Mosquito-Borne Arboviruses Occurrence and Distribution in the Last Three Decades in Central Africa: A Systematic Literature Review
by
, ,
Natacha Poungou
1,2,†,
Silas Lendzele Sevidzem
2,*,†,
Aubin Armel Koumba
3,
Christophe Roland Zinga Koumba
3,
Phillipe Mbehang
3,
Richard Onanga
4,
Julien Zahouli Bi Zahouli
5,
Gael Darren Maganga
4,
Luc Salako Djogbénou
6,
Steffen Borrmann
7,
Ayola Akim Adegnika
8,
Stefanie C. Becker
9,
Jacques François Mavoungou
3 and
Rodrigue Mintsa Nguéma
2,3 1
Ecole Doctorale Regionale en Infectiologie Tropical de Franceville (EDR), University of Science and Technique of Masuku (USTM), Franceville P.O. Box 943, Gabon
2
Laboratoire d’Ecologie des Maladies Transmissibles (LEMAT), Université Libreville Nord (ULN), Libreville P.O. Box 1177, Gabon
3
Département de Biologie et Ecologie Animale, Institut de Recherche en Ecologie Tropicale (IRET-CENAREST), Libreville P.O. Box 13354, Gabon
4
Center of Interdisciplinary Medical Analysis of Franceville (CIRMF), Franceville P.O. Box 769, Gabon
5
Centre d’Entomologie Médicale et Vétérinaire, Université Alassane Ouattara, Bouaké 01 BPV 18, Côte d’Ivoire
6
Université d’Abomey-Calavi, Institut Régional de Santé Publique, Ouidah P.O. Box 384, Benin
7
Institute for Tropical Medicine (ITM), University of Tübingen, 72074 Tübingen, Germany
8
Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné P.O. Box 242, Gabon
9
Institute for Parasitology, University of Veterinary Medicine Hannover, 30559 Hannover, Germany
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Microorganisms 2024, 12(1), 4; https://doi.org/10.3390/microorganisms12010004
Submission received: 19 September 2023
/
Revised: 13 October 2023
/
Accepted: 19 October 2023
/
Published: 19 December 2023
(This article belongs to the Special Issue Arboviruses 2.0)
Abstract
:Arboviruses represent a real public health problem globally and in the Central African subregion in particular, which represents a high-risk zone for the emergence and re-emergence of arbovirus outbreaks. Furthermore, an updated review on the current arbovirus burden and associated mosquito vectors is lacking for this region. To contribute to filling this knowledge gap, the current study was designed with the following objectives: (i) to systematically review data on the occurrence and distribution of arboviruses and mosquito fauna; and (ii) to identify potential spillover mosquito species in the Central African region in the last 30 years. A web search enabled the documentation of 2454 articles from different online databases. The preferred reporting items for systematic reviews and meta-analyses (PRISMA) and the quality of reporting of meta-analyses (QUORUM) steps for a systematic review enabled the selection of 164 articles that fulfilled our selection criteria. Of the six arboviruses (dengue virus (DENV), chikungunya virus (CHIKV), yellow fever virus (YFV), Zika virus (ZIKV), Rift Valley fever virus (RVFV), and West Nile virus (WNV)) of public health concern studied, the most frequently reported were chikungunya and dengue. The entomological records showed >248 species of mosquitoes regrouped under 15 genera, with Anopheles (n = 100 species), Culex (n = 56 species), and Aedes (n = 52 species) having high species diversity. Three genera were rarely represented, with only one species included, namely, Orthopodomyia, Lutzia, and Verrallina, but individuals of the genera Toxorhinchites and Finlayas were not identified at the species level. We found that two Aedes species (Ae. aegypti and Ae. albopictus) colonised the same microhabitat and were involved in major epidemics of the six medically important arboviruses, and other less-frequently identified mosquito genera consisted of competent species and were associated with outbreaks of medical and zoonotic arboviruses. The present study reveals a high species richness of competent mosquito vectors that could lead to the spillover of medically important arboviruses in the region. Although epidemiological studies were found, they were not regularly documented, and this also applies to vector competence and transmission studies. Future studies will consider unpublished information in dissertations and technical reports from different countries to allow their information to be more consistent. A regional project, entitled “Ecology of Arboviruses” (EcoVir), is underway in three countries (Gabon, Benin, and Cote d’Ivoire) to generate a more comprehensive epidemiological and entomological data on this topic.
1. Introduction
Mosquito-borne arboviruses are viruses that are transmitted by mosquitoes [1]. Arboviruses are maintained in nature by a cycle of biological transmission between a susceptible host and blood-feeding arthropods [2]. The clinical symptoms range from a mild febrile state to severe forms accompanied by haemorrhagic shock or encephalitis [3]. Arboviruses cause a mortality rate of 50% and an estimated 200,000 cases and 30,000 deaths each year, and yellow fever (YF) alone is of major public health concern in Africa and beyond [4]. In addition to YF, dengue (DENV) accounts for over 390 million infections per year [5], and other viruses, such as chikungunya (CHIKV), Zika virus (ZIKV), Rift Valley fever virus (RVFV), and West Nile virus (WNV), have also caused epidemics in Central Africa [6] and are also considered to be arboviruses of major public health concern in this region [7]. These medically important arboviruses have been reported to be silently and actively circulating during major epidemics in Central African countries such as Angola [8], Cameroon [6,9], the Central African Republic [10], Chad [11], Gabon [12], the Democratic republic of Congo [13], Guinea Equatorial [14], and the Republic of the Congo [15]. So far, despite the public health danger of these arboviruses in the subregion, regular surveillance is weak and/or almost absent [16], and some challenges for its non-implementation are as follows: (i) the lack of serological and molecular diagnostic platforms [8]; and (ii) the arbovirus topic is not a priority for research and surveillance for most countries due to other health, security, and political issues [16].
Landscape modifications and climate change as a result of an upsurge in anthropic activities over time in the tropics have modified the natural life cycle and occurrence of major arboviruses and their vectors [17]. Furthermore, in the central African region, the distribution of arboviral disease overlaps with that of competent arthropod vectors, and the increasing risk for its spread has been reported to be associated with deforestation, contact between humans and their wild counterparts, and with local and international transportation across porous terrestrial borders [18]. It appears that tropical species are now invading temperate zones, leading to the emergence of arboviruses that were previously only confined to well-defined regions of the globe. There are more than 3600 species of mosquitoes of the family Culicidae that occurs globally [19]. In the Central African region, two major invasive arboviruses vectors (Ae. aegypti and Ae. albopictus) have been frequently reported and in some countries of this region, and they are the main drivers of arbovirus spillovers [20], as reported in Cameroon [9].
Although arboviruses and their vectors represents a real public health danger in endemic settings, they are still a neglected topic, and limited studies have been conducted to present updated information on them for the Central African region. To fill this knowledge gap, the present study aimed to conduct a systematic literature review to update the occurrence and distribution of arboviruses of major public health concern, as well as associated spillover vectors in the Central African region, in the last 30 years to guide the control of these viruses and identify avenues for future studies.
2. Materials and Methods
2.1. The Study Region
The present study was conducted in eight countries (Angola, Cameroon, Chad, the Central African Republic, the Democratic Republic of Congo, Equatorial Guinea, Gabon, and the Republic of the Congo) of the Central African region. This region is located in the heart of the African continent. Central Africa is home for the second largest tropical rainforest in the world, with annual rainfall that ranges from 100–400 mm per year in the Sahel and over 1600 mm in the tropical rainforests. This ecosystem promotes the survival and proliferation of mosquitoes [1] and the propagation of the diseases they harbour.
2.2. Literature Search Strategy
The preferred reporting items for systematic reviews and meta-analyses (PRISMA, Berlin, Germany) [21] and the quality of reporting of meta-analyses (QUORUM) [22] criteria were applied in paper selection (Figure 1). The search for articles was conducted in the following databases: Google Scholar, Web of Science, PubMed, LILACS, Genesis Library and Z-Library. The following combinations of search keywords were used: “Gabon” OR “Cameroon” OR “Cameroun” OR “Belgian Congo” OR “Democratic Republic of the Congo” OR “Zaïre”, “Republic du Congo” OR “Republic of the Congo” OR “Angola” OR “Tchad” OR “Chad”, “Republic Centre Afrique” OR “Central African Republic” OR “Guinée Equatoriale” OR “Equatorial Guinea”, AND (“Mosquitoes” OR “mosquito-borne virus” OR “arbovirus” OR “arthropod-borne virus” OR “yellow fever” OR “chikungunya” OR “dengue” OR “Rift Valley Fever virus” OR “West Nile virus” OR “Zika” OR “alphavirus” OR “flavivirus” OR “bunyavirus”). Additional information was searched from the World Health Organization (WHO, Geneva, Switzerland) (http://www.who.int, accessed on 21 May 2023) and Centers for Disease Control and Prevention (CDC) (www.ecdc.europa.eu, accessed on 21 May 2023) databases.
2.3. Inclusion and Exclusion Criteria
All the papers collected were screened according to the relatedness to the topics of study and the inclusion/exclusion criteria were defined by consensus. The inclusion criteria were as follows: (i) studies conducted in Central Africa; (ii) references from review articles enabled the identification of more relevant papers. The exclusion criteria were as follows: (i) arboviruses detected in animals were not considered but were used in the discussion; (ii) incomplete information such as articles with only an abstract with no relevant information; (iii) studies conducted outside central Africa and reports published before 1993. The reason why we documented the relevant scientific literature from 1993 to 2023 (30 years) was because this period was characterised by major outbreaks in the different countries of Africa [23].
3. Results and Discussion
3.1. Papers Selected for the Review
The internet search resulted in the collection of 2454 papers, and only 164 conformed to the stipulated selection criteria (Figure 1). A complete database was created for the 164 relevant articles selected for this study.
3.2. Papers Retained by Country
The current study found that the majority of the papers available online on arboviruses and vectors are from Cameroon (58 (35.4%)), followed by Gabon (39 (23.8%)), and the smallest number recorded was from Equatorial Guinea (4 (2.4%)). The details on the distribution of the number of papers published within the study period by country can be found in Figure 2.
3.3. The Periodic Trend in Publication of Epidemiological and Entomological Data by Country
The number of relevant scientific papers on arboviruses and vectors published for the Central African region has improved for some countries and still remains scant for others. We made a graphical presentation of the situation by country, and we found, for Cameroon, that more papers appeared online between 2019 and 2022, with higher entomological studies than epidemiological and both (Figure 3). In Gabon, more publications appeared online between 2020 and 2021 and were dominated by entomological studies (Figure 3). In the Democratic Republic of Congo (DRC), more papers were published between 2018 and 2021 and were rather dorminated by epidemiological studies (Figure 3). In Angola, most papers appeared online in 2006 and were mostly epidemiological studies. For the other countries, such as the Central African Republic (CAR), Chad, Republic of the Congo (RoC), and Equatorial Guinea (EG), both epidemiological and entomological data were sparse and most of them were cross sectional. The low publication turnover for the different countries partly supports the contention that arboviruses are a neglected subject, and the available studies were conducted to confirm cases in suspected outbreak areas in the different countries. The weak trend in publication indicates the lack of regular surveillance of the diseases and vectors and the lack of conjoined and coordinated regional studies.
3.4. Occurrence of Arboviruses in the Central African Region
The six arboviruses of public health importance considered in this review belonged to three genera: (i) Alphavirus (CHIKV); (ii) Flavivirus (DENV, WNV, YFV, ZIKV); (iii) Phlebovirus (RVFV). Of these six arboviruses reported to be circulating in the Central African region, CHIKV and DENV have been detected in all the countries of this region [24], and the other four appear to variably occur in the different countries as follows.
3.4.1. Angola
The available and relevant information on arboviruses and associated vectors for Angola was documented in 17 papers. The details on each arbovirus targeted for this review are presented (Table 1) as follows.
DENV
Dengue circulation in Angola was first announced in the 1980s by infected travellers returning from Angola to the Netherlands [25]. In 2013, Angola reported its first locally acquired DENV cases [26]. During the 2013 epidemic, about 10% of cases and random cluster participants in Luanda, Angola’s capital city, displayed evidence of recent DENV infection [27]. Furthermore, the genetic study by Neto et al. [8] reported the circulation of DENV2 in Luanda. The detection of dengue during suspect outbreaks or in travelers in Angola was realised using three methods (rapid diagnostic tests, ELISA, and PCR/sequencing). A study conducted in the field used the rapid diagnostic kit (Dengue Duo, Standard Diagnostics) for the detection of the DENV non-structural protein 1 (NS1) (Morbidity and Mortality Weekly Report (MMWR) (http://www.cdc.gov/mmwr, accessed on 21 May 2023) (Ministry of Health Angola and the WHO). The non-structural protein (NSP) ELISA was also used to establish acute infections of DENV [26]. The Trioplex real-time RT-PCR was used to detect dengue, with the advantage that it is capable of differentiating ZIKV and discriminating CHIKV and DENV in coinfected individuals [28]. In addition, the nested PCR was conducted using primers targeting the C-prM region of DENV [29]. There is need for studies on vector competence of local potential mosquito populations for the transmission of DENV in hotspot areas.
YFV and CHIKV
In 1970 in Angola, outbreaks of YFV and CHIKV were reported [30]. The first YFV cases in Angola were reported in Luanda. On 13 April 2016, the WHO declared a YFV outbreak in Angola, and during the same period, the WHO also noted a case of RVFV in a man from China working in Luanda, the capital of Angola. Again, in May 2016, a 21 year old female traveller from Luanda to Tokyo tested positive for CHIKV. According to the distribution map proposed by Adam and Jassoy [24], Angola is endemic for YF and CHIKV. Although evidence of circulating YFV and CHIKV was established using high throughput diagnostic techniques in Angola, the issue of the lack of regular surveillance remains. The occurrence of CHIKV was confirmed using the Trioplex real-time RT-PCR [28,31]. The YFV cases were detected using RT-PCR, with primers targeting the 5′ non coding region, and positive samples were further tested using the pan-flavivirus RT-PCR, targeting the flavivirus NS5 gene region using specific primers (FU18993F and cFD29258R [32]. Additionally, although Aedes, Anopheles spp., and Culex pipiens vector compatibility was studied in Angola [33,34], an interesting study was conducted to show the implication of Aedes mosquitoes in the transmission of YFV during the 2016 outbreak [35].
3.4.2. Cameroon
All six medically important arboviruses targeted in this review have been reported in Cameroon (Table 1). This study found 58 papers published online on these arboviruses and associated vectors. The situation of the different arboviruses of Cameroon is as follows.
CHIKV
In Cameroon, CHIKV was reported in most regions, with high prevalence reported in the northwest (51.4%) [36], and others recorded varing prevalence as follows: Littoral region (12.6–59.4%) [37]; Central region (3–59.4%) [36]; and South West region (4–63%) [38]. However, Cameroon has already been reported as a CHIKV-endemic area with high transmission risk [24]. The 2006, a CHIKV outbreak in Cameroon was confirmed via real-time RT-PCR and partial sequencing of the envelope gene [39]. During the 2006 CHIKV outbreak in the West region of Cameroon, the persistence of anti-CHIKV IgM antibodies was reported in the local population, and entomological studies revealed high relative abundance of Aedes africanus [9]. The risk of CHIKV emergence and re-emergence in the west region of Cameroon was supported by an entomological study that showed high species richness and abundance of competent vectors in this part of the country [40]. Interestingly, a study conducted at the border between Cameroon and Gabon showed that patients coming to seek health services in Kyé-ossi in Cameroon from the neighboring town of Gabon (Bitam) were diagnosed positive with CHIKV, indicating the possibility of cross-border transmission between the two countries [18]. The transmission risk of arboviruses at borders between central African countries needs to be studied.
DENV
Dengue was found in all the epidemiological studies for Cameroon and was detected in all the 10 regions of the country. The prevalence, by region, or reports of this virus is as follows: Littoral (3.8–68.3%) [41,42,43], Far North (6.7–14.36%) [44,45], West (6.14–14.36%) [45,46], Center (3–45.45%) [45,47], South West (2.5–74%) [6,38] and South (0.5–14.28%). Similar to CHIKV, Dengue is also endemic in the whole of Cameroon with high transmission risk [24]. The nationwide occurrence of DENV has been shown using several diagnostic methods (ELISA and PCR/sequencing). At the regional level, DENV was detected among inpatients using ELISA [48]. In the rural town of Kribi in the south region of Cameroon, MAC-ELISA and the CDC Trioplex real-time RT-PCR were used to show the circulation of DENV [49]. Moreover, the amplification of the partial E gene (expected band size of 250 bp) for DENV revealed the occurrence of this virus in the economic capital city (Douala) of Cameroon [41]. The trasmission risk of DENV in this town has already been proven by Kamgang et al. [50] where the two competent vectors (Ae. aegypti and Ae. albopictus) were identified and their vector roles established for three major towns of Cameroon (Garoua, Douala and Yaounde). Furthermore, a study in the political capital city (Yaounde) of Cameroon revealed high ecological adaptation of Ae. aegypti and Ae. albopictus and the potential risk for the transmission of arboviruses [51]. The vector ecology and competence of these two vectors of DENV has already been studied in Cameroon [40,50,52,53].
