Effect of Iron Limitation, Elevated Temperature, and Florfenicol on the Proteome and Vesiculation of the Fish Pathogen Aeromonas salmonicida
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strain, Cultivation, Stress Conditions, and Harvest
2.2. Preparation of Cytosolic, Inner Membrane, and Outer Membrane Protein Fractions
2.3. Preparation of OMV Protein Fraction
2.4. OMV Nanoparticle-Tracking Analysis
2.5. S-Trap Protein Digestion and Peptide Fractionation
2.6. Preparation of the Extracellular Protein Fraction and In-Gel Digestion
2.7. Bioinformatic Tools
2.7.1. PSORTb
2.7.2. SignalP
2.7.3. eggNOG
2.8. Mass Spectrometry Data Acquisition and Analysis
2.9. Transmission Electron Microscopy
2.10. Field Emission Scanning Electron Microscopy
3. Results
3.1. Evaluation of the Subcellular Fractionation
3.2. Influence of the Stress Conditions on the Composition of the Subproteomes of A. salmonicida
3.3. The Outer Membrane Vesicles of A. salmonicida
4. Discussion
4.1. Subcellular Fractionation
4.2. Response to the Iron Limitation
4.3. Response to an Elevated Incubation Temperature
4.4. Response to Florfenicol
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Iron Limitation | Temperature | Antibiotics | |
---|---|---|---|
Excerpt of the proteomics results | Several upregulated IROMPs (TonB-dependent proteins, iron/siderophore transporters, hemolysin, aerolysin) | Upregulated hemolysin, putative hemin receptor, oxidative-stress chaperon, pullulanase, uncharacterized protein | Increased ribosomal proteins in extracellular space and outer membrane vesicles |
Vesiculation (Mean particle size of control: 172 nm) | Slightly increased vesicle size (Mean particle size: 195 nm) | Slightly decreased vesicle size (Mean particle size: 151 nm) | Decreased vesicle size (Mean particle size: 102 nm) potentially due to prophage induction |
Vesiculation amount | Comparable vesiculation amount to control condition | Less vesiculation compared to control | Comparable vesiculation amount to control condition |
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Kroniger, T.; Mehanny, M.; Schlüter, R.; Trautwein-Schult, A.; Köllner, B.; Becher, D. Effect of Iron Limitation, Elevated Temperature, and Florfenicol on the Proteome and Vesiculation of the Fish Pathogen Aeromonas salmonicida. Microorganisms 2022, 10, 1735. https://doi.org/10.3390/microorganisms10091735
Kroniger T, Mehanny M, Schlüter R, Trautwein-Schult A, Köllner B, Becher D. Effect of Iron Limitation, Elevated Temperature, and Florfenicol on the Proteome and Vesiculation of the Fish Pathogen Aeromonas salmonicida. Microorganisms. 2022; 10(9):1735. https://doi.org/10.3390/microorganisms10091735
Chicago/Turabian StyleKroniger, Tobias, Mina Mehanny, Rabea Schlüter, Anke Trautwein-Schult, Bernd Köllner, and Dörte Becher. 2022. "Effect of Iron Limitation, Elevated Temperature, and Florfenicol on the Proteome and Vesiculation of the Fish Pathogen Aeromonas salmonicida" Microorganisms 10, no. 9: 1735. https://doi.org/10.3390/microorganisms10091735