Assessment of Phosphine Resistance in Major Stored-Product Insects in Greece Using Two Diagnostic Protocols
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Tested Species
2.2. Dose–Response Protocol
2.3. CORESTA Protocol
2.4. Data Analysis
3. Results
3.1. Dose–Response Protocol
3.2. CORESTA Protocol
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Population Code | Area Sampled | Commodity Sampled | Species Found |
---|---|---|---|
ASC3 | Sterea Hellas | Wheat flour | T. confusum |
ASC7 | Thrace | Wheat flour | S. oryzae |
ASC8 | Thrace | Wheat flour | S. granarius, C. ferrugineus |
ASC9 | Thessaly | Wheat flour | T. confusum |
ASC10 | Thessaly | Wheat flour | T. castaneum, O. surinamensis |
ASC11 | Peloponnese | Wheat | T. castaneum, R. dominica, O. surianemensis, S. oryzae |
ASC14 | Sterea Hellas | Cereals | T. confusum, R. dominica |
ASC15 | Peloponnese | Semolina | T. castaneum |
ASC16 | Peloponnese | Wheat flour | T. confusum |
ASC19 | Thessaly | Wheat flour | T. castaneum |
GA1 | Peloponnese | Βarley flour | S. oryzae, O. surinamensis |
GA2 | Macedonia | Wheat | R. dominica, C. ferrugineus, S. oryzae |
GA3 | Thessaly | Wheat | R. dominica |
GA6 | Thessaly | Wheat | R. dominica |
GA12 | Thessaly | Wheat | T. confusum, R. dominica |
U1 | Macedonia | Wheat | S. oryzae |
V1 | Crete | Wheat flour | T. confusum |
V2 | Crete | Wheat flour | T. confusum |
P1 | Crete | Bran flour | T. confusum |
PP1 | Crete | Residues | T. confusum |
P2(1) | Crete | Residues | T. confusum |
P2(2) | Crete | Residues | T. confusum |
P3 | Crete | Residues | T. confusum |
P4 | Crete | Residues | T. confusum |
F1 | Crete | Residues | T. confusum |
F2 | Crete | Residues | T. confusum |
F3 | Crete | Residues | T. confusum |
F4 | Crete | Residues | T. confusum |
F5 | Crete | Residues | T. confusum |
S1 | Crete | Residues | T. confusum |
S2 | Crete | Residues | T. confusum |
S3 | Crete | Residues | T. confusum |
SK1 | Crete | Residues | T. confusum |
SK2 | Crete | Residues | T. confusum |
SKG1 | Crete | Residues | T. confusum |
SKG2 | Crete | Residues | T. confusum |
AGR-01 | Sterea Hellas | Grain byproducts | S. oryzae, R. dominica, T. confusum |
AGR-03 | Sterea Hellas | Rice | R. dominica |
AGR-04 | Sterea Hellas | Barley flour | T. castaneum |
AGR-05 | Sterea Hellas | Wheat flour | T. confusum |
EXT1 | Macedonia | Rice | O. surinamensis |
LAB | University of Thessaly | Preferred rearing media | S. oryzae, S. granarius, O. surinamensis, T. confusum, T. castaneum, R. dominica, C. ferrugineus |
Species | Populations | LC50 (Confidence Interval) | LC99 (Confidence Interval) | Y-Intercept | x2 | p | Diagnosis a |
---|---|---|---|---|---|---|---|
S. granarius | ASC8 | ** | ** | ** | ** | ** | Susceptible |
LAB | ** | ** | ** | ** | ** | Susceptible | |
O. surinamensis | ASC10 | ** | ** | ** | ** | ** | Susceptible |
ASC11 | 102.5 * | 557.8 * | −6.35 | 400.0 | <0.01 | Resistant | |
