Next Article in Journal
Betanodavirus and VER Disease: A 30-year Research Review
Next Article in Special Issue
Legionella pneumophila and Protozoan Hosts: Implications for the Control of Hospital and Potable Water Systems
Previous Article in Journal
Epstein–Barr Virus and Helicobacter Pylori Co-Infection in Non-Malignant Gastroduodenal Disorders
Previous Article in Special Issue
Water Quality as a Predictor of Legionella Positivity of Building Water Systems
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Pathogens
Volume 9
Issue 2
10.3390/pathogens9020105
pathogens-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Intracellular Behaviour of Three Legionella pneumophila Strains within Three Amoeba Strains, Including Willaertia magna C2c Maky

by
Issam Hasni
1,2,
Antoine Jarry
2,
Benjamin Quelard
2,
Antoine Carlino
2,
Jean-Baptiste Eberst
2,
Olivier Abbe
2 and
Sandrine Demanèche
2,*
1
Microbes, Evolution, Phylogeny and Infection Department, Institut de Recherche pour le Développement IRD 198, Institut Hospitalo-Universitaire (IHU), Aix-Marseille Université, 13007 Marseille, France
2
R&D Department, Amoéba, 38 Avenue des Frères Montgolfier, 69680 Chassieu, France
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(2), 105; https://doi.org/10.3390/pathogens9020105
Submission received: 10 January 2020 / Revised: 4 February 2020 / Accepted: 5 February 2020 / Published: 6 February 2020
(This article belongs to the Special Issue Legionella Contamination in Water Environment)
Browse Figures
Versions Notes

Abstract

Legionella pneumophila is a facultative intracellular pathogen found in aquatic environments as planktonic cells within biofilms and as intracellular parasites of free-living amoebae such as Acanthamoeba castellanii. This pathogen bypasses the elimination mechanism to replicate within amoebae; however, not all amoeba species support the growth of L. pneumophila. Willaertia magna C2c Maky, a non-pathogenic amoeba, was previously demonstrated to possess the ability to eliminate the L. pneumophila strain Paris. Here, we study the intracellular behaviour of three L. pneumophila strains (Paris, Philadelphia, and Lens) within W. magna C2c Maky and compare this strain to A. castellanii and W. magna Z503, which are used as controls. We observe the intracellular growth of strain Lens within W. magna Z503 and A. castellanii at 22 °C and 37 °C. Strain Paris grows within A. castellanii at any temperature, while it only grows at 22 °C within W. magna Z503. Strain Philadelphia proliferates only within A. castellanii at 37 °C. Within W. magna C2c Maky, none of the three legionella strains exhibit intracellular growth. Additionally, the ability of W. magna C2c Maky to decrease the number of internalized L. pneumophila is confirmed. These results support the idea that W. magna C2c Maky possesses unique behaviour in regard to L. pneumophila strains.
Keywords: free-living amoebae; Legionella; biological biocide; cooling towers free-living amoebae; Legionella; biological biocide; cooling towers

Share and Cite

MDPI and ACS Style

Hasni, I.; Jarry, A.; Quelard, B.; Carlino, A.; Eberst, J.-B.; Abbe, O.; Demanèche, S. Intracellular Behaviour of Three Legionella pneumophila Strains within Three Amoeba Strains, Including Willaertia magna C2c Maky. Pathogens 2020, 9, 105. https://doi.org/10.3390/pathogens9020105

AMA Style

Hasni I, Jarry A, Quelard B, Carlino A, Eberst J-B, Abbe O, Demanèche S. Intracellular Behaviour of Three Legionella pneumophila Strains within Three Amoeba Strains, Including Willaertia magna C2c Maky. Pathogens. 2020; 9(2):105. https://doi.org/10.3390/pathogens9020105

Chicago/Turabian Style

Hasni, Issam, Antoine Jarry, Benjamin Quelard, Antoine Carlino, Jean-Baptiste Eberst, Olivier Abbe, and Sandrine Demanèche. 2020. "Intracellular Behaviour of Three Legionella pneumophila Strains within Three Amoeba Strains, Including Willaertia magna C2c Maky" Pathogens 9, no. 2: 105. https://doi.org/10.3390/pathogens9020105

APA Style

Hasni, I., Jarry, A., Quelard, B., Carlino, A., Eberst, J.-B., Abbe, O., & Demanèche, S. (2020). Intracellular Behaviour of Three Legionella pneumophila Strains within Three Amoeba Strains, Including Willaertia magna C2c Maky. Pathogens, 9(2), 105. https://doi.org/10.3390/pathogens9020105

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop