Exploring Microeukaryote Community Characteristics and Niche Differentiation in Arid Farmland Soil at the Northeastern Edge of the Tibetan Plateau
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sampling Areas and Site Descriptions
2.2. Soil Sample Collection and Storage
2.3. DNA Extraction and PCR Amplifications
2.4. High-Throughput Sequencing
2.5. Data Processing
2.6. Statistical Analysis
3. Results
3.1. Taxonomic Compositions of the Soil Microeukaryote Communities in Different Sampling Areas
3.2. Alpha Diversity of the Soil Microeukaryote Communities
3.3. Beta Diversity of the Soil Microeukaryote Communities
3.4. Co-Occurrence Networks and Assembly Mechanisms of Soil Microeukaryote Communities
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Area Number | Sampling Area Short Name | Climate Type | Crop Type | Longitude (E) | Latitude (N) | Average Soil Temperature (°C) |
---|---|---|---|---|---|---|
#1 | Longxi | Temperate monsoon | Bupleurum chinense | 104°41′20″ | 34°58′16″ | 29.67 |
Phaseolus vulgaris | 104°41′20″ | 34°58′16″ | 24.33 | |||
Solanum tuberosum | 104°41′20″ | 34°58′16″ | 27.33 | |||
Allium.fistulosum | 104°41′21″ | 34°58′16″ | 28.67 | |||
Zea mays | 104°41′21″ | 34°58′16″ | 28.33 | |||
Glycine max | 104°41′19″ | 34°58′16″ | 35.00 | |||
Allium tuberosum | 104°41′21″ | 34°58′15″ | 31.00 | |||
#2 | Dingxi | Temperate continental | Solanum tuberosum | 104°30′32″ | 35°27′04″ | 30.00 |
Brassica oleracea | 104°30′38″ | 35°27′01″ | 28.00 | |||
Libanotis seseloides | 104°30′47″ | 35°26′59″ | 29.00 | |||
#3 | Huining | Temperate monsoon | Zea mays | 105°02′35″ | 35°39′05″ | 34.33 |
Sesamum indicum | 105°02′35″ | 35°39′05″ | 31.33 | |||
#4 | Linxia | Temperate semi-arid and semi-humid | Medicago sativa | 103°16′01″ | 35°32′52″ | 23.33 |
Lilium brownii | 103°16′19″ | 35°32′46″ | 30.83 | |||
#5 | Tanchang | Temperate continental | Zea mays | 104°20′34″ | 34°02′02″ | 25.00 |
Phaseolus vulgaris | 104°20′33″ | 34°01′59″ | 25.00 | |||
Cucumis sativus | 104°20′32″ | 34°01′57″ | 23.33 | |||
Cucurbita moschata | 104°20′32″ | 34°01′59″ | 24.00 | |||
Vigna unguiculata | 104°20′32″ | 34°01′59″ | 23.67 | |||
Raphanus sativus | 104°20′31″ | 34°01′59″ | 24.33 | |||
Capsicum annuum | 104°20′31″ | 34°01′59″ | 25.00 | |||
Solanum tuberosum | 104°20′33″ | 34°02′01″ | 27.67 | |||
#6 | Wudu | Subtropical semi-humid | Vigna unguiculata | 105°04′46″ | 33°19′42″ | 30.33 |
Zea mays | 105°04′46″ | 33°19′41″ | 30.33 | |||
#7 | Dayugou | Plateau continental | Brassica napusL | 103°34′47″ | 34°25′52″ | 24.00 |
Hordeum vulgare | 103°34′48″ | 34°25′53″ | 25.33 | |||
Vicia faba | 103°34′49″ | 34°25′55″ | 22.33 | |||
Pisum sativum | 103°34′48″ | 34°25′50″ | 21.33 | |||
Angelica dahurica | 103°34′48″ | 34°25′55″ | 25.67 | |||
Solanum tuberosum | 103°34′48″ | 34°25′56″ | 25.00 | |||
Triticum aestivum | 103°34′4″ | 34°25′58″ | 26.67 |
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Chen, L.; Han, H.; Wang, C.; Warren, A.; Ning, Y. Exploring Microeukaryote Community Characteristics and Niche Differentiation in Arid Farmland Soil at the Northeastern Edge of the Tibetan Plateau. Microorganisms 2023, 11, 2510. https://doi.org/10.3390/microorganisms11102510
Chen L, Han H, Wang C, Warren A, Ning Y. Exploring Microeukaryote Community Characteristics and Niche Differentiation in Arid Farmland Soil at the Northeastern Edge of the Tibetan Plateau. Microorganisms. 2023; 11(10):2510. https://doi.org/10.3390/microorganisms11102510
Chicago/Turabian StyleChen, Lingyun, Haifeng Han, Chunhui Wang, Alan Warren, and Yingzhi Ning. 2023. "Exploring Microeukaryote Community Characteristics and Niche Differentiation in Arid Farmland Soil at the Northeastern Edge of the Tibetan Plateau" Microorganisms 11, no. 10: 2510. https://doi.org/10.3390/microorganisms11102510