Ambarlı, D.; Ammer, C.; Bauhus, J.; Fischer, M.; Habel, J.C.; et al. Arthropod decline in grasslands and forests is associated with landscape-level drivers. Nature 2019, 574, 671–674. [Google Scholar] [CrossRef]
  10. Holzschuh, A.; Steffan-Dewenter, I.; Tscharntke, T. How do landscape composition and configuration, organic farming and fallow strips affect the diversity of bees, wasps and their parasitoids? J. Anim. Ecol. 2010, 79, 491–500. [Google Scholar] [CrossRef]
  11. Kennedy, C.M.; Lonsdorf, E.; Neel, M.C.; Williams, N.M.; Ricketts, T.H.; Winfree, R.; Bommarco, R.; Brittain, C.; Burley, A.L.; Cariveau, D.; et al. A global quantitative synthesis of local and landscape effects on wild bee pollinators in agroecosystems. Ecol. Lett. 2013, 16, 584–599. [Google Scholar] [CrossRef]
  12. Bianchi, F.J.J.A.; Booij, C.J.H.; Tscharntke, T. Sustainable pest regulation in agricultural landscapes: A review on landscape composition, biodiversity and natural pest control. Proc. R. Soc. B Biol. Sci. 2006, 273, 1715–1727. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  13. Connelly, H.; Poveda, K.; Loeb, G. Landscape simplification decreases wild bee pollination services to strawberry. Agric. Ecosyst. Environ. 2015, 211, 51–56. [Google Scholar] [CrossRef]
  14. Cunningham, S.A.; Schellhorn, N.A.; Marcora, A.; Batley, M. Movement and phenology of bees in a subtropical Australian agricultural landscape: Bees in an agricultural landscape. Austral Ecol. 2013, 38, 456–464. [Google Scholar] [CrossRef]
  15. Brosi, B.J.; Daily, G.C.; Shih, T.M.; Oviedo, F.; Durán, G. The effects of forest fragmentation on bee communities in tropical countryside: Bee communities and tropical forest fragmentation. J. Appl. Ecol. 2007, 45, 773–783. [Google Scholar] [CrossRef]
  16. Montoya-Pfeiffer, P.M.; Rodrigues, R.R.; Alves dos Santos, I. Bee pollinator functional responses and functional effects in restored tropical forests. Ecol. Appl. 2020, 30. [Google Scholar] [CrossRef] [PubMed]
  17. Smith, T.J.; Mayfield, M.M. The effect of habitat fragmentation on the bee visitor assemblages of three Australian tropical rainforest tree species. Ecol. Evol. 2018, 8, 8204–8216. [Google Scholar] [CrossRef] [Green Version]
  18. Williams, N.M.; Crone, E.E.; Roulston, T.a.H.; Minckley, R.L.; Packer, L.; Potts, S.G. Ecological and life-history traits predict bee species responses to environmental disturbances. Biol. Conserv. 2010, 143, 2280–2291. [Google Scholar] [CrossRef]
  19. Persson, A.S.; Rundlöf, M.; Clough, Y.; Smith, H.G. Bumble bees show trait-dependent vulnerability to landscape simplification. Biodivers. Conserv. 2015, 24, 3469–3489. [Google Scholar] [CrossRef]
  20. Sobek, S.; Tscharntke, T.; Scherber, C.; Schiele, S.; Steffan-Dewenter, I. Canopy vs. understory: Does tree diversity affect bee and wasp communities and their natural enemies across forest strata? For. Ecol. Manag. 2009, 258, 609–615. [Google Scholar] [CrossRef]
  21. Staab, M.; Pufal, G.; Tscharntke, T.; Klein, A.M. Trap nests for bees and wasps to analyse trophic interactions in changing environments—A systematic overview and user guide. Methods Ecol. Evol. 2018, 9, 2226–2239. [Google Scholar] [CrossRef]
  22. Tonietto, R.K.; Larkin, D.J. Habitat restoration benefits wild bees: A meta-analysis. J. Appl. Ecol. 2018, 55, 582–590. [Google Scholar] [CrossRef]
