Transcriptomic Profiling of Bean Aphid Megoura crassicauda upon Exposure to the Aphid-Obligate Entomopathogen Conidiobolus obscurus (Entomophthoromycotina) and Screening of CytCo-Binding Aphid Proteins through a Pull-Down Assay
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Fungus Origin and Preparation
2.2. Aphid Inoculation Procedure
2.3. Sampling of Aphids and RNA Extraction
2.4. Transcriptome Analysis
2.5. The Real-Time Quantitative PCR (qPCR) Assay
2.6. Pull-Down Assay
2.7. Identification of the Potential CytCo-Binding Proteins by LC-MS/MS
3. Results
3.1. Transcriptome Assembly and Annotation
3.2. Functional Enrichment Analysis of Differentially Expressed Genes
3.3. Identification of CytCo-Binding Proteins
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Unigene ID | Annotation | FPKM | Log2(FC) b | GO/KO ID | |
---|---|---|---|---|---|
Healthy | Inoculated | ||||
Immunoglobulin | |||||
c57868 | Immunoglobulin I-set domain | 1.81 | 6.99 | 1.70 | K23915 |
c56461 | Immunoglobulin V-set domain | 0.39 | 1.84 | 1.48 | K06772 |
c58593 | Immunoglobulin V-set domain | 1.33 | 3.66 | 1.23 | GO:0016021 |
c56079 | Immunoglobulin I-set domain | 2.33 | 5.85 | 1.17 | GO:0016021 |
c54209 | Immunoglobulin V-set domain | 0.84 | 2.25 | 1.13 | K22655 |
c56508 | Immunoglobulin I-set domain | 1.06 | 2.64 | 1.01 | GO:0016021 |
Peroxidase or ROS-related | |||||
c55353 * | Chorion peroxidase | 7.95 | 4.12 | −1.01 | K19511 |
c59130 | Animal haem peroxidase | 245.04 | 120.77 | −1.04 | K19511 |
c59021 | Melanization protease | 125.49 | 56.73 | −1.27 | GO:0004252 |
c55437 | Phenoloxidase-activating enzyme | 120.31 | 35.41 | −1.34 | GO:0004252 |
c58805 | Glutamate transporter polyphemus | 25.40 | 7.20 | −1.58 | K14209 |
Wound healing | |||||
c54775 | Fibronectin type III | 5.48 | 18.32 | 1.78 | K17591 |
c51444 | Mucin 2-like protein | 0.23 | 1.08 | 1.17 | / |
c54290 | Fibronectin type III | 2.55 | 6.57 | 1.11 | GO:0005515 |
c57232 | Ig-like and fibronectin type-III | 5.32 | 12.58 | 1.09 | K06774 |
c52952 | Fibronectin type III | 1.68 | 0.64 | −1.18 | GO:0002376 |
Toll pathway | |||||
c57284 | NAD(+) hydrolase sarm1 | 2.31 | 7.01 | 1.56 | GO:0034128 |
c53917 | Toll-like receptor | 0.88 | 2.09 | 1.05 | K10170 |
c54414 | modular serine protease ModSP | 84.80 | 34.60 | −1.29 | K20674 |
c57804 | modular serine protease ModSP | 34.66 | 7.57 | −1.97 | K20674 |
c58774 | Serpin | 76.40 | 21.80 | −1.93 | K13963 |
Ecdysone-mediated | |||||
c55917 | Krueppel 1-like | 3.87 | 16.90 | 1.78 | K09228 |
c56481 | Ecdysone-induced protein | 1.65 | 5.14 | 1.27 | K08701 |
c55601 | PR domain zinc finger protein 1 isoform X1 | 9.68 | 22.20 | 1.06 | GO:0035075 |
Unigene ID | Annotation | FPKM | Log2(FC) b | GO/KO ID | |
---|---|---|---|---|---|
Healthy | Inoculated | ||||
Signaling | |||||
c56897 | inositol 1,4,5-trisphosphate receptor | 0.76 | 2.95 | 1.41 | K04958 |
c56671 | Protein serrate | 1.75 | 5.11 | 1.39 | GO:0007219 |
c58773 | beta-1 adrenergic receptor | 1.70 | 5.26 | 1.39 | GO:0004930 |
c58924 | receptor-type tyrosine-protein phosphatase | 1.89 | 4.44 | 1.21 | K06776 |
c55654 | Rho GTPase-activating | 8.45 | 20.90 | 1.19 | K20655 |
c54354 | ephrin-B1 | 2.89 | 8.17 | 1.17 | K05463 |
c57822 | G-protein coupled receptor | 1.05 | 2.28 | 1.16 | GO:0004930 |
c57839 | FMRFamide receptor | 0.64 | 1.74 | 1.14 | GO:0008528 |
c54122 | WNT1-inducible-signaling pathway | 0.73 | 2.55 | 1.14 | K22471 |
c56120 | G-protein coupled receptor | 0.57 | 1.68 | 1.13 | K08469 |
c56130 | regulator of G-protein signaling | 17.00 | 40.40 | 1.11 | GO:0001965 |
c57791 | dual 3′,5′-cyclic-AMP and -GMP phosphodiesterase | 4.82 | 11.60 | 1.08 | GO:0004114 |
c56800 | JNK-interacting | 4.89 | 10.20 | 1.06 | K20317 |
c54894 | arrestin homolog | 1.55 | 3.98 | 1.05 | K04439 |