GA1 | 72.9 (46–107) | 152.1 (104.8–1049) | −13.58 | 954.0 | <0.01 | Resistant | |
EXTR1 | ** | ** | ** | ** | ** | Susceptible | |
LAB | ** | ** | ** | ** | ** | Susceptible | |
S. oryzae | GA2 | ** | ** | ** | ** | ** | Susceptible |
GA1 | 0.7 * | 27.6 * | 0.16 | 13.8 | 0.99 | Susceptible | |
ASC7 | ** | ** | ** | ** | ** | Susceptible | |
ASC11 | 43 (26.7–54.3) | 231.6 (156.8–585.3) | −5.19 | 124.9 | <0.01 | Resistant | |
U1 | 7.0 * | 58.8 * | −2.15 | 13.1 | 1.00 | Susceptible | |
AGR-01 | ** | ** | ** | ** | ** | Susceptible | |
LAB | ** | ** | ** | ** | ** | Susceptible | |
T. confusum | ASC3 | 12.2 * | 73 * | −3.27 | 50.0 | 0.03 | Resistant |
ASC9 | ** | ** | ** | ** | ** | Susceptible | |
ASC14 | 20.1 (3–30) | 86.5 (69.3–214) | −4.79 | 30.5 | 0.63 | Resistant | |
V1 | 17.2 * | 136.1 * | −3.21 | 102.5 | <0.01 | Resistant | |
V2 | 13.9 * | 75 * | −3.63 | 18.8 | 0.98 | Resistant | |
P1 | 19.2 * | 85.1 * | −4.63 | 24.6 | 0.88 | Resistant | |
AGR-01 | ** | ** | ** | ** | ** | Susceptible | |
AGR-05 | ** | ** | ** | ** | ** | Susceptible | |
ASC16 | 0.8 | 27.6 * | 0.16 | 13.8 | 0.99 | Susceptible | |
GA12 | 43.0 * | 177.2 * | −6.17 | 123.1 | <0.01 | Resistant | |
F1 | ** | ** | ** | ** | ** | Susceptible | |
F2 | ** | ** | ** | ** | ** | Susceptible | |
F3 | ** | ** | ** | ** | ** | Susceptible | |
F4 | 0.7 | 27.6 | 0.16 | 14.0 | 0.99 | Susceptible | |
F5 | ** | ** | ** | ** | ** | Susceptible | |
PP1 | ** | ** | ** | ** | ** | Susceptible | |
P2(1) | ** | ** | ** | ** | ** | Susceptible | |
P2(2) | ** | ** | ** | ** | ** | Susceptible | |
P3 | ** | ** | ** | ** | ** | Susceptible | |
P4 | ** | ** | ** | ** | ** | Susceptible | |
S1 | 0.7 | 27.6 | 0.16 | 13.7 | 0.99 | Susceptible | |
S2 | ** | ** | ** | ** | ** | Susceptible | |
S3 | ** | ** | ** | ** | ** | Susceptible | |
SKG1 | 9.0 | 64.6 | −2.61 | 15.1 | 0.99 | Resistant | |
SKG2 | ** | ** | ** | ** | ** | Susceptible | |
SK1 | ** | ** | ** | ** | ** | Susceptible | |
SK2 | ** | ** | ** | ** | ** | Susceptible | |
LAB | ** | ** | ** | ** | ** | Susceptible | |
C. ferrugineus | ASC8 | 1.8 * | 42.3 * | −0.45 | 25.8 | 0.84 | Susceptible |
GA2 | 16.5 * | 375.6 * | −2.09 | 56.3 | 0.01 | Resistant | |
LAB | ** | ** | ** | ** | ** | Susceptible | |
T. castaneum | ASC10 | ** | ** | ** | ** | ** | Susceptible |
ASC11 | 63.0 * | 290.4 * | −6.31 | 119.6 | <0.01 | Resistant | |
ASC15 | ** | ** | ** | ** | ** | Susceptible | |
ASC19 | ** | ** | ** | ** | ** | Susceptible | |
AGR-04 | ** | ** | ** | ** | ** | Susceptible | |
LAB | ** | ** | ** | ** | ** | Susceptible | |
R. dominica | ASC11 | 219.3 * | 1766.4 * | −6.02 | 93.0 | <0.01 | Resistant |
GA6 | 297.9 * | 1134.6 * | −9.91 | 317.9 | <0.01 | Resistant | |
GA3 | 308.6 * | 1541 * | −8.29 | 236.2 | <0.01 | Resistant | |
GA2 | 18.8 * | 141.7 * | −3.38 | 45.8 | 0.08 | Resistant | |
