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Insects, Volume 14, Issue 8

2023 August - 51 articles

Cover Story: We developed a small-scale rearing system to support functional genomic studies of the western corn rootworm (WCR), Diabrotica virgifera virgifera, a major pest of corn. Although protocols were available for rearing WCR in the laboratory for use in pesticide trials and testing resistance to transgenic crops, they were not optimal for functional genomic studies. Therefore, we developed a specialized rearing system that allows manipulation of WCR at different life stages and is efficient for rearing multiple strains on a small scale. Importantly, our rearing system supports other protocols we have established, namely microinjection of pre-cellular WCR embryos for the purpose of germline transformation or CRISPR/Cas9-based genome editing. We also outline the basic steps necessary for performing each of the related tasks, such as screening and handling WCR at various life stages. View this paper
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Articles (51)

  • Article
  • Open Access
5 Citations
2,543 Views
14 Pages

Breeding Polyommatus icarus Serves as a Large-Scale and Environmentally Friendly Source of Precisely Tuned Photonic Nanoarchitectures

  • Gábor Piszter,
  • Zsolt Bálint,
  • Krisztián Kertész,
  • Lajos Szatmári,
  • Gábor Sramkó and
  • László Péter Biró

18 August 2023

The colour of the butterfly wing serves as an important sexual and species-specific signal. Some species produce structural colouration by developing wing scales with photonic nanoarchitectures. These nanostructures are highly conservative, allowing...

  • Article
  • Open Access
2 Citations
2,093 Views
10 Pages

18 August 2023

At present, sexual attractants mainly control insect populations by killing males. However, the effect of sex attractants may be limited by the mating ability of the attracted insects. The Oriental fruit fly, Bactrocera dorsalis (Hendel), has a stron...

  • Article
  • Open Access
5 Citations
2,651 Views
20 Pages

Evaluation of the Effect of the ENSO Cycle on the Distribution Potential of the Genus Anastrepha of Horticultural Importance in the Neotropics and Panama

  • Arturo Batista Degracia,
  • Julián Ávila Jiménez,
  • Anovel Barba Alvarado,
  • Randy Atencio Valdespino and
  • Mariano Altamiranda-Saavedra

18 August 2023

Climate variability has made us change our perspective on the study of insect pests and pest insects, focusing on preserving or maintaining efficient production systems in the world economy. The four species of the genus Anastrepha were selected for...

  • Article
  • Open Access
8 Citations
3,246 Views
15 Pages

A New Approach for Detecting Sublethal Effects of Neonicotinoids on Bumblebees Using Optical Sensor Technology

  • Vasileia Chatzaki,
  • Marta Montoro,
  • Rámi El-Rashid,
  • Annette Bruun Jensen and
  • Antoine Lecocq

17 August 2023

Among insects, bees are important pollinators, providing many vital ecosystem services. The recent pollinator decline is threatening both their diversity and abundance. One of the main drivers of this decline is the extensive use of pesticides. Neoni...

  • Communication
  • Open Access
1 Citations
1,842 Views
10 Pages

Allometry of Defense: Predator Shift Alters Ontogenetic Growth Patterns in an Antipredator Trait

  • Bin Jiang,
  • Yu Yao,
  • Rüdiger Mauersberger and
  • Dirk J. Mikolajewski

17 August 2023

Predation is a major factor driving prey trait diversification and promoting ecological speciation. Consequently, antipredator traits are widely studied among prey species. However, comparative studies that examine how different predators shape the o...

  • Review
  • Open Access
17 Citations
7,264 Views
13 Pages

16 August 2023

Insect growth is interrupted by molts, during which the insect develops a new exoskeleton. The exoskeleton confers protection and undergoes shedding between each developmental stage through an evolutionarily conserved and ordered sequence of behavior...

  • Article
  • Open Access
9 Citations
2,953 Views
13 Pages

14 August 2023

Many insects rely on ancient symbiotic bacterial associations for essential nutrition. Auchenorrhyncha commonly harbor two obligate symbionts: Sulcia (Bacteroidetes) and a proteobacterial partner that supplies essential amino acids lacking in their p...