  23. Freitas, B.M.; Imperatriz-Fonseca, V.L.; Medina, L.M.; Kleinert, A.D.M.P.; Galetto, L.; Nates-Parra, G.; Quezada-Euán, J.J.G. Diversity, threats and conservation of native bees in the Neotropics. Apidologie 2009, 40, 332–346. [Google Scholar] [CrossRef] [Green Version]
  24. Michener, C.D. The Bees of the World, 2nd ed.; Johns Hopkins Press: Baltimore, Maryland, 2007. [Google Scholar]
  25. Matos, M.C.B.; Sousa-Souto, L.; Almeida, R.S.; Teodoro, A.V. Contrasting patterns of species richness and composition of solitary wasps and bees (Insecta: Hymenoptera) according to land-use. Biotropica 2013, 45, 73–79. [Google Scholar] [CrossRef]
  26. Tylianakis, J.M.; Klein, A.M.; Tscharntke, T. Spatiotemporal variation in the diversity of hymenoptera across a tropical habitat gradient. Ecology 2005, 86, 3296–3302. [Google Scholar] [CrossRef]
  27. Lassau, S.A.; Hochuli, D.F. Wasp community responses to habitat complexity in Sydney sandstone forests. Austral Ecol. 2005, 30, 179–187. [Google Scholar] [CrossRef]
  28. Gutiérrez-Chacón, C.; Dormann, C.F.; Klein, A.M. Forest-edge associated bees benefit from the proportion of tropical forest regardless of its edge length. Biol. Conserv. 2018, 220, 149–160. [Google Scholar] [CrossRef]
  29. Jha, S.; Vandermeer, J.H. Impacts of coffee agroforestry management on tropical bee communities. Biol. Conserv. 2010, 143, 1423–1431. [Google Scholar] [CrossRef]
  30. Carre, G.; Roche, P.; Chifflet, R.; Morison, N.; Bommarco, R.; Harrison-Cripps, J.; Krewenka, K.; Potts, S.G.; Roberts, S.P.; Rodet, G. Landscape context and habitat type as drivers of bee diversity in European annual crops. Agric. Ecosyst. Environ. 2009, 133, 40–47. [Google Scholar] [CrossRef]
  31. Feltham, H.; Park, K.; Minderman, J.; Goulson, D. Experimental evidence that wildflower strips increase pollinator visits to crops. Ecol. Evol. 2015, 5, 3523–3530. [Google Scholar] [CrossRef] [Green Version]
  32. Osorio-Canadas, S.; Arnan, X.; Bassols, E.; Vicens, N.; Bosch, J. Seasonal dynamics in a cavity-nesting bee-wasp community: Shifts in composition, functional diversity and host-parasitoid network structure. PLoS ONE 2018, 13, e0205854. [Google Scholar] [CrossRef] [Green Version]
  33. Ivanov, S. The nesting of Osmia rufa (L.)(Hymenoptera, Megachilidae) in the Crimea: Structure and composition of nests. Entomol. Rev. 2006, 86, 524–533. [Google Scholar] [CrossRef]
  34. MacIvor, J.S. Cavity-nest boxes for solitary bees: A century of design and research. Apidologie 2017, 48, 311–327. [Google Scholar] [CrossRef] [Green Version]
  35. Fortel, L.; Henry, M.; Guilbaud, L.; Mouret, H.; Vaissière, B.E. Use of human-made nesting structures by wild bees in an urban environment. J. Insect Conserv. 2016, 20, 239–253. [Google Scholar] [CrossRef] [Green Version]
  36. Hogendoorn, K.; Gross, C.L.; Sedgley, M.; Keller, M.A. Increased tomato yield through pollination by native Australian Amegilla chlorocyanea (Hymenoptera: Anthophoridae). J. Econ. Entomol. 2006, 99, 828–833. [Google Scholar] [CrossRef] [PubMed]
  37. Williams, K.J.; Ford, A.; Rosauer, D.F.; De Silva, N.; Mittermeier, R.; Bruce, C.; Larsen, F.W.; Margules, C. Forests of East Australia: The 35th biodiversity hotspot. In Biodiversity Hotspots; Springer: Berlin/Heidelberg, Germany, 2011; pp. 295–310. [Google Scholar]
