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Insects, Volume 16, Issue 9

2025 September - 121 articles

Cover Story: Honey bee populations face a severe threat from chalkbrood, a lethal disease caused by Ascosphaera apis. Our study identified a key molecular mechanism underlying this infection: the pathogen hijacked the larval apoptotic pathway to induce fatal tissue damage. The fungal infection markedly increased the expression of host pro-apoptotic genes and meanwhile reduced the anti-apoptotic gene expression, triggering extensive cell death. Importantly, pharmacological inhibition of apoptosis significantly enhanced larval survival and decreased the fungal Ste11-like expression. Conversely, artificially inducing apoptosis exacerbated disease progression. These results demonstrate that A. apis actively exploits host apoptosis to promote its own virulence, highlighting the apoptotic pathway as a promising target for therapeutic interventions. View this paper
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Articles (121)

  • Article
  • Open Access
1,081 Views
14 Pages

Overcoming Obstacles: Perspective on How Mediterranean Oaks Defend Their Acorns from Insect Seed Predators

  • David A. Oropesa-Olmedo,
  • Enrique Andivia,
  • Michał Reut,
  • Pablo Cisneros and
  • Raúl Bonal

22 September 2025

Insect pre-dispersal seed predators attack a large proportion of the acorn crops in oak forests worldwide. Oaks (Quercus spp.) have evolved several strategies, including physical barriers, chemical defenses (e.g., tannins), and/or phenological predat...

  • Article
  • Open Access
859 Views
12 Pages

22 September 2025

The effects of Lys and Ile supplementation in a barley-based substrate on growth performance and nutrient deposition in Tenebrio molitor larvae were evaluated. Six trays per treatment containing sixty larvae were assigned to five treatments in two 21...

  • Article
  • Open Access
945 Views
11 Pages

22 September 2025

Tea is a global commodity, and due to its way of preparation pesticide residues cannot be tolerated. This underlines the importance of non-chemical pest control. Kenya is the third largest global tea producer but the potential of natural enemies agai...

  • Article
  • Open Access
846 Views
13 Pages

22 September 2025

Honey robbing, as an extreme adaptive response of honey bee colonies to resource scarcity, poses devastating threats to apiaries, yet the underlying molecular mechanisms remain poorly understood. We compared morphological traits and survival rates be...

  • Article
  • Open Access
649 Views
13 Pages

Improvement and Quantification of Extraction Methods for Annual Bluegrass Weevil Larval Populations

  • Albrecht M. Koppenhöfer,
  • Olga S. Kostromytska and
  • Ana Luiza Sousa

22 September 2025

The annual bluegrass weevil (ABW), Listronotus maculicollis (Kirby), is a significant pest of short-mown turfgrass in eastern North America. Proper monitoring of this pest may reduce insecticide applications. We investigated the extraction rate and l...

  • Article
  • Open Access
1,136 Views
19 Pages

Mouthparts and Alimentary Tract of Flower-Visiting Monkey Beetles (Coleoptera: Scarabaeoidea: Hopliini): Insights into Feeding Preferences

  • Michael Neulinger,
  • Florian Karolyi,
  • Jonathan F. Colville,
  • Myriam E. Widmann,
  • Jonas Kristl and
  • Harald W. Krenn

21 September 2025

Monkey beetles (Hopliini, Scarabaeoidea) are a species-rich group of flower-visiting insects that are specialized to feed on floral tissue, pollen and/or nectar. We studied ten South African species, examining morphological features associated with t...

  • Article
  • Open Access
926 Views
10 Pages

Duplex PCR Detection and Differentiation of Insect DNA Tenebrio molitor and Zophobas morio in Various Types of Food

  • Dagmar Stehlíková,
  • Veronika Müllerová,
  • Anna Adámková,
  • Pavel Beran,
  • Martin Adámek,
  • Vladislav Čurn,
  • Soňa Škrovánková and
  • Jiří Mlček

21 September 2025

Edible insects, particularly Tenebrio molitor (Linnaeus) (mealworm) and Zophobas morio (Fabricius) (superworm), have drawn increasing attention as alternative protein sources. This study aims to develop an accurate molecular detection method for T. m...