  • Article
  • Open Access
3 Citations
2,153 Views
16 Pages

14 August 2023

The hematopoietic system plays a crucial role in immune defense response and normal development, and it is regulated by various factors from other tissues. The dysregulation of hematopoiesis is associated with melanotic mass formation; however, the m...

  • Article
  • Open Access
1 Citations
3,110 Views
17 Pages

Development of a Diet Production System for Conopomorpha cramerella (Lepidoptera: Gracillariidae), a Major Cocoa Production Pest in Southeast Asia and the Pacific Islands

  • Jerome Niogret,
  • Anisah Binti Savantil,
  • Arni Ekayanti,
  • Mavis Peter Jaus,
  • Wulan Wulan,
  • Elviah Mitzo,
  • Jean-Philippe Marelli and
  • Desmond Conlong

14 August 2023

The development of artificial diets for the cocoa pod borer Conopomorpha cramerella, a major pest of cocoa plants, has undergone significant advancements. In this study, we present the success rates of two diet formulations, MM1 and MM4, which have b...

  • Article
  • Open Access
3 Citations
3,638 Views
15 Pages

14 August 2023

Circadian clocks regulate ecologically important complex behaviors in honey bees, but it is not clear whether similar capacities exist in other species of bees. One key behavior influenced by circadian clocks is time-memory, which enables foraging be...

  • Article
  • Open Access
7 Citations
3,229 Views
13 Pages

Atg2 Regulates Cellular and Humoral Immunity in Drosophila

  • Bo Qin,
  • Shichao Yu,
  • Qiming Chen and
  • Li Hua Jin

14 August 2023

Autophagy is a process that promotes the lysosomal degradation of cytoplasmic proteins and is highly conserved in eukaryotic organisms. Autophagy maintains homeostasis in organisms and regulates multiple developmental processes, and autophagy disrupt...

  • Brief Report
  • Open Access
5 Citations
2,438 Views
11 Pages

14 August 2023

During field tests implemented in Transcarpathia (West Ukraine) in 2015, 6501 specimens belonging to 26 Brachyceran fly families were collected with traps baited with generic lures (originally developed for noctuid moths) based on fermenting liquid a...

  • Article
  • Open Access
2,594 Views
15 Pages

14 August 2023

Membracis mexicana (Hemiptera: Membracidae) is distributed in four biogeographic provinces of Mexico. Field observations indicate that there are different forms of this species, but the distribution of the phenotype and the genetic variation of this...

  • Article
  • Open Access
3 Citations
7,681 Views
14 Pages

A Rapid Method for Measuring In Vitro Growth in Entomopathogenic Fungi

  • Anna R. Slowik,
  • Helen Hesketh,
  • Steven M. Sait and
  • Henrik H. de Fine Licht

13 August 2023

Quantifying the growth of entomopathogenic fungi is crucial for understanding their virulence and pathogenic potential. Traditional methods for determining growth, such as biomass determination or colony growth area, are time-consuming and quantitati...

  • Feature Paper
  • Article
  • Open Access
6 Citations
3,954 Views
18 Pages

11 August 2023

As moths are vital components of ecosystems and serve as important bioindicators, understanding the dynamics of their communities and the factors influencing these dynamics, such as anthropogenic impacts, is crucial to understand the ecological proce...

  • Feature Paper
  • Article
  • Open Access
11 Citations
3,635 Views
19 Pages

Transcriptomic Analysis Reveals the Impact of the Biopesticide Metarhizium anisopliae on the Immune System of Major Workers in Solenopsis invicta

  • Hongxin Wu,
  • Yating Xu,
  • Junaid Zafar,
  • Surajit De Mandal,
  • Liangjie Lin,
  • Yongyue Lu,
  • Fengliang Jin,
  • Rui Pang and
  • Xiaoxia Xu

11 August 2023

The red imported fire ant (Solenopsis invicta Buren, 1972) is a globally significant invasive species, causing extensive agricultural, human health, and biodiversity damage amounting to billions of dollars worldwide. The pathogenic fungus Metarhizium...

