Bacillus velezensis BV01 Has Broad-Spectrum Biocontrol Potential and the Ability to Promote Plant Growth
Abstract
:1. Introduction
2. Materials and Methods
2.1. Tested Strains
2.2. Evaluation of Antagonistic Abilities In Vitro
2.3. Assessment of Antifungal Activity of Bacterial Sterile Filtrate
2.4. Morphological Observation and Molecular Identification of Strain BV01
2.5. Determination of Enzyme Activity and Secondary Metabolites
2.6. Biocontrol Activity of Strain BV01 In Vivo
2.7. Plant-Growth-Promoting Assays in a Greenhouse
2.8. Statistical Analysis
3. Results
3.1. Inhibitory Effects of Four Tested Bacterial Strains against Eight Fungal Phytopathogens
3.2. Identification of Strain BV01
3.3. Detection of Antagonism-Related Lytic Enzymes
3.4. Biocontrol Effects of Bacterial Strains BV01 and JDF on Wheat Root Rot
3.5. Biocontrol Effect of Strain BV01 on Fusarium Wilt
3.6. Growth-Promotion Effects of Strain BV01 on Wheat and Pepper
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Strain | Characteristics Relevant to This Work | Source |
---|---|---|
Bacillus velezensis CGMCC 1.60184 | Isolated from the State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences (116.38982° E, 40.01076° N) on 6 November 2019 | This work |
B. velezensis JDF | Registration of broad-spectrum antagonism, stress resistance and growth-promoting ability, used as a positive control | Isolated from NongBaoShengWu® bacterial agent |
B. subtilis L01 | Registration of antimicrobial activity and strong stress resistance, used as a positive control | Isolated from LvLong® bacterial agent |
B. subtilis BS208 | Registration of prevention and control of gray mold and powdery mildew, used as a positive control | Isolated from GuanLan® bacterial agent |
Bipolaris sorokiniana PP12 | Causes wheat root rot | Provided by Prof. Niu Yongchun of Chinese Academy of Agricultural Sciences |
Botrytis cinerea PP1 | Causes tomato gray mold | Provided by Prof. Qiu Jiyan of Beijing Academy of Agricultural Sciences |
Colletotrichum capsici PP6 | Causes ring rot disease | |
Fusarium graminearum PP15 | Causes Fusarium head blight | Provided by Prof. Ma Zhengqiang of Nanjing Agricultural University |
F. oxysporum F6 | Causes blight disease | Provided by Prof. Li Qili of Guangxi Academy of Agricultural Sciences |
Neocosmospora rubicola PP23 | Causes root and stem rot | Provided by Dr. Fu Shenzhan of Institute of Microbiology, Chinese Academy of Sciences |
Rhizoctonia solani PP11 | Causes wilt disease | Provided by Prof. Niu Yongchun of Chinese Academy of Agricultural Sciences |
Verticillium dahliae PP8 | Causes greensickness |
Strains | Dual-Culture Inhibition Rate (%) | Fermentation Broth Inhibition Rate (%) | ||||||
---|---|---|---|---|---|---|---|---|
CGMCC 1.60184 | JDF | L01 | BS208 | CGMCC 1.60184 | JDF | L01 | BS208 | |
B. sorokiniana PP12 | 80.68 ± 1.23 a | 79.55 ± 0.93 a | 77.27 ± 1.05 b | 27.27 ± 2.47 c | 92.26 ± 0.68 a | 76.77 ± 0.23 b | 70.32 ± 1.03 c | 8.39 ± 1.89 d |
B. cinerea PP1 | 82.95 ± 1.23 b | 85.23 ± 2.23 a | 85.23 ± 2.14 a | 0.00 ± 0.83 c | 81.82 ± 0.82 a | 64.77 ± 1.00 c | 71.59 ± 0.58 b | 2.27 ± 1.44 d |
C. capsici PP6 | 79.55 ± 0.64 a | 75.00 ± 1.25 b | 77.27 ± 1.07 b | 26.14 ± 1.68 c | 72.73 ± 0.75 a | 71.59 ± 0.86 a | 72.73 ± 0.42 a | 4.55 ± 1.45 b |
F. graminearum PP15 | 61.36 ± 1.09 b | 65.91 ± 0.49 a | 62.50 ± 0.62 b | 0.00 ± 0.62 c | 59.09 ± 1.66 a | 43.18 ± 0.52 c | 47.53 ± 0.66 b | 13.64 ± 0.97 d |
F. oxysporum F6 | 65.91 ± 0.66 a | 62.50 ± 1.03 b | 52.27 ± 0.71 c | 32.95 ± 1.27 d | 56.79 ± 1.23 a | 13.58 ± 1.06 b | 12.04 ± 0.69 b | 8.95 ± 0.46 c |
N. rubicola PP23 | 56.52 ± 1.02 a | 50.72 ± 0.93 b | 50.72 ± 1.23 b | 42.03 ± 1.24 c | 36.13 ± 1.19 a | 12.18 ± 0.99 b | 12.18 ± 0.52 b | 4.19 ± 0.74 c |
R. solani PP11 | 69.32 ± 0.71 a | 54.55 ± 0.86 b | 53.41 ± 1.23 b | 0.00 ± 1.7 c | 46.59 ± 1.23 a | 0.00 ± 1.08 c | 0.00 ± 1.21 c | 5.68 ± 0.56 b |
V. dahliae PP8 | 70.15 ± 1.18 a | 58.21 ± 0.73 b | 55.22 ± 1.35 c | 32.84 ± 0.88 d | 71.72 ± 1.18 a | 59.07 ± 0.73 b | 61.03 ± 1.35 b | 33.95 ± 0.88 c |
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Huang, T.; Zhang, Y.; Yu, Z.; Zhuang, W.; Zeng, Z. Bacillus velezensis BV01 Has Broad-Spectrum Biocontrol Potential and the Ability to Promote Plant Growth. Microorganisms 2023, 11, 2627. https://doi.org/10.3390/microorganisms11112627
Huang T, Zhang Y, Yu Z, Zhuang W, Zeng Z. Bacillus velezensis BV01 Has Broad-Spectrum Biocontrol Potential and the Ability to Promote Plant Growth. Microorganisms. 2023; 11(11):2627. https://doi.org/10.3390/microorganisms11112627
Chicago/Turabian StyleHuang, Ting, Yi Zhang, Zhihe Yu, Wenying Zhuang, and Zhaoqing Zeng. 2023. "Bacillus velezensis BV01 Has Broad-Spectrum Biocontrol Potential and the Ability to Promote Plant Growth" Microorganisms 11, no. 11: 2627. https://doi.org/10.3390/microorganisms11112627