  • Article
  • Open Access
1 Citations
938 Views
22 Pages

The Compensatory Response of Photosystem II Photochemistry to Short-Term Insect Herbivory Is Suppressed Under Water Deficit

  • Julietta Moustaka,
  • Ilektra Sperdouli,
  • Stefanos S. Andreadis,
  • Nikoletta Stoikou,
  • Kleoniki Giannousi,
  • Catherine Dendrinou-Samara and
  • Michael Moustakas

21 September 2025

Photosystem II (PSII) is very sensitive to both biotic and abiotic stress conditions, mirroring global climate changes. Crop production worldwide faces rising hazards from the increased duration, frequency, and intensity of drought stress episodes as...

  • Article
  • Open Access
732 Views
14 Pages

Development of an LNA-Based qPCR Assay for Detecting Toumeyella parvicornis (Cockerell, 1897) (Hemiptera: Coccidae) from Insect and Honeydew DNA

  • Domenico Rizzo,
  • Alice Downes,
  • Claudia Gabriela Zubieta,
  • Michela Moriconi,
  • Chiara Ranaldi,
  • Bruno Palmigiano,
  • Antonio Aronadio,
  • Linda Bartolini,
  • Edson Bolige and
  • Elia Russo
  • + 1 author

20 September 2025

The invasive sap-feeding pest Toumeyella parvicornis (pine tortoise scale) is rapidly spreading across Europe, threatening pine ecosystems, particularly in forest–urban areas of Italy. In this scenario, early detection and monitoring strategies...

  • Article
  • Open Access
1 Citations
902 Views
13 Pages

19 September 2025

The relative efficacy of amorphous silica dusts, malathion, and pirimiphos methyl was assessed against S. oryzae and T. castaneum in stored wheat under laboratory conditions. Insecticidal performance was influenced by physical properties such as part...

  • Article
  • Open Access
1 Citations
895 Views
20 Pages

Segmentation of Young Polish Consumers in Relation to Product Attributes and Willingness to Consume Food Containing Edible Insects

  • Anna Platta,
  • Anna Mikulec,
  • Monika Radzymińska,
  • Karolina Mikulec and
  • Stanisław Kowalski

19 September 2025

This study investigated consumer segmentation regarding insect-based foods among Polish Generation Z students. Data from 947 respondents were analyzed using exploratory factor analysis (EFA) followed by k-means clustering. Two segmentation solutions...

  • Article
  • Open Access
765 Views
13 Pages

Developmental Biology and Seasonal Damage of the Grape Borer Xylotrechus pyrrhoderus in Grapevines

  • Ganyu Zhang,
  • Yuying Jia,
  • Haibin Wu,
  • Yong Zhang,
  • Murad Ghanim,
  • Yanan Ma and
  • Ruihong Sun

19 September 2025

Xylotrechus pyrrhoderus Bates (Coleoptera: Cerambycidae) is a major viticultural pest whose larval feeding damages grapevine canes and reduces yields, yet its internal development and damage progression are poorly understood. Using field surveys comb...

  • Article
  • Open Access
868 Views
16 Pages

19 September 2025

The drastic surge in Sardine landings in Korea underscores the urgent need for sustainable upcycling strategies. However, research on the feasibility of using sardine (SD) in food waste (FW)-based substrates during the cultivation of black soldier fl...

  • Article
  • Open Access
1,593 Views
21 Pages

Integrative Study of the Crane Fly Genus Brithura Edwards, 1916 (Diptera: Tipulidae) in East Asia: First Larval Descriptions of the Genus and Insights from Adult Morphology and DNA Barcoding

  • Virginija Podeniene,
  • Sigitas Podenas,
  • Dalius Butkauskas,
  • Donatas Sneideris,
  • Jin Whoa Yum,
  • Neung-Ho Ahn,
  • Soen Yi Kim,
  • Jisoo Kim and
  • Pavel Starkevich

18 September 2025

Brithura Edwards, 1916 (Diptera: Tipulidae) is a small genus of crane flies currently comprising 16 described species distributed across the East Palaearctic and Oriental regions. Although the adults of this genus rank among the largest representativ...