YFV
Yellow Fever Virus was detected in two hotspot regions with following prevalences notably: North (25.5%) region [54] and South West region (4–72%) [6,38]. YFV is endemic in Cameroon, with high risk of transmission [24]. It is important to add that the YFV outbreak in Garoua town of the North region of Cameroon was first identified via serology using the MAC-ELISA IgM test, and positive samples were airlifted to the WHO reference laboratory in Dakar, Senegal, for confirmation [54]. The circulation of YFV in this local population is not surprising; Kamgang et al. [50] already identified and established the role of competent Aedes vectors in this town. Furthermore, the analysis of laboratory tests results from 2010 to 2020 in Cameroon revealed sustained YFV transmission [55]. It should be noted that Cameroon has a national YFV surveillance system that is planned and implemented by the expanded programme on immunization (EPI) of the Ministry of Public Health, with support from foreign donors.
ZIKV
Most of the studies found online concerning the arboviruses of Cameroon frequently reported ZIKV. The prevalence differed in each region as follows: Southwest (11.4%) [6]; Littoral (10–26.2%) [26,44]; East (7.6%) [56]; Far North (2–4.8%) [56]; and Adamawa (2%) [56]. Zika virus has already been reported to be endemic in Cameroon and with high transmission risk [24]. The studies showing the burden of ZIKV in populations of the different regions of Cameroon were conducted using the CDC Trioplex real-time RT-PCR assay, which is capable of discriminating ZIKV from DENV/CHIKV [18]. In fact, it has already been reported via a transmission study that Ae. aegypti and Ae. albopictus are susceptible to infection and spread of ZIKV in Cameroon [57].
WNV
The reports on WNV for Cameroon are scant, and it was only reported in the South West region of Cameoron, with a prevalence range of 3–82% [38]. It is clear from the study of Mayi et al. [40] on vector adaptability conducted in an area bordering the South West region of Cameroon that there was high species richness and diversity of WNV-competent vectors that could represent a transmission risk in the area. However, in the town of Garoua in the North region, a study identified competent vectors of RVFV and WNV [58].
3.4.3. Central African Republic
The CAR has been victim of attacks of 19 arboviruses in the past, and recently, 3 arboviruses were involved in fatal cases: in 1983, WNV was isolated in four patients; two serious cases of YFV occurred in 1985 and 1986; and from 1983 to 1986, RVFV was identified in patients who died from hemorrhagic fever [59]. The details on the different arboviruses are presented (Table 1) in the following paragrahs.
CHIKV
Genetic analysis by Tricou et al. [60] confirmed the circulation of CHIKV in the 1970s and 1980s. A serological survey of antibodies to arboviruses was carried out in the human population of the southeast part of CAR in April 1979, and CHIKV was detected to be actively circulating in adult population [61]. The distribution map of CHIKV by Adam and Jassoy [24] shows that CAR is an endemic country for this virus. It is necessary to underline here that CHIKV was frequently studied, and this could be due to the fact that major outbreaks were associated to it. The evidence of circulating CHIKV in CAR was conducted using PCR, where two sets of primers (E1-10145F/E1-11158R and E2-8458F/E2-9240R) were used to amplify the partial sequences of the structural polyprotein gene in the E1 and E2 coding region [62]. Furthermore, an entomological study supported the transmission risk of endemic CHIKV via anthropophilic Ae. aegypti and Ae. albopictus [63,64].
RVFV
An RVFV study conducted in cattle and humans in Bangui reported an overall seroprevalence of anti-RVFV IgM antibodies of 1.9% and that of IgG antibodies of 8.6%. IgM antibodies were found only during the rainy season, but the frequency of IgG antibodies did not differ significantly by season. No evidence of recent RVFV infection was found in 335 people considered at risk; however, 16.7% had evidence of past infection [65]. In another study conducted on cattle, it was found that antibodies to RVFV virus were found in about 8% of adult cattle [66]. The presence of antibodies of CHIKV in cattle indicates their possible role as a reservoir of the disease in Bangui.
YFV, DENV, and ZIKV
In the Central African Republic, since 2006, YFV cases have been notified in the provinces of Ombella-Mpoko, Ouham-Pende, Basse-Kotto, Haute-Kotto, and in Bangui, the capital, which is also an Aedes spp.-endemic area. However, the presence of the YFV vectors in the capital city of CAR represents a risk for the spread of the disease. To the best of our knowledge, little or no updated information on YFV, DENV, and ZIKV has been published on the burden of these arboviruses in CAR. However, a distribution map on these three arboviruses of public health concern by Adam and Jassoy [24] showed that CAR is an endemic country for these arboviruses and its vectors. We noticed from the published information on arboviruses of CAR that more entomological studies were conducted to show potential spillover, but epidemiological evidence was scant, probably due to lack of diagnostic capacity and the health care priority being focused on other diseases. An interesting study from 1973 to 1983 in CAR revealed the vector competence status of several species of Aedes, Culex, and Anopheles in the spread of the six medically important arboviruses considered in this current review [67]. Moreover, updated information on competent vectors of arboviruses from 2006 to 2010 was reported [10]. Furthermore, the competent vectors of YFV were identified in CAR [68].
3.4.4. Chad
Three important arboviruses (DENV, YFV, and RVFV) have been reported to occur in Ndjamena (Table 1), the capital city of Chad. Only six papers were found eligible for Chad and were included in the study. The weak publication turnover of Chad could be due to the lack of diagnostic capacity as in most central African countries, where samples are usually sent for further confirmation outside the country, and due to the fact that health care priority is oriented towards other epidemics.
DENV
Information on dengue in Chad is not documented and no available evidence on its occurrence was found for the period from 1993 to 2023, but the distribution map of Adam and Jassoy [24] indicates the presence of this arbovirus in this country. The occurrence of competent mosquito vectors have already been reported in the DENV outbreak areas of Chad, and this portrays the risk for the transmission of arboviruses [69].
YFV
A low prevalence (0.28%) of YF was obtained from jaundice patients in Ndjamena from 2015 to 2020 during a non-outbreak period [70]. Chad has also been reported to be a yellow-fever-endemic area with high transmission risk [24]. It is important to add that the detection of YFV was carried out by MAC-ELISA-CDC [70].
RVFV
This is a zoonotic febrile disease that affects livestock and humans and was first reported in Chad in 1967 [71] and in the same period in Cameroon; since then, this disease has spread beyond the subregion. Apart from this preliminary report, another report by Durand et al. [72] revealed a prevalence rate of 4% in French troops. The evidence of circulating RVFV among these French soldiers was carried out using ELISA and confirmed via real time PCR/sequencing using primers targeting the L, M, and S regions of the genome [72]. In fact, the competent vectors of this arbovirus have already been reported [69].
3.4.5. Democratic Republic of Congo
The available and relevant information on arboviruses and associated vectors for the Democratic Republic of Congo was documented in 19 papers. The details on each arbovirus targeted for this review is presented (Table 1) as follows.
YFV
Following the indepence of the DRC in 1960, YF epidemics have been reported in all 26 provinces of the country. A two-year survey (2013 to 2014) reported a YFV prevalence of 31.5% among children in the DRC [73]. From 5 December 2015 to November 2016, a large YF outbreak affected Angola and the DRC, with 7334 suspected cases, of which 962 have been confirmed, and 393 deaths were reported to the WHO as of 28 October 2016 [74,75]. According to the updated distribution map of Adam and Jassoy [24], the DRC is a YF-endemic area with high transmission risk [75,76]. A recent report on YF in the DRC was conducted in Kinshasa, its capital city, with a seroprevalence range of 6–73% [73,77] (Table 1). The occurrence of YFV in the capital city of this country was not astonishing, as it is known that densely populated cities, where high densities of mosquitoes coexists with city inhabitants, are a favouratble milieu for an epidemic of massive proportions. Moreover, the existence of a high density of competent Aedes vectors of YFV, already identified in the DRC, is the major driver of major epidemics [78].
CHIKV
In the last two decades, Kinshasa, the capital of the DRC, experienced CHIKV epidemics in the years 1999 and 2000, with an estimated 50,000 reported cases [79]. In addition, also in Kinshasa, another outbreak occurred in 2012 [80]. Apart from Kinshasa, other provinces where CHIKV has been reported are Kisangani [81] and Matadi [78]. According to the Aedes spp. and the CHIKV distribution maps published in Adam and Jassoy [24], the DRC is a CHIKV-endemic area with high transmission risk [76]. The evidence for the occurrence and spread of CHIKV in the DRC was obtained through high-throughput diagnostic approaches such as PCR/sequencing, where a CHIKV-specific RT-qPCR was performed using primers targeting a 77 bp portion of the non-structural protein 1 (NSP-1), as described by Planning and collaborators [78]. An entomological investigation led to the identification of Ae. albopictus as the primary vector of CHIKV [78]. Similarly, another study used primers that rather targeted the E1/3′UTR region, as evidence of the re-emergence of CHIKV in DRC [82]. The principal vector involved in CHIKV transmission in the DRC has been reported to be Ae. albopictus [83,84].
DENV
Dengue fever virus is one of the common mosquito-borne viruses in the DRC, and cases of this disease has been reported in some hotspot provinces such as Kisangani [81] and Kinshasa [73,80,85,86] via seroepidemiological studies [73,87]. Furthermore, serotyping information on the circulating DENV in the DRC was not available until a survey reported 16 DENV-1 and DENV-2 cases from 2003 to 2012 [88]. Genetic analysis revealed that the DENV-1 strain that caused the 2013 epidemic in Angola also circulated in the DRC in 2015 [89]. Three serotypes of DENV (DENV-1, DENV-2, and DENV-3) have been recorded in the DRC, the most frequent being serotype DENV-1 [73]. In Kisangani, DENV co-circulated with CHIKV during the WNV outbreak of 1998 [81]. In Kinshasa, co-occurrence of dengue and chikungunya was reported during the 2012 outbreak [80]. To conclude, the DENV updated map for sub-Saharan Africa (SSA), published in Adam and Jassoy [24], shows that the DRC is a DENV-endemic area with high transmission risk [76]. The evidence of DENV in the population was confirmed via PCR/sequencing, where the DENV-1 was detected using pan-flavivirus nested RT-PCR with primers targeting the non-structural protein 5 (NS5) gene [89]. Similarly, another study for the confirmation of DENV-1 rather used specific primers targeting the E gene [85]. The circulation of DENV-1 was not surprising, as entomological studies reported the presence of competent vectors in the DRC [83,84].
ZIKV
Only a few studies present relevant information on the burden and distributon of Zika in the DRC, and only one study reported on its occurrence. A serological study from 2013 to 2014 showed a prevalence rate of 3.5% for ZIKV antibodies in sud-Ubangi [73]. Another study by [88], for the period 2003 to 2011, showed a negative test result for ZIKV using the polymerase chain reaction (PCR) method. The occurrence of Zika virus in the DRC has also been shown in the updated distribution map for ZIKV for SSA [24].
RVFV
In 1998, in the Kisangani area of the DRC, RVF has was reported in humans with a low prevalence of 4% [81]. As a zoonotic arbovirus, it has also been reported in domestic animals such as cattle, where in 2009, a seroprevalence of 20% was reported in this animal species in Katanga [90]. Moreover, a seroprevalence rate range of 2–16% among cattle was reported in the Nord-Kivu, Sud-Kivu, and Ituri provinces from the Eastern region [91]. A transmission risk study conducted in 2014 revealed that Aedes mosquitoes harbored RVFV [92].
WNV
From the available information on WNV of the DRC, only one paper presents relevant information on clinical cases of the disease, and this was in 1998, when a high seroprevalence of 66% was reported in Kisangani [81]. Other information on this virus has been obtained from research on wild animals to establish their potential epizootiological role in its spread to humans, where WNV antibodies were detected in Haut-Uelé Province in chimpanzee [93], bufalo, and elephant in the Garamba National Park [91].
3.4.6. Equatorial Guinea
The available and relevant information on arboviruses and associated vectors for Equatorial Guinea (EG) was documented in only four papers. The weak publication frequency in EG could be due to the lack of diagnostic facilities, as in most central African countries, where samples are mostly sent for further confirmation outside the country, and also due to the fact that the health care priority is oriented towards other epidemics. It is important to add that the use of less sensitive and specific techniques, such as rapid diagnostic tests and ELISAs, could lead to the under-reporting of the occurrence and burden of some key arboviruses in many African countries. The details on each arbovirus targeted for this review are presented (Table 1) as follows.
CHIKV, DENV, and YFV
From the best of our knowledge, the only arboviruses that have been reported in EG are CHIKV, DENV, and YFV. Chikungunya was first detected in 2002 and 2003 [94]. In 2006, one of the three travelers returning from EG was diagnosed as positive for CHIKV in Spain [14]. Inaddition, EG is known to be CHIKV-endemic [24]. Similarly, only one study presents the distribution map of DENV and YFV of EG, and it shows that the country is endemic for the two arboviral types [24]. The PCR/sequencing techniques provided evidence for the circulation of CHIKV, where sequences of amplified fragments corresponding to 195 bp of the non-structural protein 4 gene of alphaviruses identified a homogenous cluster of this arbovirus in the 2002 and 2006 outbreaks [14]. In order to obtain a sequence with more phylogenetic information, primers designed by Powers et al. were used to amplify a fragment of a region of the envelope 1 (E1) gene often used for CHIKV phylogenetic analysis [14]. During major arboviral outbreak periods, entomological reports showed the wide spread of Ae. albopictus vectors in Bioko in EG [95].
3.4.7. Gabon
In Gabon, arboviruses and associated vectors have been reported in 39 papers. The publication trend has evolved positively for epidemiological studies, with a high number registered in 2022. All six arboviruses of public health concern have been identified (Table 1), but their prevalence and distribution differed with each province as follows.
CHIKV
This virus was reported in the whole of Gabon [96], and two important outbreaks occurred in 2007 and 2010 [97] in the Estuaire province of the capital city (Libreville) of Gabon. In Libreville, the prevalence range was 3–86% [98], followed by Haut-Ogooue (45.2–62.3%) [97], and then Ogooue-Lolo (28.7%) [99]. Recent reports on CHIKV are from the Moyen-Ogooue province, with a prevalence range of 0.6–61.2% [6,100]. Chikungunya is endemic in Gabon, with high transmission risk [24]. The evidence of circulating CHIKV in Gabon was confirmed using high-throughput PCR/sequencing using specific primers (OP16 and OP17) [101]. Another study targeted the E1 gene for CHIKV, as well as the 3′UTR region, during the 2007 and 2010 CHIKV/DENV outbreaks in Libreville, the capital city of Gabon [99]. It is important to underline that the 2010 arbovirus outbreak in Gabon was driven by CHIKV and DENV [96,100]. This period (2007 and 2010) was characterised by the invasion and wide spread of competent Aedes vectors in Libreville [102,103,104].
DENV
Similar to CHIKV, DENV in Gabon was identified in all the regions surveyed [96,105] and has been reported to be endemic [24]. The DENV hotspot areas are Moyen-Ogooue (12.3–88.24%) [7,106,107], Haut-Ogooue (12.2%) [97], and Estuaire (4–21.4%) [98]. The evidence of DENV-2 circulation in Gabon by PCR/sequencing was carried out using primers to amplify the envelope (E) gene (758 bp; genome position 1503–2260 nt). Similarly, consensus DENV-1 and DENV-3 PCR fragments of the E gene corresponding to a 472 bp fragment of DENV-1 (genome position 1234–1705 nt) and to a 935 bp fragment of DENV-3 (genome position 1256 to 2190 nt) were amplified [105]. Another study was conducted to show the evidence of DENV-3 having amplified the full length of the envelope gene (1479 bp) [106]. Entomological studies found that Ae. aegypti and Ae. albopictus were associated with CHIKV and DENV-2 [98,108]. In 2021, the re-emergence of DENV, CHIKV, and ZIKV was established via PCR/sequencing using primers targeting the envelope of dengue virus serotype 1 (1485 bp). The updated entomological studies in both urban [109,110] and sylvan environments [111] showed high density and species richness of Aedes mosquito vectors in Gabon. Another interesting finding in Gabon was that which identified genes underlying specific resistance of DENV-1 and DENV-3 in Ae. aegypti [104].
