Type I Cystatin Derived from Fasciola gigantica Suppresses Macrophage-Mediated Inflammatory Responses
Abstract
:1. Introduction
2. Materials and Methods
2.1. Production of Recombinant FgCyst (rFgCyst) and Endotoxin Removal
2.2. Cell Culture and Treatments
2.3. Cell Viability Assay
2.4. Western Blot Analysis
2.5. Reverse Transcription Real-Time PCR
2.6. Evaluation of Cytokine Secretion by ELISA Assay
2.7. Evaluation of NO by Griess Test
2.8. Statistical Analysis
3. Results
3.1. Cytotoxicity of rFgCyst
3.2. Inhibitory Effect of rFgCyst on LPS-Induced Inflammatory mRNA Expression
3.3. Inhibitory Effect of rFgCyst on the Expression of Proinflammatory Cytokines in LPS-Stimulated Macrophages
3.4. Inhibitory Effect of rFgCyst on the Production of Soluble Inflammatory Cytokines in LPS-Stimulated Macrophages
3.5. Inhibitory Effect of rFgCyst on NO Production in LPS-Stimulated Macrophages
3.6. Inhibitory Effect of rFgCyst on Inflammation Involves the Alteration of Nuclear Factor Kappa B (NF-κB) Signaling Pathway in LPS-Activated Macrophages
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cell | Gene | Accession No. | Primer Sequence from 5′ to 3′ | Product Size (bp) | |
---|---|---|---|---|---|
THP-1 | IL-1β | NM_000576.3 | FW: | CTG AGC TCG CCA GTG AAA TG | 202 |
RV: | TCC ATG GCC ACA ACA ACT GA | ||||
IL-6 | NM_000600.5 | FW: | ACT CAC CTC TTC AGA ACG AAT TG | 149 | |
RV: | CCA TCT TTG GAA GGT TCA GGT TG | ||||
IL-8 | NM_001354840.3 | FW: | ACT GAG AGT GAT TGA GAG TGG AC | 112 | |
RV: | AAC CCT CTG CAC CCA GTT TTC | ||||
TNF-α | NM_000594.4 | FW: | GAG GCC AAG CCC TGG TAT G | 91 | |
RV: | CGG GCC GAT TGA TCT CAG C | ||||
COX-2 | NM_000963.40 | FW: | CAG CAC TTC ACG CAT CAG TT | 128 | |
RV: | CGC AGT TTA CGC TGT CTA GC | ||||
iNOS | NM_000625.4 | FW: | CGC ATG ACC TTG GTG TTT GG | 142 | |
RV: | CAT AGA CCT TGG GGC TTG CCA | ||||
GAPDH | NM_001289745.3 | FW: | GAG TCA ACG GAT TTG GTC GT | 214 | |
RV: | TGG AAG ATG GTG ATG GGA TT | ||||
RAW 264.7 | IL-1β | NM_008361.4 | FW: | TGC CAC CCT TTT GAC AGT GAT G | 138 |
RV: | TGA TGT GCT GCT GCG AGA TT | ||||
IL-6 | NM_031168.2 | FW: | CCC CAA TTT CCA ATG CTC TCC | 141 | |
RV: | CGC ACT AGG TTT GCC GAG TA | ||||
TNF-α | NM_013693.3 | FW: | CCC TCA CAC TCA GAT CAT CTT CT | 61 | |
RV: | GCT ACG ACG TGG GCT ACA G | ||||
COX-2 | NM_011198.4 | FW: | TGT GAC TGT ACC CGG ACT GG | 233 | |
RV: | TGC ACA TTG TAA GTA GGT GGA C | ||||
iNOS | NM_001313922.1 | FW: | CCC TTC CGA AGT TTC TGG CAG CAG | 497 | |
RV: | GGC TGT CAG AGC CTC GTG GCT TTG G | ||||
GAPDH | NM_001289726.2 | FW: | CGA CTT CAA CAG CGA CAC TCA C | 119 | |
RV: | CCC TGT TGC TGT AGC CAA ATT C |
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Chantree, P.; Tarasuk, M.; Prathaphan, P.; Ruangtong, J.; Jamklang, M.; Chumkiew, S.; Martviset, P. Type I Cystatin Derived from Fasciola gigantica Suppresses Macrophage-Mediated Inflammatory Responses. Pathogens 2023, 12, 395. https://doi.org/10.3390/pathogens12030395
Chantree P, Tarasuk M, Prathaphan P, Ruangtong J, Jamklang M, Chumkiew S, Martviset P. Type I Cystatin Derived from Fasciola gigantica Suppresses Macrophage-Mediated Inflammatory Responses. Pathogens. 2023; 12(3):395. https://doi.org/10.3390/pathogens12030395
Chicago/Turabian StyleChantree, Pathanin, Mayuri Tarasuk, Parisa Prathaphan, Jittiporn Ruangtong, Mantana Jamklang, Sirilak Chumkiew, and Pongsakorn Martviset. 2023. "Type I Cystatin Derived from Fasciola gigantica Suppresses Macrophage-Mediated Inflammatory Responses" Pathogens 12, no. 3: 395. https://doi.org/10.3390/pathogens12030395