Cellulose Synthase in Atacama Cyanobacteria and Bioethanol Production from Their Exopolysaccharides
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cyanobacteria Culture Conditions
2.2. Extraction and Quantification of Genomic DNA
2.3. Molecular Study of Cellulose Synthase Gene
2.3.1. PCR Amplification, Sequencing, and Similarity Searches for Cellulose Synthase Gene
2.3.2. Analysis of the Conserved Motif D,D,D,35QXXRW
2.3.3. Phylogenetic Analyses
2.4. EPS Purification
2.5. Enzymatic EPS Digestion and Fermentation
2.6. Glucose and Ethanol Determination
2.7. EPS Surface Morphology and Chemical Composition
3. Results and Discussion
3.1. Molecular Analysis
3.1.1. Cellulose Synthase Gene Amplification
3.1.2. Cellulose Synthase Catalytic Domains Alignment
3.1.3. Alignment of the Catalytic Region of Cellulose Synthase from Strain LLA-10
3.1.4. Phylogenetic Analysis with the Catalytic Region of Cellulose Synthase of Cyanobacterium Strain LLA-10
3.2. Enzymatic EPS Degradation and Fermentation
3.2.1. EPS Content among Cyanobacteria Strains
3.2.2. Fermentation and Ethanol Production from Cyanobacteria EPS
3.2.3. Microscopy Analysis of Cyanobacteria EPS
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Strain | EPS (g L−1) |
---|---|
LLA-10 | 2.65 ± 0.13 |
LLC-10 | 7.11 ± 0.02 |
RPU-10 | 1.84 ± 0.05 |
LCHI-10 | 1.73 ± 0.23 |
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Galetović, A.; Peña, G.; Fernández, N.; Urrutia, M.; Flores, N.; Gómez-Silva, B.; Di Ruggiero, J.; Shene, C.; Bustamante, M. Cellulose Synthase in Atacama Cyanobacteria and Bioethanol Production from Their Exopolysaccharides. Microorganisms 2023, 11, 2668. https://doi.org/10.3390/microorganisms11112668
Galetović A, Peña G, Fernández N, Urrutia M, Flores N, Gómez-Silva B, Di Ruggiero J, Shene C, Bustamante M. Cellulose Synthase in Atacama Cyanobacteria and Bioethanol Production from Their Exopolysaccharides. Microorganisms. 2023; 11(11):2668. https://doi.org/10.3390/microorganisms11112668
Chicago/Turabian StyleGaletović, Alexandra, Gabriel Peña, Nicole Fernández, Milton Urrutia, Nataly Flores, Benito Gómez-Silva, Jocelyne Di Ruggiero, Carolina Shene, and Mariela Bustamante. 2023. "Cellulose Synthase in Atacama Cyanobacteria and Bioethanol Production from Their Exopolysaccharides" Microorganisms 11, no. 11: 2668. https://doi.org/10.3390/microorganisms11112668