Differential Drug Susceptibility across Trichomonasvirus Species Allows for Generation of Varied Isogenic Clones of Trichomonas vaginalis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Tvag Isolates and Cell Culture
2.2. RNA Extraction, cDNA Synthesis, and qPCR
2.3. Drug Treatment Assays
2.4. Generation of Isogenic Clones
3. Results
3.1. Treatment with 2CMC Is Effective at Reducing RNA Expression by Strains of All Five TVV Species, but to Varying Degrees
3.2. Susceptibility to Clearance by 2CMC Varies by TVV Species
3.3. Isogenic Clones Generated after 8 Days of 2CMC Treatment
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Tvag Isolate | TVV1 | TVV2 | TVV3 | TVV4 | TVV5 |
---|---|---|---|---|---|
JH37A#2-B1 | + | - | - | - | - |
JH191A#4-B1 | + | - | - | - | - |
JH32A#4-A1 | - | - | + | - | - |
JH162A#4-A1 | + | + | + | - | + |
RU357-B1 | + | + | - | + | + |
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Hetzel, C.A.; Appah-Sampong, A.A.; Hurst-Manny, A.R.; Nibert, M.L. Differential Drug Susceptibility across Trichomonasvirus Species Allows for Generation of Varied Isogenic Clones of Trichomonas vaginalis. Pathogens 2024, 13, 733. https://doi.org/10.3390/pathogens13090733
Hetzel CA, Appah-Sampong AA, Hurst-Manny AR, Nibert ML. Differential Drug Susceptibility across Trichomonasvirus Species Allows for Generation of Varied Isogenic Clones of Trichomonas vaginalis. Pathogens. 2024; 13(9):733. https://doi.org/10.3390/pathogens13090733
Chicago/Turabian StyleHetzel, Carrie A., Akua A. Appah-Sampong, Austin R. Hurst-Manny, and Max L. Nibert. 2024. "Differential Drug Susceptibility across Trichomonasvirus Species Allows for Generation of Varied Isogenic Clones of Trichomonas vaginalis" Pathogens 13, no. 9: 733. https://doi.org/10.3390/pathogens13090733