ERG3-Encoding Sterol C5,6-DESATURASE in Candida albicans Is Required for Virulence in an Enterically Infected Invasive Candidiasis Mouse Model
Abstract
:1. Introduction
2. Results
2.1. Loss of ERG3 in C. albicans Results in Attenuated Virulence in an Enterically Infected Invasive Candidiasis Mouse Model
2.2. C. albicans erg3 Null Mutant Has a Lower Colonization and Dissemination Capacity in the Gut
2.3. Chemokines Levels in the Gut and Blood of Mice Infected with the erg3 Null Mutant Were Significantly Lower Than Those in Mice Infected with the Wild-Type Strain
2.4. Histopathological Examination
3. Discussion
4. Materials and Methods
4.1. Candida Strains and Culture Conditions
4.2. Animals and Ethics Statement
4.3. Murine Model of Candidiasis from the Gut
4.4. Isolation of Candida Cells from the Blood, Liver, Kidney, and Stool
4.5. Chemokine Detection Assay
4.6. Histopathological Examination
4.7. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Hirayama, T.; Miyazaki, T.; Sumiyoshi, M.; Ashizawa, N.; Takazono, T.; Yamamoto, K.; Imamura, Y.; Izumikawa, K.; Yanagihara, K.; Kohno, S.; et al. ERG3-Encoding Sterol C5,6-DESATURASE in Candida albicans Is Required for Virulence in an Enterically Infected Invasive Candidiasis Mouse Model. Pathogens 2021, 10, 23. https://doi.org/10.3390/pathogens10010023
Hirayama T, Miyazaki T, Sumiyoshi M, Ashizawa N, Takazono T, Yamamoto K, Imamura Y, Izumikawa K, Yanagihara K, Kohno S, et al. ERG3-Encoding Sterol C5,6-DESATURASE in Candida albicans Is Required for Virulence in an Enterically Infected Invasive Candidiasis Mouse Model. Pathogens. 2021; 10(1):23. https://doi.org/10.3390/pathogens10010023
Chicago/Turabian StyleHirayama, Tatsuro, Taiga Miyazaki, Makoto Sumiyoshi, Nobuyuki Ashizawa, Takahiro Takazono, Kazuko Yamamoto, Yoshifumi Imamura, Koichi Izumikawa, Katsunori Yanagihara, Shigeru Kohno, and et al. 2021. "ERG3-Encoding Sterol C5,6-DESATURASE in Candida albicans Is Required for Virulence in an Enterically Infected Invasive Candidiasis Mouse Model" Pathogens 10, no. 1: 23. https://doi.org/10.3390/pathogens10010023