  38. Kaluza, B.F.; Wallace, H.; Heard, T.A.; Klein, A.M.; Leonhardt, S.D. Urban gardens promote bee foraging over natural habitats and plantations. Ecol. Evol. 2016, 6, 1304–1316. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  39. Kaluza, B.F.; Wallace, H.; Keller, A.; Heard, T.A.; Jeffers, B.; Drescher, N.; Blüthgen, N.; Leonhardt, S.D. Generalist social bees maximize diversity intake in plant species-rich and resource-abundant environments. Ecosphere 2017, 8, e01758. [Google Scholar] [CrossRef] [Green Version]
  40. Greco, M.; Bell, M.; Spooner-Hart, R.; Holford, P. X-ray computerized tomography as a new method for monitoring Amegilla holmesi nest structures, nesting behaviour, and adult female activity. Entomol. Exp. Appl. 2006, 120, 71–76. [Google Scholar] [CrossRef]
  41. Makinson, J.C.; Threlfall, C.G.; Latty, T. Bee-friendly community gardens: Impact of environmental variables on the richness and abundance of exotic and native bees. Urban Ecosyst. 2017, 20, 463–476. [Google Scholar] [CrossRef]
  42. Palladini, J.D.; Maron, J.L. Reproduction and survival of a solitary bee along native and exotic floral resource gradients. Oecologia 2014, 176, 789–798. [Google Scholar] [CrossRef]
  43. Steckel, J.; Westphal, C.; Peters, M.K.; Bellach, M.; Rothenwoehrer, C.; Erasmi, S.; Scherber, C.; Tscharntke, T.; Steffan-Dewenter, I. Landscape composition and configuration differently affect trap-nesting bees, wasps and their antagonists. Biol. Conserv. 2014, 172, 56–64. [Google Scholar] [CrossRef]
  44. Diekötter, T.; Peter, F.; Jauker, B.; Wolters, V.; Jauker, F. Mass-flowering crops increase richness of cavity-nesting bees and wasps in modern agro-ecosystems. GCB Bioenergy 2014, 6, 219–226. [Google Scholar] [CrossRef]
  45. R Development Core Team. R: A Language and Environment for Statistical Computing; R Foundation for Statistical Computing: Vienna, Austria, 2018. [Google Scholar]
  46. Oksanen, J.; Blanchet, F.G.; Friendly, M.; Kindt, R.; Legendre, P.; McGlinn, D.; Minchin, P.R.; O’Hara, R.B.; Simpson, G.L.; Solymos, P.; et al. Vegan: Community Ecology Package; CRAN: Vienna, Austria, 2019. [Google Scholar]
  47. Anderson, M.J. A new method for non-parametric multivariate analysis of variance. Austral Ecol. 2001, 26, 32–46. [Google Scholar]
  48. Harrison, X.A. Using observation-level random effects to model overdispersion in count data in ecology and evolution. PeerJ 2014, 2, e616. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  49. Bolker, B.M.; Brooks, M.E.; Clark, C.J.; Geange, S.W.; Poulsen, J.R.; Stevens, M.H.H.; White, J.S.S. Generalized linear mixed models: A practical guide for ecology and evolution. Trends Ecol. Evol. 2009, 24, 127–135. [Google Scholar] [CrossRef] [PubMed]
  50. Barton, K. MuMIn: Multi-Model Inference; R Package Version 1.43.6; CRAN: Vienna, Austria, 2019. [Google Scholar]
  51. Hothorn, T.; Bretz, F.; Westfall, P.; Heiberger, R.M.; Schuetzenmeister, A.; Scheibe, S. Multcomp: Simultaneous Inference in General Parametric Models; 1.4-14; CRAN: Vienna, Austria, 2020. [Google Scholar]
  52. Stangler, E.S.; Hanson, P.E.; Steffan-Dewenter, I. Interactive effects of habitat fragmentation and microclimate on trap-nesting Hymenoptera and their trophic interactions in small secondary rainforest remnants. Biodivers. Conserv. 2015, 24, 563–577. [Google Scholar] [CrossRef]