  • Article
  • Open Access
3 Citations
2,261 Views
13 Pages

MicroRNA Expression Prior to Biting in a Vector Mosquito Anticipates Physiological Processes Related to Energy Utilization, Reproduction and Immunity

  • Sarah Marzec,
  • Alden Siperstein,
  • Angela Zhou,
  • Christina M. Holzapfel,
  • William E. Bradshaw,
  • Megan E. Meuti and
  • Peter A. Armbruster

10 August 2023

Understanding the molecular and physiological processes underlying biting behavior in vector mosquitoes has important implications for developing novel strategies to suppress disease transmission. Here, we conduct small-RNA sequencing and qRT-PCR to...

  • Article
  • Open Access
9 Citations
3,079 Views
13 Pages

10 August 2023

Eucryptorrhynchus scrobiculatus and E. brandti are the main borers of Ailanthus altissima, causing serious economic and ecological losses. The external morphology and internal ultrastructure of the compound eyes of two related weevils were investigat...

  • Article
  • Open Access
10 Citations
3,617 Views
14 Pages

Enhanced YOLOv5 Object Detection Algorithm for Accurate Detection of Adult Rhynchophorus ferrugineus

  • Shuai Wu,
  • Jianping Wang,
  • Li Liu,
  • Danyang Chen,
  • Huimin Lu,
  • Chao Xu,
  • Rui Hao,
  • Zhao Li and
  • Qingxuan Wang

9 August 2023

The red palm weevil (RPW, Rhynchophorus ferrugineus) is an invasive and highly destructive pest that poses a serious threat to palm plants. To improve the efficiency of adult RPWs’ management, an enhanced YOLOv5 object detection algorithm based...

  • Article
  • Open Access
7 Citations
2,545 Views
13 Pages

Novel Lactone-Based Insecticides and Drosophila suzukii Management: Synthesis, Potential Action Mechanisms and Selectivity for Non-Target Parasitoids

  • Javier G. Mantilla Afanador,
  • Sabrina H. C. Araujo,
  • Milena G. Teixeira,
  • Dayane T. Lopes,
  • Cristiane I. Cerceau,
  • Felipe Andreazza,
  • Daiana C. Oliveira,
  • Daniel Bernardi,
  • Wellington S. Moura and
  • Eugenio E. Oliveira
  • + 4 authors

9 August 2023

Drosophila suzukii, an invasive insect pest, poses a significant threat to various fruit crops. The use of broad-spectrum insecticides to control this pest can reduce the effectiveness of biological control agents, such as the parasitoid Trichopria a...

  • Article
  • Open Access
3 Citations
3,120 Views
12 Pages

First Records of Heartbeats via ECG in a Stingless Bee, Melipona flavolineata (Apidae, Meliponini), during Contention Stress Using Isoflurane as an Anesthetic

  • Felipe Andrés León Contrera,
  • Bárbara dos Santos Conceição Lopes,
  • Clarissa Araújo da Paz,
  • Maria Klara Otake Hamoy,
  • Murilo Farias dos Santos,
  • Gabriela Brito Barbosa,
  • Anthony Lucas Gurgel do Amaral,
  • Luiz Henrique Barbosa de Pinho and
  • Moisés Hamoy

8 August 2023

The hemodynamic activity of Melipona flavolineata workers was evaluated during restraint stress for a period of 30 min. The observed parameters were power variation in the elapsed time, and subsequently, six periods of one second were divided and cal...

  • Article
  • Open Access
12 Citations
2,160 Views
14 Pages

Different Tea Germplasms Distinctly Influence the Adaptability of Toxoptera aurantii (Hemiptera: Aphididae)

  • Changhao Lu,
  • Ni Shen,
  • Wenbin Jiang,
  • Bi Xie,
  • Runa Zhao,
  • Guolan Zhou,
  • Degang Zhao,
  • Yingqin He and
  • Wenlong Chen

7 August 2023

Aphids are typical phloem-sucking insect pests. A good understanding regarding their feeding behavior and population dynamics are critical for evaluating host adaptation and screening of aphid-resistant resources. Herein, the adaptability of Toxopter...