  • Article
  • Open Access
1,260 Views
14 Pages

18 September 2025

Various types of sensilla are densely distributed on the antennal surfaces of insects. The specialization of perception and ecological adaptability of antennae in detecting external environmental signals are reflected in the morphology, function, num...

  • Article
  • Open Access
934 Views
13 Pages

17 September 2025

Bemisia tabaci MED is one of the most invasive and destructive agricultural pests worldwide, posing a serious threat to crop production and biosecurity. Understanding its spatiotemporal population dynamics and genetic structure is critical for early...

  • Article
  • Open Access
1,015 Views
26 Pages

17 September 2025

The genus Homidia contains 84 species of which 60 have been reported from China. The sequence of COI for ten Homidia species are provided and a neighbour-joining tree is presented. Three new species of Homidia are described from Chongqing Municipalit...

  • Article
  • Open Access
884 Views
17 Pages

17 September 2025

Cycads are being endangered by several anthropogenic threats, and invasions of non-native herbivores are among those threats. Various country invasions by the cycad blue butterfly Luthrodes pandava have threatened the local horticulture cycad trade a...

  • Article
  • Open Access
3 Citations
1,940 Views
20 Pages

17 September 2025

The fortification of bakery products with alternative protein sources, including edible insects, offers a promising approach to improving nutritional quality while addressing sustainability challenges. This study evaluated graded replacement of type...

  • Article
  • Open Access
771 Views
13 Pages

17 September 2025

Paracoccus marginatus (Hemiptera: Pseudococcidae) poses a significant threat to over 200 plant species, severely impacting agricultural productivity. Diomus guilavoguii (Coleoptera: Coccinellidae) is a natural predator of P. marginatus. To develop an...

  • Article
  • Open Access
19 Citations
1,239 Views
16 Pages

Response of Nutritional Values and Gut Microbiomes to Dietary Intake of ω-3 Polyunsaturated Fatty Acids in Tenebrio molitor Larvae

  • Aifen Yang,
  • Yiting Ye,
  • Qiwei Liu,
  • Jingjing Xu,
  • Ruixi Li,
  • Mingfeng Xu,
  • Xiu Wang,
  • Sida Fu and
  • Rongrong Yu

16 September 2025

Due to their high nutritional value and a lower environmental impact, Tenebrio molitor (T. molitor) larvae are regarded as an alternative protein and lipid source in food industries, animal husbandry, and fishery. This study aimed to investigate the...

  • Review
  • Open Access
1 Citations
2,342 Views
14 Pages

16 September 2025

Bactrocera dorsalis (Hendel, 1912) (Diptera: Tephritidae) is considered a highly invasive fruit fly species already widespread in Indo-Asia and sub-Saharan Africa. In recent years, this species has extended its range into new regions previously consi...

  • Review
  • Open Access
1,269 Views
22 Pages

Leveraging Adventive and Endemic Parasitoids Against Polyphagous Agromyzid Leafminers in Australia

  • Peter M. Ridland,
  • Elia I. Pirtle,
  • Paul A. Umina and
  • Ary A. Hoffmann

16 September 2025

The recent incursions of three agromyzid leafminers (Liriomyza huidobrensis, L. sativae, and L. trifolii) into Australia pose a significant threat to horticultural production. Outbreaks of these polyphagous pests are consistently associated with the...

  • Article
  • Open Access
1 Citations
1,158 Views
17 Pages

Effect of Diet Compositions on Colony Strength Parameters, and the Enzymatic Activity of Apis mellifera L. During Floral Scarcity

  • Shams Ul Islam,
  • Javeria Liaquat,
  • Muhammad Anjum Aqueel,
  • Asim Abbasi,
  • Muhammad Arshad,
  • Muhammad Shahid Rizwan,
  • Muhammad Saqib,
  • Nasir Masood,
  • Nyasha J. Kavhiza and
  • Abeer Hashem
  • + 4 authors

16 September 2025

Dearth periods associated with less floral resources negatively impact Apis mellifera colony performance. Artificial diets offer nutritional supplements to sustain bee colonies under stressful conditions. An eight-week feeding trial was conducted usi...