  53. Vehviläinen, H.; Koricheva, J.; Ruohomäki, K. Tree species diversity influences herbivore abundance and damage: Meta-analysis of long-term forest experiments. Oecologia 2007, 152, 287–298. [Google Scholar] [CrossRef]
  54. Buschini, M.L.T.; Caldas, T.R.; Borba, N.A.; Brescovit, A.D. Spiders used as prey by the hunting wasp Trypoxylon (Trypargilum) agamemnon Richards (Hymenoptera: Crabronidae). Zool. Stud. 2010, 49, 169–175. [Google Scholar]
  55. Jauker, B.; Krauss, J.; Jauker, F.; Steffan-Dewenter, I. Linking life history traits to pollinator loss in fragmented calcareous grasslands. Landsc. Ecol. 2013, 28, 107–120. [Google Scholar] [CrossRef] [Green Version]
  56. Fabian, Y.; Sandau, N.; Bruggisser, O.T.; Aebi, A.; Kehrli, P.; Rohr, R.P.; Naisbit, R.E.; Bersier, L.F. Plant diversity in a nutshell: Testing for small-scale effects on trap nesting wild bees and wasps. Ecosphere 2014, 5, art18. [Google Scholar] [CrossRef] [Green Version]
  57. Ebeling, A.; Klein, A.M.; Weisser, W.W.; Tscharntke, T. Multitrophic effects of experimental changes in plant diversity on cavity-nesting bees, wasps, and their parasitoids. Oecologia 2012, 169, 453–465. [Google Scholar] [CrossRef]
  58. Bommarco, R.; Biesmeijer, J.C.; Meyer, B.; Potts, S.G.; Pöyry, J.; Roberts, S.P.M.; Steffan-Dewenter, I.; Öckinger, E. Dispersal capacity and diet breadth modify the response of wild bees to habitat loss. Proc. R. Soc. B Biol. Sci. 2010, 277, 2075–2082. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  59. Wcislo, W.T.; Cane, J.H. Floral Resource Utilization by Solitary Bees (Hymenoptera: Apoidea) and Exploitation of Their Stored Foods by Natural Enemies. Annu. Rev. Entomol. 1996, 41, 257–286. [Google Scholar] [CrossRef] [PubMed]
  60. Williams, N.M. Use of novel pollen species by specialist and generalist solitary bees (Hymenoptera: Megachilidae). Oecologia 2003, 134, 228–237. [Google Scholar] [CrossRef] [PubMed]
  61. Vanderplanck, M.; Vereecken, N.J.; Grumiau, L.; Esposito, F.; Lognay, G.; Wattiez, R.; Michez, D. The importance of pollen chemistry in evolutionary host shifts of bees. Sci. Rep. 2017, 7, 43058. [Google Scholar] [CrossRef] [Green Version]
  62. Ogilvie, J.E.; Forrest, J.R.K. Interactions between bee foraging and floral resource phenology shape bee populations and communities. Curr. Opin. Insect Sci. 2017, 21, 75–82. [Google Scholar] [CrossRef]
  63. Nicholls, C.I.; Altieri, M.A. Plant biodiversity enhances bees and other insect pollinators in agroecosystems. A review. Agron. Sustain. Dev. 2013, 33, 257–274. [Google Scholar] [CrossRef] [Green Version]
  64. Holzschuh, A.; Dormann, C.F.; Tscharntke, T.; Steffan-Dewenter, I. Mass-flowering crops enhance wild bee abundance. Oecologia 2013, 172, 477–484. [Google Scholar] [CrossRef] [Green Version]
  65. Weiner, C.N.; Werner, M.; Linsenmair, K.E.; Blüthgen, N. Land-use impacts on plant–pollinator networks: Interaction strength and specialization predict pollinator declines. Ecology 2014, 95, 466–474. [Google Scholar] [CrossRef]
  66. Vizentin-Bugoni, J.; Maruyama, P.K.; de Souza, C.S.; Ollerton, J.; Rech, A.R.; Sazima, M. Plant-Pollinator Networks in the Tropics: A Review. In Ecological Networks in the Tropics; Dáttilo, W., Rico-Gray, V., Eds.; Springer International Publishing: Cham, Germany, 2018; pp. 73–91. [Google Scholar]