  • Article
  • Open Access
5 Citations
3,255 Views
13 Pages

5 August 2023

Jamaica produces coffee marketed as Blue Mountain and high mountain (grown outside the Blue Mountains). Since the discovery of the coffee berry borer (CBB; Hypothenemus hampei) in Jamaica in 1978, chemical control has traditionally been the primary a...

  • Article
  • Open Access
7 Citations
2,351 Views
13 Pages

5 August 2023

The application of green manure is crucial for achieving sustainable agriculture and animal husbandry, but pest management is often overlooked. Conducting a risk assessment for insect pests in green manure is essential. The beet webworm, Loxostege st...

  • Article
  • Open Access
2 Citations
2,205 Views
15 Pages

4 August 2023

The Afrotropical hoverflies remain an understudied group of hoverflies. One of the reasons for the lack of studies on this group resides in the difficulties to delimit the species using the available identification keys. DNA barcoding has been found...

  • Article
  • Open Access
1 Citations
2,706 Views
18 Pages

Functional Divergence of the Tribolium castaneum engrailed and invected Paralogs

  • Summer Blunk,
  • Hector Garcia-Verdugo,
  • Sierra O’Sullivan,
  • James Camp,
  • Michael Haines,
  • Tara Coalter,
  • Terri A. Williams and
  • Lisa M. Nagy

4 August 2023

Engrailed (en) and invected (inv) encode paralogous transcription factors found as a closely linked tandem duplication within holometabolous insects. Drosophila en mutants segment normally, then fail to maintain their segments. Loss of Drosophil...

  • Review
  • Open Access
80 Citations
19,746 Views
16 Pages

4 August 2023

The relationship between insects and humans throughout history has always been complex and multifaceted. Insects are both a source of fascination and fear for humans and have played important roles in human culture, economy, and health. Nowadays, the...

  • Feature Paper
  • Article
  • Open Access
12 Citations
3,632 Views
18 Pages

Poor Air Quality Is Linked to Stress in Honeybees and Can Be Compounded by the Presence of Disease

  • Christopher Mayack,
  • Sarah E. Cook,
  • Bernardo D. Niño,
  • Laura Rivera,
  • Elina L. Niño and
  • Arathi Seshadri

4 August 2023

Climate change-related extreme weather events have manifested in the western United States as warmer and drier conditions with an increased risk of wildfires. Honeybees, essential for crop pollination in California, are at the center of these extreme...

  • Article
  • Open Access
11 Citations
3,305 Views
11 Pages

3 August 2023

In recent years, Aedes albopictus (Skuse, 1984) has expanded its distribution globally due to its high ecological plasticity. This expansion has increased the population’s susceptibility to contracting diseases such as dengue, Zika, and chikung...

  • Article
  • Open Access
6 Citations
2,781 Views
14 Pages

3 August 2023

Heortia vitessoides Moore, 1885 (Lepidoptera: Crambidae) is an economically important lepidopteran pest that caused severe damage to the plantation area of Aquilaria sinensis (Lour.) Gilg, 1825 (Thymelaeaceae), resulting in extensive defoliation of t...

  • Article
  • Open Access
5 Citations
2,547 Views
10 Pages

3 August 2023

Fall armyworm (FAW) is a major pest of maize and causes huge losses. Chemical pesticides are the commonly used control strategy among farmers. The efficacy of baculoviruses against FAW has been proven; however, farmers may not be able to afford the p...

  • Article
  • Open Access
4 Citations
2,673 Views
17 Pages

The Effects of Diet on the Immune Responses of the Oriental Armyworm Mythimna separata

  • Lizhen Zhou,
  • Li Ma,
  • Lu Liu,
  • Shaolei Sun,
  • Xiangfeng Jing and
  • Zhiqiang Lu

3 August 2023

Nutrients can greatly affect host immune defenses against infection. Possessing a simple immune system, insects have been widely used as models to address the relationships between nutrition and immunity. The effects of high versus low protein-to-car...

