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Article

Expression of HIF-1α and Genes Involved in Glucose Metabolism Is Increased in Cervical Cancer and HPV-16-Positive Cell Lines

by
Víctor D. Priego-Hernández
1,
Adán Arizmendi-Izazaga
1,
Diana G. Soto-Flores
1,
Norma Santiago-Ramón
1,
Milagros D. Feria-Valadez
1,
Napoleón Navarro-Tito
2,
Hilda Jiménez-Wences
3,4,
Dinorah N. Martínez-Carrillo
3,4,
Eric G. Salmerón-Bárcenas
5,
Marco A. Leyva-Vázquez
1,
Berenice Illades-Aguiar
1,
Luz del C. Alarcón-Romero
6 and
Julio Ortiz-Ortiz
1,4,*
1
Laboratorio de Biomedicina Molecular, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Av. Lázaro Cárdenas S/N, Ciudad Universitaria, Colonia La Haciendita, Chilpancingo C.P. 39090, Guerrero, Mexico
2
Laboratorio de Biología Celular del Cáncer, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Av. Lázaro Cárdenas S/N, Ciudad Universitaria, Colonia La Haciendita, Chilpancingo C.P. 39090, Guerrero, Mexico
3
Laboratorio de Investigación Clínica, Facultad de Ciencias, Químico Biológicas, Universidad Autónoma de Guerrero, Av. Lázaro Cárdenas S/N, Ciudad Universitaria, Colonia La Haciendita, Chilpancingo C.P. 39090, Guerrero, Mexico
4
Laboratorio de Investigación en Biomoléculas, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Av. Lázaro Cárdenas S/N, Ciudad Universitaria, Colonia La Haciendita, Chilpancingo C.P. 39090, Guerrero, Mexico
5
Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de Mexico 07360, Mexico
6
Laboratorio de Investigación en Citopatología e Histoquímica de la Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo C.P. 39090, Guerrero, Mexico
*
Author to whom correspondence should be addressed.
Pathogens 2023, 12(1), 33; https://doi.org/10.3390/pathogens12010033
Submission received: 14 November 2022 / Revised: 21 December 2022 / Accepted: 23 December 2022 / Published: 25 December 2022
(This article belongs to the Special Issue Latest Advances in Human Papillomavirus (HPV) Research)
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Abstract

Cervical cancer (CC) is the most common cancer in women in the lower genital tract. The main risk factor for developing CC is persistent infection with HPV 16. The E6 and E7 oncoproteins of HPV 16 have been related to metabolic reprogramming in cancer through the regulation of the expression and stability of HIF-1α and consequently of the expression of its target genes, such as HIF1A (HIF-1α), SLC2A1 (GLUT1), LDHA, CA9 (CAIX), SLC16A3 (MCT4), and BSG (Basigin or CD147), which are involved in glucose metabolism. This work aimed to evaluate the expression of HIF-1α, GLUT1, LDHA, CAIX, MCT4, and Basigin in patient samples and CC cell lines. To evaluate the expression level of HIF1A, SLC2A1, LDHA, CA9, SLC16A3, and BSG genes in tissue from patients with CC and normal tissue, the TCGA dataset was used. To evaluate the expression level of these genes by RT-qPCR in CC cell lines, HPV-negative (C-33A) and HPV-16-positive (SiHa and Ca Ski) cell lines were used. Increased expression of HIF1A, SLC2A1, LDHA, SLC16A3, and BSG was found in Ca Ski and CA9 in SiHa compared to C-33A. Similar results were observed in CC tissues compared to normal tissue obtained by bioinformatics analysis. In conclusion, the expression of HIF-1α, GLUT1, LDHA, CAIX, MCT4, and BSG genes is increased in CC and HPV-16-positive cell lines.
Keywords: HPV 16; HIF-1α; glucose metabolism; metabolic reprogramming; cervical cancer HPV 16; HIF-1α; glucose metabolism; metabolic reprogramming; cervical cancer

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MDPI and ACS Style

Priego-Hernández, V.D.; Arizmendi-Izazaga, A.; Soto-Flores, D.G.; Santiago-Ramón, N.; Feria-Valadez, M.D.; Navarro-Tito, N.; Jiménez-Wences, H.; Martínez-Carrillo, D.N.; Salmerón-Bárcenas, E.G.; Leyva-Vázquez, M.A.; et al. Expression of HIF-1α and Genes Involved in Glucose Metabolism Is Increased in Cervical Cancer and HPV-16-Positive Cell Lines. Pathogens 2023, 12, 33. https://doi.org/10.3390/pathogens12010033

AMA Style

Priego-Hernández VD, Arizmendi-Izazaga A, Soto-Flores DG, Santiago-Ramón N, Feria-Valadez MD, Navarro-Tito N, Jiménez-Wences H, Martínez-Carrillo DN, Salmerón-Bárcenas EG, Leyva-Vázquez MA, et al. Expression of HIF-1α and Genes Involved in Glucose Metabolism Is Increased in Cervical Cancer and HPV-16-Positive Cell Lines. Pathogens. 2023; 12(1):33. https://doi.org/10.3390/pathogens12010033

Chicago/Turabian Style

Priego-Hernández, Víctor D., Adán Arizmendi-Izazaga, Diana G. Soto-Flores, Norma Santiago-Ramón, Milagros D. Feria-Valadez, Napoleón Navarro-Tito, Hilda Jiménez-Wences, Dinorah N. Martínez-Carrillo, Eric G. Salmerón-Bárcenas, Marco A. Leyva-Vázquez, and et al. 2023. "Expression of HIF-1α and Genes Involved in Glucose Metabolism Is Increased in Cervical Cancer and HPV-16-Positive Cell Lines" Pathogens 12, no. 1: 33. https://doi.org/10.3390/pathogens12010033

APA Style

Priego-Hernández, V. D., Arizmendi-Izazaga, A., Soto-Flores, D. G., Santiago-Ramón, N., Feria-Valadez, M. D., Navarro-Tito, N., Jiménez-Wences, H., Martínez-Carrillo, D. N., Salmerón-Bárcenas, E. G., Leyva-Vázquez, M. A., Illades-Aguiar, B., Alarcón-Romero, L. d. C., & Ortiz-Ortiz, J. (2023). Expression of HIF-1α and Genes Involved in Glucose Metabolism Is Increased in Cervical Cancer and HPV-16-Positive Cell Lines. Pathogens, 12(1), 33. https://doi.org/10.3390/pathogens12010033

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