AGR-03 | 264 * | 1296.9 * | −8.14 | 101.7 | <0.01 | Resistant | |
GA12 | 156.7 * | 309.5 * | −17.27 | 2191 | <0.01 | Resistant | |
ASC14 | 281.6 * | 1668.3 * | −7.37 | 150.0 | <0.01 | Resistant | |
AGR-01 | 23.0 * | 303 * | −2.83 | 157.9 | <0.01 | Resistant | |
LAB | ** | ** | ** | ** | ** | Susceptible |
Species | Population | 50 | 100 | 200 | 1000 | F | p |
---|---|---|---|---|---|---|---|
S. granarius | ASC8 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | ||
LAB | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
O. surinamensis | ASC10 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | ||
ASC11 | 4.4 ± 1.9 A | 28.3 ± 14.0 A | 22.2 ± 12.1 A | 100.0 ± 0.0 B | 20.368 | <0.001 | |
GA1 | 6.6 ± 2.5 A | 70.0 ± 10.1 B | 41.1 ± 12.2 C | 100.0 ± 0.0 B D | 24.542 | <0.001 | |
EXTR1 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
LAB | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
S. oryzae | GA2 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | ||
GA1 | 99.5 ± 0.5 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 1.00 | 0.405 | |
ASC7 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
ASC11 | 56.5 ± 11.02 A | 63.9 ± 4.3 AB | 81.6 ± 3.8 BC | 100.0 ± 0.0 CD | 9.720 | <0.001 | |
U1 | 96.5 ± 2.7 | 99.4 ± 0.6 | 100.0 ± 0.0 | 100.0 ± 0.0 | 1.282 | 0.297 | |
AGR-01 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
LAB | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
T. confusum | ASC3 | 92.2 ± 7.7 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 1.00 | 0.405 |
ASC9 | 100.0 ± 0.0 | 99.4 ± 0.6 | 100.0 ± 0.0 | 100.0 ± 0.0 | 1.00 | 0.405 | |
ASC14 | 80.0 ± 5.9 A | 88.9 ± 2.0 AB | 100.0 ± 0.0 B | 100.0 ± 0.0 B | 9.689 | <0.001 | |
V1 | 68.3 ± 10.3 A | 93.9 ± 3.7 B | 96.1 ± 1.6 B | 100.0 ± 0.0 B | 6.680 | <0.001 | |
V2 | 80.6 ± 7.7 A | 95.0 ± 2.0 AB | 97.7 ± 1.6 B | 100.0 ± 0.0 B | 4.532 | 0.009 | |
P1 | 75.5 ± 2.5 A | 92.2 ± 3.9 B | 96.1 ± 1.8 B | 100.0 ± 0.0 B | 18.245 | <0.001 | |
AGR-01 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
AGR-05 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
ASC16 | 99.4 ± 0.6 | 100.0 ± 0.0 | 99.4 ± 0.6 | 100.0 ± 0.0 | 0.667 | 0.579 | |
GA12 | 28.9 ± 14.5 A | 50.0 ± 12.6 BC | 80.0 ± 4.7 AB | 100.0 ± 0.0 C | 10.055 | <0.001 | |
F1 | 93.3 ± 1.8 A | 87.2 ± 2.5 B | 100.0 ± 0.0 C | 100.0 ± 0.0 C | 15.402 | <0.001 | |
F2 | 83.9 ± 4.2 A | 92.7 ± 3.9 AB | 99.4 ± 0.6 B | 99.4 0.6 B | 6.286 | 0.002 | |
F3 | 91.1 ± 2.9 A | 94.4 ± 2.9 AB | 99.4 ± 0.6 B | 100.0 ± 0.0 B | 4.035 | 0.015 | |
F4 | 88.9 ± 2.8 | 92.2 ± 3.2 | 99.4 ±0.6 | 100.0 ± 0.0 | 6.304 | 0.002 | |
F5 | 82.7 ± 2.3 A | 91.6 ± 2.8 B | 100.0 ± 0.0 C | 100.0 ± 0.0 C | 19.359 | <0.001 | |
PP1 | 85.6 ± 2.1 | 92.7 ± 2.6 | 100.0 ± 0.0 | 100.0 ± 0.0 | 16.635 | <0.001 | |
P2(1) | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
P2(2) | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
P3 | 99.4 ± 0.6 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 1.00 | 0.405 | |