Neuromotor system | |||||
c56627 | Neuropilin and tolloid-like | 5.08 | 14.20 | 1.29 | K19404 |
c57981 | unc-80 homolog | 1.16 | 3.76 | 1.28 | K24015 |
c54290 | Neural cell adhesion | 2.55 | 6.57 | 1.11 | K06491 |
c57640 * | synaptotagmin 1 isoform X1 | 7.61 | 17.90 | 1.09 | GO:0007269 |
c56243 | Acetylcholine receptor subunit beta-like | 18.70 | 39.30 | 1.07 | K05312 |
c55778 | neuronal acetylcholine receptor subunit α-7 | 8.41 | 15.10 | 1.07 | GO:0022848 |
c59372 | neurobeachin isoform X3 | 7.78 | 10.90 | 1.04 | K24183 |
c58381 | locomotion-related protein Hikaru genki | 3.02 | 6.64 | 1.04 | K17495 |
c59504 | regulating synaptic membrane exocytosis | 25.30 | 55.90 | 1.03 | K15297 |
c55640 | serine/threonine-protein kinase Nek8 | 1.64 | 3.77 | 1.03 | K20877 |
c57984 | myelin regulatory factor-like | 4.91 | 9.05 | 1.02 | / |
Ion channel | |||||
c58440 | Voltage-gated chloride channel | 2.11 | 14.4 | 2.17 | GO:0005247 |
c58546 | voltage-dependent calcium channel | 0.35 | 1.61 | 1.47 | K04863 |
c58915 | Cyclic nucleotide-gated cation channel α-3 | 4.15 | 10.80 | 1.33 | GO:0005249 |
c55895 | Potassium channel domain | 2.50 | 6.95 | 1.30 | / |
c57081 | Small conductance calcium-activated potassium channel | 2.32 | 5.82 | 1.30 | GO:0016286 |
c56821 | potassium voltage-gated channel | 0.53 | 1.85 | 1.25 | GO:0008076 |
c56460 | Potassium channel | 4.94 | 11.80 | 1.16 | GO:0005267 |
c58522 | potassium voltage-gated channel | 0.66 | 1.77 | 1.12 | K04905 |
c52823 | glutamate-gated chloride channel-like | 17.10 | 7.82 | −1.11 | GO:0005230 |
Unigene ID | Annotation | Accession No. | Unused ProtScore | Coverage (%) | Peptides | TMRs |
---|---|---|---|---|---|---|
c55126 | calcium-transporting ATPase sarcoplasmic/endoplasmic reticulum type isoform X2 | gi|1028717492 | 37.86 | 24.9 | 20 | 8 |
c55766 | AMP deaminase 2 isoform X2 | gi|1028703692 | 12.87 | 9.4 | 7 | 2 |
c57640 | synaptotagmin 1 isoform X1 | gi|641664799 | 9.68 | 14.4 | 5 | 1 |
c58629 | ATP-binding cassette sub-family F member 1 | gi|193676397 | 6.00 | 3 | 3 | 3 |
c52852 | dihydroorotate dehydrogenase (quinone), mitochondrial | gi|641676995 | 5.23 | 8.3 | 3 | 4 |
c55131 | probable hydroxyacid-oxoacid transhydrogenase, mitochondrial | gi|641678166 | 4.21 | 4.1 | 2 | 10 |
c53223 | innexin inx7 | gi|328723643 | 4.08 | 6.7 | 2 | 5 |
c59449 | transmembrane 9 superfamily member 2 | gi|1028716222 | 4.00 | 3.8 | 2 | 9 |
c52282 | ubiquitin-like modifier-activating enzyme 5 | gi|193707023 | 4.00 | 3.4 | 3 | 2 |
c57885 | Mo25 uncharacterized | gi|328720151 | 3.64 | 5.8 | 2 | 6 |
c54081 | tumor suppressor candidate 3 | gi|641672251 | 1.49 | 5.8 | 2 | 6 |
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Zhu, J.; Fu, Y.; Zhang, L.; Yang, T.; Zhou, X. Transcriptomic Profiling of Bean Aphid Megoura crassicauda upon Exposure to the Aphid-Obligate Entomopathogen Conidiobolus obscurus (Entomophthoromycotina) and Screening of CytCo-Binding Aphid Proteins through a Pull-Down Assay. Insects 2024, 15, 388. https://doi.org/10.3390/insects15060388
Zhu J, Fu Y, Zhang L, Yang T, Zhou X. Transcriptomic Profiling of Bean Aphid Megoura crassicauda upon Exposure to the Aphid-Obligate Entomopathogen Conidiobolus obscurus (Entomophthoromycotina) and Screening of CytCo-Binding Aphid Proteins through a Pull-Down Assay. Insects. 2024; 15(6):388. https://doi.org/10.3390/insects15060388
Chicago/Turabian StyleZhu, Jiaqin, Yaqi Fu, Lvhao Zhang, Tian Yang, and Xiang Zhou. 2024. "Transcriptomic Profiling of Bean Aphid Megoura crassicauda upon Exposure to the Aphid-Obligate Entomopathogen Conidiobolus obscurus (Entomophthoromycotina) and Screening of CytCo-Binding Aphid Proteins through a Pull-Down Assay" Insects 15, no. 6: 388. https://doi.org/10.3390/insects15060388