RVFV, YFV, WNV, and ZIKV
The reports on RVFV, YFV, WNV, and ZIKV for Gabon are scant; only one paper reported the presence of these four arboviruses, and only in the Moyen-Ogooue province, with varying prevalences: YFV (60.7%); ZIKV (40.3%); WNV (25.3%); and RVFV (14.3%) [7]. The evidence from serology/RT-PCR tests shows the recent circulation of the six medically important arboviruses considered in this study [7]. The evidence of circulating ZIKV was made through PCR/sequencing, where the non-structural protein 3 (1851 bp) was targeted, and then further screening targeted non-structural proteins (772 bp) and envelope (750) genes of ZIKV [12]. Moreover, the evidence of circulating ZIKV in Gabon was made by amplifying the E and NS3 region using specific primer sequences already published for E genes (ZIK-ES1/ZIK-ER1, ZIK-ES2/ZIK-ER2) and NS3 genes (ZIK-NS3FS/ZIK-NS3FR and ZIK-X1/ZIK-X2), and the entomological part of this study revealed Ae. albopictus as the primary vector [20]. It is important to state that the RVFV reported in Gabon was only confirmed via serology and the presence of competent vectors [112].
3.4.8. The Republic of the Congo
The available and relevant information on arboviruses and associated vectors for the RoC was documented in 10 papers. Evidence of the circulating arboviruses in the RoC was based on rapid diagnostic tests, ELISA, and RT-PCR/sequencing. It is known that the sensitivity and specificity of any rapid antibody (IgG or IgM) ELISA or RT-PCR could be of limited value during the initial phase of the transmission window of the disease, as the level of viraemia and IgM antibody titres may be below the limits of detection [113]. The details for each arbovirus targeted for this review is presented (Table 1) as follows.
CHIKV
In January 2019, an outbreak of CHIKV fever was reported near Pointe-Noire. This study found a novel CHIKV strain and established the presence of the A226V substitution and close relation with Aedes aegypti-associated Central Africa chikungunya strains [114]. Similarly, in 9 February 2019, during the CHIKV outbreak, investigations found two new CHIKV sequences of the East/Central/South African (ECSA) lineage, clustering with the recent enzootic sub-clade 2, showing the A226V mutation. Entomological surveys reported one Ae. albopictus pool to be RT-PCR positive [15]. The establishment of the occurrence of CHIKV in the RoC was conducted using two methods (rapid diagnostic test (RDT) and RT-PCR/sequencing). The RDT for specific IgG and IgM detection (STANDARD F Chikungunya IgM/IgG FIA SD BIOSENSOR, Chungcheongbuk, Republic of Korea) was used, and RT-PCR/sequencing was conducted using primers designed by referring to the sequences of the Pakistan-07/2016 CHIKV isolate complete genome [15]. Moreover, the evidence of the CHIKV 2011 outbreak in the RoC was obtained via RT-PCR, where primers previously designed to sequence the LR2006 OPYI CHIKV strain were used to generate PCR products [115]. Aedes albopictus was identified to be the primary vector of CHIKV in the RoC [15]. The re-emergence of CHIKV in the RoC was due to the wide spread and dense population of Ae. albopictus, as already reported [116].
DENV
Although DENV is one of the frequently reported arboviruses in the Central African subregion, to the best of our knowledge, no relevant information has been presented on its burden and occurrence in the RoC. However, the distribution map of DENV for SSA by Adam and Jassoy [24] clearly shows that the RoC is endemic for this virus and its Aedes spp. Vectors [116].
ZIKV
The lone study reporting the occurrence of ZIKV in the RoC was conducted on 386 serum specimens from volunteer blood donors in 2011 from rural and urban areas of the Republic of the Congo. The result of this study showed a low ZIKV seropositivity rate (1.8%) [117]. The occurrence of competent vectors (Ae. aegypti and Ae. albopictus) of ZIKV is evidence of risk for its transmission in the RoC [118].
3.5. Distribution of Arboviruses in the Central African Subregion
Chikungunya and Dengue were the most frequently detected of the six studied arboviruses of medical importance. Indeed, some countries have already witnessed historic epidemic waves of CHIKV; for instance, in Cameroon in 2006, in Gabon (2007 to 2010), in Congo Brazzaville in 2011, and in the DRC in 2019. The circulating arboviruses in the different Central African countries are presented in Table 1. The widespread distribution of CHIKV and DENV could be attributed to the suitable ecological variables for its vectors, and, of course, it has already been reported that DENV is the most prevalent of all arboviruses [119,120]. Although RVFV cases have already been reported in clinical cases in countries such as Chad, CAR, Gabon, and the DRC, data are still scant, but in a country such as Cameroon, information is mostly available for animal species (cattle, sheep, and goats) [121,122]. The free circulation of livestock and people in the Central African regional corridor could be the main driver of the circulating strains of major arboviruses in countries of this region [6]. The circulation of RVFV in domesticated ruminants in countries of this region could indicate a possible risk of human exposure to zoonotic strains [123]. Moreover, information on WNV was also poorly documented, with clinical cases reported in Cameroon, Gabon, and the Democratic Republic of Congo. Moreover, information on WNV in animals is scant and needs to be documented. The detection discrepancies between countries could be multifactorial, as follows: (i) lack of knowledge; (ii) low or lack of diagnostic capacity; and (iii) poor surveillance systems. The lack of data from the Central African subregion makes it difficult to generate robust and quality field epidemiological and entomological information that could inform us of the patterns and drivers of arthropod-borne diseases transmission [124,125].
Table 1.
Occurrence and burden of major arboviruses in different countries of the Central African subregion from January 1993 to June 2023.
Table 1.
Occurrence and burden of major arboviruses in different countries of the Central African subregion from January 1993 to June 2023.
| Country | Site (Region, Province, City) | Arbovirus | Diagnosis | Proportions (%) | References |
|---|---|---|---|---|---|
| Gabon | Estuaire | CHIKV | P, S, S | 3–86 | [97,98,101] |
| DENV | P, S, S | 4–21.4 | [97,98,101] | ||
| Moyen Ogooue | CHIKV | S + P, S, S | 0.6–61.2 | [7,100,126] | |
| DENV | S + P, S, S + P, S + P | 12.3–88.24 | [7,100,106,107] | ||
| RVFV | S + P | 14.3 | [7] | ||
| YFV | S + P | 60.7 | [7] | ||
| WNV | S + P | 25.3 | [7] | ||
| ZIKV | S + P | 40.3 | [7] | ||
| Haut Ogooue | CHIKV | p | 45.2–62.3 | [99] | |
| DENV | P | 12.2 | [99] | ||
| Ogooue Lolo | CHIKV | P | 28.7 | [99] | |
| Woleu Ntem | CHIKV | P | 0.5 | [18] | |
| nationwide | CHIKV | P, P | 35.6–86 | [96,127] | |
| DENV | P, P | 0.2–94.8 | [96,105] | ||
| 212–220 villages | DENV | S | 0.5 | [128] | |
| RVFV | S | 3.3 | [112] | ||
| Cameroon | East | ZIKV | S | 7.6 | [56] |
| Littoral | CHIKV | S | 12.6–59.4 | [36,37] | |
| DENV | S + P + R, S + P + R, P, P, S + R, R + S, R + P | 3.9–68.3 | [37,41,42,43,44,45,47] | ||
| ZIKV | S + P + R, S | 10–26.2 | [37,56] | ||
| South | DENV | P, S + P | 0.5–14.28 | [18,129] | |
| North | YFV | S | 25.5 | [54] | |
| Far North | DENV | S + P + R, S + P + R | 6.7–14.36 | [44,45] | |
| ZIKV | S | 2–4.8 | [56] | ||
| Adamawa | DENV | S + R, R | 4.7–6.89 | [47,48] | |
| ZIKV | S | 2 | [56] | ||
| West | CHIKV | S + P | 15.7 | [46] | |
| DENV | S + R, S +R, S + P, P + R | 6.14–14.36 | [44,45,46,47] | ||
| Center | CHIKV | S, S + P + R, S + P | 3–59.4 | [36,37,39] | |
| DENV | S + P + R, S + R, S + R, P + R, S + P | 3–45.45 | [37,39,44,45,47] | ||
| ZIKV | S | 3.3 | [56] | ||
| North West | CHIKV | S | 51.4 | [9] | |
| South West | CHIKV | S, S | 4–63 | [6,38] | |
| ZIKV | S | 11.4 | [6] | ||
| DENV | S, S | 2.5–74 | [6,38] | ||
| YFV | S | 4–72 | [38] | ||
| WNV | S | 3–82 | [38] | ||
| Democratic Republic of Congo | Matadi | CHIKV | S + P + R | 83.2 | [78] |
| Kisangani | WNV | S | 66 | [81] | |
| CHIKV | S | 34 | [81] | ||
| DENV | S | 3 | [81] | ||
| RVFV | S | 4 | [45] | ||
| kinshasa | DENV | S, R, P, S + P + R, S + P | 0.4–8.1 | [73,80,85,86,88] | |
| YFV | S, S + P | 6.0–73 | [73,77] | ||
| CHIKV | S, R, S + P, S, P, P | 0.1–49.7 | [73,80,82,88,130,131] | ||
| Sud-Ubangi | ZIKV | S | 3.5% | [73] | |
| Republic of the Congo | Brazzaville | CHIKV | P, S + P | 11.7–71 | [115,132] |
| Pointe-Noire | P, S + P | - | [15,114] | ||
| - | ZIKV | S | 1.8 | [117] | |
| - | DENV | S + P | - | [24] | |
| Angola | Luanda | DENV | P, P + R, S + P | 11.1–94.4 | [8,31,133] |
| CHIKV | P, P | 7 | [8,134] | ||
| 13 provinces | YFV | S | 70 | [135] | |
| Equatorial Guinea | Bata | CHIKV | P | 1.1–33.3 | [14] |
| Chad | N’Djamena | YFV | S + P, S + R | 0.28 | [24,70] |
| RVFV | P | 4 | [72] | ||
| DENV | S + P | - | [24] | ||
| Central African Republic | Bangui | YFV | S + P | 6.5 | [136] |
| RVFV | P | 1.9–16.7 | [65] | ||
| CHIKV | P | - | [60] |
Chikungunya virus (CHIKV); dengue Virus (DENV); Zika Virus (ZIKV); yellow fever Virus (YFV); Rift Valley fever virus (RVFV); West Nile virus (WNV). (-) not available; RNA detection (P); rapid diagnostic test (R); serology (S); serological and RNA detection in the same paper (S + P); serology, RNA detection, and rapid diagnostic test (S + P + R). The order of tests separated by commas in the diagnosis column corresponds to the references in the square brackets of the references column.
3.6. The Mosquito Fauna of the Central African Region from 1993 to 2023
After a thorough online search, we documented >248 species of mosquitoes, regrouped under 15 genera in decreasing order of magnitude as follows: Anopheles (n = 100), Culex (n = 56), Aedes (n = 52), Uranotaenia (n = 12), Coquillettidia (n = 10), Eretmapodites (n = 5), Ficalbia (n = 4), Mansonia (n = 2), Mimomyia (n = 2), Ochlerotatus (n = 2), Lutzia (n = 1), Orthopodomyia (n = 1), and Verrallina (n = 1), but Finlayas and Toxorhinchites species were unidentified (Figure 4, Table 2).
From the current study, it is clear that the genus Culex constituted the most diverse group with the highest species frequency, and this could be accounted for by their high adaptability in different agroecological settings and their ability to colonise diverse microhabitat-types for breeding, survival, and proliferation. The genus Aedes was the second-most-frequent group, with invasive species such as Ae. aegypti and Ae. albopictus that codominated rural and urban spaces [111]. These two species were frequently identified in all countries presenting data on potential mosquito fauna of arboviruses. Although Ae. aegypti has been frequently reported [50,137], Ae. albopictus usually occurs in higher proportions in the Central African Region [111,131] (Table 3). Despite the fact that studies reporting circulating arboviruses in mosquitoes in the Central African region are scant, papers published elsewhere in West Africa, particularly in the Ivory Coast, reported very low infection rates in local Ae. aegypti [138]. The occurrence of different species in countries of this region could be an indication of an eventual spillover of arboviruses [139].
Table 2.
Mosquito fauna reported in some Central African countries from January 1993 to June 2023.
