Gut Bacterial Diversity of Insecticide-Susceptible and Insecticide-Resistant Megalurothrips usitatus (Thysanoptera: Thripidae) and Elucidation of Their Putative Functional Roles
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Insect Populations
2.2. Insecticides and Toxicological Bioassay
2.3. Extraction of DNA from the Guts of the IS and IR M. usitatus
2.4. Amplicon Library Construction and Illumina Sequencing
2.5. Processing of the Sequence Reads and Data Analysis
3. Results
3.1. Toxicological Bioassay
3.2. Illumina MiSeq Metagenomic Data and Taxonomic Assignments
3.3. Functional Analysis of Intestinal Bacteria of Two Populations of M. usitatus after Infection with B. brongniartii Isolate SB010
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Insecticide | Population | Slope ± SE | LC50 (95% CL) a | LCR50 (95%CL) b |
---|---|---|---|---|
Acetamiprid- | IS | 1.00 ± 0.18 | 8.63 (4.18–10.57) | 138.04 (172.24–186.48) * |
IR | 1.57 ± 0.17 | 1191.28 (719.97–1971.14) | ||
B. brongniartii | IS | 0.38 ± 2.70 | 1.2 × 106 (2.5 × 105–6.0 × 106) | 55.62 (10.26–30,150.03) * |
IR | 0.31 ± 2.56 | 6.8 × 107 (2.6 × 106–1.8 × 109) |
Sample | Raw Tags | Clean Tags | Effective Tags | Effective Ratio (%) | OTUs (Individual) |
---|---|---|---|---|---|
S-CK | 102,074 | 96,541 | 92,964 | 74 | 191 |
B1 | 109,466 | 105,369 | 99,172 | 74 | 81 |
B2 | 109,422 | 104,815 | 98,539 | 73 | 92 |
B3 | 104,250 | 100,345 | 94,579 | 74 | 93 |
R-CK | 106,162 | 101,810 | 95,872 | 73 | 95 |
D1 | 105,975 | 102,716 | 98,807 | 76 | 142 |
D2 | 102,590 | 98,920 | 93,522 | 74 | 76 |
D3 | 103,351 | 99,454 | 94,125 | 74 | 76 |
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Zhu, B.; Chen, Y.; Zhou, C.; Li, H.; Ali, S.; Wu, J. Gut Bacterial Diversity of Insecticide-Susceptible and Insecticide-Resistant Megalurothrips usitatus (Thysanoptera: Thripidae) and Elucidation of Their Putative Functional Roles. Insects 2023, 14, 669. https://doi.org/10.3390/insects14080669
Zhu B, Chen Y, Zhou C, Li H, Ali S, Wu J. Gut Bacterial Diversity of Insecticide-Susceptible and Insecticide-Resistant Megalurothrips usitatus (Thysanoptera: Thripidae) and Elucidation of Their Putative Functional Roles. Insects. 2023; 14(8):669. https://doi.org/10.3390/insects14080669
Chicago/Turabian StyleZhu, Bifeng, Yueyin Chen, Chenyan Zhou, Haolong Li, Shaukat Ali, and Jianhui Wu. 2023. "Gut Bacterial Diversity of Insecticide-Susceptible and Insecticide-Resistant Megalurothrips usitatus (Thysanoptera: Thripidae) and Elucidation of Their Putative Functional Roles" Insects 14, no. 8: 669. https://doi.org/10.3390/insects14080669