Partial Replacement of Oat Hay with Whole-Plant Hydroponic Barley Seedlings Modulates Ruminal Microbiota and Affects Growth Performance of Holstein Heifers
Abstract
:1. Introduction
2. Materials and Methods
2.1. Hydroponic Barley Seedling Production
2.2. Nutritional Compositions of Hydroponic Barley Seedlings
2.3. Animals and Experimental Design
2.4. Sample Collection and Analysis
2.5. Statistical Analysis
3. Results
3.1. Feed intake and Growth Performance
3.2. Nutrient Digestibility and Nitrogen Recycling
3.3. Microbial Population and Its Association with Nutrient Digestibility
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Items | Treatment | ||
---|---|---|---|
CON | 25% HBS | 50% HBS | |
Ingredients, % DM | |||
Corn | 2.59 | 2.59 | 2.59 |
Soybean meal | 12.58 | 12.58 | 12.58 |
Wheat | 2.96 | 2.96 | 2.96 |
DDGS 1 | 11.1 | 11.1 | 11.1 |
Wheat bran | 5.55 | 5.55 | 5.55 |
Corn silage | 20 | 20 | 20 |
Wheat stalk | 27 | 27 | 27 |
Hydroponic barley seedlings | 0 | 4 | 8 |
Oat hay | 16 | 12 | 8 |
Calcium bicarbonate | 0.37 | 0.37 | 0.37 |
Salt | 0.37 | 0.37 | 0.37 |
Limestone powder | 0.74 | 0.74 | 0.74 |
2% Premix 2 | 0.74 | 0.74 | 0.74 |
Total | 100 | 100 | 100 |
Nutrient composition | |||
DM, % | 47.27 | 45.95 | 45.06 |
CP, %DM | 13.51 | 13.87 | 14.02 |
EE, %DM | 2.13 | 2.25 | 2.41 |
NDF, %DM | 45.99 | 43.97 | 43.48 |
ADF, %DM | 27.26 | 25.30 | 25.13 |
Ash, %DM | 13.09 | 13.40 | 14.11 |
NEL 3, MJ/kg DM | 3.98 | 4.10 | 4.08 |
Items | Treatment | SEM | p-Value | ||||
---|---|---|---|---|---|---|---|
CON | 25% HBS | 50% HBS | Treat | Week | Treat × Week | ||
Dry matter intake, kg/d | 7.99 a | 7.90 ab | 7.74 b | 0.06 | 0.04 | <0.01 | <0.01 |
Body height | 120.43 | 121.72 | 121.26 | 0.50 | 0.17 | <0.01 | 0.36 |
Body oblique length | 129.93 | 131.80 | 132.96 | 0.91 | 0.12 | <0.01 | 0.09 |
Chest circumference | 155.49 | 157.22 | 156.72 | 0.50 | 0.05 | <0.01 | 0.73 |
Body weight | 333.12 a | 335.14 a | 326.88 b | 1.70 | <0.01 | <0.01 | 0.22 |
Items | Treatment | SEM | p-Value | ||
---|---|---|---|---|---|
CON | 25% HBS | 50% HBS | |||
CP | 62.81 a | 60.26 a | 54.84 b | 0.77 | <0.01 |
EE | 75.20 | 72.19 | 71.41 | 0.94 | 0.22 |
NDF | 51.95 a | 44.22 b | 36.84 c | 1.34 | <0.01 |
ADF | 49.74 a | 40.62 b | 32.58 c | 1.43 | <0.01 |
OM | 63.64 a | 60.60 a | 56.19 b | 0.78 | <0.01 |
Items | Treatment | SEM | p-Value | ||
---|---|---|---|---|---|
CON | 25% HBS | 50% HBS | |||
N intake, g/d | 172.80 | 175.36 | 173.75 | 0.78 | 0.42 |
Urine volume, L/d | 15.34 | 15.66 | 18.16 | 0.57 | 0.09 |
N output, g/d | |||||
Feces | 63.72 c | 68.99 b | 78.15 a | 1.35 | <0.01 |
Urine | 60.42 | 59.29 | 58.65 | 1.39 | 0.88 |
Retention 1 | 48.66 a | 47.08 a | 36.94 b | 1.73 | 0.01 |
N output, % of N intake | |||||
Feces | 37.17 b | 39.78b | 45.16 a | 0.77 | <0.01 |
Urine | 34.84 | 33.64 | 33.74 | 0.81 | 0.81 |
Retention | 27.99 a | 26.58 a | 21.10 b | 1.01 | 0.01 |
Items | Treatment | SEM | p-Value | ||
---|---|---|---|---|---|
CON | 25% HBS | 50% HBS | |||
Lachnospiraceae_NK3A20_group | 0.86 b | 1.09 ab | 1.58 a | 0.12 | 0.02 |
Lachnospiraceae_XPB1014_group | 0.73 b | 1.09 a | 1.25 a | 0.08 | 0.01 |
norank_f_Bacteroidales_RF16_group | 1.24 a | 0.79 b | 0.75 b | 0.09 | 0.03 |
unclassified_f_Lachnospiraceae | 0.83 b | 0.81 b | 1.12 a | 0.06 | 0.04 |
SP3-e08 | 0.40 a | 0.71 a | 0.22 b | 0.06 | <0.01 |
unclassified_f_Rikenellaceae | 0.38 a | 0.31 a | 0.14 b | 0.04 | <0.01 |
norank_f_norank_o_WCHB1-41 | 0.35 a | 0.24 ab | 0.17 b | 0.03 | 0.03 |
Sphaerochaeta | 0.24 a | 0.20 ab | 0.09 b | 0.02 | <0.01 |
Ruminiclostridium | 0.14 a | 0.13 a | 0.08 b | 0.01 | <0.01 |
Lachnospiraceae_FCS020_group | 0.05 ab | 0.04 b | 0.08 a | 0.01 | 0.04 |
unclassified_o_Oscillospirales | 0.06 a | 0.04 ab | 0.03 b | <0.01 | 0.03 |
Colidextribacter | 0.01 b | 0.04 a | 0.08 a | 0.01 | 0.02 |
Bacillus | 0.02 b | 0.04 b | 0.06 a | 0.01 | <0.01 |
norank_f_norank_o_LD1-PB3 | 0.01 b | 0.04 a | 0.02 ab | 0.01 | 0.03 |
Agathobacter | 0.01 b | 0.02 ab | 0.02 a | <0.01 | 0.03 |
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Ren, P.; Deng, M.; Feng, J.; Li, R.; Ma, X.; Liu, J.; Wang, D. Partial Replacement of Oat Hay with Whole-Plant Hydroponic Barley Seedlings Modulates Ruminal Microbiota and Affects Growth Performance of Holstein Heifers. Microorganisms 2022, 10, 2000. https://doi.org/10.3390/microorganisms10102000
Ren P, Deng M, Feng J, Li R, Ma X, Liu J, Wang D. Partial Replacement of Oat Hay with Whole-Plant Hydroponic Barley Seedlings Modulates Ruminal Microbiota and Affects Growth Performance of Holstein Heifers. Microorganisms. 2022; 10(10):2000. https://doi.org/10.3390/microorganisms10102000
Chicago/Turabian StyleRen, Peng, Mengmeng Deng, Juan Feng, Ruocheng Li, Xiaojiao Ma, Jianxin Liu, and Diming Wang. 2022. "Partial Replacement of Oat Hay with Whole-Plant Hydroponic Barley Seedlings Modulates Ruminal Microbiota and Affects Growth Performance of Holstein Heifers" Microorganisms 10, no. 10: 2000. https://doi.org/10.3390/microorganisms10102000