Isolation of Bacillus subtilis and Bacillus pumilus with Anti-Vibrio parahaemolyticus Activity and Identification of the Anti-Vibrio parahaemolyticus Substance
Round 1
Reviewer 1 Report
This manuscript describes Bacillus strains isolated from aquaculture water, that showing antimicrobial activity to Vibrio parahaemolyticus.
This includes lots of data, and data can be useful for aquaculture application, but this requires some improvement for the publication.
in 2.2.1. Primary screening, please specify the Vibrio strain you used. Give more information. It this Vibrio virulent in aquaculture? and carry any virulence gene? If not, it is useless to inhibit non virulent Vibrio.
in 2.3. author used 16S rRNA sequencing, but this it not a ideal method for Bacillus identification. Please examine additional experiments for identification.
in section 2.4. Why did you use only 2 strain? Even B. HLJ1 Bacillus is not showing the strongest antimicrobial activity.
2.5.2. for spore production assay, I am not sure that 37C is good for sporulation.
Please describe relationship between Table 1 and Figure 1.
No comments
Author Response
Please see the attachment.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
The manuscript is well written and the data are well- presented. However, some points need to be improved such as:
1- The introduction should be supported with a paragraph about the economic losses associated with V. parahaemolyticus infection in aquaculture.
2- All the abbreviations should firstly mentioned in the full names, like MRS agar, MIC, CLSI, etc.
3- The result of sensitivity test of -V. parahaemolyticus to different isolate should be supported with photos, showing the inhibition zones.
4- The cost of using these probiotics commercial practices and also, their most effective dose should be mentioned in rhe conclusion section
The quality of English language is good, just minor check of the grammer of some sentences should be done.
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
Round 2
Reviewer 1 Report
No additional comment
No additional comment
Author Response
Thanks again for your review and good luck!