  • Communication
  • Open Access
2 Citations
2,381 Views
8 Pages

First Report of Two-Spot Cotton Leafhopper (Amrasca biguttula Ishida) (Hemiptera: Cicadellidae) on Commercial Cotton in the Southeastern United States

  • Isaac L. Esquivel,
  • Tim Bryant,
  • Sean Malone,
  • Alana L. Jacobson,
  • Scott H. Graham,
  • Paulo S. Gimenez-Cremonez,
  • Phillip Roberts,
  • Silvana Paula-Moreas,
  • Dominic Reisig and
  • Sally Taylor
  • + 3 authors

15 September 2025

The two-spot cotton leafhopper, Amrasca biguttula (Ishida, 1913) (Hemiptera: Cicadellidae), is a polyphagous pest native to the Indian subcontinent, where it is a significant pest of cotton (Gossypium spp.), okra (Abelmoschus esculentus, Moench), and...

  • Article
  • Open Access
1 Citations
1,243 Views
11 Pages

Description of a New Species of Mountain Midges (Diptera, Deuterophlebiidae) from Xinjiang, China

  • Xin Wang,
  • Minghui Gao,
  • Xinyang Li,
  • Rui Han,
  • Jiayang Feng and
  • Wei Guo

15 September 2025

The family Deuterophlebiidae is considered to be the most plesiomorphic Diptera family, with a striking morphology and life history. In this study, we provide detailed descriptions and figures of a new species from Northwestern China: Deuterophlebia ...

  • Article
  • Open Access
1,422 Views
46 Pages

15 September 2025

The factors that generate the extraordinary biodiversity of tropical regions are likely multifaceted. In this study, we tested our working hypothesis that a mosaic of contrasting habitats plays a key role in shaping the diversity of tropical pygmy mo...

  • Article
  • Open Access
1 Citations
1,206 Views
14 Pages

13 September 2025

Introduction: With global protein demand rising, edible insects offer a nutrient-rich and environmentally sustainable alternative protein source that could help address both dietary and ecological challenges. This study investigates the attitudes, pe...

  • Article
  • Open Access
1,112 Views
22 Pages

Adaptation to Hot and Humid Climates in the Silkworm: Energy Reallocation and Cuticle Transpiration

  • Jiajun Zhuo,
  • Yuli Zhang,
  • Xing Gao,
  • Cailin Liang,
  • Guizheng Zhang,
  • Lihui Bi,
  • Wei Wei,
  • Shoumin Fang,
  • Xiaoling Tong and
  • Quanyou Yu
  • + 2 authors

12 September 2025

The silkworm (Bombyx mori) is rich in germplasm resources, including thermotolerant strains that live in tropical/subtropical humid climates. In this study, two thermotolerant strains and one sensitive strain were used as materials, with the former e...

  • Article
  • Open Access
976 Views
14 Pages

12 September 2025

Apis cerana cerana is a key social insect, and its ability to recognize chemical signals is crucial for maintaining colony homeostasis and coordinating collective behaviors, such as foraging, nursing, and defense. The legs of insects play a significa...

  • Article
  • Open Access
1,736 Views
14 Pages

12 September 2025

As demand for sustainable protein intensifies, edible crickets offer a promising solution to food insecurity and environmental strain, particularly in regions like Madagascar. This study investigated the effects of two rearing temperatures (28 °C...

  • Article
  • Open Access
1 Citations
1,189 Views
26 Pages

Lightweight Pest Object Detection Model for Complex Economic Forest Tree Scenarios

  • Xiaohui Cheng,
  • Xukun Wang,
  • Yanping Kang,
  • Yun Deng,
  • Qiu Lu,
  • Jian Tang,
  • Yuanyuan Shi and
  • Junyu Zhao

12 September 2025

Pest control in economic forests is a crucial aspect of sustainable forest resource management, yet it faces bottlenecks such as low efficiency and high miss rates for small objects. Based on the RT-DETR model, this paper proposes LightFAD-DETR, a li...

