Metagenomics Reveal Microbial Effects of Lotus Root–Fish Co-Culture on Nitrogen Cycling in Aquaculture Pond Sediments
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experiment Design
2.2. Sample Sampling and Pre-Treatment
2.3. Physicochemical Analysis
2.4. DNA Extraction and Metagenomic Sequencing
2.5. Statistical Analysis
3. Results
3.1. Sediment Physicochemical Properties
3.2. Microbial Community Structure
3.3. Nitrogen Cycling Enzyme Family
3.4. Nitrogen Cycle Pathways and Functional Gene Family
3.5. Gene Abundance of Nitrogen Cycle Microorganisms
3.6. Differences in Microbial Abundance Involved in Nitrogen Cycle
4. Discussion
4.1. Differences of Nutrient Content and Microbial Abundance in Sediments before and after Culture in Different Culture Models
4.2. Differences in Nitrogen Cycle-Related Enzymes in Sediments before and after Culture in Different Culture Models
4.3. Differences in Nitrogen Cycle-Related Metabolic Pathways in the Sediments before and after Culture in Different Culture Models
4.4. Differences in Nitrogen Cycle-Related Microorganism in the Sediments before and after Culture in Different Culture Models
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample | NH4+ (mg kg−1) | NO3− (mg kg−1) | NO2− (mg kg−1) | TN (g kg−1) | DP (mg kg−1) | TP (g kg−1) | OM (g kg−1) | pH |
---|---|---|---|---|---|---|---|---|
FOA | 147.07 ± 13.35 b | 13.27 ± 2.76 a | 1.31 ± 0.19 a | 2.28 ± 0.06 a | 36.47 ± 2.61 a | 1.38 ± 0.07 a | 70.94 ± 0.94 a | 7.88 ± 0.03 a |
FPA | 67.48 ± 7.07 c | 11.04 ± 0.70 a | 0.62 ± 0.09 b | 2.09 ± 0.08 b | 38.67 ± 6.70 a | 1.23 ± 0.07 b | 64.99 ± 1.39 b | 7.81 ± 0.06 a |
FOB | 176.34 ± 7.89 a | 12.47 ± 2.98 a | 0.65 ± 0.07 b | 2.12 ± 0.05 b | 17.08 ± 1.97 b | 1.43 ± 0.03 a | 61.73 ± 0.64 c | 7.66 ± 0.06 b |
FPB | 167.98 ± 3.76 a | 9.62 ± 2.83 a | 0.60 ± 0.07 b | 2.16 ± 0.05 b | 14.22 ± 1.66 b | 1.36 ± 0.06 a | 61.31 ± 1.66 c | 7.64 ± 0.05 b |
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Yang, Z.; Yao, Y.; Sun, M.; Li, G.; Zhu, J. Metagenomics Reveal Microbial Effects of Lotus Root–Fish Co-Culture on Nitrogen Cycling in Aquaculture Pond Sediments. Microorganisms 2022, 10, 1740. https://doi.org/10.3390/microorganisms10091740
Yang Z, Yao Y, Sun M, Li G, Zhu J. Metagenomics Reveal Microbial Effects of Lotus Root–Fish Co-Culture on Nitrogen Cycling in Aquaculture Pond Sediments. Microorganisms. 2022; 10(9):1740. https://doi.org/10.3390/microorganisms10091740
Chicago/Turabian StyleYang, Zhen, Yanhong Yao, Meng Sun, Gu Li, and Jianqiang Zhu. 2022. "Metagenomics Reveal Microbial Effects of Lotus Root–Fish Co-Culture on Nitrogen Cycling in Aquaculture Pond Sediments" Microorganisms 10, no. 9: 1740. https://doi.org/10.3390/microorganisms10091740