Utilization of In Vivo Imaging System to Study Staphylococcal Sepsis and Septic Arthritis Progression in Mouse Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics Statement
2.2. Mice
2.3. Bacterial Strains
2.4. Mouse Models of S. aureus Sepsis and Hematogenous Septic Arthritis
2.5. Antibiotic Treatment in Mouse Model of S. aureus-Induced Sepsis
2.6. Mouse Model of Local S. aureus Septic Arthritis
2.7. Quantification of S. aureus Infection Using In Vivo Imaging System
2.8. Homogenate Preparation and Quantification of Bacterial Load in Kidneys and Joints
2.9. Quantification of Immunomodulators Using Enzyme-Linked Immunosorbent Assay
2.10. Bone Erosion Estimation Using μCT
3. Results
3.1. In Vivo Imaging Systems Are Able to Follow the Progression and Severity of Sepsis in Mice
3.2. Kidney IVIS Signals Correlate Positively with Kidney Abscess Severity and Kidney CFU Counts
3.3. The Efficacy of Antibiotic Treatment against Infection Was Monitored Using the Imaging System
3.4. Assessing Clinical Arthritis Progression Using Imaging System
3.5. Imaging Technique Can Determine the Infectious Progression over Time in a Local Infection Model
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Parameters | NMRI | C57BL/6 | ||||||
---|---|---|---|---|---|---|---|---|
Kidney | Whole Mice | Kidney | Whole Mice | |||||
ROI | ROI | ROI | ROI | |||||
p | r | p | r | p | r | p | r | |
Weight loss | ns | - | 0.01 | 0.3 | ns | - | ns | - |
Abscess score | 0.03 | 0.18 | ns | - | 0.0001 | 0.6 | ns | - |
KidneyCount | 0.008 | 0.2 | ns | - | 0.0001 | 0.6 | ns | - |
IL6 | ns | - | ns | - | ns | - | ns | - |
S100A8/A9 | 0.06 | 0.2 | 0.08 | 0.2 | ns | - | ns | - |
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Deshmukh, M.; Hu, Z.; Mohammad, M.; Jin, T. Utilization of In Vivo Imaging System to Study Staphylococcal Sepsis and Septic Arthritis Progression in Mouse Model. Pathogens 2024, 13, 652. https://doi.org/10.3390/pathogens13080652
Deshmukh M, Hu Z, Mohammad M, Jin T. Utilization of In Vivo Imaging System to Study Staphylococcal Sepsis and Septic Arthritis Progression in Mouse Model. Pathogens. 2024; 13(8):652. https://doi.org/10.3390/pathogens13080652
Chicago/Turabian StyleDeshmukh, Meghshree, Zhicheng Hu, Majd Mohammad, and Tao Jin. 2024. "Utilization of In Vivo Imaging System to Study Staphylococcal Sepsis and Septic Arthritis Progression in Mouse Model" Pathogens 13, no. 8: 652. https://doi.org/10.3390/pathogens13080652