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Volume 12
Issue 4
10.3390/pathogens12040586
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Article

The Non-Canonical Iron-Responsive Element of IRE-tvcp12 Hairpin Structure at the 3′-UTR of Trichomonas vaginalis TvCP12 mRNA That Binds TvHSP70 and TvACTN-3 Can Regulate mRNA Stability and Amount of Protein

by
Claudia R. León-Sicairos
,
Elisa E. Figueroa-Angulo
,
Jaeson S. Calla-Choque
and
Rossana Arroyo
*
Department of Infectomics and Molecular Pathogenesis, Center of Research and Advanced Studies of IPN (CINVESTAV-IPN), Av. IPN # 2508, Col. San Pedro Zacatenco, Mexico City 07360, Mexico
*
Author to whom correspondence should be addressed.
Pathogens 2023, 12(4), 586; https://doi.org/10.3390/pathogens12040586
Submission received: 24 February 2023 / Revised: 31 March 2023 / Accepted: 3 April 2023 / Published: 12 April 2023
(This article belongs to the Special Issue Mechanisms of Gene Expression Regulation during Host-Pathogen Interaction)
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Abstract

Trichomonas vaginalis is one of the most common sexually transmitted parasites in humans. This protozoan has high iron requirements for growth, metabolism, and virulence. However, iron concentrations also differentially modulate T. vaginalis gene expression as in the genes encoding cysteine proteinases TvCP4 and TvCP12. Our goal was to identify the regulatory mechanism mediating the upregulation of tvcp12 under iron-restricted (IR) conditions. Here, we showed by RT-PCR, Western blot, and immunocytochemistry assays that IR conditions increase mRNA stability and amount of TvCP12. RNA electrophoretic mobility shift assay (REMSA), UV cross-linking, and competition assays demonstrated that a non-canonical iron-responsive element (IRE)-like structure at the 3′-untranslated region of the tvcp12 transcript (IRE-tvcp12) specifically binds to human iron regulatory proteins (IRPs) and to atypical RNA-binding cytoplasmic proteins from IR trichomonads, such as HSP70 and α-Actinin 3. These data were confirmed by REMSA supershift and Northwestern blot assays. Thus, our findings show that a positive gene expression regulation under IR conditions occurs at the posttranscriptional level possibly through RNA-protein interactions between atypical RNA-binding proteins and non-canonical IRE-like structures at the 3′-UTR of the transcript by a parallel mechanism to the mammalian IRE/IRP system that can be applied to other iron-regulated genes of T. vaginalis.
Keywords: atypical RNA-binding proteins; TvCP12; non-canonical iron-responsive element-tvcp12 (IRE-tvcp12); IRE/IRP system; mRNA stability; Northwestern blot assay; posttranscriptional iron regulation; REMSA; Trichomonas vaginalis; UV cross-linking assay atypical RNA-binding proteins; TvCP12; non-canonical iron-responsive element-tvcp12 (IRE-tvcp12); IRE/IRP system; mRNA stability; Northwestern blot assay; posttranscriptional iron regulation; REMSA; Trichomonas vaginalis; UV cross-linking assay
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MDPI and ACS Style

León-Sicairos, C.R.; Figueroa-Angulo, E.E.; Calla-Choque, J.S.; Arroyo, R. The Non-Canonical Iron-Responsive Element of IRE-tvcp12 Hairpin Structure at the 3′-UTR of Trichomonas vaginalis TvCP12 mRNA That Binds TvHSP70 and TvACTN-3 Can Regulate mRNA Stability and Amount of Protein. Pathogens 2023, 12, 586. https://doi.org/10.3390/pathogens12040586

AMA Style

León-Sicairos CR, Figueroa-Angulo EE, Calla-Choque JS, Arroyo R. The Non-Canonical Iron-Responsive Element of IRE-tvcp12 Hairpin Structure at the 3′-UTR of Trichomonas vaginalis TvCP12 mRNA That Binds TvHSP70 and TvACTN-3 Can Regulate mRNA Stability and Amount of Protein. Pathogens. 2023; 12(4):586. https://doi.org/10.3390/pathogens12040586

Chicago/Turabian Style

León-Sicairos, Claudia R., Elisa E. Figueroa-Angulo, Jaeson S. Calla-Choque, and Rossana Arroyo. 2023. "The Non-Canonical Iron-Responsive Element of IRE-tvcp12 Hairpin Structure at the 3′-UTR of Trichomonas vaginalis TvCP12 mRNA That Binds TvHSP70 and TvACTN-3 Can Regulate mRNA Stability and Amount of Protein" Pathogens 12, no. 4: 586. https://doi.org/10.3390/pathogens12040586

APA Style

León-Sicairos, C. R., Figueroa-Angulo, E. E., Calla-Choque, J. S., & Arroyo, R. (2023). The Non-Canonical Iron-Responsive Element of IRE-tvcp12 Hairpin Structure at the 3′-UTR of Trichomonas vaginalis TvCP12 mRNA That Binds TvHSP70 and TvACTN-3 Can Regulate mRNA Stability and Amount of Protein. Pathogens, 12(4), 586. https://doi.org/10.3390/pathogens12040586

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