Postmortem Sampling in Piglet Populations: Unveiling Specimens Accuracy for Porcine Reproductive and Respiratory Syndrome Detection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Design
2.2. Sampling and Testing
2.3. Data Analysis
2.4. Ethics Statement
3. Results
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Farm 1 Visit 1 (F1V1) | Farm 1 Visit 2 (F1V2) | Farm 2 Visit 1 (F2V1) | Farm 2 Visit 2 (F2V2) | Farm 3 Visit 1 (F3V1) | Farm 3 Visit 2 (F3V2) | |
---|---|---|---|---|---|---|
Days post-outbreak | 63 | 140 | 55 | 140 | 56 | 147 |
Live piglets | ||||||
Serum | 23/29 (79.31%) | 6/60(10.00%) | 19/30 (63.33%) | 0/60 (0.00%) | 9/30 (30.00%) | 6/60 (10.00%) |
Post-mortem sampling | ||||||
Serum | 14/30 (46.67%) | 6/33 (18.18%) | 12/16 (75.00%) | 1/17 (5.88%) | 23/31 (74.19%) | 0/59 (0.00%) |
OS | 15/30 (50.00%) | 9/38 (23.68%) | 15/17 (88.24%) | 0/20 (0.00%) | 28/31 (90.32%) | 9/60 (15.00%) |
NS | 14/30 (46.67%) | 7/38 (18.42%) | 14/17 (82.35%) | 0/20 (0.00%) | 28/31 (90.32%) | 10/60 (16.67%) |
RS | 13/30 (43.33%) | 5/38 (13.16%) | 12/17 (70.59%) | 0/20 (0.00%) | 28/31 (90.32%) | 12/60 (20.00%) |
TTF | 25/30 (83.33%) | 21/36 (58.33%) | 15/17 (88.24%) | 2/19 (10.53%) | 31/31 (100.00%) | 24/60 (40.00%) |
SIL | NA | NA | NA | NA | 26/31 (83.87%) | 7/60 (11.67%) |
TP | FN | FP | TN | Sensitivity (95% CI) | Specificity (95% CI) | PPV (95% CI) | NPV (95% CI) | |
---|---|---|---|---|---|---|---|---|
OS | 53 | 3 | 21 | 109 | 94.64% (85.13–98.88%) | 83.85% (76.37–89.71%) | 71.62% (59.95–81.50%) | 97.32% (92.37–99.44%) |
NS | 50 | 6 | 21 | 109 | 89.29% (78.12–95.97%) | 83.85% (76.37–89.71%) | 70.42% (58.41–80.67%) | 94.78% (88.99–98.06%) |
RS | 48 | 8 | 20 | 110 | 85.71% (73.78–93.62%) | 84.62% (77.24–90.34%) | 70.59% (58.29–81.02%) | 93.22% (87.08–97.03%) |
TTF | 55 | 1 | 59 | 69 | 98.21% (90.45–99.95%) | 53.91% (44.88–62.75%) | 48.25% (38.79–57.80%) | 98.57% (92.30–99.96%) |
SIL | 23 | 0 | 10 | 57 | 100.00% (85.18–100.00%) | 85.07% (74.26–92.60%) | 69.70% (51.29–84.41%) | 100.00% (93.73–100.00%) |
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Kikuti, M.; Melini, C.M.; Yue, X.; Culhane, M.; Corzo, C.A. Postmortem Sampling in Piglet Populations: Unveiling Specimens Accuracy for Porcine Reproductive and Respiratory Syndrome Detection. Pathogens 2024, 13, 649. https://doi.org/10.3390/pathogens13080649
Kikuti M, Melini CM, Yue X, Culhane M, Corzo CA. Postmortem Sampling in Piglet Populations: Unveiling Specimens Accuracy for Porcine Reproductive and Respiratory Syndrome Detection. Pathogens. 2024; 13(8):649. https://doi.org/10.3390/pathogens13080649
Chicago/Turabian StyleKikuti, Mariana, Claudio Marcello Melini, Xiaomei Yue, Marie Culhane, and Cesar A. Corzo. 2024. "Postmortem Sampling in Piglet Populations: Unveiling Specimens Accuracy for Porcine Reproductive and Respiratory Syndrome Detection" Pathogens 13, no. 8: 649. https://doi.org/10.3390/pathogens13080649