Endosymbiotic Fungal Diversity and Dynamics of the Brown Planthopper across Developmental Stages, Tissues, and Sexes Revealed Using Circular Consensus Sequencing
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Insects
2.2. Sample Collection
2.3. BPH Virulence Identification
2.4. DNA Extraction and PCR Amplification
2.5. Library Construction and Sequencing
2.6. Statistical and Bioinformatics Analysis
3. Results
3.1. General Profile of ITS Amplicon Sequencing
3.2. The Composition of Fungal Communities
3.3. Fungal Community Structure Comparisons
3.4. Comparison of Species Composition and Diversity between Female and Male Adults in Different Tissues
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Sample | Average Length (bp) 1 | OTUs | Chao1 1 | ACE 1 | Shannon 1 | Simpson 1 |
---|---|---|---|---|---|---|
D0 | 697.18 ± 64.70 | 8910 | 2438.46 ± 301.77 | 2685.45 ± 392.33 | 6.02 ± 0.49 | 0.97 ± 0.01 |
D1 | 679.50 ± 7.40 | 7670 | 4211.15 ± 1128.26 | 4473.42 ± 1181.89 | 5.26 ± 0.42 | 0.91 ± 0.03 |
D2 | 682.52 ± 5.24 | 7376 | 5050.25 ± 436.99 | 5387.22 ± 405.28 | 5.32 ± 0.13 | 0.91 ± 0.02 |
D3 | 682.24 ± 3.11 | 7920 | 5694.85 ± 339.04 | 5991.18 ± 356.93 | 5.41 ± 0.08 | 0.91 ± 0.00 |
D4 | 722.13 ± 19.83 | 8548 | 5258.31 ± 1571.99 | 5566.48 ± 1568.56 | 5.50 ± 0.20 | 0.91 ± 0.01 |
D5 | 702.84 ± 7.32 | 7150 | 5328.88 ± 428.94 | 5653.63 ± 434.55 | 5.35 ± 0.02 | 0.90 ± 0.00 |
DF | 694.77 ± 1.96 | 7560 | 4117.70 ± 1121.43 | 4410.26 ± 1053.31 | 5.64 ± 0.41 | 0.92 ± 0.02 |
DM | 666.93 ± 11.08 | 7660 | 5119.20 ± 155.58 | 5641.36 ± 217.72 | 5.75 ± 0.06 | 0.96 ± 0.00 |
F-Sal | 612.42 ± 12.27 | 7692 | 1967.43 ± 209.16 | 2116.07 ± 193.72 | 5.39 ± 0.70 | 0.92 ± 0.04 |
F-Mid | 677.73 ± 59.68 | 7078 | 2003.80 ± 221.89 | 2162.75 ± 196.80 | 5.50 ± 0.74 | 0.92 ± 0.06 |
F-Mal | 615.61 ± 14.93 | 4934 | 2058.24 ± 539.83 | 2208.23 ± 348.79 | 4.62 ± 0.23 | 0.88 ± 0.07 |
F-Hin | 658.89 ± 66.65 | 6402 | 2098.50 ± 342.34 | 2247.86 ± 261.80 | 4.60 ± 0.41 | 0.83 ± 0.03 |
F-Ova | 732.75 ± 82.09 | 5887 | 2511.27 ± 731.67 | 2660.79 ± 643.26 | 4.83 ± 0.31 | 0.89 ± 0.04 |
F-Fat | 676.28 ± 7.26 | 7951 | 2903.54 ± 348.07 | 3091.26 ± 334.51 | 5.48 ± 0.64 | 0.93 ± 0.03 |
F-Hon | 628.93 ± 56.37 | 4818 | 1378.55 ± 241.18 | 1573.77 ± 310.57 | 5.01 ± 0.15 | 0.96 ± 0.01 |
M-Sal | 620.45 ± 17.57 | 5581 | 2709.21 ± 508.60 | 2987.41 ± 522.73 | 4.94 ± 0.18 | 0.92 ± 0.02 |
M-Mid | 617.28 ± 4.61 | 7056 | 2787.83 ± 611.12 | 3046.64 ± 703.01 | 5.28 ± 0.58 | 0.93 ± 0.02 |
M-Mal | 632.90 ± 17.83 | 8825 | 2685.96 ± 610.87 | 2946.71 ± 711.27 | 5.89 ± 0.59 | 0.96 ± 0.01 |
M-Hin | 689.25 ± 74.71 | 8349 | 2437.76 ± 455.96 | 2857.79 ± 596.39 | 5.26 ± 0.35 | 0.96 ± 0.03 |
M-Tes | 704.38 ± 25.59 | 8010 | 3668.89 ± 703.50 | 4079.21 ± 826.86 | 5.43 ± 0.18 | 0.95 ± 0.02 |
M-Fat | 684.36 ± 7.27 | 7842 | 3399.84 ± 639.60 | 3697.81 ± 777.00 | 5.15 ± 0.54 | 0.90 ± 0.03 |
All | 693.78 ± 94.97 | 87,131 | 3337.92 ± 1455.67 | 3608.31 ± 1524.12 | 5.31 ± 0.73 | 0.92 ± 0.10 |
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Cheng, Y.; Yang, J.; Li, T.; Li, J.; Ye, M.; Wang, J.; Chen, R.; Zhu, L.; Du, B.; He, G. Endosymbiotic Fungal Diversity and Dynamics of the Brown Planthopper across Developmental Stages, Tissues, and Sexes Revealed Using Circular Consensus Sequencing. Insects 2024, 15, 87. https://doi.org/10.3390/insects15020087
Cheng Y, Yang J, Li T, Li J, Ye M, Wang J, Chen R, Zhu L, Du B, He G. Endosymbiotic Fungal Diversity and Dynamics of the Brown Planthopper across Developmental Stages, Tissues, and Sexes Revealed Using Circular Consensus Sequencing. Insects. 2024; 15(2):87. https://doi.org/10.3390/insects15020087
Chicago/Turabian StyleCheng, Yichen, Jing Yang, Tianzhu Li, Jiamei Li, Meng Ye, Jing Wang, Rongzhi Chen, Lili Zhu, Bo Du, and Guangcun He. 2024. "Endosymbiotic Fungal Diversity and Dynamics of the Brown Planthopper across Developmental Stages, Tissues, and Sexes Revealed Using Circular Consensus Sequencing" Insects 15, no. 2: 87. https://doi.org/10.3390/insects15020087