  • Article
  • Open Access
6 Citations
3,150 Views
15 Pages

Comprehensive Transcriptome Analysis in the Testis of the Silkworm, Bombyx mori

  • Kohei Kakino,
  • Hiroaki Mon,
  • Takeru Ebihara,
  • Masato Hino,
  • Akitsu Masuda,
  • Jae Man Lee and
  • Takahiro Kusakabe

2 August 2023

Spermatogenesis is an important process in reproduction and is conserved across species, but in Bombyx mori, it shows peculiarities, such as the maintenance of spermatogonia by apical cells and fertilization by dimorphic spermatozoa. In this study, w...

  • Article
  • Open Access
1 Citations
2,367 Views
16 Pages

An Optimized Small-Scale Rearing System to Support Embryonic Microinjection Protocols for Western Corn Rootworm, Diabrotica virgifera virgifera

  • Fu-Chyun Chu,
  • Pei-Shan Wu,
  • Sofia Pinzi,
  • Nathaniel Grubbs,
  • Allen Carson Cohen and
  • Marcé D. Lorenzen

2 August 2023

Western corn rootworm (WCR), a major pest of corn, has been reared in laboratories since the 1960s. While established rearing methods are appropriate for maintaining WCR colonies, they are not optimal for performing germline transformation or CRISPR/...

  • Article
  • Open Access
5 Citations
2,143 Views
13 Pages

Quarantine Disinfestation of Papaya Mealybug, Paracoccus marginatus (Hemiptera: Pseudococcidae) Using Gamma and X-rays Irradiation

  • Zi-Jiao Song,
  • Qing-Ying Zhao,
  • Chen Ma,
  • Ran-Ran Chen,
  • Tian-Bi Ma,
  • Zhi-Hong Li and
  • Guo-Ping Zhan

2 August 2023

Paracoccus marginatus is a highly polyphagous invasive pest that poses a significant quarantine threat to tropical and subtropical countries. Infested commodities in international trade should undergo phytosanitary treatment, and irradiation is...

  • Feature Paper
  • Article
  • Open Access
3 Citations
1,978 Views
20 Pages

Using Gamma Irradiation to Predict Sperm Competition Mechanism in Bagrada hilaris (Burmeister) (Hemiptera: Pentatomidae): Insights for a Future Management Strategy

  • Chiara Elvira Mainardi,
  • Chiara Peccerillo,
  • Alessandra Paolini,
  • Alessia Cemmi,
  • René F. H. Sforza,
  • Sergio Musmeci,
  • Daniele Porretta and
  • Massimo Cristofaro

1 August 2023

The stink bug, Bagrada hilaris, is a pest of mainly Brassicaceae crops. It is native to Africa and Asia and was recently reported as invasive in the southwestern part of the USA and in South America. There are no mitigation programs in place that do...

  • Article
  • Open Access
4 Citations
4,588 Views
19 Pages

Evaluation and Selection of New Trichogramma spp. as Biological Control Agents of the Guatemalan Potato Moth (Tecia solanivora) in Europe

  • Jorge Gavara,
  • Tomás Cabello,
  • Manuel Gámez,
  • Saskia Bastin,
  • Estrella Hernández-Suárez and
  • Ana Piedra-Buena

1 August 2023

The Guatemalan potato moth (Tecia solanivora) is designated a quarantine pest by the European Union, causing severe production losses in potato crops. No effective chemical control alternatives are currently available, and cultural techniques are una...

  • Article
  • Open Access
3 Citations
3,843 Views
26 Pages

31 July 2023

The assassin bug genus Argolis Stål, 1861 (Hemiptera: Reduviidae: Stenopodainae) has a disjunct distribution in Sub-Saharan Africa and Asia. In the present study, the Asian species of Argolis are revised. Two species are recognized, redescribed...

  • Article
  • Open Access
4 Citations
3,168 Views
20 Pages

31 July 2023

Megastigmidae comprises more than 200 species in 12 genera. Megastigmus has a worldwide distribution with more than 150 species. Over 80% of these species are recorded from the Australian and Palearctic region, with a few from Afrotropical and Orient...