| Species | Countries | References | |||||||
|---|---|---|---|---|---|---|---|---|---|
| CMR | RoC | Gab | CAR | DRC | EG | Ang | Cha | ||
| Aedes aegypti | + | + | + | + | + | + | + | + | [10,20,29,34,40,46,50,51,52,53,57,58,68,69,78,95,96,99,102,103,104,108,109,111,114,115,118,131,137,139,140,141,142,143,144,145,146,147,148,149,150,151,152,153,154,155,156,157,158,159,160,161,162,163,164,165,166,167,168] |
| Aedes abnormalis | + | [67] | |||||||
| Aedes africanus | + | + | + | [9,10,40,67,68,95,99,139,141,148,150,168,169,170,171,172] | |||||
| Aedes albopictus | + | + | + | + | + | + | + | + | [9,10,15,20,29,52,53,57,58,69,78,83,95,96,102,111,112,113,114,115,116,117,118,119,120,121,122,123,124,125,126,127,128,129,130,131,132,133,134,135,136,137,139,141,142,143,144,145,147,148,149,152,161,162,163,166,167,170,172,173,174,175] |
| Aedes alternans | + | [69] | |||||||
| Aedes argenteopunctatus | + | + | [10,40,139,141,171] | ||||||
| Aedes australis | + | [69] | |||||||
| Aedes caspiua | + | [69] | |||||||
| Aedes centropunctatus | + | [141] | |||||||
| Aedes cinerus | + | [69] | |||||||
| Aedes circum | + | + | [10,40,58,139] | ||||||
| Aedes circumluteocus | + | + | + | [40,139,171,172,176] | |||||
| Aedes contigus | + | [53,170] | |||||||
| Aedes cumminsii | + | + | [10,141] | ||||||
| Aedes dalzieli | + | + | + | [58,141,172] | |||||
| Aedes dendrophilus | [10] | ||||||||
| Aedes domesticus | + | [10,139] | |||||||
| Aedes dufouri | + | [69] | |||||||
| Aedes fraseri | + | [40,139,171] | |||||||
| Aedes flavifrons | + | [122] | |||||||
| Aedes fowleri | + | + | + | [58,67,69,141] | |||||
| Aedes furcifer | + | [172] | |||||||
| Aedes gibinsis | + | [139,171] | |||||||
| Aedes haworthi | + | [10] | |||||||
| Aedes ingrani | + | [10] | |||||||
| Aedes irritans | + | [159] | |||||||
| Aedes longipalpis | + | + | [10,172] | ||||||
| Aedes luteocephalus | + | [10,68,141] | |||||||
| Aedes mcintoshi | + | + | [58,141] | ||||||
| Aedes metallicus | + | [40,139] | |||||||
| Aedes minutus | + | [172] | |||||||
| Aedes mixtus | + | [141] | |||||||
| Aedes mucidus | + | [58] | |||||||
| Aedes multiplex | + | [69] | |||||||
| Aedes nigricephalus | + | [159] | |||||||
| Aedes notoscriptus | + | [69] | |||||||
| Aedes ochraceus | + | [58] | |||||||
| Aedes opok | + | [10,67] | |||||||
| Aedes palpalis | + | [10,53,67] | |||||||
| Aedes polynesiensis | + | [69] | |||||||
| Aedes procax | + | [69] | |||||||
| Aedes simpsoni | + | + | + | [10,20,40,53,57,68,96,103,118,139,141,145,170] | |||||
| Aedes simulans | + | [177] | |||||||
| Aedes soleatus | + | [40,139] | |||||||
| Aedes stockesi | + | [10] | |||||||
| Aedes subargenteopunctatus | + | [10] | |||||||
| Aedes tarsalis | + | [10,40,67,139,154,171] | |||||||
| Aedes vexans | + | + | + | [69,149,157,172] | |||||
| Aedes vigilax | + | + | [69,172] | ||||||
| Aedes vittatus | + | + | + | + | [10,58,118,137,141,145,154,172] | ||||
| Aedes vittiger | + | [69] | |||||||
| Aedes wellmani | + | [40,139] | |||||||
| Anopheles annuliotus | + | [10,171] | |||||||
| Anopheles annulipes | + | [69] | |||||||
| Anopheles arabiensis | + | + | + | + | [69,178,179,180] | ||||
| Anopheles ardensis | + | + | + | [178] | |||||
| Anopheles argenteolobatus | + | + | [178] | ||||||
| Anopheles austenii | + | + | [178] | ||||||
| Anopheles azevedoi | + | [178] | |||||||
| Anopheles bambusae | + | [10] | |||||||
| Anopheles barberellus | + | + | + | [178] | |||||
| Anopheles berghei | + | [178] | |||||||
| Anopheles bervoetsi | + | + | + | [178] | |||||
| Anopheles brohieri | + | + | + | [178] | |||||
| Anopheles brunnipe | + | + | + | + | + | [178,181] | |||
| Anopheles buxtoni | + | [178] | |||||||
| Anopheles caliginosus | + | + | [178] | ||||||
| Anopheles carnevalei | + | + | + | [178,182,183] | |||||
| Anopheles caroni | + | + | [178] | ||||||
| Anopheles christyi | + | + | + | [178] | |||||
| Anopheles cinctus | + | + | + | + | + | + | + | [141,178] | |
| Anopheles cinerus | + | + | [178] | ||||||
| Anopheles claviger | + | [69] | |||||||
| Anopheles coluzzii | + | + | + | + | + | + | + | + | [69,149,157,178,180] |
| Anopheles confusus | + | [178] | |||||||
| Anopheles cconcolor | + | + | + | [178,180] | |||||
| Anopheles coustani | + | + | + | + | + | + | + | [10,69,141,178,179,180] | |
| Anopheles cydippis | + | + | + | + | + | [178] | |||
| Anopheles dureni | + | + | + | [178] | |||||
| Anopheles deemingi | + | [178] | |||||||
| Anopheles demeilloni | + | + | + | + | [178] | ||||
| Anopheles distinctus | + | + | [178] | ||||||
| Anopheles dthali | + | [178] | |||||||
| Anopheles domicolus | + | + | + | [178] | |||||
| Anopheles dualaensis | + | [178] | |||||||
| Anopheles eouzani | + | + | [178] | ||||||
| Anopheles faini | + | + | [177,178] | ||||||
| Anopheles flavicosta | + | + | + | [141,178] | |||||
| Anopheles freetownensis | + | + | + | [178] | |||||
| Anopheles fontenillei | + | [178] | |||||||
| Anopheles fuscivenosus | + | [178] | |||||||
| Anopheles funestus | + | + | + | + | + | + | + | + | [10,33,67,69,141,146,154,177,178,179,180,181,182] |
| Anopheles gambiae | + | + | + | + | + | + | + | + | [10,20,32,33,67,69,96,102,109,111,118,137,139,141,142,143,144,145,146,147,150,154,157,159,164,170,178,179,180,181,182,184] |
| Anopheles garnhami | + | [178] | |||||||
| Anopheles gabonensis | + | [178] | |||||||
| Anopheles gibbinsi | + | + | + | [178] | |||||
| Anopheles hamoni | + | [178] | |||||||
| Anopheles hancocki | + | + | + | + | [178,181] | ||||
| Anopheles hargreavesi | + | + | + | + | + | [178] | |||
| Anopheles charperi | + | [178] | |||||||
| Anopheles hyrcanus | + | [69] | |||||||
| Anopheles implexus | + | + | + | + | + | + | [10,178,183] | ||
| Anopheles jebudensis | + | + | + | + | + | [177,178] | |||
| Anopheles keniensis | + | [178] | |||||||
| Anopheles kingi | + | [178] | |||||||
| Anopheles leesoni | + | + | + | + | + | + | [178] | ||
| Anopheles listeri | + | [178,180] | |||||||
| Anopheles lloreti | + | [178] | |||||||
| Anopheles longipalpis | + | + | + | + | [178] | ||||
| Anopheles maculipalpis | + | + | + | + | + | [178] | |||
| Anopheles maculipennis | + | + | [69,171] | ||||||
| Anopheles marshallii | + | + | + | + | + | + | [176,177,178,183] | ||
| Anopheles melas | + | + | + | + | + | + | + | [32,49,167,178,180,182] | |
| Anopheles meraukensis | + | [172] | |||||||
| Anopheles millecampsi | + | [178] | |||||||
| Anopheles mortiauxi | + | [178] | |||||||
| Anopheles moucheti | + | + | + | + | + | + | [147,149,170,178,181,182,183] | ||
| Anopheles mousinhoi | + | + | [178] | ||||||
| Anopheles multicinctus | + | + | + | [178] | |||||
| Anopheles namibiensis | + | [178] | |||||||
| Anopheles natalensis | + | + | + | + | + | + | [10,177,178] | ||
| Anopheles nili | + | + | + | + | + | + | + | + | [10,141,170,177,178,179,180,181,182] |
| Anopheles njombiensis | + | + | [178] | ||||||
| Anopheles obscurus | + | + | + | + | + | + | + | [176,178] | |
| Anopheles okuensis | + | [178] | |||||||
| Anopheles ovengensis | + | + | [178,182] | ||||||
| Anopheles paludis | + | + | + | + | + | + | [10,170,172,176,178,181] | ||
| Anopheles pharaoensis | + | + | + | + | + | + | [69,109,141,178,179,180] | ||
| Anopheles pretoriensis | + | + | + | + | + | + | [178] | ||
| Anopheles rageaui | + | + | + | [178] | |||||
| Anopheles rhodesiensis | + | + | + | + | + | + | [178] | ||
| Anopheles rodhaini | + | [178] | |||||||
| Anopheles rivulorum | + | + | + | + | [178] | ||||
| Anopheles rivulorum-like | + | [178] | |||||||
| Anopheles ruarinus | + | [178,180] | |||||||
| Anopheles rufipes | + | + | + | + | + | + | + | [67,141,178,179,180] | |
| Anopheles schwetzi | + | + | + | [177,178] | |||||
| Anopheles seydeli | + | [178] | |||||||
| Anopheles smithii | + | + | + | + | [177,178] | ||||
| Anopheles somalicus | + | [178] | |||||||
| Anopheles squamosus | + | + | + | + | + | + | + | [58,178,179,180] | |
| Anopheles symesi | + | [178] | |||||||
| Anopheles tchekedii | + | [178] | |||||||
| Anopheles tenebrosus | + | + | + | + | + | + | [147,149,157,176,178] | ||
| Anopheles theileri | + | + | + | [178] | |||||
| Anopheles vanhoofi | + | + | [178] | ||||||
| Anopheles versus | + | [177] | |||||||
| Anopheles vinckei | + | [33,178] | |||||||
| Anopheles walravensi | + | + | [178] | ||||||
| Anopheles wellecomei | + | + | + | + | + | + | [69,178,179] | ||
| Anopheles ziemmani | + | + | + | + | + | + | + | [67,141,147,178,179,180] | |
| Anopheles zombaensi | + | [177] | |||||||
| Coquelettidia spp. | + | + | [10,141,170] | ||||||
| Coquelettidia annettii | + | [139] | |||||||
| Coquelettidia aurites | + | [176,177] | |||||||
| Coquelettidia cristata | + | [10] | |||||||
| Coquelettidia fraseri | + | [10] | |||||||
| Coquelettidia maculipennis | + | [40,139] | |||||||
| Coquelettidia microannulata | + | [177] | |||||||
| Coquelettidia pseudoconopas | + | + | [10,177] | ||||||
| Coquelettidia richiardii | + | [69] | |||||||
| Coquelettidia versicor | + | [177] | |||||||
| Coquelettidia xanthogaster | + | [69] | |||||||
| Culex albiventis | + | + | [40,185] | ||||||
| Culex annulioris | + | + | + | [10,40,141,172,177,178] | |||||
| Culex annulirostries | + | [69] | |||||||
| Culex antenatus | + | + | [40,58,145,150,159,185] | ||||||
| Culex andersoni | + | [177] | |||||||
| Culex argenteopunctatus | + | [185] | |||||||
| Culex australicus | + | [69] | |||||||
| Culex cinerus | + | + | + | [141,142,159,176,177,185] | |||||
| Culex cinerellus | + | + | + | [111,159,177,185] | |||||
| Culex duttoni | + | + | + | [40,53,67,95,111,139,145,147,149,150,154,157,170,171,172,185,186,187] | |||||
| Culex decens | + | + | [67,95,109,111,142,145,146,150,157,159,176] | ||||||
| Culex eouzani | + | [185] | |||||||
| Culex fatigans | + | [188] | |||||||
| Culex guiarti | + | [185] | |||||||
| Culex horridus | + | [185] | |||||||
| Culex individiosus | + | [139] | |||||||
| Culex insignis | + | + | [69,185] | ||||||
| Culex macfie | + | [185] | |||||||
| Culex muspratti | + | [185] | |||||||
| Culex musarum | + | [185] | |||||||
| Culex simpliciforceps | + | [185] | |||||||
| Culex moucheti | + | [40,53,139,170,171,185] | |||||||
| Culex modestus | + | [69] | |||||||
| Culex molestus | + | [69] | |||||||
| Culex neavei | + | + | + | [67,172,185] | |||||
| Culex nebulosus | + | + | + | [141,159,177,185] | |||||
| Culex orbostiensis | + | [69] | |||||||
| Culex ornothoracic | + | [139,171,185] | |||||||
| Culex perexiguus | + | [69] | |||||||
| Culex perfuscus | + | [139,145,154,170,185] | |||||||
| Culex perfidiosus | + | + | [10,67,141,142,154,170,185] | ||||||
| Culex pipiens | + | + | [69,139,150,186] | ||||||
| Culex phillipi | + | [40,185] | |||||||
| Culex poicilipes | + | + | [10,69,147,150,157] | ||||||
| Culex poecilipes | + | + | [149] | ||||||
| Culex pruina | + | [40,170,185] | |||||||
| Culex quasiguiarti | + | [177] | |||||||
| Culex quinquefasciatus | + | + | + | + | + | + | [10,20,40,53,58,69,95,96,109,111,118,141,142,143,145,146,147,149,157,159,164,167,170,172] | ||
| Culex rubinotus | + | + | [176,177] | ||||||
| Culex rima | + | + | + | [69,159,177,185] | |||||
| Culex sitiens | + | [69] | |||||||
| Culex schwetzi | + | [185] | |||||||
| Culex semibrunneus | + | [177,185] | |||||||
| Culex simpsoni | + | + | [149,150,177] | ||||||
| Culex sunyaniensis | + | [185] | |||||||
| Culex subaequali | + | [185] | |||||||
| Culex tigripes | + | + | + | + | [10,40,53,95,109,137,141,142,145,146,150,154,159,171] | ||||
| Culex trifilatus | + | + | [139,177] | ||||||
| Culex univittatus | + | + | [40,139,150,170,171,177] | ||||||
| Culex watti | + | + | [69,177] | ||||||
| Culex wiggleworthi | + | [40,139,170,171,185] | |||||||
| Culex taufliebi | + | [111] | |||||||
| Culex thalassius | + | [185] | |||||||
| Culex theileri | + | [177] | |||||||
| Culex trifoliatus | + | + | [111,139,185] | ||||||
| Culex umbripes | + | [111,185] | |||||||
| Eretmapodites spp. | + | + | + | + | [40,53,139] | ||||
| Eretmapodites quinquevittatus | + | + | + | [20,95,145] | |||||
| Eretmapodites chrysogaster | + | + | [10,40,139,171,177] | ||||||
| Eretmapodites grahami | + | [177] | |||||||
| Eretmapodites inornatus | + | + | [10,111,172] | ||||||
| Eretmapodites plioleucus | + | [139] | |||||||
| Ficolbia spp. | + | [40] | |||||||
| Ficalbia Flavopicta | + | [139] | |||||||
| Ficalbia malfeyi | + | [176] | |||||||
| Ficalbia mediolineata | + | [159] | |||||||
| Ficalbia uniformis | + | [177] | |||||||
| Finlayas spp. | + | [177] | |||||||
| Lutzia tigripes | + | + | + | + | [40,67,69,111,139,142,170,177,186] | ||||
| Mansona africana | + | + | + | + | [10,20,58,95,96,141,143,159,170,172,176] | ||||
| Mansona uniformis | + | + | + | + | [10,20,58,69,96,102,109,141,143,159,170] | ||||
| Mimmonyia spp. | + | [40] | |||||||
| Mimmonyia flavopicta | + | [139] | |||||||
| Mimmonyia plumosa | + | [177] | |||||||
| Ochlerothatus rusticus | + | [69] | |||||||
| Ochlerothatus excrucians | + | [69] | |||||||
| Orthopodomyia reunionensis | + | [69] | |||||||
| Uranotaenia spp. | + | + | + | [40] | |||||
| Uranotaenia bilineata | + | + | [139,171,176,177] | ||||||
| Uranotaenia cavernicola | + | [176,177] | |||||||
| Uranotaenia nigromaculata | + | [176,177] | |||||||
| Uranotaenia nigripes | + | [177] | |||||||
| Uranotaenia caliginosa | + | [176] | |||||||
| Uranotaenia caeruleocephala | + | [176] | |||||||
| Uranotaenia machadoi | + | [176] | |||||||
| Uranotaenia pallidocephala | + | [176] | |||||||
| Uranotaenia balfoui | + | [176] | |||||||
| Uranotaenia chorleyi | + | [176] | |||||||
| Uranotaenia alboabdominalis | + | [176] | |||||||
| Uranotaenia mashonaensis | + | + | [139,159,176,177] | ||||||
| Verralina funerea | + | [69] | |||||||
| Toxorhinchites spp. | + | [40,53] | |||||||
+: presence of species. Ang: Angola; CMR: Cameroon; Cha: Chad; Ga: Gabon; RoC: Republic of the Congo; CAR: Central African Republic; DRC: Democratic Republic of Congo; EG: Equatorial Guinea.
3.7. Arboviruses and Associated Mosquito Vectors in the Central African Region from 1993 to 2023
The spillovers of arboviruses of public health importance have reportedly been associated with mosquito genera of medical and zoonotic importance such as Aedes, Culex, Anopheles, etc. The vector competence of Ae. aegypti [139,189,190,191] and Ae. albopictus [190,192] in the transmission of major arboviruses has been well-documented. The vector competence and the association of some Aedes spp. as potential vectors of important viruses is still unknown. For the Culex mosquitoes already identified in studies of the Central African region, they have been frequently reported to be associated with zoonotic arboviruses such as RVFV and WNV [19,188,191,193]. Of the Culex spp. involved in the spread of zoonotic arboviruses, Culex pipiens was reported to be associated with CHIKV [19,193,194,195]. The genus Anopheles has also been reported to be associated with some medically important arboviruses, such as YFV (An. gambiae s.l.) [196], ZIKV (An. moucheti) [194], and CHIKV (An. funestus) [191]. Moreover, other mosquitoes that are not well-known in the region are associated with arboviruses and include Eretmapodites spp. (ZIKV) [40,191], Coquelettidia spp. (YFV and RVFV) [189,191,193], and Lutzia tigripes, which was reported to be potential vector of YFV, DENV, and WNFV [139,176,191]. The occurrence of these competent and potential vectors in the different countries of this subregion of Africa could indicate the risk of spillover of the different viruses highlighted in Table 3. Therefore, vector competence studies and transmission studies, together with clinical diagnosis, are required in order to keep track of the patterns of occurrence and spread of arboviruses of medical and zoonotic importance in the Central African region.
Table 3.
Arboviruses and associated mosquito vectors in the Central African region from January 1993 to June 2023.
Table 3.
