It Works! Organic-Waste-Assisted Trichoderma spp. Solid-State Fermentation on Agricultural Digestate
Round 1
Reviewer 1 Report
Overall, this manuscript is relatively well prepared. Some suggestions or comments are provided for authors' consideration.
- The Conclusion part can be improved to make it more attractive.
- Please provide the standard deviations for data in Table 1 and 2.
- Why we need to use Solid-State Fermentation (SSF)? Please provide some reasons for this point in Introduction section.
- The key issue that the authors are trying to solve can be more clearly pointed out in Introduced. The current presentation is basically acceptable, but not very attractive.
- Please pay attention to the grammar mistakes and italic format of microbial names (e.g., Line 287-288). Please check the entire manuscript.
- In the manuscript title, the authors are suggested to specify the type of food waste and the type of digestate. That would be more instructive.
- Can the authors provide a specific and whole name for Trichoderma spp.?
Author Response
Dear reviewer,
please see the attachment
Author Response File: Author Response.pdf
Reviewer 2 Report
Overall, the manuscript is good. However, I have some technical comments that need to be addressed before acceptance.
- Although the authors mention the objective of the research is valorization but it is not clear what specifically is valorization? Is it better root length as shown in figure 4 or the change in parameters shown in Table 3 or fungal growth? The data shown in figure 4 does not show a linear increase or decrease in root length indicating that some other unknown parameters might be involved.
- The difference in fungal biomass needs to be logically addressed. I recommend the authors test if the growth rate of T-22 strain is slower than others in the standard medium? Based on this, it appears that the phrase It works! in the title is too much extrapolation.
- The scientific names of the microorganisms should be italicized.
- Figure 3 forms the basis of quantification and it is not clear how many technical/biological replicates were used for analysis. It was not understandable how the primer efficiency was found to be 90%? Perhaps, it is a European format, but I would appreciate it if the authors can present the period as . not a comma in the figure. The same goes for figure 4.
Author Response
Dear reviewer,
please see the attachment
Author Response File: Author Response.pdf
Round 2
Reviewer 2 Report
The authors did a great job revising.