  67. Hoffmann, U.S.; Jauker, F.; Lanzen, J.; Warzecha, D.; Wolters, V.; Diekötter, T. Prey-dependent benefits of sown wildflower strips on solitary wasps in agroecosystems. Insect Conserv. Divers. 2018, 11, 42–49. [Google Scholar] [CrossRef]
  68. MacIvor, J.S.; Packer, L. ‘Bee Hotels’ as Tools for Native Pollinator Conservation: A Premature Verdict? PLoS ONE 2015, 10, e0122126. [Google Scholar] [CrossRef]
  69. LaSalle, J.; Gauld, I.D. Parasitic hymenoptera and the biodiversity crisis. Redia 1992, 74, 315–344. [Google Scholar]
  70. Ascher, J.S.; Pickering, J. Discover Life Bee Species Guide and World Checklist (Hymenoptera: Apoidea: Anthophila). Available online: www.discoverlife.org/20/q?search=Apoidea (accessed on 3 November 2020).
  71. Wcislo, W.; Fewell, J.H. Sociality in Bees. In Comparative Social Evolution; Rubenstein, D.R., Abbot, P., Eds.; Cambridge University Press: Cambridge, UK, 2017; pp. 50–83. [Google Scholar]
Insects 11 00853 g001 550
Figure 1. Study landscapes and trap nests. View of a forest (A) and orchard (B) landscape used in this study; habitat structure within a forest (C) and orchard (D) site; and an example of the trap nest units deployed in all sites (E) with cob blocks, pithy stems, short timber (100 mm long × 6 mm diameter) and long timber (150 mm long × 8 mm diameter) trap nests.
Figure 1. Study landscapes and trap nests. View of a forest (A) and orchard (B) landscape used in this study; habitat structure within a forest (C) and orchard (D) site; and an example of the trap nest units deployed in all sites (E) with cob blocks, pithy stems, short timber (100 mm long × 6 mm diameter) and long timber (150 mm long × 8 mm diameter) trap nests.
Insects 11 00853 g001
Insects 11 00853 g002 550
Figure 2. Changes of bee (A) and wasp (B) communities with landscape simplification and seasonal variation. Ellipses and points show forest (green) and orchard (orange) communities (standard deviation of point scores for groups of land use). Each point represents one trap nest unit in one season in one year (NMDS, Bray distance, 999 permutations). Vectors show direction of selected significant environmental factors. Bee community composition (A) differed with land use, but also distance to forest, forest cover, and plant richness. Composition of wasp communities (B) similarly differed with land use, percentage of forest cover, and distance to forest, but not plant richness.
Figure 2. Changes of bee (A) and wasp (B) communities with landscape simplification and seasonal variation. Ellipses and points show forest (green) and orchard (orange) communities (standard deviation of point scores for groups of land use). Each point represents one trap nest unit in one season in one year (NMDS, Bray distance, 999 permutations). Vectors show direction of selected significant environmental factors. Bee community composition (A) differed with land use, but also distance to forest, forest cover, and plant richness. Composition of wasp communities (B) similarly differed with land use, percentage of forest cover, and distance to forest, but not plant richness.
Insects 11 00853 g002
Table
Table 1. Landscape characteristics of study sites.
Table 1. Landscape characteristics of study sites.
Land UseSitePlant Species RichnessForest Cover (%)Orchard Cover (%)Distance to Forest (m)
ForestF15396.3600
F213989.7900
F315597.5200
F417375.0620.650
OrchardP251092.04640
P31343.5393.21212
P46916.7480.08177
Table
Table 2. Taxa identified in trap nests and their abundance in forests (“F”) and orchards (“O”). Some trap-nesting bees and wasps were found only in forests or orchards. Symbology shows aboveground nesting a, belowground nesting b, cleptoparasitic c.p., parasitic p, cellophane c, leafcutter l and resin-collecting r species. Abundance indicates total and mean (+/− se) number of adult emergents for ground-nesting bees and of brood cells for cavity-nesting bees and wasps. All specimens are determined to species and labelled by taxa names or letters “A” to “J” after “sp.” for unnamed taxa.
Table 2. Taxa identified in trap nests and their abundance in forests (“F”) and orchards (“O”). Some trap-nesting bees and wasps were found only in forests or orchards. Symbology shows aboveground nesting a, belowground nesting b, cleptoparasitic c.p., parasitic p, cellophane c, leafcutter l and resin-collecting r species. Abundance indicates total and mean (+/− se) number of adult emergents for ground-nesting bees and of brood cells for cavity-nesting bees and wasps. All specimens are determined to species and labelled by taxa names or letters “A” to “J” after “sp.” for unnamed taxa.
Family
(% of Nests)		SpeciesAbundanceNumber of Nests
Total BroodPer Nest
Mean ± SE		FOTotal
Bees
Apidae (25%)Amegilla (Zonamegilla) adelaidaeb855.67 (1.28)9615
Amegilla (Zonamegilla) sp. B b42.00 (1.00)112
Thyreus cf. caeruleopunctatus b,c.p.11.00 (NA)011
Thyreus nitidulusb,c.p.21.00 (0.00)202
Colletidae (26%)Hylaeus (Euprosopoides) ruficeps ruficepsa,c11.00 (NA)101
Hylaeus (Hylaeorhiza) nubilosusa,c376.17 (2.02)426
Hyleoides concinnaa,c134.33 (1.77)303
Pachyprosopis (Parapachyprosopis) angophoraeb82.67 (0.88)033
Pachyprosopis (Parapachyprosopis) indicansb607.50 (2.91)718
Megachilidae (49%)Megachile (Callomegachile) mystacaenaa,r693.00 (0.50)23023
Megachile (Eutricharaea) simplexa,l494.45 (0.87)2911
Megachile (Rhodomegachile) deaniia,r124.00 (0.00)123
Megachile mackayensisa,r31.50 (0.50)202
Wasps
Chrysididae (3%)Primeuchroeus sp. a,p41.33 (0.33)033
Crabronidae (57%)Pison sp. A a255.00 (1.31)145
Pison sp. B a923.54 (0.30)131326
Pison sp. C a92.25 (0.63)134
Pison sp. D a777.70 (0.60)01010
Pison sp. E a935.81 (0.68)16016
Pison sp. F a113.67 (0.88)033
Pison sp. G a11.00 (NA)101
Pison sp. H a11.00 (NA)101
Pison sp. I a11.00 (NA)011
Pison sp. J a55.00 (NA)101
Gasteruptiidae (4%)Gasteruption sp. A a,p 41.00 (0.00)404
Gasteruption sp. B a,p 11.00 (NA)101
Mutillidae (4%)Mutillidae sp. A a,p 21.00 (0.00)112
Mutillidae sp. B a,p 11.00 (NA)101
Mutillidae sp. C a,p11.00 (NA)101
Mutillidae sp. D a,p11.00 (NA)101
Perilampidae (2%)Perilampus sp. a,p21.00 (0.00)112
Pompilidae (17%)Fabriogenia sp. A a72.33 (1.33)123
Fabriogenia sp. B a644.57 (0.66)10414
Fabriogenia sp. C a84.00 (3.00)202
Irenangelus sp. a,c.p.11.00 (NA)101
Sphecidae (3%)Isodontia sp. a31.00 (0.00)033
Vespidae (12%)Anterhynchium (Epiodynerus) nigrocinctusa315.17 (0.40)066
Anterhynchium (Epiodynerus) tamarinuma41.33 (0.33)033
Eumeninae sp. B a11.00 (NA)101
Eumeninae sp. D a168.00 (5.00)202
Paralastor sp. a42.00 (1.00)202
Sub total 814 11882200
Bee nests identified to (sub)family230 531568
Wasp nests identified to (sub)family48 8311
Unidentified wasp nests1159 170113283
Total 2251 349213562
Table
Table 3. Environmental effects on composition of bee and wasp communities. Bee communities were influenced by all environmental factors except rainfall. Wasp communities were influenced by all environmental factors except plant richness. Parameters reported are from permutational mANOVAs and non-metrical multidimensional scaling (NMDS) based on Bray–Curtis similarity distances between abundance of each species per trap nest unit, per season (999 permutations, 3 dimensions).