Arboviruses and associated mosquito vectors in the Central African region from January 1993 to June 2023.
| Species | Virus | Diagnosis | References |
|---|---|---|---|
| Ae. aegypti | DENV | V, V, V, P, V | [190,191,197,198,199] |
| CHIK | V, V, V, P, V, V, P + V, V, V | [190,191,197,198,199,200,201,202,203] | |
| ZIKV | V, V, V, V, P, V | [149,190,191,197,198,199] | |
| YFV | V, V, V, P, V, V | [190,191,197,198,199,204] | |
| RVFV | V + P, V, V, V | [59,191,193,199] | |
| WNV | P | [194] | |
| Ross River | V, V, V, P | [190,191,197,198] | |
| Murray Vallée | V, V, V, P | [190,191,197,198] | |
| Wesselsbron | V, V, V + P | [191,200,201] | |
| Bakanki | V, P | [191,205] | |
| O’nyong Nyong | V, V | [191,202] | |
| Ae. africanus | CHIKV | V, V, V, V, V | [67,191,204,206] |
| WNV | V, V, P, V | [67,191,205,206] | |
| YFV | V, V, V, V | [67,191,204,206] | |
| ZIKV | V, V, P, V, V, V | [67,148,172,191,204,206] | |
| RVFV | V, V, P | [67,204,205] | |
| Bozo | V, P | [67,205] | |
| Bouboui | V, V, P, V | [67,172,191,206] | |
| Babanki | V, V, P + V | [191,200,201] | |
| Uganda | V, V, P + V, V | [191,200,201,206] | |
| Wesselsbron | V, V, V, P + V | [67,191,200,201] | |
| Orungo | V, P | [67,205] | |
| Middelburg | V, V, P + V | [191,200,201] | |
| Saboya | P, P | [172,205] | |
| Semliki | V, V, P + V, V | [191,200,201,206] | |
| Yaoundé | V, V, P + V, P | [191,200,201,205] | |
| Ae. albopictus | DENV | V, P, V, V, V, P | [29,96,190,192,197,198] |
| CHIKV | P, V, V, V, P, V + P | [96,190,191,197,198,201] | |
| ZIKV | V, V, V, V, P | [148,190,191,197,198] | |
| YFV | V, V, V, P | [190,191,197,198] | |
| RVFV | V + P | [58] | |
| WNV | V, V, P, V | [190,191,197,198] | |
| Usutu | V, V, P + V | [191,200,201] | |
| Ross River | V, V, P | [190,198,198] | |
| Murray Vallée | V, V, P | [190,197,198] | |
| Ae. argenteopunctatus | CHIKV | V, V, P | [191,200,201] |
| YFV | V, V, P | [190,197,198] | |
| ZIKV | V, V, P | [190,197,198] | |
| Semliki | V, V, V, P | [190,194,197,198] | |
| Kedougou | V | [194] | |
| Simbu | V | [194] | |
| Ae. bromelia | YFV | V | [204] |
| Ae. caballus | RVFV | V, V, P, V | [190,197,198,207] |
| Midelburg | V, V, P + V | [191,200,201] | |
| Wesselsbron | V, V, V + P, V | [191,200,201,204] | |
| Ae. cordeleri | CHIKV | V, V, V + P | [191,200,201] |
| Ae. caspiua | Usutu | V, V, P + V | [191,200,201] |
| Ae. circumluteocus | RVFV | V, V, V + P, V, V | [58,191,193,200,201] |
| Wesselsbron | V, V, P + V | [191,200,201] | |
| Pongola | V, V, P + V | [191,200,201] | |
| Bunyamwera | V, V, P + V | [191,200,201] | |
| Ndumu | V, V, P + V | [191,200,201] | |
| Spondweni | P, V, V, P + V | [172,198,199,200] | |
| Ae. cumminsii | RVFV | V, V, P + V, V | [189,200,201,208] |
| Spondweni | V, V, P + V | [191,200,201] | |
| Ae. dalzieli | CHIKV | V, V | [194,209] |
| ZIKV | P, V, V, V, P + V | [172,191,194,200,201] | |
| RVFV | V + P, P | [58,181,210] | |
| Middelburg | V | [194] | |
| Ndumu | V | [194] | |
| Kedougou | V, V, P, P, V | [172,191,194,200,201] | |
| Wesselsbron | P, V | [172,194] | |
| Bunyawera | V | [194] | |
| Shokwe | V | [194] | |
| Simbu | V | [194] | |
| Pongola | V | [194] | |
| Zinga | V | [194] | |
| Ae. dentatus | YFV | V | [194] |
| RVFV | V, V, P + V | [191,200,201] | |
| Ae. domesticus | Bunyamwera | V, V, V | [139,199,203] |
| Ae. furcifer/taylori | CHIKV | V, V, V, P + V, V, V | [189,191,200,201,209,211] |
| ZIKV | P, V, V, V, V + P | [172,191,194,200,201] | |
| YFV | V, V, V, V + P | [191,194,200,201] | |
| Bunyamwera | V | [194] | |
| Bouboui | V | [194] | |
| Bwamba | V, V, P + V | [191,200,201] | |
| Ae. longipalpis | Uganda | P, V, V, P + V | [172,191,200,201] |
| Ae. luteocephalus | CHIKV | V, V, V, V, V + P, V | [189,191,194,200,201,204] |
| YFV | V, V, V, V + P, V, V | [191,194,200,201,204,209] | |
| ZIKV | V, V, V + P, V | [191,194,200,201] | |
| DENV | V, V | [194,204] | |
| Bunnyamwera | V, V, P + V | [191,200,201] | |
| Ae. mcintoshi | CHIKV | V, V, P + V | [191,200,201] |
| RVFV | V + P, V | [58,193] | |
| Ndumu | V, V, V + P, V | [191,200,201] | |
| Pongola | V, V, V + P | [191,200,201] | |
| Wesselsbron | V, V, V + P | [191,200,201] | |
| Babanki | V, V, V, V + P | [191,192,200,201] | |
| Ngari | V, V, P + V | [191,200,201] | |
| Bunyamwera | V, V, P + V | [191,200,201] | |
| Ae. metallicus | YFV | V, V, V, V + P, V, P | [189,191,200,201,204,205] |
| ZIKV | V, V, V + P, P | [191,200,201,205] | |
| Ae. minutus | ZIKV | V, V, P + V | [191,200,201] |
| Ndumu | V | [194] | |
| Kedougou | P, V, V, V, V + P | [172,191,194,200,201] | |
| Wesselsbron | V | [194] | |
| Ae. neoafricanus | CHIKV | V, V, P + V | [191,200,201] |
| YFV | V, V | [194,204] | |
| Ae. ochraceus | RVFV | V + P | [58] |
| Ndumu | V, V, P + V | [191,200,201] | |
| Babanki | V, V, P + V | [191,200,201] | |
| Ae. opok | CHIKV | V, V, V | [67,189,206] |
| YFV | V, V, V, V + P, V, V | [67,191,200,201,204,206] | |
| WNV | P | [205] | |
| ZIKV | V, V, V, V + P, V, V | [67,191,200,201,204,206] | |
| Bouboui | V, V | [67,204] | |
| Orungo | V | [67] | |
| Wesselbron | V | [67] | |
| Bozo | V | [67] | |
| Middelburg | P | [205] | |
| Saboya | P | [205] | |
| Semeniki | V | [206] | |
| Yaoundé | P | [205] | |
| Ae. palpalis | RVFV | V, V | [67,193] |
| Middelburg | V, V | [199,212] | |
| Simbu | V | [67] | |
| Ae. simpsoni | CHIKV | V, V, P | [139,189,205] |
| YFV | V, V, V + P, V | [191,200,201,204] | |
| Babanki | V, V, V + P | [191,200,201] | |
| Ngari | V, V, V + P | [191,200,201] | |
| Ae. tarsalis | ZIKV | V, V, V + P | [191,200,201] |
| Pata | V | [67] | |
| Bunyamwera | V | [212] | |
| Middelburg | V | [212] | |
| Pangola | V | [67] | |
| Kedougou | V, V, V, V + P | [67,191,200,201] | |
| Wesselbron | V | [67] | |
| Ae. tricholabic | Ndumu | V, V, V + P | [191,200,201] |
| Pongola | P | [192] | |
| Bunyamwera | P | [212] | |
| Ngari | P | [212] | |
| Ae. vexans | wesselsbron | P | [172] |
| Ae. vigilax | Edge | P | [172] |
| Ae. vittatus | CHIKV | V, V, V, V + P | [189,191,200,201] |
| RVFV | V + P | [58] | |
| YFV | V, V, V, V + P | [191,194,200,201] | |
| ZIKV | V, V, V, V + P | [191,194,200,201] | |
| Sindbis | V | [194] | |
| Middelburg | V | [67] | |
| Semliki | V | [194] | |
| Wesselsbron | V | [67] | |
| Bunyamuera | V | [194] | |
| Simbu | V, V | [67,194] | |
| Pongola | V | [194] | |
| Saboya | P | [172] | |
| Ae. abnormalis, Ae. alternans, Ae. australis, Ae. cinerus, Ae. centropunctatus, Ae. contigus, Ae. dendrophillus, Ae. dufouri, Ae. fraseri, Ae. flavifrons, Ae. fowleri, Ae. gibinsis, Ae. haworth, Ae. ingrani, Ae. irritans, Ae. mixtus, Ae. mucidus, Ae. multiplex, Ae. simulans, Ae. soleatus, Ae. stockesi Ae. polynesiensis, Ae. procax Ae. nigricephalus, Ae. notoscriptus, Ae. vittiger, Ae. subargenteopunctatus, Ae. wellmani | n.a | / | |
| An. brohieri | Sindbis | V | [194] |
| An. coustani | CHIKV | V | [194] |
| Bwanba | V, V, V + P | [191,200,201] | |
| An. funestus | CHIKV | V, V, V, V + P | [191,194,200,201] |
| WNV | V | [67] | |
| Nyando | V, V, V + P, V | [67,191,200,201] | |
| Ngari | V, V, V + P | [191,200,201] | |
| Bwamba | V, V, V + P, V | [67,191,200,201] | |
| Bunyamwera | V, V, V + P | [191,200,201] | |
| O’nyong Nyong | V, V, V + P | [191,200,201] | |
| Pongola | V | [194] | |
| Tataguine | V | [67] | |
| An. gambiae | YFV | V, V, V, V, V + P, V | [139,189,191,200,201,204,212] |
| ZIKV | V, V, V + P | [191,200,201] | |
| Ilesha | V, V, V, V + P | [67,191,200,201] | |
| Bwamba | V, V, V, V + P | [191,194,200,201] | |
| O’nyong Nyong | V, V, V + P | [191,200,201] | |
| Middelburg | V, V | [67,213] | |
| Tataguine | V | [194] | |
| Orungo | V | [67] | |
| Ae. mercaukensis | Edge | P | [172] |
| An. moucheti | ZIKV | V | [194] |
| An. nili | Tataguine | V | [194] |
| An. paludis | Bouboui | P, V, V, V + P | [172,191,200,201] |
| An. maculipennis | Usutu | V, V, V + P | [191,200,201] |
| An. annuliotus, An. annulipes, An. arabiensis, An. ardensis, An. argenteolobatus, An. austenii, An. azevedoi, An. bambusae, An. barberellus, An. berghei, An. bervoetsi, An. brunnipe, An. buxtoni, An. caliginosus, An. carnevalei, An. caroni, An. christyi, An. cinctus, An. cinerus, An. claviger, An. coluzzii, An. confusus, An. concolor, An. cydippis, An. dureni, An. deemingi, An. demeilloni, An. distinctus, An. dthali, An. domicolus, An. dualaensis, An. eouzani, An. faini, An. flavicosta, An. freetownensis, An. fontenillei, An. fuscivenosus, An. garnhami, An. gabonensis, An. gibbinsi, An. hamoni, An. hancocki, An. hargreavesi, An. harperi;An. hyrcanus, An. jebudensis, An. keniensis, An. kingi, An. leesoni, An. listeri, An. lloreti, An. longipalpis, An. maculipalpis, An. marshallii, An. melas, An. millecampsi, An. mortiauxi, An. mousinhoi, An. multicinctus, An. namibiensis, An. natalensis, An. njombiensis, An. obscurus, An. okuensis, An. ovengensis, An. pharaoensis, An. pretoriensis, An. rageaui, An. rhodesiensis, An. rodhaini, An. rivulorum, An. rivulorum-like, An. ruarinus, An. rufipes, An. schwetzi, An. seydeli, An. smithii, An. somalicus, An. squamosus, An. symesi, An. tchekedii, An. tenebrosus, An. theileri, An. vanhoofi, An. versus, An. vinckei, An. walravensi, An. wellecomei, An. ziemmani, An. zombaensis | n.a | / | / |
| Coquelettidia. spp. | YFV | V | [189] |
| Co. fuscopennata | Sindbis | V, V, V, V + P | [191,194,200,201] |
| Co. aurites | Usutu | V, V, V + P | [191,200,201] |
| Co. annettii, Co. cristata, Co. fraseri, Co. maculipennis, Co. microannulata, Co. pseudoconopas, Co. richiardii, Co. versicor, Co. xanthogaster | n.a | / | |
| Cx. albiventis | Arumowot | V, V, V + P | [191,200,201] |
| Ntaya | V | [212] | |
| Cx. annulirostris | Kamese | P, V, V, V + P | [172,191,200,201] |
| Edge | P | [172] | |
| Cx. antenatus | RVFV | V + P, V | [58,193] |
| Arumowat | V | [191,200,201] | |
| Cx. cinerus | M’Poko | V | [194,212] |
| Ntaya | V | [212] | |
| Cx. duttoni | Uganda | P | [172] |
| wesselsbron | P | [172] | |
| Cx. decens | Sindbis | V | [194] |
| Usutu | V | [194] | |
| Kamese | V | [67] | |
| Nyando | V | [67] | |
| Cx. individiosus | Sindbis | V | [194] |
| Cx. ingrani | Bagaza | V, V, V + P | [191,200,201] |
| Cx. moucheti | Ntaya | V, V | [139,212] |
| Cx. modestus | WNV | V, V, V + P | [191,200,201] |
| Cx. neavei | WNV | V, V, V + P | [191,200,201,209] |
| Usutu | V, V, V + P | [191,200,201] | |
| Sindbis | V, V, V + P | [191,200,201] | |
| Spondweni | P, V, V, V + P | [172,191,200,201] | |
| Cx. nebulosus | Yaoundé | V, V, V + P | [191,200,201] |
| Ntaya | V | [212] | |
| Cx. perfuscus | WNV | V, V | [67,194] |
| Sindbis | V | [194] | |
| Bagaza | V, V, V, V + P | [67,191,200,201] | |
| Usutu | V, V, V, V + P | [191,194,200,201] | |
| M’Poko | V | [67] | |
| Wesselsbron | V | [67] | |
| Cx. pipiens | CHIKV | V | [194] |
| RVFV | V | [193] | |
| WNV | V, P, V, V + P | [191,195,200,201] | |
| Sindbis | P, V, V, V + P | [191,194,200,201] | |
| Babanki | P | [194] | |
| Usutu | V, V, V + P | [191,200,201] | |
| Cx. poicilipes | WNV | V | [209] |
| Cx. pruina | Kamese | V, V, V, V + P | [67,191,200,201] |
| Bozo | V | [67] | |
| Cx. quinquefasciatus | WNV | V, V, V + P | [191,200,201] |
| RVFV | V + P, V | [58,193] | |
| Wesselsbron | V, V, V + P | [191,200,201] | |
| Cx. rubinotus | Banzi | P, V, V, V + P | [172,191,200,201] |
| arumowat | V, V, V + P | [191,200,201] | |
| WNV | P | [192] | |
| Yaoundé | P | [192] | |
| Ndumu | P | [192] | |
| Cx. univittatus | WNV | V, V, V + P | [139,192,212] |
| Sindbis | V, V, V + P | [191,200,201] | |
| Wesselsbron | V, V, V + P | [191,200,201] | |
| Usutu | V, V, V + P | [191,200,201] | |
| Spondweni | V, V, V + P | [191,200,201] | |
| Ndumu | V, V, V + P | [191,200,201] | |
| Bagaza | V, V, V + P | [191,200,201] | |
| Cx. tarsalis | WNV | P | [195] |
| Cx. telesilila | Sindbis | V | [194] |
| Ntaya | V | [212] | |
| Cx. theileri | WNV | V, V, V + P | [191,200,201] |
| Cx. tigripes | Ntaya | V | [212] |
| Mossuril | V | [67] | |
| Cx. annulirostries, Cx. andersoni, Cx. argenteopunctatus, Cx. australicus, Cx. cinerellus, Cx. eouzani, Cx. fatigans, Cx. guiarti, Cx. horridus, Cx. insignis, Cx. macfiei, Cx. muspratti, Cx. musarum, Cx. simpliciforceps, Cx. molestus, Cx. nebulosus, Cx. orbostiensis, Cx. ornothoracic, Cx. perexiguus, Cx. perfidiosus, Cx. phillipi, Cx. poecilipes, Cx. quasiguiarti, Cx. rima, Cx. sitiens, Cx. schwetzi, Cx. semibrunerus, Cx. simpsoni Cx. sunyaniensis, Cx. subaequalis, Cx. watti, Cx. wiggleworthi, Cx. taufliebi, Cx. thalassius, Cx. trifoliatus, Cx. umbripes | n.a | / | / |
| Er. quinquevittatus | ZIKV | V, V, V + P | [191,200,201] |
| Er. chrysogaster | Spondweni | V | [212] |
| Middelburg | V | [212] | |
| Er. inornatus | ZIKV | V, V, V, V + P | [172,191,200,201] |
| Middellburg | V | [212] | |
| Bouboui | P | [172] | |
| Er. grahami, Er. pliol | n.a | / | |
| Ficolbia. spp., Fi. Flavopicta, Fi. malfeyi, Fi. mediolineata, Fi. uniformis | n.a | / | |
| Finlayas. spp. | n.a | / | |
| Lu. tiggipes | n.a | / | |
| Ma. africana | RVFV | V + P | [58] |
| Middelburg | V, V, V, V, V + P | [67,191,200,201,212] | |
| Wesselsbron | V, V, V + P | [191,200,201] | |
| Spondweni | V, V, V + P | [191,200,201] | |
| Banzi | P | [172] | |
| Ma. uniformis | ZIKV | V, V, V, V + P | [191,194,200,201] |
| Spondweni | V, V, V + P | [191,200,201] | |
| Bwamba | V, V, V + P | [191,200,201] | |
| O’nyong Nyong | V, V, V + P | [191,200,201] | |
| Ndumu | V, V, V + P | [191,200,201] | |
| Mimmonyia. spp., Mi. flavopicta, Mi. plumosa | n.a | / | / |
| Oc. rusticus, Oc. excrucians | n.a | / | / |
| Or. reunionensis | n.a | / | / |
| Ur. machadoi | n.a | / | / |
| Uranotaenia. spp., Ur. bilineata, Ur. cavernicola, Ur. cavernicola, Ur. nigripes, Ur. caliginosa, Ur. caeruleocephala, Ur. pallidocephala, Ur. balfoui, Ur. chorleyi, Ur. alboabdominali, Ur. mashonaensis | n.a | / | / |
| Ve. funerea | n.a | / | / |
| Toxorhinchite. spp. | Mosuril | V, V | [67,213] |
| Kamese | V, V | [67,213] |
Not available (n.a); virus isolation (v); P: RNA detection; virus isolation and RNA detection in the same study (V + P). The order of techniques used to show the vector transmission potential of the different mosquito species separated by commas in the diagnosis column refers and/or corresponds to the references in the square brackets of the references column.
