Molecular and Phylogenetic Analysis of Tick-Borne Pathogens in Ticks Parasitizing Native Korean Goats (Capra hircus coreanae) in South Korea
Abstract
:1. Introduction
2. Results
2.1. Identification of Ticks
2.2. Identification of TBPs
2.3. Molecular and Phylogenetic Analyses
3. Discussion
4. Materials and Methods
4.1. Tick Collection and Species Identification
4.2. Molecular Detection of Ticks and TBPs
4.3. DNA Cloning
4.4. Nucleotide Sequencing and Phylogeny
Supplementary Material
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Species | Stage | Tick Pool (n) | A. bovis (16S) | T. luwenshuni (18S) | Coxiella-like Endosymbiont (16S) | Candidatus R. Longicornii (16S) | ||||
---|---|---|---|---|---|---|---|---|---|---|
Positive (%) | 95% CI | Positive (%) | 95% CI | Positive (%) | 95% CI | Positive (%) | 95% CI | |||
Haemaphysalis longicornis | Nymph | 15 | 1 (6.7) | 0–19.3 | 1 (6.7) | 0–19.3 | 1 (6.7) | 0–19.3 | 8 (53.3) | 28.1–78.6 |
Adult | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 10 (100) | 100 | |
Haemaphysalis flava | Nymph | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Adult | 4 | 0 | 0 | 1 (25.0) | 0–67.4 | 1 (25.0) | 0–67.4 | 0 | 0 | |
Ixodes nipponensis | Nymph | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Adult | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
Total | 40 | 1 (2.5) | 0–7.3 | 2 (5.0) | 0–11.8 | 2 (5.0) | 0–11.8 | 18 (45.0) | 29.6–60.4 |
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Seo, M.-G.; Kwon, O.-D.; Kwak, D. Molecular and Phylogenetic Analysis of Tick-Borne Pathogens in Ticks Parasitizing Native Korean Goats (Capra hircus coreanae) in South Korea. Pathogens 2020, 9, 71. https://doi.org/10.3390/pathogens9020071
Seo M-G, Kwon O-D, Kwak D. Molecular and Phylogenetic Analysis of Tick-Borne Pathogens in Ticks Parasitizing Native Korean Goats (Capra hircus coreanae) in South Korea. Pathogens. 2020; 9(2):71. https://doi.org/10.3390/pathogens9020071
Chicago/Turabian StyleSeo, Min-Goo, Oh-Deog Kwon, and Dongmi Kwak. 2020. "Molecular and Phylogenetic Analysis of Tick-Borne Pathogens in Ticks Parasitizing Native Korean Goats (Capra hircus coreanae) in South Korea" Pathogens 9, no. 2: 71. https://doi.org/10.3390/pathogens9020071