P4 | 99.4 ± 0.6 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 1.00 | 0.405 | |
S1 | 71.6 ± 4.4 A | 86.6 ± 4.1 B | 100.0 ± 0.0 C | 100.0 ± 0.0 C | 19.799 | <0.001 | |
S2 | 85.6 ± 4.1 C | 89.4 ± 2.8 BC | 97.2 ± 1.4 AB | 100.0 ± 0.0 A | 6.640 | 0.001 | |
S3 | 89.4 ± 1.3 A | 92.2 ±1.8 A | 98.3 ± 1.1 B | 100.0 ± 0.0 B | 15.008 | <0.001 | |
SKG1 | 77.2 ± 2.7 A | 95.0 ±5.0 B | 100.0 ± 0.0 B | 100.0 ± 0.0 B | 14.302 | <0.001 | |
SKG2 | 87.2 ± 2.6 A | 91.1 ± 3.2 A | 99.4 ±0.6 B | 100.0 ± 0.0 B | 9.029 | <0.001 | |
SK1 | 73.3 ± 2.8 A | 100.0 ± 0.0 B | 100.0 ± 0.0 B | 100.0 ± 0.0 B | 85.333 | <0.001 | |
SK2 | 85.0 ± 2.6 A | 89.4 ±2.1 A | 100.0 ± 0.0 B | 100.0 ± 0.0 B | 20.216 | <0.001 | |
LAB | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
C. ferrugineus | ASC8 | 98.9 ± 1.1 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ±0.0 | 1.000 | 0.405 |
GA2 | 78.9 ± 5.9 A | 80.6 ± 4.3 A | 88.3 ± 2.0 AB | 100.0 ± 0.0 B | 7.098 | 0.001 | |
LAB | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
T. castaneum | ASC10 | 99.4 ± 0.6 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 1.000 | 0.405 |
ASC11 | 6.6 ± 2.3 A | 10.6 ± 5.9 A | 7.2 ± 0.3 A | 100.0 ± 0.0 B | 91.093 | <0.001 | |
ASC15 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
ASC19 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
AGR-04 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
LAB | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
R. dominica | ASC11 | 7.7 ± 3.1 A | 24.4 ± 15.3 B | 28.9 ± 4.2 B | 100.0 ± 0.0 C | 154.115 | <0.001 |
GA6 | 2.2 ± 0.8 A | 1.1 ± 1.1 A | 20.0 ±1.1 A | 100.0 ± 0.0 B | 66.768 | <0.001 | |
GA3 | 1.6 ± 0.8 A | 5.6 ± 1.5 A | 16.1 ± 4.7 B | 100.0 ± 0.0 C | 334.790 | <0.001 | |
GA2 | 84.4 ±5.6 A | 100.0 ± 0.0 B | 100.0 ± 0.0 B | 100.0 ±0.0 B | 7.840 | <0.001 | |
AGR-03 | 3.3 ± 1.1 A | 5.0 ± 1.6 A | 26.1 ±7.9 B | 100.0 ± 0.0 C | 122.985 | <0.001 | |
GA12 | 1.1 ± 0.7 A | 1.1 ± 0.7 A | 79.4 ± 8.1 B | 100.0 ± 0.0 C | 157.973 | <0.001 | |
ASC14 | 6.6 ± 2.2 A | 6.1 ± 1.3 A | 20.0 ± 5.3 B | 100.0 ± 0.0 C | 229.681 | <0.001 | |
AGR-01 | 35.0 ± 7.4 A | 88.3 ± 4.7 B | 100.0 ± 0.0 B | 100.0 ± 0.0 B | 49.571 | <0.001 | |
LAB | 100.0 ± 0.0 | 100.0 ±0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 |
Species | Population | LC50 (Confidence Interval) | LC99 (Confidence Interval) | Y-Intercept | x2 | p | Diagnosis a |
---|---|---|---|---|---|---|---|
S. granarius | ASC8 | ** | ** | ** | ** | ** | Susceptible |
LAB | ** | ** | ** | ** | ** | Susceptible | |
O. surinamensis | ASC10 | ** | ** | ** | ** | ** | Susceptible |
ASC11 | 100 * | 622 * | −5.69 | 419.3 | <0.01 | Resistant | |
GA1 | 71.6 * | 176.5 * | −11.02 | 243.7 | <0.01 | Resistant | |
EXTR1 | ** | ** | ** | ** | ** | Susceptible | |
LAB | ** | ** | ** | ** | ** | Susceptible | |
S. oryzae | GA2 | 0.7 * | 27.6 * | 0.16 | 13.8 | 0.99 | Susceptible |
GA1 | 0.7 * | 27.6 * | 0.16 | 13.7 | 0.99 | Susceptible | |
ASC7 | ** | ** | ** | ** | ** | Susceptible | |
ASC11 | 34.2 (16–45) | 176.1 (119.5–560) | −5.02 | 113.0 | <0.01 | Resistant | |
U1 | 0.7 * | 27.6 * | 0.16 | 13.7 | 0.99 | Susceptible | |
AGR-01 | ** | ** | ** | ** | ** | Susceptible | |
LAB | ** | ** | ** | ** | ** | Susceptible | |
T. confusum | ASC3 | ** | ** | ** | ** | ** | Susceptible |
ASC9 | ** | ** | ** | ** | ** | Susceptible | |
ASC14 | 17.8 (6.3–27) | 141.7 (106.4–277) | −3.23 | 31.3 | 0.54 | Resistant | |
V1 | 24.9 (6.6–36.9) | 157.3 (107.7–557.3) | −4.06 | 88.9 | <0.01 | Resistant | |
V2 | 20.8 (0.4–32.8) | 87.9 (67.8–615.1) | −4.9 | 50.8 | 0.03 | Resistant | |
P1 | 18.3 (6.7–27.4) | 139.9 (105–276) | −3.33 | 17.3 | 0.99 | Resistant | |
AGR-01 | ** | ** | ** | ** | ** | Susceptible | |
AGR-05 | ** | ** | ** | ** | ** | Susceptible | |
ASC16 | 0.7 * | 27.6 * | 0.16 | 13.8 | 0.99 | Susceptible | |
GA12 | 47.5 (29.8–58.8) | 172.7 (118–549.2) | −6.93 | 200 | <0.01 | Resistant | |
F1 | 0.7 * | 27.6 * | 0.16 | 13.8 | 0.99 | Susceptible | |
F2 | 0.3 * | 79.3 * | 0.46 | 60.3 | <0.01 | Susceptible | |
F3 | 1.8 * | 42.3 * | −0.45 | 25.8 | 0.84 | Susceptible | |
F4 | 9.0 * | 64.6 * | −2.61 | 15.1 | 0.99 | Resistant | |
F5 | 0.9 * | 31.8 * | 0.94 | 24.6 | 0.88 | Susceptible | |
PP1 | 0.7 * | 27.6 * | 0.16 | 13.7 | 0.99 | Susceptible | |
P2(1) | ** | ** | ** | ** | ** | Susceptible | |
P2(2) | ** | ** | ** | ** | ** | Susceptible | |
P3 | ** | ** | ** | ** | ** | Susceptible | |
P4 | ** | ** | ** | ** | ** | Susceptible | |
S1 | 9.7 (0.3–21.4) | 167.1 (110–763) | −1.86 | 44.4 | 0.10 | Resistant | |
S2 | ** | ** | ** | ** | ** | Susceptible | |
S3 | 13.9 (0–27.1) | 75 (59.2–1027) | −3.63 | 10.7 | 0.99 | Resistant | |
SKG1 | 0.7 * | 27.6 * | 0.16 | 13.7 | 0.99 | Susceptible | |
SKG2 | 10.9 * | 68.4 * | −3.02 | 19.9 | 0.97 | Resistant | |
SK1 | 19.2 * | 85.1 * | −4.63 | 13.6 | 0.99 | Resistant | |
SK2 | 7.0 * | 58.8 * | −2.15 | 25.9 | 0.83 | Resistant | |
LAB | ** | ** | ** | ** | ** | Susceptible | |
C. ferrugineus | ASC8 | 1.8 * | 42.3 * | −0.45 | 25.8 | 0.84 | Susceptible |
GA2 | 5.0 * | 1017 * | −0.71 | 58.8 | <0.01 | Resistant | |
LAB | ** | ** | ** | ** | ** | Susceptible | |
T. castaneum | ASC10 | ** | ** | ** | ** | ** | Susceptible |
ASC11 | 82.6 * | 313 * | −7.71 | 87.4 | <0.01 | Resistant | |
ASC15 | ** | ** | ** | ** | ** | Susceptible | |
ASC19 | ** | ** | ** | ** | ** | Susceptible | |
AGR-04 | ** | ** | ** | ** | ** | Susceptible | |
LAB | ** | ** | ** | ** | ** | Susceptible | |
R. dominica | ASC11 | 146 * | 2758 * | −3.95 | 114.7 | <0.01 | Resistant |
GA6 | 193.2 * | 1517 * | −5.93 | 89.8 | <0.01 | Resistant | |
GA3 | 173.5 * | 1882 * | −5.03 | 85.5 | <0.01 | Resistant | |
GA2 | 22.3 * | 90.1 * | −5.18 | 32.7 | 0.30 | Resistant | |
AGR-03 | 139.9 * | 888.8 * | −6.21 | 55.2 | <0.01 | Resistant | |