Of the 164 eligible papers on the topic used in this study, we found that most of them were from Cameroon, followed by Gabon, and the fewest were recorded in Equatorial Guinea. The most commonly reported arboviruses to cause epidemics were chikungunya and dengue. The entomological records showed >248 species of mosquitoes in different studies related to arboviruses and regrouped under 15 genera, with Anopheles (n = 100 species), Culex (n = 56 species), and Aedes (n = 52 species) having the highest species diversity and broadest distribution. Three genera were rarely represented (with only one species), and these included Orthopodomyia, Lutzia, and Verrallina, but individuals of the genera Toxorhinchites and Finlayas were not identified up to the species level. We found that these two Aedes species were involved in major epidemics of the six medically important arboviruses, and other rare mosquito genera consisted of competent species and were associated with outbreaks of zoonotic arboviruses. These findings revealed the existing gaps in the epidemiological and entomological data of the various countries of the central African region, and the need for regular surveillance at the country level. There is need to focus research on arbovirus ecology and to establish the vector competence of other frequently identified mosquitoes in the region. Although few regional studies were documented, there is still a need to conduct a multicountry project on arboviruses and vectors in Central Africa in order to propose sustainable control measures.
4. Conclusions
The present study shows that the wide spread of competent mosquito vectors could lead to the spillover of medically important arboviruses in the region, presumably via the free movement of animals and people via porous borders. Although epidemiological studies were found, they were not regularly documented, and this also applies to vector competence and transmission studies. Future studies will consider raw data from technical, scientific, and administrative reports/archives (Ministry of Scientific Research and Ministry of Health) and unpublished information in dissertations (research institutions and Universities) that could enable the study to be more complete. A regional project, organised by the authors of this current work, entitled “Ecology of Arboviruses” (EcoVir) is underway in three countries (Gabon, Benin, and Cote d’Ivoire) to generate more comprehensive epidemiological and entomological field data on this topic.
Author Contributions
Conceptualization, N.P., S.L.S., P.M., R.O. and R.M.N.; methodology: S.L.S.; Validation S.L.S. and R.M.N.; resources, S.C.B., J.Z.B.Z., G.D.M., L.S.D., S.B., A.A.A. and J.F.M.; literature search, N.P. and S.L.S.; data curation, S.L.S.; writing—original draft preparation, N.P. and S.L.S.; writing—review and editing, N.P., S.L.S. and R.M.N.; visualization, S.L.S., N.P., A.A.K., C.R.Z.K. and R.M.N.; supervision, R.M.N. All authors have read and agreed to the published version of the manuscript.
Funding
This work received funding from the EcoVir-DFG project.
Informed Consent Statement
Not applicable.
Data Availability Statement
Available upon request from the corresponding author.
Acknowledgments
We are grateful to authors for making their information available online and for providing information upon request. We are thankful to the Laboratoire d’Ecologie des Maladies Transmissibles (LEMAT) of the Université Libreville Nord for their constructive ideas on how to generate relevant information for this study. This study was conducted as part of the DFG-EcoVir project, and the first author will benefit from it in the finalisation of her PhD thesis.
Conflicts of Interest
The authors declare no conflict of interest.
References
- Maurice, Y. Premières constatations sérologiques sur l’incidence de la maladie de Wesselsbron et de la Fièvre de la Vallée du Rift chez les ovins et les ruminants sauvages du Tchad et du Cameroun. Rev. Elev. Méd. Vét. Pays Trop. 1967, 20, 395–405. [Google Scholar] [CrossRef]
- Durand, J.P.; Bouloy, M.; Richecoeur, L.; Peyrefitte, C.N.; Tolou, H. Rift Valley Fever Virus Infection among French Troops in Chad. Emerg. Infect. Dis. 2003, 9, 751–752. [Google Scholar] [CrossRef] [PubMed]
- Willcox, A.C.; Matthew, H.; Collins, M.H.; Jadi, R.; Keeler, C.; Parr, J.B.; Mumba, D.; Kashamuka, M.; Tshefu, A.; de Silva, A.M.; et al. Seroepidemiology of Dengue, Zika, and Yellow Fever Viruses among Children in the Democratic Republic of the Congo. Am. J. Trop. Med. Hyg. 2018, 99, 756–763. [Google Scholar] [CrossRef] [PubMed]
- ECDC. Assessing the Yellow Fever Outbreak in Angola—European Medical Corps Mission Undertaken in the Framework of the European Union Civil Protection Mechanism. European Centre for Disease Prevention and Control, 10–20 May 2016; ECDC: Stockholm, Sweden, 2016; Volume 7.
- WHO. Winning the War against Yellow Fever, Angola. 2016. Available online: https://www.who.int/news-room/feature-stories/detail/winning-the-war-against-yellow-fever (accessed on 30 May 2023).
- Mbanzulu, K.M.; Mboera, L.E.G.; Luzolo, F.K.; Wumba, R.; Misinzo, G.; Kimera, S.I. Mosquito-borne viral diseases in the Democratic Republic of the Congo: A review. Parasites Vectors 2020, 13, 103. [Google Scholar] [CrossRef]
- Ingelbeen, B.; Weregemere, N.A.; Noel, H.; Tshapenda, G.P.; Mossoko, M.; Nsio, J.; Ronsse, A.; Ahuka-Mundeke, S.; Cohuet, S.; Kebela, B. Urban yellow fever outbreak—Democratic Republic of the Congo, 2016: Towards more rapid case detection. PLoS Negl. Trop. Dis. 2018, 12, e0007029. [Google Scholar] [CrossRef]
- De Weggheleire, A.; Nkuba-Ndaye, A.; Mbala-Kingebeni, P.; Mariën, J.; Kindombe-Luzolo, E.; Ilombe, G.; Mangala-Sonzi, D.; Binene-Mbuka, G.; De Smet, B.; Vogt, F.; et al. A Multidisciplinary Investigation of the First Chikungunya Virus Outbreak in Matadi in the Democratic Republic of the Congo. Viruses 2021, 13, 1988. [Google Scholar] [CrossRef]
- Delatte, H.; Paupy, C.; Dehecq, J.S.; Thiria, J.; Failloux, A.B.; Fontenille, D. Aedes albopictus, vecteur des virus du Chikungunya et de la dengue à La Réunion: Biologie et contrôle. Parasite 2008, 15, 3–13. [Google Scholar] [CrossRef]
- Malekani, M.J.; McCollum, A.; Monroe, B.P.; Malekani, V.D.; Mulumba, M.L.; Tshilenge, C.G.; Kondas, A.; Doty, J.B.; Okitolonda, E.W.; Muyembe, J.J.T.; et al. Cas de dengue chez les patients suspects de chikungunya à Kinshasa. Ann. Afr. Med. 2014, 7, 26. [Google Scholar]
- Nur, Y.A.; Groen, J.; Heuvelmans, H.; Tuynman, W.; Copra, C.; Osterhaus, A.D. An outbreak of West Nile fever among migrants in Kisangani, Democratic Republic of Congo. Am. J. Med. Hyg. 1999, 61, 885–888. [Google Scholar] [CrossRef] [PubMed]
- Pastorino, B.; Muyembe-Tamfum, J.J.; Bessaud, M.; Tock, F.; Tolou, H.; Durand, J.P.; Peyrefitte, C.N. Epidemic Resurgence of Chikungunya Virus in Democratic Republic of the Congo: Identification of a New Central African Strain. J. Med. Virol. 2004, 74, 277–282. [Google Scholar] [CrossRef]
- Mariën, J.; Laurent, N.; Gombeer, S.N. First observation of Aedes albopictus in the Tshuapa province (Boende) of the Democratic Republic of the Congo. bioRxiv 2021. [Google Scholar] [CrossRef]
- Tezzo, F.W.; Fasine, S.; Zola, E.M.; Marquetti, M.C.; Mbuka, G.B.; Ilombe, G.; Takasongo, R.M.; Smitz, N.; Bisset, J.A.; Bortel, W.V.; et al. Parasites High Aedes spp. larval indices in Kinshasa, Democratic Republic of Congo. Parasites Vectors 2021, 14, 92. [Google Scholar] [CrossRef] [PubMed]
- Yamamoto, S.P.; Kasamatsu, Y.; Kanbayashi, D.; Kaida, A.; Shirano, M.; Kubo, H.; Goto, T.; Iritani, N. Dengue Virus in Traveler Returning to Japan from the Democratic Republic of the Congo, 2015. Jpn. J. Infect. Dis. 2019, 72, 426–428. [Google Scholar] [CrossRef]
- Proesmans, S.; Katshongo, F.; Milambu, J.; Fungula, B.; Mavoko, H.M.; Ahuka-Mundeke, S.; da Luz, R.I.; Esbroeck, M.V.; Arien, K.K.; Cnops, L.; et al. Dengue and chikungunya among outpatients with acute undifferentiated fever in Kinshasa, Democratic Republic of Congo: A crosssectional study. PLoS Negl. Trop. Dis. 2019, 13, e0007047. [Google Scholar] [CrossRef]
- Amarasinghe, A.; Kuritsky, J.N.; Letson, W.G.; Margolis, S.H. Dengue virus infection in Africa. Emerg. Infect. Dis. 2011, 17, 1349–1353. [Google Scholar] [CrossRef] [PubMed]
- Makiala-Mandanda, S.; Ahuka-Mundeke, S.; Abbate, J.L.; Pukuta-Simbu, E.; Nsio-Mbeta, J.; Berthet, N.; Leroy, E.M.; Becquart, P.; Muyembe-Tamfum, J.J. Identification of dengue and chikungunya cases among suspected cases of yellow fever in the Democratic Republic of the Congo. Vector-Borne Zoonotic Dis. 2018, 18, 364–370. [Google Scholar] [CrossRef]
- Colavita, F.; Vairo, F.; Carletti, F.; Boccardo, C.; Ferraro, F.; Iaiani, G.; Moghazia, S.A.; Galardo, G.; Lalle, E.; Selvaggi, C.; et al. Full-length genome sequence of a dengue serotype 1 virus isolate from a traveler returning from Democratic Republic of Congo to Italy, July 2019. Int. J. Infect. Dis. 2020, 92, 46–48. [Google Scholar] [CrossRef]
- Tshilenge, G. Immunogeno: Protective mechanism for Rift valley fever in the Democratic Republic of Congo. J. Vet. Res. 2012, 79, E1. [Google Scholar] [CrossRef]
- Georges, T.M.; Justin, M.; Victor, M.; Marie, K.J.; Mark, R.; Léopold, M.M.K. Seroprevalence and virus activity of Rift valley fever in cattle in eastern region of Democratic Republic of the Congo. J. Vet. Med. 2018, 2018, 4956378. [Google Scholar] [CrossRef]
- Mbanzulu, K.M.; Mboera, L.E.G.; Wumba, R.; Zanga, J.K.; Luzolo, F.K.; Misinzo, G.; Kimera, S.I. Community Knowledge, Attitude, and Practices Regarding Mosquitoes and Mosquito-Borne Viral Diseases in Kinshasa, Democratic Republic of the Congo. Epidemiologia 2023, 4, 1–17. [Google Scholar] [CrossRef]
- Osterrieth, P.; Deleplanque-Liegeois, P. Présence d’anticorps vis-à-vis de virus transmis par les arthropodes chez le chimpanzé (Pan tronglodites), comparaison de leur état immunitaire à celui de l’homme. Ann. Soc. Belge Méd. Trop. 1961, 1, 63–72. [Google Scholar]
- Sanchez-seco, M.P.; Negredo, A.I.; Puente, S.; Pinazo, M.J.; Shufffenecker, I.; Tenorio, A.; Fedele, C.G.; Domingo, C.; Rubio, J.M.; de Ory, F. Diagnóstico microbiológico del virus chikungunya importado en España (2006–2007): Deteccion de casos en viajeros. Enferm. Infecc. Microbiol. Clin. 2009, 27, 457–461. [Google Scholar] [CrossRef]
- Toto, J.-C.; Abaga, S.; Carnevaley, P.; Simard, F. First report of the oriental mosquito Aedes albopictus on the West African island of Bioko, Equatorial Guinea. Med. Vet. Entomol. 2003, 17, 343–346. [Google Scholar] [CrossRef]
- Leroy, E.M.; Nkoghe, D.; Ollomo, B.; Nze-Nkogue, C.; Becquart, P.; Grard, G.; Pourrut, X.; Charrel, R.; Moureau, G.; Ndjoyi-Mbiguino, A.; et al. Concurrent Chikungunya and Dengue Virus Infections during Simultaneous Outbreaks, Gabon, 2007. Emerg. Infect. Dis. 2009, 15, 591. [Google Scholar] [CrossRef]
- Nkoghe, D.; Kassa Kassa, R.F.; Bisvigou, U.; Caron, M.; Grard, G.; Leroy, E.M. No clinical or biological difference between Chikungunya and Dengue Fever during the 2010 Gabonese outbreak. Infect. Dis. Rep. 2012, 4, e5. [Google Scholar] [CrossRef] [PubMed]
- Vazeille, M.; Moutailler, S.; Pages, F.; Jarjaval, F.; Failloux, A.B. Introduction of Aedes albopictus in Gabon: What consequences for dengue and chikungunya transmission? Trop. Med. Int. Health 2008, 13, 1176–1179. [Google Scholar] [CrossRef] [PubMed]
- Nkoghe, D.; Kassa, R.F.; Caron, M.; Grard, G.; Mombo, I.; Bikie, B.; Paupy, C.; Becquart, P.; Bisvigou, U.; Leroy, E.M. Clinical Forms of Chikungunya in Gabon, 2010. PLoS Negl. Trop. Dis. 2012, 6, e1517. [Google Scholar] [CrossRef] [PubMed]
- Gabor, J.J.; Schwarz, N.G.; Esen, M.; Kremsner, P.G.; Grobusch, M.P. Dengue and chikungunya seroprevalence in Gabonese infants prior to major outbreaks in 2007 and 2010: A sero-epidemiological study. Travel Med. Infect. Dis. 2016, 14, 26–31. [Google Scholar] [CrossRef] [PubMed]
- Peyrefitte, C.N.; Bessaud, M.; Pastorino, B.A.M.; Gravier, P.; Plumet, S.; Merle, O.L.; Moltini, I.; Coppin, E.; Tock, F.; Daries, W.; et al. Circulation of Chikungunya Virus in Gabon, 2006–2007. J. Med. Virol. 2008, 80, 430–433. [Google Scholar] [CrossRef]
