Competitive Analysis of Rumen and Hindgut Microbiota Composition and Fermentation Function in Diarrheic and Non-Diarrheic Postpartum Dairy Cows
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Management and Sample Collection
2.2. Sample Collection
2.3. Milk Yield, Composition, and Feed Digestibility Measurements
2.4. Ruminal and Fecal Fermentation Profiles and LPS Measurements
2.5. Rumen and Hindgut Microbiota Composition and Network Analysis
2.5.1. DNA Extraction and Amplicon Sequencing
2.5.2. Sequence Data Analysis
2.5.3. Microbiota Network Construction
2.6. Statistics
3. Results
3.1. Rumen and Hindgut Fermentation Profiles and LPS Concentrations
3.2. Ruminal and Fecal Bacterial Composition and Diversity
3.3. Relationship between Rumen and Fecal Bacteria and Fecal Water Content and Fermentation Profiles
3.4. Difference in Rumen and Fecal Bacterial Community Networks and Topologies between Two Groups of Cows
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Items 1 | Groups | SEM | p-Value | |
---|---|---|---|---|
LFS (n = 12) | HFS (n = 12) | |||
pH | 6.54 | 6.59 | 0.05 | 0.668 |
NH3-N mg/dL | 43.56 | 44.16 | 5.40 | 0.913 |
TVFA mM | 83.37 | 77.90 | 4.77 | 0.266 |
VFA (mol/100 mol) | ||||
Acetate | 58.82 | 61.83 | 1.43 | 0.048 |
Propionate | 26.13 | 22.07 | 1.38 | 0.008 |
Isobutyrate | 0.45 | 0.53 | 0.08 | 0.359 |
Butyrate | 12.16 | 13.06 | 0.61 | 0.155 |
Isovalerate | 1.04 | 1.09 | 0.14 | 0.732 |
Valerate | 1.41 | 1.43 | 0.14 | 0.868 |
A/P | 2.32 | 2.85 | 0.20 | 0.016 |
Fecal fermentation profile | ||||
NH3-N mg/g of feces | 4.04 | 2.61 | 0.31 | 0.020 |
TVFA µmol/g of feces | 63.14 | 81.06 | 5.23 | 0.002 |
VFA (mol/100 mol) | ||||
Acetate | 75.19 | 77.02 | 1.25 | 0.158 |
Propionate | 13.33 | 12.11 | 0.64 | 0.069 |
Isobutyrate | 1.33 | 0.82 | 0.15 | 0.003 |
Butyrate | 7.76 | 8.40 | 0.98 | 0.525 |
Isovalerate | 0.81 | 0.42 | 0.17 | 0.034 |
Valerate | 1.57 | 1.24 | 0.16 | 0.054 |
A/P | 5.71 | 6.49 | 0.37 | 0.046 |
Items 1 | Groups | SEM | p-Value | |
---|---|---|---|---|
LFS (n = 12) | HFS (n = 12) | |||
Fecal LPS (EU/g of wet feces) | 13,746.20 | 16,185.49 | 1049.20 | 0.260 |
Rumen fluid LPS (EU/mL) | 14,255.22 | 12,208.48 | 1645.38 | 0.550 |
Blood LPS (EU/mL) | 1.63 | 1.41 | 0.16 | 0.519 |
Blood LBP (mg/L) | 2.01 | 2.51 | 0.16 | 0.117 |
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Hao, Y.; Ouyang, T.; Wang, W.; Wang, Y.; Cao, Z.; Yang, H.; Guan, L.L.; Li, S. Competitive Analysis of Rumen and Hindgut Microbiota Composition and Fermentation Function in Diarrheic and Non-Diarrheic Postpartum Dairy Cows. Microorganisms 2024, 12, 23. https://doi.org/10.3390/microorganisms12010023
Hao Y, Ouyang T, Wang W, Wang Y, Cao Z, Yang H, Guan LL, Li S. Competitive Analysis of Rumen and Hindgut Microbiota Composition and Fermentation Function in Diarrheic and Non-Diarrheic Postpartum Dairy Cows. Microorganisms. 2024; 12(1):23. https://doi.org/10.3390/microorganisms12010023
Chicago/Turabian StyleHao, Yangyi, Tong Ouyang, Wei Wang, Yajing Wang, Zhijun Cao, Hongjian Yang, Le Luo Guan, and Shengli Li. 2024. "Competitive Analysis of Rumen and Hindgut Microbiota Composition and Fermentation Function in Diarrheic and Non-Diarrheic Postpartum Dairy Cows" Microorganisms 12, no. 1: 23. https://doi.org/10.3390/microorganisms12010023
APA StyleHao, Y., Ouyang, T., Wang, W., Wang, Y., Cao, Z., Yang, H., Guan, L. L., & Li, S. (2024). Competitive Analysis of Rumen and Hindgut Microbiota Composition and Fermentation Function in Diarrheic and Non-Diarrheic Postpartum Dairy Cows. Microorganisms, 12(1), 23. https://doi.org/10.3390/microorganisms12010023