GA12 | 123.6 * | 293.5 * | −12.96 | 289.8 | <0.01 | Resistant | |
ASC14 | 209.1 * | 1607 * | −6.09 | 66.5 | <0.01 | Resistant | |
AGR-01 | ** | ** | ** | ** | ** | Susceptible | |
LAB | ** | ** | ** | ** | ** | Susceptible |
Species | Population | 50 | 100 | 200 | 1000 | F | p |
---|---|---|---|---|---|---|---|
S. granarius | ASC8 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | ||
LAB | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
O. surinamensis | ASC10 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | ||
ASC11 | 11.6 ± 4.1 | 37.2 ± 15.9 | 96.6 ± 2.2 | 100.0 ± 0.0 | 28.002 | <0.001 | |
GA1 | 10.0 ± 3.8 | 93.9 ± 3.3 | 94.4 ±2.8 | 100.0 ± 0.0 | |||
EXTR1 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
LAB | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
S. oryzae | GA2 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | ||
GA1 | 99.4 ± 0.6 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 1.000 | 0.405 | |
ASC7 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
ASC11 | 65.0 ± 11.8 A | 100.0 ± 0.0 B | 100.0 ± 0.0 B | 100.0 ± 0.0 B | 8.733 | <0.001 | |
U1 | 99.4 ± 0.6 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 1.000 | 0.405 | |
AGR-01 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
LAB | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
T. confusum | ASC3 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | ||
ASC9 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
ASC14 | 85 ± 4.4 | 98.3 ± 1.1 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
V1 | 77.7 ± 8.9 | 98.9 ± 0.7 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
V2 | 90.6 ± 6.0 | 99.4 ± 0.6 | 98.9 ± 0.7 | 100.0 ± 0.0 | 2.103 | 0.119 | |
P1 | 85.0 ± 0.0 A | 99.4 ± 0.6 B | 98.9 ± 1.1 B | 100.0 ± 0.0 B | 21.208 | <0.001 | |
AGR-01 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
AGR-05 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
ASC16 | 99.4 ± 0.6 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 1.000 | 0.405 | |
GA12 | 41.1 ± 15.5 A | 81.1 ± 6.7 B | 100.0 ± 0.0 B | 100.0 ± 0.0 B | 10.710 | <0.001 | |
F1 | 97.2 ± 1.2 | 97.2 ± 1.2 | 100.0 ± 0.0 | 100.0 ± 0.0 | 3.509 | 0.026 | |
F2 | 92.7 ± 1.6 A | 95.0 ± 1.6 AB | 100.0 ± 0.0 B | 100.0 ± 0.0 B | 4.468 | 0.010 | |
F3 | 93.3 ± 2.8 | 97.2 ± 1.6 | 100.0 ± 0.0 | 100.0 ± 0.0 | 3.559 | 0.025 | |
F4 | 91.6 ± 2.2 | 98.9 ± 1.1 | 100.0 ± 0.0 | 100.0 ± 0.0 | 10.582 | <0.001 | |
F5 | 94.4 ± 1.5 | 96.1 ± 1.6 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
PP1 | 92.7 ± 1.4 A | 97.7 ± 1.2 B | 100.0 ± 0.0 B | 100.0 ± 0.0 B | 12.794 | <0.001 | |
P2(1) | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
P2(2) | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
P3 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
P4 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
S1 | 83.3 ± 3.4 A | 96.6 ± 2.7 B | 100.0 ± 0.0 B | 100.0 ± 0.0 B | 12.952 | <0.001 | |