- Pages, F.; Peyrefitte, C.N.; Mve, M.T.; Jarjaval, F.; Brisse, S.; Iteman, I.; Gravier, P.; Nkoghe, D.; Grandadam, M. Aedes albopictus Mosquito: The Main Vector of the 2007 Chikungunya Outbreak in Gabon. PLoS ONE 2009, 4, e4691. [Google Scholar] [CrossRef]
- Paupy, C.; Kassa Kassa, F.; Caron, M.; Nkoghé, D.; Leroy, E.M. A Chikungunya Outbreak Associated with the Vector Aedes albopictus in Remote Villages of Gabon. Vector-Borne Zoonotic Dis. 2012, 12, 167–169. [Google Scholar] [CrossRef] [PubMed]
- Dickson, L.B.; Sarah, H.; Merkling, S.H.; Gautier, M.; Ghozlane, A.; Jiolle, D.; Paupy, C.; Ayala, D.; Moltini-Conclois, I.; Fontaine, A.; et al. Exomewide association study reveals largely distinct gene sets underlying specific resistance to dengue virus types 1 and 3 in Aedes aegypti. PLoS Genet. 2020, 16, e1008794. [Google Scholar] [CrossRef] [PubMed]
- Caron, M.; Grard, G.; Paupy, C.; Mombo, I.M.; Bikie, B.N.B.; Kassa Kassa, F.R.; Nkoghe, D.; Leroy, E.M. First Evidence of Simultaneous Circulation of Three Different Dengue Virus Serotypes in Africa. PLoS ONE 2013, 8, e78030. [Google Scholar] [CrossRef] [PubMed]
- Abe, H.; Ushijima, Y.; Massinga, L.M.; Bikangui, R.; Nguema-Ondo, G.; Mpingabo, P.; Zadeh, R.V.; Pemba, C.M.; Kurosaki, Y.; Igasaki, Y. Re-emergence of dengue virus serotype 3 infections in Gabon in 2016–2017, and evidence for the risk of repeated dengue virus infections. Int. J. Infect. Dis. 2020, 91, 129–136. [Google Scholar] [CrossRef]
- Lim, J.K.; Fernandes, J.F.; Yoon, I.-K.; Lee, I.J.; Mba, R.O.; Lee, K.S.; Namkung, S.; Yang, J.S.; Bae, S.H.; Lim, S.-K.; et al. Epidemiology of dengue fever in Gabon: Results from a health facility-based fever surveillance in Lambaréné and its surroundings. PLoS Negl. Trop. Dis. 2021, 15, e0008861. [Google Scholar] [CrossRef]
- Xia, S.; Cosme, L.V.; Lutomiah, J.; Sang, R.; Ngangue, M.F.; Rahola, N.; Ayala, D.; Powell, J.R. Genetic structure of the mosquito Aedes aegypti in local forest and domestic habitats in Gabon and Kenya. Parasites Vectors 2020, 13, 417. [Google Scholar] [CrossRef]
- Pamba, R.; Koumba, A.A.; Zinga-Koumba, C.R.; Sevidzem, S.L.; Mbouloungou, A.; Yacka, L.L.; Djogbenou, L.S.; Mavoungou, J.F.; M’Batchi, B. Typology of Breeding Sites and Species Diversity of Culicids (Diptera: Culicidae) in Akanda and its Environs (North West, Gabon). Eur. J. Biol. Biotechnol. 2020, 1, 5. [Google Scholar]
- Obame-Nkoghe, J.; Makanga, B.K.; Zongo, S.B.; Koumba, A.A.; Komba, P.; Longo-Pendy, N.-M.; Mounioko, F.; Akone-Ella, R.; Nkoghe-Nkoge, L.-C.; Ngangue-Salamba, M.-F.; et al. Urban Green Spaces and Vector-Borne Disease Risk in Africa: Case of the Sibang Forested Park in Libreville (Gabon, CenTral Africa). Int. J. Environ. Res. Public Health 2023, 20, 5774. [Google Scholar] [CrossRef]
- Obame-Nkoghe, J.; Roiz, D.; Ngangue, M.F.; Costantini, C.; Rahola, N.; Jiolle, D.; Lehmann, D.; Makaga, L.; Ayala, D.; Kengne, P.; et al. Towards the of wild and rural forested areas in Gabon (Central Africa) by the Asian tiger mosquito: Potential risk from the One Health perspective. PLoS Negl. Trop. Dis. 2023, 17, e0011501. [Google Scholar] [CrossRef]
- Pourrut, X.; NKogué, D.; Souris, M.; Paupy, C.; Pawweska, J.; Padilla, C.; Moussavou, G.; Leroy, E.M. Rift Valley Fever Virus Seroprevalence in Human Rural Populations of Gabon. PLoS Negl. Trop. Dis. 2010, 4, e763. [Google Scholar] [CrossRef]
- Arya, S.C.; Agarwa, N. Apropos “Outbreak of Chikungunya in the Republic of Congo and the global picture”. J. Infect. Dev. Ctries. 2011, 5, 609–610. [Google Scholar] [CrossRef] [PubMed]
- Fritza, M.; Taty, R.T.; Portella, C.; Guimbi, C.; Mankou, M.; Leroy, E.M.; Becquart, P. Re-emergence of chikungunya in the Republic of the Congo in 2019 associated with a possible vector-host switch. Int. J. Infect. Dis. 2019, 84, 99–101. [Google Scholar] [CrossRef] [PubMed]
- Moyen, N.; Thiberville, S.-D.; Pastorino, B.; Nougairede, A.; Thirion, L.; Mombouli, J.-V.; Dimi, Y.; Leparc-Goffart, I.; Capobianchi, M.R.; Lepfoundzou, A.D.; et al. First Reported Chikungunya Fever Outbreak in the Republic of Congo, 2011. PLoS ONE 2014, 9, e115938. [Google Scholar] [CrossRef] [PubMed]
- Kamgang, B.; Wilson-Bahun, T.A.; Yougang, A.P.; Lenga, A.; Wondji, C.S. Contrasting resistance patterns to type I and II pyrethroids in two major arbovirus vectors Aedes aegypti and Aedes albopictus in the Republic of the Congo, Central Africa. Infect. Dis. Poverty 2020, 9, 23. [Google Scholar] [CrossRef]
- Nurtop, E.; Moyen, N.; Dzia-Lepfoundzou, A.; Dimi, Y.; Ninove, L.; Drexler, J.F.; Gallian, P.; de Lamballerie, X.; Priet, S. A Report of Zika Virus Seroprevalence in Republic of the Congo. Vector-Borne Zoonotic Dis. 2020, 20, 40–42. [Google Scholar] [CrossRef]
- Bitsindou, P.; Bantsimba-Ndziona, M.J.; Lenga, A. Distribution actuelle et caractérisations bioécologiques d’Aedes aegypti et d’Aedes albopictus dans deux arrondissements de Brazzaville. Bull. Soc. Pathol. Exot. 2018, 111, 301–308. [Google Scholar] [CrossRef]
- Guzman, M.G.; Halstead, S.B.; Artsob, H.; Buchy, P.; Farrar, J.; Gubler, D.J.; Hunsperger, E.; Kroeger, A.; Margolis, H.S.; Martinez, E. Dengue: A continuing global threat. Nat. Rev. Microbiol. 2010, 8, S7–S16. [Google Scholar] [CrossRef]
- Bhatt, S.; Gething, P.W.; Brady, O.J.; Messina, J.P.; Farlow, A.W.; Moyes, C.L.; Drake, J.M.; Brownstein, J.S.; Hoen, A.G.; Sankoh, O.; et al. The global distribution and burden of dengue. Nature 2013, 496, 504–507. [Google Scholar] [CrossRef]
- Sado, F.Y.; Tchetgna, H.S.; Kamgang, B.; Djonabaye, D.; Nakouné, E. Seroprevalence of Rift Valley fever virus in domestic ruminants of various origins in two markets of Yaoundé, Cameroon. PLoS Negl. Trop. Dis. 2022, 16, e0010683. [Google Scholar] [CrossRef]
- Ebogo-Belobo, J.T.; Sadeuh-Mba, S.A.; Mveng-Sanding, G.M.A.; Chavely, G.M.; Groschup, M.H.; Mbacham, W.F.; Njouom, R. Serological evidence of the circulation of the Rift Valley fever virus in sheep and goats slaughtered in Yaoundé, Cameroon. Vet. Med. Sci. 2022, 8, 2114–2118. [Google Scholar] [CrossRef]
- Liang, G.; Gao, X.; Gould, E.A. Factors responsible for the emergence of arboviruses; strategies, challenges and limitations for their control. Emerg. Microbes Infect. 2015, 4, e18. [Google Scholar] [CrossRef] [PubMed]
- Kraemer, M.U.G.; Faria, N.R.; Reiner, R.C., Jr.; Golding, N.; Nikolay, B.; Stasse, S.; Johansson, M.A.; Salje, H.; Faye, O.; Wint, G.R.W.; et al. Spread of yellow fever virus outbreak in Angola and the Democratic Republic of the Congo 2015–16: A modelling study. Lancet Infect. Dis. 2016, 3, 330–338. [Google Scholar] [CrossRef] [PubMed]
- Tajudeen, Y.A.; Oladipo, H.J.; Oladunjoye, I.O.; Yusuf, R.O.; Sodiq, H.; Omotosho, A.O.; Adesuyi, D.S.; Yusuff, S.I.; El-Sherbini, M.S. Emerging Arboviruses of Public Health Concern in Africa: Priorities for Future Research and Control Strategies. Challenges 2022, 13, 60. [Google Scholar] [CrossRef]
- Lim, J.K.; Ridde, V.; Agnandji, S.T.; Lell, B.; Yaro, S.; Yang, J.S.; Hoinard, D.; Weaver, S.C.; Vanhomwegen, J.; Salje, H.; et al. Seroepidemiological Reconstruction of Long-term Chikungunya Virus Circulation in Burkina Faso and Gabon. J. Infect. Dis. 2023, 227, 261–267. [Google Scholar] [CrossRef] [PubMed]
- Tchetgna, H.S.; Ouilibona, R.S.; Nkili-Meyong, A.A.; Caron, M.; Labouba, I.; Selekon, B.; Njouom, R.; Leroy, E.M.; Nakoune, E.; Berthet, N. Viral Exploration of Negative Acute Febrile Cases Observed during Chikungunya Outbreaks in Gabon. Intervirology 2019, 61, 174–184. [Google Scholar]
- Pourrut, X.; Nkoghé, D.; Gonzalez, J.P.; Leroy, E.M. No Evidence of Dengue Virus Circulation in Rural Gabon. Emerg. Infect. Dis. 2011, 17, 1568–1569. [Google Scholar] [CrossRef] [PubMed]
- Simo, F.B.N.; Bigna, J.J.; Kenmoe, S.; Ndangang, M.S.; Temfack, E.; Moundipa, P.F.; Demanou, M. Dengue virus infection in people residing in Africa: A systematic review and meta-analysis of prevalence studies. Sci. Rep. 2019, 9, 13626. [Google Scholar] [CrossRef]
- Ido, E.; Ahuka, S.; Karhemere, S.; Shibata, K.; Kameoka, M.; Muyembe, J.J. Infection du virus de la dengue survenue lors d’une épidémie du virus chikungunya en République démocratique du Congo. Ann. Afr. Med. 2017, 10, 3. [Google Scholar]
- Selhorst, P.; Makiala-Mandanda, S.; De Smet, B.; Mariën, J.; Anthony, C.; Binene-Mbuka, G.; De Weggheleire, A.; Ilombe, G.; Kinganda-Lusamaki, E.; Pukuta-Simbu, E.; et al. Molecular characterization of chikungunya virus during the 2019 outbreak in the Democratic Republic of the Congo. Emerg. Microbes Infect. 2020, 9, 1912–1918. [Google Scholar] [CrossRef]
- Biboussi, B. Epidémie de Fièvre Virale Chikungunya au Congo; N° 098 5 Juillet 2011; WHO: Geneva, Switzerland, 2011. [Google Scholar]
- Sebastiãoa, C.S.; Neto, Z.; Jandondo, D.; Mirandela, M.; Morais, J.; Britoa, M. Dengue virus among HIV-infected pregnant women attending antenatal care in Luanda, Angola: An emerging public health Concern. Sci. Afr. 2022, 17, e01356. [Google Scholar] [CrossRef]
- Takaya, S.; Kutsuna, S.; Nakayama, E.; Taniguchi, S.; Tajima, S.; Katanami, Y.; Yamamoto, K.; Takeshita, N.; Hayakawa, K.; Kato, Y.; et al. Chikungunya Fever in Traveler from Angola to Japan, 2016. Emerg. Infect. Dis. 2017, 23, 156. [Google Scholar] [CrossRef] [PubMed]
- Vasconcelos, P.F.C.; Monath, T.P. Yellow fever remains a potential threat to public health. Vector-Borne Zoonotic Dis. 2016, 16, 566–567. [Google Scholar] [CrossRef]
- Nakouné, E.; Selekon, B.; Morvan, J. Summary: Microbiological surveillance: Viral haemorrhagic fevers in the Central African Republic. Santé Publique 2000, 8, 2035. [Google Scholar]
- Wilson-Bahun, T.A.; Kamgang, B.; Lenga, A.; Wondji, C.S. Larval ecology and infestation indices of two major arbovirus vectors, Aedes aegypti and Aedes albopictus (Diptera: Culicidea), in Brazzille, the capital city of the Republic of the Congo. Parasits Vectors 2020, 13, 492. [Google Scholar] [CrossRef]
- Vianney, B.J.M.; Diakaridia, F.; Yahaya, S.; Koné, A.B.; Lambert, K.K.; Sevidzem, S.L.; Acapovi-Yao, G.L. Molecular detection of arboviruses in culicidae in some sites of Côte d’Ivoire. Int. J. Biol. 2021, 19, 125–138. [Google Scholar]
- Mayi, M.P.A.; Bamou, R.; Djiappi-Tchamen, B.; Fontaine, A.; Jeffries, C.L.; Walker, T.; Antonio-Nkondjio, C.; Cornel, A.J.; Tchuinkam, T. Habitat and Seasonality Affect Mosquito Community Composition in the West Region of Cameroon. Insects 2020, 11, 312. [Google Scholar] [CrossRef]
- Souza-Neto, J.A.; Powell, J.R.; Bonizzoni, M. Aedes aegypti vector competence studies: A review. Infect. Genet. Evol. 2019, 67, 191–209. [Google Scholar] [CrossRef]
- Diallo, M.; Laganier, R.; Nangouma, A. First record of Ae. albopictus (Skuse 1894), in Central African Republic. Trop. Med. Int. Health 2010, 15, 1185–1189. [Google Scholar] [CrossRef]
- Kamgang, B.; Ngoagouni, C.; Manirakiza, A.; Nakoune, E.; Paupy, C.; Kazanji, M. Temporal Patterns of Abundance of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) and Mitochondrial DNA Analysis of Ae. albopictus in the Central African Republic. PLoS Negl. Trop. Dis. 2013, 7, e2590. [Google Scholar] [CrossRef]
- Paupy, C.; Delatte, H.; Bagny, L.; Corbel, V.; Fontenille, D. Aedes albopictus, an arbovirus vector: From the darkness to the light. Microbes Infect. 2009, 11, 1177–1185. [Google Scholar] [CrossRef]
- Kamgang, B.; Vazeille, M.; Tedjou, A.N.; Wilson-Bahun, T.A.; Yougang, A.P.; Mousson, L.; Wondji, C.S.; Failloux, A.B. Risk of dengue in Central Africa: Vector competence studies with Aedes aegypti and Aedes albopictus (Diptera: Culicidae) populations and dengue 2 virus. PLoS Negl. Trop. Dis. 2019, 13, e0007985. [Google Scholar] [CrossRef] [PubMed]
- Simard, F.; Nchoutpouen, E.; Toto, J.C.; Fontenille, D. Geographic Distribution and Breeding Site Preference of Aedes albopictus and Aedes aegypti (Diptera: Culicidae) in Cameroon, Central Africa. Entomol. Soc. Am. 2005, 42, 726–731. [Google Scholar] [CrossRef]
- Saotoing, P.; Tchuenguem, F.N.F.; Nlôga, A.M.N. Entomological Survey on Culicidae fauna in the City of Maroua, Far North Region Cameroon. Int. J. Innov. 2014, 9, 438–448. [Google Scholar]
- Akono-Ntonga, P.; Peka-Nsangou, M.F.; Kekeunou, S.; Kojom-Fozie-Kamga, R.; Tonga, C.; Ngo-Hondt, E.; Mbida, J.A. Diversité et agressivité de la culicidofaune dans la ville de Douala, Cameroun. Faun. Entomol. 2020, 73, 26–35. [Google Scholar]
