Molecular Identification of Extrapulmonary Vaccine Adverse Events after BCG in Paraffin-Embedded Specimens
Abstract
:1. Introduction
2. Methods
2.1. Pathomorphological Examination
2.2. Genetic Analysis
- ○
- The formalin-fixed, paraffin-embedded tissues were deparaffinised and then lysed (with proteinase K). DNA was extracted in an automated system Maxwell Promega (16 FFPE Plus Lev DNA Purification Kit);
- ○
- The genetic material was then analysed by: Xpert MTB/RIF Ultra (Cepheid https://www.cepheid.com/content/dam/www-cepheid-com/documents/package-insert-files/Xpert-MTB-RIF-ENGLISH-Package-Insert-301-1404-Rev-G.pdf, accessed on 1 July 2020—detection of specific sequences to MTBC;
- ○
- GenoType MTBC;
- ○
- Spoliogtyping (Spoligotyping Kit Manual, Isogen Life Science)—identification of Mycobacterium bovis BCG.
2.3. Culturing Methods
3. Case I
4. Case II
5. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
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Brzezińska, S.; Zabost, A.; Borkowska-Tatar, D.; Klatt, M.; Goździk, J.; Dłużniewska, A.; Błasińska, K.; Augustynowicz-Kopeć, E. Molecular Identification of Extrapulmonary Vaccine Adverse Events after BCG in Paraffin-Embedded Specimens. Pathogens 2023, 12, 1374. https://doi.org/10.3390/pathogens12121374
Brzezińska S, Zabost A, Borkowska-Tatar D, Klatt M, Goździk J, Dłużniewska A, Błasińska K, Augustynowicz-Kopeć E. Molecular Identification of Extrapulmonary Vaccine Adverse Events after BCG in Paraffin-Embedded Specimens. Pathogens. 2023; 12(12):1374. https://doi.org/10.3390/pathogens12121374
Chicago/Turabian StyleBrzezińska, Sylwia, Anna Zabost, Dagmara Borkowska-Tatar, Magdalena Klatt, Jolanta Goździk, Agnieszka Dłużniewska, Katarzyna Błasińska, and Ewa Augustynowicz-Kopeć. 2023. "Molecular Identification of Extrapulmonary Vaccine Adverse Events after BCG in Paraffin-Embedded Specimens" Pathogens 12, no. 12: 1374. https://doi.org/10.3390/pathogens12121374