Table 3. Environmental effects on composition of bee and wasp communities. Bee communities were influenced by all environmental factors except rainfall. Wasp communities were influenced by all environmental factors except plant richness. Parameters reported are from permutational mANOVAs and non-metrical multidimensional scaling (NMDS) based on Bray–Curtis similarity distances between abundance of each species per trap nest unit, per season (999 permutations, 3 dimensions).
CommunityStressExplanatory VariablesFR2p
Bees0.14Season1.8090.140.009
Temperature4.3520.110.001
Rainfall1.5440.040.121
Land use3.1350.080.003
Forest cover3.8040.10.001
Distance to forest3.0510.080.001
Plant richness3.3280.090.002
Wasps0.2Season1.7870.10.004
Temperature2.0410.040.017
Rainfall2.4080.040.007
Land use2.7270.050.002
Forest cover2.7790.050.002
Distance to forest2.8260.050.004
Plant richness0.9940.020.439
Table
Table 4. Contributions of bee and wasp species to forest and orchard communities. Proportions represent each species’ contribution to the total community dissimilarity between forests and orchards. Most of the dissimilarity between land uses is due to just three bee species (62%, cumulative): M. simplex, A. adelaidae, and M. mystacaena (found only in forests). For wasps, most dissimilarity is contributed by four wasp species (60%): Pison sp. B, Pison sp. E (found only in forests), Fabriogenia sp. B and Pison sp. D (found only in orchards).
Table 4. Contributions of bee and wasp species to forest and orchard communities. Proportions represent each species’ contribution to the total community dissimilarity between forests and orchards. Most of the dissimilarity between land uses is due to just three bee species (62%, cumulative): M. simplex, A. adelaidae, and M. mystacaena (found only in forests). For wasps, most dissimilarity is contributed by four wasp species (60%): Pison sp. B, Pison sp. E (found only in forests), Fabriogenia sp. B and Pison sp. D (found only in orchards).
SpeciesProportion
MeanCumulative
Bees
Megachile (Eutricharaea) simplex0.23560.2515
Amegilla (Zonamegilla) adelaidae0.19660.4613
Megachile (Callomegachile) mystacaena0.15220.6237
Pachyprosopis (Parapachyprosopis) indicans0.10320.7339
Hylaeus (Hylaeorhiza) nubilosus0.09380.8340
Megachile (Rhodomegachile) deanii0.06580.9042
Pachyprosopis (Parapachyprosopis) angophorae0.02980.9361
Hyleoides concinna0.02260.9602
Amegilla (Zonamegilla) sp. B0.01640.9778
Hylaeus (Euprosopoides) ruficeps ruficeps0.00640.9846
Megachile mackayensis0.00520.9902
Thyreus cf. caeruleopunctatus0.00490.9955
Thyreus nitidulus0.00421.0000
Wasps
Pison sp. B0.18950.2049
Pison sp. E0.15620.3738
Fabriogenia sp. B0.12530.5093
Pison sp. D0.09170.6084
Anterhynchium (Epiodynerus) nigrocinctus0.06770.6817
Pison sp. A0.05600.7423
Pison sp. C0.03280.7778
Pison sp. F0.02980.8101
Fabriogenia sp. A0.02590.8381
Fabriogenia sp. C0.01950.8592
Eumeninae sp. D0.01710.8778
Isodontia sp.0.01660.8957
Gasteruption sp. A0.01360.9105
Paralastor sp.0.01100.9225
Primeuchroeus sp.0.01050.9339
Anterhynchium (Epiodynerus) tamarinum0.01050.9453
Mutillidae sp. A0.00900.9551
Pison sp. J0.00890.9647
Irenangelus sp.0.00610.9714