  • Article
  • Open Access
3 Citations
2,785 Views
13 Pages

Comparison of Three Attractants for the Effective Capture of Xylotrechus chinensis Adults in Multi-Funnel Traps

  • Nickolas G. Kavallieratos,
  • Maria C. Boukouvala,
  • Anna Skourti,
  • Spyridon Antonatos,
  • Panos V. Petrakis,
  • Dimitrios P. Papachristos and
  • Georgios Th. Papadoulis

31 July 2023

The Asian coleopteran Xylotrechus chinensis (Chevrolat) (Cerambycidae: Cerambycinae) is an invasive species in several European countries, attacking mulberry trees. In the current research, we evaluated the performance of three mixtures consisting of...

  • Article
  • Open Access
4 Citations
2,543 Views
20 Pages

Mathematical Model for the Biological Control of the Coffee Berry Borer Hypothenemus hampei through Ant Predation

  • Carlos Andrés Trujillo-Salazar,
  • Gerard Olivar-Tost and
  • Deissy Milena Sotelo-Castelblanco

29 July 2023

Coffee is a relevant agricultural product in the global economy, with the amount and quality of the bean being seriously affected by the coffee berry borer Hypothenemus hampei (Ferrari), CBB, its principal pest. One of the ways to deal with this beet...

  • Article
  • Open Access
39 Citations
5,353 Views
19 Pages

Isolation and Identification of Antioxidant Peptides Derived from Cricket (Gryllus bimaculatus) Protein Fractions

  • Olumide Oluwatoyosi Fashakin,
  • Pipat Tangjaidee,
  • Kridsada Unban,
  • Wannaporn Klangpetch,
  • Tabkrich Khumsap,
  • Korawan Sringarm,
  • Saroat Rawdkuen and
  • Suphat Phongthai

29 July 2023

Crickets contain high protein content that can be used to improve nutrition but are less exploited. This study was conducted to isolate different Cricket Protein Fractions including albumin, globulin, glutelin, and prolamin. All fractions were charac...

  • Article
  • Open Access
1 Citations
4,057 Views
12 Pages

Tuta absoluta-Specific DNA in Domestic and Synanthropic Vertebrate Insectivore Feces

  • Dirk Janssen,
  • Emilio González-Miras and
  • Estefanía Rodríguez

28 July 2023

The ecology of greenhouse pests generally involves parasitoid or predatory insects. However, we investigated whether the leaf miner Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae) is part of the diet of domestic and synanthropic vertebrate a...

  • Article
  • Open Access
4 Citations
2,208 Views
11 Pages

28 July 2023

While herbivorous insect saliva plays a crucial role in the interaction between plants and insects, its role in the inter-specific interactions between herbivorous insects has received little attention. Pre-infestation of citrus plants with Aphis spi...

  • Feature Paper
  • Article
  • Open Access
13 Citations
2,597 Views
12 Pages

28 July 2023

The oriental fruit fly, Bactrocera dorsalis (Hendel), is a notorious invasive pest that has raised concerns worldwide. Validamycin has been demonstrated to be a very strong inhibitor against trehalase in a variety of organisms. However, whether valid...

  • Article
  • Open Access
1 Citations
2,613 Views
11 Pages

28 July 2023

Polarized light-based navigation in insects is facilitated by a polarization-sensitive part of the eye, the dorsal rim area (DRA). Existing methods to study the anatomy of the DRA are destructive and time-consuming. We presented a novel method for DR...

  • Article
  • Open Access
6 Citations
2,520 Views
13 Pages

27 July 2023

The gut bacterial microbiota of insects plays a crucial role in physiological, metabolic, and innate immune processes. In the current study, the gut bacterial communities of an insecticide-susceptible (IS), and a resistant (IR) population of a major...

  • Feature Paper
  • Article
  • Open Access
9 Citations
5,952 Views
17 Pages

26 July 2023

Apart from its economic value, industrial hemp (Cannabis sativa L.) is a prolific pollen producer, serving as a food source for bees. However, little is known regarding the extent to which varietal differences in hemp pollen chemistry influences bee...

  • Article
  • Open Access
2 Citations
2,570 Views
22 Pages

25 July 2023

Pseudaucalaspis pentagona and P. prunicola are notorious pests and commonly feed on various ornamental plants and fruit trees worldwide. The two species share many host-plant species, and are similar in morphological characteristics and life cycle, m...

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Insects - ISSN 2075-4450