S2 | 96.1 ± 1.1 | 96.1 ± 1.6 | 99.4 ± 0.6 | 100.0 ± 0.0 | 4.222 | 0.013 | |
S3 | 91.6 ± 1.6 A | 97.2 ± 1.2 B | 99.4 ± 0.6 B | 100.0 ± 0.0 B | 12.723 | <0.001 | |
SKG1 | 88.9 ± 2.1 A | 100.0 ± 0.0 B | 100.0 ± 0.0 B | 100.0 ± 0.0 B | 26.230 | <0.001 | |
SKG2 | 90.0 ± 2.2 A | 98.9 ± 0.7 B | 99.2 ±0.6 B | 100.0 ± 0.0 B | 12.766 | <0.001 | |
SK1 | 86.1 ± 1.8 A | 100.0 ± 0.0 B | 100.0 ± 0.0 B | 100.0 ± 0.0 B | 58.140 | <0.001 | |
SK2 | 93.3 ± 1.6 A | 98.9 ± 0.7 B | 100.0 ± 0.0 B | 100.0 ± 0.0 B | 12.279 | <0.001 | |
LAB | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
C. ferrugineus | ASC8 | 98.9 ± 1.1 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 1.000 | 0.405 |
GA2 | 88.4 ± 4.6 A | 84.4 ± 2.9 A | 93.9 ± 1.3 AB | 100.0 ± 0.0 B | 5.718 | 0.003 | |
LAB | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
T. castaneum | ASC10 | 99.4 ± 0.6 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 1.000 | 0.405 |
ASC11 | 18.3 ± 4.8 A | 50.0 ±6.8 B | 93.9 ± 2.8 C | 100.0 ± 0.0 C | 82.535 | <0.001 | |
ASC15 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
ASC19 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
AGR-04 | 98.3 ± 1.1 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 2.000 | 0.134 | |
LAB | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
R. dominica | ASC11 | 32.7 ± 5.8 A | 35.0 ± 5.0 A | 45.0 ± 4.2 A | 100.0 ± 0.0 B | 51.940 | <0.001 |
GA6 | 15.6 ± 2.9 A | 14.4 ± 2.8 A | 46.1 ± 7.5 B | 100.0 ± 0.0 C | 87.637 | <0.001 | |
GA3 | 20.6 ± 3.3 A | 26.6 ± 3.2 AB | 43.9 ± 8.1 B | 100.0 ± 0.0 C | 59.765 | <0.001 | |
GA2 | 90.6 ± 4.2 A | 100.0 ± 0.0 B | 100.0 ± 0.0 B | 100.0 ± 0.0 B | 5.048 | 0.006 | |
AGR-03 | 15.6 ± 2.6 A | 21.1 ± 1.1 A | 71.1 ± 5.6 B | 100.0 ± 0.0 C | 161.135 | <0.001 | |
GA12 | 6.6 ± 2.3 A | 6.1 ± 2.0 A | 98.8 ± 0.7 B | 100.0 ± 0.0 B | 1142.341 | <0.001 | |
ASC14 | 11.6 ± 1.6 A | 17.7 ± 3.0 A | 37.7 ± 5.7 B | 100.0 ± 0.0 C | 143.907 | <0.001 | |
AGR-01 | 100.0 ± 0.0 | 100.0 ±0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
LAB | 100.0 ± 0.0 | 100.0 ±0.0 | 100.0 ± 0.0 | 100.0 ± 0.0 |
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Agrafioti, P.; Kaloudis, E.; Kateris, D.; Athanassiou, C.G. Assessment of Phosphine Resistance in Major Stored-Product Insects in Greece Using Two Diagnostic Protocols. Insects 2024, 15, 802. https://doi.org/10.3390/insects15100802
Agrafioti P, Kaloudis E, Kateris D, Athanassiou CG. Assessment of Phosphine Resistance in Major Stored-Product Insects in Greece Using Two Diagnostic Protocols. Insects. 2024; 15(10):802. https://doi.org/10.3390/insects15100802
Chicago/Turabian StyleAgrafioti, Paraskevi, Efstathios Kaloudis, Dimitrios Kateris, and Christos G. Athanassiou. 2024. "Assessment of Phosphine Resistance in Major Stored-Product Insects in Greece Using Two Diagnostic Protocols" Insects 15, no. 10: 802. https://doi.org/10.3390/insects15100802