Green Tea Catechin Epigallocatechin Gallate Inhibits Vegetative Cell Outgrowth and Expression of Beta-Lactamase Genes in Penicillin-Resistant Bacillus anthracis Strain PCr
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains and Media
2.2. Effect of EGCg on B. anthracis Spores
2.3. Time-Kill Kinetics Assay and MIC Determination
2.4. Beta-Lactamase Production and Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) Analysis
2.5. Statistical Analyses
3. Results
3.1. EGCg Decreased the MIC of Antibiotic Treatments of Penicillin-Resistant B. anthracis Strain PCr
3.2. Inhibitory Effect of EGCg on B. anthracis Vegetative Cell Outgrowth
3.3. Beta-Lactamase Production in Penicillin-Resistant B. anthracis Strain PCr
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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EGCg (µg/mL) | MIC of Penicillin (µg/mL) | MIC of Meropenem (µg/mL) | ||
---|---|---|---|---|
PCr | BA103 | PCr | BA103 | |
0 | >800 | 0.125 | >1024 | 0.063 |
25 | >800 | 0.25 | 256 | 0.063 |
50 | 100 | 0.016 | 128 | 0.031 |
100 | <100 | * | <64 | * |
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Okutani, A.; Morikawa, S.; Maeda, K. Green Tea Catechin Epigallocatechin Gallate Inhibits Vegetative Cell Outgrowth and Expression of Beta-Lactamase Genes in Penicillin-Resistant Bacillus anthracis Strain PCr. Pathogens 2024, 13, 699. https://doi.org/10.3390/pathogens13080699
Okutani A, Morikawa S, Maeda K. Green Tea Catechin Epigallocatechin Gallate Inhibits Vegetative Cell Outgrowth and Expression of Beta-Lactamase Genes in Penicillin-Resistant Bacillus anthracis Strain PCr. Pathogens. 2024; 13(8):699. https://doi.org/10.3390/pathogens13080699
Chicago/Turabian StyleOkutani, Akiko, Shigeru Morikawa, and Ken Maeda. 2024. "Green Tea Catechin Epigallocatechin Gallate Inhibits Vegetative Cell Outgrowth and Expression of Beta-Lactamase Genes in Penicillin-Resistant Bacillus anthracis Strain PCr" Pathogens 13, no. 8: 699. https://doi.org/10.3390/pathogens13080699