  • Article
  • Open Access
1,260 Views
10 Pages

The Genetic Diversity of the Asian Spongy Moth, Lymantria dispar asiatica Vnukovskii (Lepidoptera: Erebidae), in Korea Based on Mitochondrial COI Analysis

  • Jongmin Bae,
  • Hye-Min Byun,
  • Subin Choi,
  • Geunho Jang,
  • Minjoon Kang,
  • Eunji Kim,
  • Jaekook Park,
  • Heung-Sik Lee and
  • Sunghoon Jung

11 September 2025

In this study, we assessed the genetic diversity of the Asian spongy moth, Lymantria dispar asiatica Vnukovskii (Lepidoptera: Erebidae), in Korea. We obtained 123 sequences of the species, including those from 26 regions in Korea. We analyzed the gen...

  • Review
  • Open Access
1 Citations
978 Views
14 Pages

11 September 2025

Global climate change has intensified temperature fluctuations, significantly impacting insect populations. Thermal tolerance has emerged as a critical determinant of species distribution and invasion potential. Liriomyza trifolii, an economically im...

  • Article
  • Open Access
1,051 Views
26 Pages

Recurrent Duplication, Testis-Biased Expression, and Functional Diversification of Esf2/ABT1 Family Genes in Drosophila

  • Elizaveta D. Davydova,
  • Alexei A. Kotov,
  • Alina V. Chernizova,
  • Ekaterina Yu. Yakovleva and
  • Ludmila V. Olenina

11 September 2025

Gene duplications are considered to be the major evolutionary resource of novel functions. The gene family Esf2/ABP1 is conserved in metazoan organisms from yeast to humans. Here we performed a search and characterization of Esf2/ABP1 homologs in the...

  • Article
  • Open Access
1,107 Views
14 Pages

Evaluating the Effectiveness of Insecticides on Spotted Lanternfly Lycorma delicatula (Hemiptera: Fulgoridae) in Kiwifruit

  • Zi-Jian Song,
  • Yi-Na Bai,
  • Zheng-Yu Luo,
  • Rebecca Burns,
  • Chandan Pal,
  • Feng Zhang,
  • Shu-Sen Shi,
  • Rui Bi and
  • Jin-Ping Zhang

11 September 2025

The spotted lanternfly (SLF), Lycorma delicatula (Hemiptera: Fulgoridae), has invaded several countries and caused significant losses to agricultural economy, especially in grapes, apples and kiwifruit production. Currently, chemical control is the p...

  • Article
  • Open Access
1,074 Views
26 Pages

Taxonomic Review of the Genus Taxigramma (Diptera, Sarcophagidae, Miltogramminae) from China

  • Zijun Liu,
  • Yi Gai,
  • Xiaolan Cheng,
  • Hanqing Li,
  • Yaqian Fan,
  • Krzysztof Szpila,
  • Thomas Pape and
  • Dong Zhang

11 September 2025

The genus Taxigramma from China has been taxonomically reviewed. Taxigramma pluriseta and T. pluton are new to the fauna sinica, while T. karakulensis is removed. The male of T. albina is firstly recorded and described from China. An updated checklis...

  • Article
  • Open Access
959 Views
21 Pages

11 September 2025

Spodoptera frugiperda is a major transboundary migratory pest under global alert by the Food and Agriculture Organization (FAO) of the United Nations. The accurate identification and counting of trapped adults in the field are key technologies for ac...

  • Article
  • Open Access
2 Citations
1,065 Views
14 Pages

Lipid Composition Analysis of Cricket Oil from Crickets Fed with Broken Rice-Derived Bran

  • Ryosuke Sogame,
  • Taiki Miyazawa,
  • Masako Toda,
  • Akihiro Iijima,
  • Maharshi Bhaswant and
  • Teruo Miyazawa

11 September 2025

This study investigated the effect of rice bran derived from broken rice feeding on the lipid classes and fatty acid composition of oil extracted from crickets (Acheta domesticus). During the final seven days prior to cricket oil extraction, crickets...

