Achieving Discharge Limits in Single-Stage Domestic Wastewater Treatment by Combining Urban Waste Sources and Phototrophic Mixed Cultures
Abstract
:1. Introduction
2. Materials and Methods
2.1. Source of Biowastes
2.2. Inoculum
2.3. Experimental Design
2.3.1. Specific Phototrophic Activity (SPA) Tests
2.3.2. Semicontinuous Photobioreactors
2.4. Analytical Methods
2.5. Microbial Community Analysis
3. Results and Discussion
3.1. Specific Phototrophic Assay (SPA)
3.2. Semicontinuous Operation Using CSTR to Evaluate the Anaerobic and Aerobic Conditions: ORP Influence on the Phototrophic Mixed Culture
3.3. Semicontinuous Operation Using MBR to Evaluate the SRT and HRT Parameters
4. Implications and Future Perspectives
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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SCOD (mg/L) | TCOD (mg/L) | NH4+ (mgN/L) | PO43− (mgP/L) | VSS (mg/L) * | |
---|---|---|---|---|---|
Campaign 1: Days 1–39 | |||||
OFMSW hydrolysate (n = 9) | 67,000 (1000) | 78,000 (3000) | 59 (1) | 75 (4) | 58 (1) |
DWW Estiviel (n = 18) | 196 (6) | 270 (8) | 48 (1) | 5 (1) | 26 (4) |
Campaign 2: Days 40–60 | |||||
OFMSW hydrolysate (n = 9) | 62,000 (2000) | 78,000 (3000) | 37 (2) | 132 (2) | 58 (1) |
DWW Estiviel (n = 18) | 168 (3) | 270 (8) | 54 (1) | 6 (1) | 24 (2) |
Campaign 3: Days 61–147 | |||||
OFMSW hydrolysate (n = 9) | 51,000 (1000) | 57,000 (300) | 85 (4) | 130 (20) | 53 (1) |
DWW Estiviel (n = 18) | 106 (3) | 500 (22) | 48 (2) | 5 (1) | 90 (15) |
SPA source biowastes | |||||
OFMSW hydrolysate (n = 9) | 67,000 (1000) | 78,000 (3000) | 59 (1) | 75 (4) | 58 (1) |
DWW La Gavia (n = 4) | 155 (10) | 203 (20) | 65 (1) | 16 (1) | 69 (10) |
Inoculum (SPA and semicontinuous photobioreactors) | |||||
Inoculum of PPB (n = 3) | 330 (10) | 800 (20) | 6 (1) | 5 (1) | 368 (3) |
COD:N Ratio | SCOD (mg/L) | NH4+ (mgN/L) | PO43 (mgP/L) | VSS (mg/L) | TOC (mg/L) | pH |
---|---|---|---|---|---|---|
100:3 | 2080 ± 90 | 62 ± 1 | 11.6 ± 0.6 | 92.0 ± 0.0 | 890.5 ± 59.4 | 6.92 ± 0.02 |
100:4 | 1400 ± 40 | 53 ±1 | 8.5 ± 0.4 | 100 ± 10 | 587.2 ± 39.1 | 6.91 ± 0.02 |
100:5 | 1080 ± 50 | 59 ± 2 | 8 ± 2 | 104 ± 5 | 554.8 ± 37.0 | 6.9 ± 0.02 |
100:6 | 1190 ± 50 | 53 ± 1 | 8.0 ± 0.7 | 92 ± 5 | 478.8 ± 31.9 | 7.03 ± 0.02 |
100:7 | 1010 ± 60 | 53 ± 3 | 7.2 ± 0.2 | 88 ± 5 | 401.3 ± 26.8 | 7.04 ± 0.02 |
DWW (control) | 400 ± 90 | 54.8 ± 0.6 | 5.0 ± 0.9 | 86 ± 8 | 54.4 ± 3.6 | 7.55 ± 0.02 |
LF (control) | 1600 ± 90 | 11.4 ± 0.6 | 4.7 ± 0.1 | 41 ± 2 | 716.8 ± 47.8 | 4.46 ± 0.02 |
CSTR Mode | MBR Mode | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Reactor | CSTR-1 and CSTR-2 | MBR-1 | MBR-2 | |||||||||||
Stage | I | II | III | IV | V-a | V-b | VI-a | VI-b | VI-c | VI-d | ||||
Period (d) | 01–15 | 15–40 | 40–62 | 62–96 | 96–118 | 118–145 | 96–111 | 111–118 | 118–125 | 125–145 | ||||
