A Comparative Analysis of the Stomach, Gut, and Lung Microbiomes in Rattus norvegicus
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Site and Sample Collection
2.2. DNA Extraction and Bacterial 16S rRNA Gene Amplification
2.3. Library Construction and Sequencing
2.4. Bioinformatics Analysis
3. Results
3.1. R. norvegicus Stomach, Gut, and Lung Microbial Compositions
3.2. Comparing the Stomach, Gut, and Lung Microbiomes of R. norvegicus
3.3. R. norvegicus Stomach, Gut, and Lung Microbial Diversity
3.4. Variation of R. norvegicus Stomach, Gut, and Lung Microbial Communities
3.5. Evaluating Unique OTUs in Lungs
3.6. Evaluating Beneficial, Opportunistic, and Highly Pathogenic Bacteria at the Species Level
3.7. Identification of Potential Microbial OTU Biomarkers
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Group | No. of OTUs | No. of Seq |
---|---|---|
Gut | 301 | 99,055 |
Lung | 804 | 85,523 |
Stomach | 701 | 99,395 |
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Shah, T.; Wang, Y.; Wang, Y.; Li, Q.; Zhou, J.; Hou, Y.; Wang, B.; Xia, X. A Comparative Analysis of the Stomach, Gut, and Lung Microbiomes in Rattus norvegicus. Microorganisms 2023, 11, 2359. https://doi.org/10.3390/microorganisms11092359
Shah T, Wang Y, Wang Y, Li Q, Zhou J, Hou Y, Wang B, Xia X. A Comparative Analysis of the Stomach, Gut, and Lung Microbiomes in Rattus norvegicus. Microorganisms. 2023; 11(9):2359. https://doi.org/10.3390/microorganisms11092359
Chicago/Turabian StyleShah, Taif, Yuhan Wang, Yixuan Wang, Qian Li, Jiuxuan Zhou, Yutong Hou, Binghui Wang, and Xueshan Xia. 2023. "A Comparative Analysis of the Stomach, Gut, and Lung Microbiomes in Rattus norvegicus" Microorganisms 11, no. 9: 2359. https://doi.org/10.3390/microorganisms11092359