Carriage Rate of Enterobacterales Resistant to Extended-Spectrum Cephalosporins in the Tunisian Population
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics
2.2. Study Design, Bacterial Isolation and Identification
2.3. Antibiotic Susceptibility Testing
2.4. Molecular Typing of the Isolates
2.5. Short-Read Whole-Genome Sequencing and Genomic Analyses
2.6. Phylogenetic Analysis
2.7. Long-Read Sequencing
2.8. Data Availability
3. Results
3.1. Characterization of Healthy Volunteers and Carriage Rate of ESC-Resistant Enterobacterales
3.2. Resistance Phenotypes and Genotypes
3.3. Characterization of ESC-Resistant Enterobacterales
3.4. Characterization of the Genetic Determinants Carrying ESBL/AmpC Genes
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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E. coli (n = 24) | ||
---|---|---|
No. of Strains | % of Resistance | |
Kanamycin | 2 | 8.3 |
Tobramycin | 1 | 4.2 |
Gentamicin | 1 | 4.2 |
Apramycin | 0 | 0.0 |
Streptomycin | 14 | 58.3 |
Amikacin | 0 | 0.0 |
Netilmicin | 0 | 0.0 |
Tetracycline | 18 | 75.0 |
Chloramphenicol | 2 | 8.3 |
Florfenicol | 2 | 8.3 |
Colistin | 0 | 0.0 |
Nalidixic acid | 2 | 8.3 |
Ciprofloxacin | 1 | 4.2 |
Trimethoprim | 18 | 75.0 |
Sulfonamides | 18 | 75.0 |
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Mahjoub Khachroub, A.; Souguir, M.; Châtre, P.; Elhouda Bouhlel, N.; Jaidane, N.; Drapeau, A.; El Kantaoui, M.; Azaiez, S.; Madec, J.-Y.; Mansour, W.; et al. Carriage Rate of Enterobacterales Resistant to Extended-Spectrum Cephalosporins in the Tunisian Population. Pathogens 2024, 13, 624. https://doi.org/10.3390/pathogens13080624
Mahjoub Khachroub A, Souguir M, Châtre P, Elhouda Bouhlel N, Jaidane N, Drapeau A, El Kantaoui M, Azaiez S, Madec J-Y, Mansour W, et al. Carriage Rate of Enterobacterales Resistant to Extended-Spectrum Cephalosporins in the Tunisian Population. Pathogens. 2024; 13(8):624. https://doi.org/10.3390/pathogens13080624
Chicago/Turabian StyleMahjoub Khachroub, Ahlem, Meriem Souguir, Pierre Châtre, Nour Elhouda Bouhlel, Nadia Jaidane, Antoine Drapeau, Marah El Kantaoui, Sana Azaiez, Jean-Yves Madec, Wejdene Mansour, and et al. 2024. "Carriage Rate of Enterobacterales Resistant to Extended-Spectrum Cephalosporins in the Tunisian Population" Pathogens 13, no. 8: 624. https://doi.org/10.3390/pathogens13080624