Gasteruption sp. B0.00480.9819
Eumeninae sp. B0.00480.9766
Perilampus sp.0.00360.9858
Pison sp. G0.00280.9889
Pison sp. H0.00280.9920
Mutillidae sp. B0.00240.9947
Mutillidae sp. C0.00160.9965
Mutillidae sp. D0.00160.9983
Pison sp. I0.00151.0000
Table
Table 5. Species richness, diversity, and abundance of bees and wasps in response to landscape simplification and season. Explanatory power of most parsimonious GLMMs including statistically significant variables is shown as variance of fixed (marginal R²) and random effects (conditional R²). Significance levels of variables were assessed by likelihood-ratio tests (LRT) against null models (i.e., random factors only). Below-ground bee abundance is modelled as a presence-absence (binary) response. There was no significant model for bee richness, wasp richness, or wasp abundance.
Table 5. Species richness, diversity, and abundance of bees and wasps in response to landscape simplification and season. Explanatory power of most parsimonious GLMMs including statistically significant variables is shown as variance of fixed (marginal R²) and random effects (conditional R²). Significance levels of variables were assessed by likelihood-ratio tests (LRT) against null models (i.e., random factors only). Below-ground bee abundance is modelled as a presence-absence (binary) response. There was no significant model for bee richness, wasp richness, or wasp abundance.
ResponseExplanatory∆ R2LRTTukey Post-hoc
MarginalConditionalX2dfpLevels (Direction)p
Bee diversitySeason0.150.408.01830.0456Spring > Autumn0.0461
Above-ground bee abundanceRainfall0.190.9910.28910.0013(+)
Season0.190.9815.01630.0018Summer > Autumn0.0114
Below-ground bee abundanceRainfall 0.160.165.062 10.0244(+)
Season0.960.9943.8013<0.0001Spring > Autumn<0.0001
Summer > Autumn<0.0001
Summer > Spring<0.0001
Winter > Spring<0.0001
Winter > Summer <0.0001
Winter > Autumn<0.0001
Wasp diversitySeason0.200.3112.24930.0065Summer > Autumn0.0033
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Wilson, R.S.; Leonhardt, S.D.; Burwell, C.J.; Fuller, C.; Smith, T.J.; Kaluza, B.F.; Wallace, H.M. Landscape Simplification Modifies Trap-Nesting Bee and Wasp Communities in the Subtropics. Insects 2020, 11, 853. https://doi.org/10.3390/insects11120853

AMA Style

Wilson RS, Leonhardt SD, Burwell CJ, Fuller C, Smith TJ, Kaluza BF, Wallace HM. Landscape Simplification Modifies Trap-Nesting Bee and Wasp Communities in the Subtropics. Insects. 2020; 11(12):853. https://doi.org/10.3390/insects11120853

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Wilson, Rachele S., Sara D. Leonhardt, Chris J. Burwell, Chris Fuller, Tobias J. Smith, Benjamin F. Kaluza, and Helen M. Wallace. 2020. "Landscape Simplification Modifies Trap-Nesting Bee and Wasp Communities in the Subtropics" Insects 11, no. 12: 853. https://doi.org/10.3390/insects11120853

APA Style

Wilson, R. S., Leonhardt, S. D., Burwell, C. J., Fuller, C., Smith, T. J., Kaluza, B. F., & Wallace, H. M. (2020). Landscape Simplification Modifies Trap-Nesting Bee and Wasp Communities in the Subtropics. Insects, 11(12), 853. https://doi.org/10.3390/insects11120853

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