Time (d) | 15 | 25 | 22 | 34 | 22 | 27 | 15 | 7 | 7 | 20 | ||||
HRT (d) | 3 | 3 | 5 | 5 | 3.8 | 3.8 | 3 | 2.3 | 2.3 | 1.5 | ||||
SRT (d) | 3 | 3 | 5 | 5 | 5 | 5 | 4 | 3 | 3 | 2 | ||||
COD:N | 100:4.4 ± 1.7 | 100:6.6 ± 0.9 | 100:6.7 ± 0.7 | 100:5.8 ± 0.6 | 100:6.9 ± 0.6 | 100:5.8 ± 0.6 | 100:6.6 ± 0.8 | 100:6.9 ± 0.7 | 100:6.2 ± 1.4 | 100:5.4 ± 0.6 | ||||
OLR (g COD/L·d) | 0.41 (0.05) | 0.30 (0.07) | 0.21 (0.04) | 0.21 (0.05) | 0.23 (0.03) | 0.30 (0.04) | 0.29 (0.05) | 0.39 (0.02) | 0.40 (0.01) | 0.76 (0.09) | ||||
SLR (g COD/gVSS·d) * | 1.6 (0.6) | 1.6 (0.7) | 0.8 (0.3) | 1.0 (0.3) | 0.40 (0.09) | 0.49 (0.15) | 0.6 (0.2) | 1.0 (0.3) | 0.8 (0.5) | 0.6 (0.2) | 1.0 (0.1) | 1.17 (0.07) | 1.2 (0.3) | 1.2 (0.6) |
Inlet parameters (mg/L) | ||||||||||||||
SCOD | 1200 (40) | 970 (20) | 1010 (40) | 1100 (40) | 890 (20) | 1100 (30) | 890 (20) | 870 (30) | 910 (20) | 1210 (30) | ||||
NH4+-N | 32 (1) | 56 (1) | 54 (1) | 55 (1) | 51 (1) | 55 (2) | 50 (1) | 54 (2) | 57 (2) | 54 (2) | ||||
PO43−-P | 6 (1) | 7 (1) | 8 (1) | 9 (1) | 8 (1) | 8 (1) | 8 (1) | 7 (1) | 7 (1) | 9 (1) |
Ratio Test | COD:N: P Intake | Removal (%) | pH | Y X/S (mgVSS/mgCOD) | |||||
---|---|---|---|---|---|---|---|---|---|
COD | N | P | COD | N-NH4+ | P-PO43− | Initial | Final | ||
100:3 | 100 | 5.1 | 0.9 | 64.1 | 99.4 | 91.9 | 6.9 ± 0.1 | 6.9 ± 0.1 | 0.48 ± 0.01 |
100:4 | 100 | 7.4 | 1.2 | 58.6 | 98.8 | 100.0 | 6.9 ± 0.1 | 7.0 ± 0.1 | 0.68 ± 0.10 |
100:5 | 100 | 10.9 | 1.4 | 59.6 | 99.1 | 100.0 | 6.9 ± 0.1 | 6.9 ± 0.1 | 0.71 ± 0.03 |
100:6 | 100 | 6.4 | 1.0 | 74.2 | 98.8 | 100.0 | 7.0 ± 0.1 | 6.8 ± 0.0 | 0.46 ± 0.06 |
100:7 | 100 | 6.8 | 0.9 | 84.5 | 99.9 | 100.0 | 7.0 ± 0.1 | 6.9 ± 0.1 | 0.27 ± 0.09 |
DWW | 100 | 10.9 | 0.7 | 86.8 | 62.9 | 41.7 | 7.5 ± 0.1 | 7.8 ± 0.1 | 0.26 ± 0.05 |
LF | 100 | 1.4 | 0.3 | 30.6 | 52.4 | 28.7 | 4.5 ± 0.1 | 4.8 ± 0.1 | 0.21 ± 0.05 |
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Chacon-Aparicio, S.; Villamil, J.A.; Martinez, F.; Melero, J.A.; Molina, R.; Puyol, D. Achieving Discharge Limits in Single-Stage Domestic Wastewater Treatment by Combining Urban Waste Sources and Phototrophic Mixed Cultures. Microorganisms 2023, 11, 2324. https://doi.org/10.3390/microorganisms11092324
Chacon-Aparicio S, Villamil JA, Martinez F, Melero JA, Molina R, Puyol D. Achieving Discharge Limits in Single-Stage Domestic Wastewater Treatment by Combining Urban Waste Sources and Phototrophic Mixed Cultures. Microorganisms. 2023; 11(9):2324. https://doi.org/10.3390/microorganisms11092324
Chicago/Turabian StyleChacon-Aparicio, Sandra, John Alexander Villamil, Fernando Martinez, Juan Antonio Melero, Raul Molina, and Daniel Puyol. 2023. "Achieving Discharge Limits in Single-Stage Domestic Wastewater Treatment by Combining Urban Waste Sources and Phototrophic Mixed Cultures" Microorganisms 11, no. 9: 2324. https://doi.org/10.3390/microorganisms11092324