  • Article
  • Open Access
813 Views
17 Pages

Integrative Morphological and Molecular Diagnostics for Euseius nicholsi and Euseius oolong (Acari: Phytoseiidae)

  • Xiaoduan Fang,
  • Jun Li,
  • Syed Usman Mahmood,
  • Nwanade Chuks Fidelis and
  • Jianglei Meng

10 September 2025

In a survey of Bajiaozhai National Forest Park (Guilin, China), several specimens of an Euseius sp. were collected. These specimens were very similar to Euseius nicholsi and Euseius oolong, based on morphological observations. However, some morpholog...

  • Article
  • Open Access
1,121 Views
13 Pages

10 September 2025

Rice (Oryza sativa) provides a major food resource worldwide, playing an important role in the global economy. Leptocorisa acuta (Hemiptera: Alydidae), commonly known as the rice seed bug, is a major pest of paddy crops in many rice-growing regions a...

  • Article
  • Open Access
788 Views
13 Pages

Non-Target Effects of Beta-Cypermethrin on Baryscapus dioryctriae and Ecological Risk Assessment

  • Jing Li,
  • Tongtong Zuo,
  • Sicheng Fei,
  • Yuequ Chen,
  • Xiangyu Zhang,
  • Qi Chen,
  • Liwen Song and
  • Kaipeng Zhang

10 September 2025

Beta-cypermethrin is widely applied in Korean pine (Pinus koraiensis Siebold & Zucc.) seed orchards to control cone- and seed-infesting moths (e.g., Dioryctria spp.), yet its Wsublethal risks to non-target beneficial arthropods remain insufficien...

  • Article
  • Open Access
1,066 Views
8 Pages

10 September 2025

All solitary and primitively eusocial bees of the temperate zones survive winter in a dormant diapausing state. Weight loss of Osmia and Megachile bees has been solely attributed to metabolism of stored triglycerides, as evidenced by loss of fat body...

  • Review
  • Open Access
1 Citations
1,796 Views
22 Pages

9 September 2025

Dragonflies and damselflies (Odonata) play important roles in ecology and are used as model organisms in evolutionary studies, but a comprehensive overview of Odonata research has been lacking. Here, we conducted a bibliometric analysis of Odonata li...

  • Article
  • Open Access
638 Views
14 Pages

Taxonomic Revision of D. carlosvilelai Vela and Rafael, 2001, and Updated Record of D. paraguayensis Duda, 1927 Registry in Ecuador

  • Coraima Elizabeth Suárez,
  • Ana Peñafiel-Vinueza,
  • Violeta Rafael,
  • María Isabel Tamayo and
  • Doris Vela

9 September 2025

Drosophila carlosvilelai and D. paraguayensis are species that were collected in the Pasochoa Protected Forest between 1996 and 1997. D. carlosvilelai was described incompletely as a new species from Ecuador. Regarding D. paraguayensis, recent collec...

  • Article
  • Open Access
2 Citations
4,295 Views
14 Pages

Anaxyelidae of Karatau: 100 Years After

  • Dmitry S. Kopylov and
  • Alexandr P. Rasnitsyn

9 September 2025

The fossil beds of the Karabastau Formation in southern Kazakhstan, known as the “Jurassic Lake of Karatau”, represent one of the richest Lagerstätten for Jurassic insects, with more than 800 described species. This year marks the ce...

  • Review
  • Open Access
1,056 Views
19 Pages

Advances in the Molecular Mechanisms of Resistance in Chilo suppressalis

  • Wenchao Ge,
  • Guanghang Chen,
  • Mengzhen Wang,
  • Shunfan Wu and
  • Congfen Gao

8 September 2025

The rice stem borer, Chilo suppressalis (Walker) (Lepidoptera: Crambidae), is one of the major pests in rice-growing areas. Its larvae feed on rice stems, causing symptoms of rice dead sheaths, dead hearts, and withered ears, resulting in heavy rice...

  • Article
  • Open Access
3,657 Views
18 Pages

7 September 2025

The beetle family Cerophytidae is reported from China for the first time, based on the discovery of Cerophytum lii Qiu & Ruan, sp. nov. and Cerophytum sp., both from Daweishan Mountain in Southeast Yunnan Province. Detailed descriptions and diagn...

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Insects - ISSN 2075-4450