- Bamou, R.; Mayi, M.P.A.; Djiappi-Tchamen, B.; Nana-Ndjangwo, S.M.; Nchoutpouen, E.; Cornel, A.J.; Awono-Ambene, P.; Parola, P.; Tchuinkam, T.; Antonio-Nkondjio, C. An update on the mosquito fauna and mosquito-borne diseases distribution in Cameroon. Parasites Vectors 2021, 14, 527. [Google Scholar] [CrossRef]
- Talipouo, A.; Akono, P.N.; Tagne, D.; Mbida, A.M.; Etang, J.; Fobasso, R.T.; Ekoko, W.; Binyang, J.; Dongmo, A. Comparative study of Culicidae biodiversity of Manoka island and Youpwe mainland area, Littoral Cameroon. Int. J. Biosci. 2017, 10, 9–18. [Google Scholar]
- Ntoumba, A.A.; Foko, L.P.K.; Ekoko, W.E.; Ndongo, J.M.; Bunda, G.W.; Meva, F.E.; Lehman, L.G. Entomological characteristics of mosquitoes breeding sites in two areas of the town of Douala, Cameroon. Int. J. Trop. Insect Sci. 2020, 41, 1313–1323. [Google Scholar] [CrossRef]
- Paupy, C.; Brengues, C.; Kamgang, B.; Herve, J.P.; Fontenille, D.; Simard, F. Gene Flow between Domestic and Sylvan Populations of Aedes aegypti (Diptera: Culicidae) in North Cameroon. J. Med. Entomol. 2008, 45, 391–400. [Google Scholar] [CrossRef]
- Fontenille, D.; Toto, J.C. Aedes (Stegomyia) albopictus (Skuse), vecteur potentiel du virus de la dengue, a envahi les villes du sud du Cameroun; Maladies à transmission vectorielle en milieu urbain. Emerg. Infect. Dis. 2001, 7, 1066–1067. [Google Scholar] [CrossRef]
- Kamgang, B.; Brengues, C.; Fontenille, D.; Njiokou, F.; Simard, F.; Paupy, C. Genetic Structure of the Tiger Mosquito, Aedes albopictus, in Cameroon (Central Africa). PLoS ONE 2011, 6, e20257. [Google Scholar] [CrossRef]
- Bakwo-Fils, E.M.; Ntonga-Akono, P.; Belong, P.; Messi, J. Impact des aménagements piscicoles sur le pullulement culicidien à Yaoundé, Cameroun. Faun. Entomol. 2009, 62, 109–114. [Google Scholar]
- Tedjou, A.N.; Kamgang, B.; Yougang, A.P.; Njiokou, F.; Wondji, C.S. Update on the geographical distribution and prevalence of Aedes aegypti and Aedes albopictus (Diptera: Culicidae), two major arbovirus vectors in Cameroon. PLoS Negl. Trop. Dis. 2019, 13, e0007137. [Google Scholar] [CrossRef] [PubMed]
- Kamgang, B.; Vazeille, M.; Youganga, A.P.; Tedjoua, A.N.; Wilson-Bahun, T.A.; Mousson, L.; Wondji, C.S.; Failloux, A.B. Potential of Aedes albopictus and Aedes aegypti (Diptera: Culicidae) to transmit yellow fever virus in urban areas in Central Africa. Emerg. Microbes Infect. 2019, 8, 1636–1641. [Google Scholar] [CrossRef] [PubMed]
- Mbida, M.A.; Etang, J.; Ntonga, A.P.; Talipouo, A.; Awono-Ambene, P.; Oke-Agbo, F.; Eboumbou, C.; Akogbéto, M.; Osse, R.; Lehman, G.; et al. Preliminary investigation on aggressive culicidae fauna and malaria transmission in two wetlands of the Wouri river estuary, Littoral-Cameroon. J. Entomol. Zool. Stud. 2016, 4, 105–110. [Google Scholar]
- Kamgang, B.; Nchoutpouen, E.; Simard, F.; Paupy, C. Notes on the blood-feeding behavior of Aedes albopictus (Diptera: Culicidae) in Cameroon. Parasites Vectors 2012, 5, 57. [Google Scholar] [CrossRef]
- Krueger, A.; Hagen, R.M. First record of Aedes albopictus in Gabon, Central Africa. Trop. Med. Int. Health 2007, 12, 1105–1107. [Google Scholar] [CrossRef]
- Xia, S.; Dweck, H.K.M.; Lutomiah, J.; Sang, R.; McBride, C.S.; Rose, N.H.; Ayala, D.; Powell, J.R. Larval sites of the mosquito Aedes aegypti formosus in forest and domestic habitats in Africa and the potential association with oviposition evolution. Ecol. Evol. 2021, 11, 16327–16343. [Google Scholar] [CrossRef]
- Kamgang, B.; Wilson-Bahun, T.A.; Irving, H.; Kusimo, M.O.; Lenga, A.; Wondji, C.S. Geographical distribution of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) and genetic diversity of invading population of Ae. albopictus in the Republic of the Congo. Wellcome Open Res. 2018, 3, 79. [Google Scholar] [CrossRef]
- Mombouli, J.V.; Bitsindou, P.; Elion, D.O.A.; Grolla, A.; Feldmann, H.; Niama, F.R.; Parra, H.-J.; Vincent, J.; Munster, V.J. Chikungunya Virus Infection, Brazzaville, Republic of Congo, 2011. Emerg. Infect. Dis. 2018, 19, 1542. [Google Scholar] [CrossRef]
- Yougang, A.P.; Kamgang, B.; Wilson Bahun, T.A.; Tedjou, A.N.; Nguiffo-Nguete, D.; Njiokou, F.; Wondji, C.S. First detection of F1534C Knockdown resistance mutation in Aedes aegypti (Diptera: Culicidae) from Cameroun. Infect. Dis. Poverty 2020, 9, 51–62. [Google Scholar] [CrossRef]
- Djiappi-Tchamen, B.; Nana-Ndjangwo, M.S.; Mavridis, K.; Talipouo, A.; Nchoutpouen, E.; Makoudjou, I.; Bamou, R.; Mayi, A.M.P.; Awono-Ambene, P.; Tchuinkam, T.; et al. Analyses of Insecticide Resistance Genes in Aedes aegypti and Aedes albopictus Mosquito Populations from Cameroon. Genes 2021, 12, 828. [Google Scholar] [CrossRef] [PubMed]
- Vazeille, M.; Failloux, A.N.; Mousson, L.; Elissa, N.; Roudhain, F. Receptivité orale d’Aedes aegypti formosus de Franceville (Gabon, Afrique centrale) pour le virus de la dengue de typtes 2. Bull. Soc. Pathol. Exot. 1999, 92, 341–342. [Google Scholar]
- Jiolle, D.; Moltini-Conclois, I.; Obame-Nkoghe, J.; Yangari, P.; Porciani, A.; Scheid, B.; Kengne, P.; Ayala, D.; Failloux, A.B.; Paupy, C. Experimental infections with Zika virus strains reveal high vector competence of Aedes albopictus and Aedes aegypti populations from Gabon (Central Africa) for the African virus lineage Emerging. Microbes Infect. 2021, 10, 1245–1253. [Google Scholar] [CrossRef] [PubMed]
- Coffinet, T.; Mourou, J.R.; Pradines, B.; Toto, J.C.; Jarjaval, F.; Amalvict, R.; Kombila, M.; Carnevale, P.; Pages, F. First record of Aedes albopictus in Gabon. J. Am. Mosq. Control Assoc. 2007, 23, 471–472. [Google Scholar] [CrossRef]
- Bobanga, T.; Moyo, M.; Vulu, F.; Irish, S.R. First report of Aedes albopictus (Diptera: Culicidae) in the Democratic Republic of Congo. Afr. Entomol. 2018, 26, 234–236. [Google Scholar] [CrossRef]
- Kamgang, B.; Marcombe, S.; Chandre, F.; Nchoutpouen, E.; Nwane, P.; Etang, J.; Corbel, V.; Paupy, C. Insecticide susceptibility of Aedes aegypti and Aedes albopictus in Central Africa. Parasit Vectors 2011, 4, 79. [Google Scholar] [CrossRef]
- Bamou, R.; Diarra, A.; Mayi, M.P.A.; Djiappi-Tchamen, B.; Antonio-Nkondjio, C.; Parola, P. Wolbachia Detection in Field-Collected Mosquitoes from Cameroon. Insects 2021, 12, 1133. [Google Scholar] [CrossRef]
- Osuna, A.M.; Gidley, A.; Mayi, M.P.A.; Bamou, R.; Dhokiya, V.; Antonio-Nkondjio, C.; Jeffries, C.L.; Walker, T. Diverse novel Wolbachia bacteria strains and widespread co-infections with Asaia in Culicine mosquitoes from ecologically diverse regions of Cameroom. Wellcome Open Res. 2023, 8, 267. [Google Scholar]
- Grard, G.; Moureau, G.; Charrel, R.N.; Holmes, E.C.; Gould, E.A.; de Lamballerie, X. Genomics and evolution of Aedes-borne flaviviruses. J. Gen. Virol. 2010, 91, 87–94. [Google Scholar] [CrossRef]
- Canelas, T.; Thomsen, E.; Kamgang, B.; Kelly-Hope, L.A. Demographic and environmental factors associated with the distribution of Aedes albopictus in Cameroon. Med. Vet. Entomol. 2023, 37, 143–151. [Google Scholar] [CrossRef]
- Agbodzi, B.; Sado, F.B.Y.; Simo, F.B.N.; Kumordjie, S.; Yeboah, C.; Mosore, M.-T.; Bentil, R.E.; Prieto, K.; Colston, S.M.; Attram, N.; et al. Chikungunya viruses containing the A226V mutation detected retrospectively in Cameroon form a new geographical subclade. Int. J. Infect. Dis. 2021, 113, 65–73. [Google Scholar] [CrossRef] [PubMed]
- Socolovschi, C.; Pagés, F.; Raoult, D. Rickettsia felis in Aedes albopictus Mosquitoes, Libreville, Gabon. Emerg. Infect. Dis. 2012, 18, 1687–1688. [Google Scholar] [CrossRef] [PubMed]
- Makanga, B.K.; Koumba, A.A.; Makouloutou, P.; Mougoubi, J.W.; Koumba, C.R.Z.; Mavoungou, J.F. Diversité de la culicidofaune et risques potentiels de maladies dans le Parc National de Loango au Gabon. Int. J. Innov. Appl. Stud. 2022, 37, 368–377. [Google Scholar]
- Obame-Nkoghe, J.; Rahola, N.; Ayala, D.; Yangari, P.; Jiolle, D.; Allene, X.; Bourgarel, M.; Maganga, G.D.; Berthet, N.; Leroy, E.M.; et al. Exploring the diversity of bloodsucking Diptera in caves of Central Africa. Sci. Rep. 2017, 7, 250. [Google Scholar] [CrossRef] [PubMed]
- Irish, S.R.; Kyalo, D.; Snow, R.W.; Coetzee, M. Updated list of Anopheles species (Diptera: Culicidae) by country in the Afrotropical Region and associated islands. Zootaxa 2020, 4747, 401–449. [Google Scholar] [CrossRef] [PubMed]
- Kerah-Hinzoumbé, C.; Péka, M.; Nkondjio, C.A.; Donan-Gouni, I.; Awono-Ambene, P.; Samè-Ekobo, A.; Simard, F. Malaria vectors and transmission dynamics in Goulmoun, a rural city in south-western Chad. BMC Infect. Dis. 2009, 9, 71. [Google Scholar] [CrossRef] [PubMed]
- Tavares, W.; Morais, J.; Martins, J.F.; Scalsky, R.J.; Stabler, T.C.; Medeiros, M.M.; Fortes, F.J.; Arez, A.P.; Silva, J.C. Malaria in Angola: Recent progress, challenges and future opportunities using parasite demography studies. Malar. J. 2022, 21, 396. [Google Scholar] [CrossRef] [PubMed]
- Trape, J.F.; Zoulani, A. Malaria and urbanization in Central Africa: The example of Brazzaville: Part II: Results of entomological surveys and epidemiological analysis. Trans. R. Soc. Trop. Med. Hyg. 1987, 81, 10–18. [Google Scholar] [CrossRef] [PubMed]
- Ridl, F.C.; Bass, C.; Torrez, M.; Govender, D.; Ramdeen, V.; Yellot, L.; Edu, A.E.; Schwabe, C.; Mohloai, P.; Maharaj, R.; et al. A pre-intervention study of malaria vector abundance in Rio Muni, Equatorial Guinea: Their role in malaria transmission and the incidence of insecticide resistance alleles. Malar. J. 2008, 7, 194. [Google Scholar] [CrossRef] [PubMed]
- Makanga, B.; Yangari, P.; Rahola, N.; Rougeron, V.; Elguero, E.; Boudenga, L.; Moukodoum, N.D.; Okouga, A.P.; Arnathau, C.; Durand, P.; et al. Malaria transmission and potential for Ape to human transfers in Africa. Proc. Natl. Acad. Sci. USA 2016, 113, 5329–5334. [Google Scholar] [CrossRef]
- Koumba, A.A.; Koumba, C.R.Z.; Nguema, R.M.; Zahouli, B.J.Z.; Ovono, A.M.; Souza, A.; Ketoh, G.K.; Djogbenou, L.S.; M’batchi, B.; Mavoungou, J.F. Preliminary evaluation of the insecticide susceptibility in the Culicid Fauna, particularity Malaria plasmodium and Arbovirus vectors in the region of Mouila, South-West GABON. Indian J. Med. Res. Pharm. Sci. 2018, 5, 105–117. [Google Scholar]
- Mayi, M.P.A.; Foncha, D.F.; Kowo, C.; Tchuinkam, T.; Brisco, K.; Anong, D.N.; Ravinder, S.; Cornel, A.J. Impact of deforestation on the abundance, diversity, and richness of Culex mosquitoes in a southwest Cameroon tropical rainforest. J. Vector Ecol. 2019, 44, 271–281. [Google Scholar] [CrossRef] [PubMed]
- Djoufounna, J.; Mayi, M.P.A.; Bamou, R.; Ningahi, L.G.; Magatsing, F.O.; Djiappi-Tchamen, B.; Djamouko-Djonkam, L.; Nkondjio, C.A.; Tchuinkam, T. Larval habitats characterization and population dynamics of Culex mosquitoes in two localities of the Menoua Division, Dschang and Santchou, West Cameroon. J. Basic Appl. Zool. 2022, 83, 30. [Google Scholar] [CrossRef]
- Cordelier, R.; Geoffroy, B. Contribution à l’étude des Culicides de la République Centrafricaine Rythmes d’activités en Secteur Préforestier. Cah. ORSTOM Ser. Entomol. Med. Parasitol. 1974, 12, 19–48. [Google Scholar]
- Bouree, P.; Ensaf, A. Aedes albopictus: A Multifunctional Mosquito; Elsevier: Amsterdam, The Netherlands, 2015; p. 519. [Google Scholar]
- Braack, L.; d’Almeida, A.P.G.; Cornel, A.J.; Swanepoel, R.; de Jage, C. Mosquito-borne arboviruses of African origin: Review of key viruses and vectors. Parasites Vectors 2018, 11, 29. [Google Scholar] [CrossRef]
- Gérardin, P. Fièvre à virus Chikungunya: Progrès en Pédiatrie, France. In Pédiatrie Tropicale et des Voyages; Chapitre 25; EDUCA Books, 2012; pp. 285–292. Available online: https://www.researchgate.net/publication/273119120_Fievre_a_virus_Chikungunya_In_P_Imbert_P_Minodier_ed_Pediatrie_tropicale_et_des_voyages_Progres_en_Pediatrie_P_Cochat_ed_Doin_Wolters_Kluwer_France_2012_pp_285-292 (accessed on 10 October 2023).
- Turell, M.; Linthicum, K.J.; Patrican, L.A.; Davies, F.G.; Kairo, A.; Charles, L.; Bailey, C.L. Vector Competence of Selected African Mosquito (Diptera: Culicidae) Species for Rift Valley Fever Virus. Vect. Path. Host Int. Trans. 2008, 45, 102–108. [Google Scholar] [CrossRef]
- Armand, C.C. Etude Bibliographique des Zoonoses en Côte d’ivoire. Ph.D. Thesis, Ecole Nationale Vétérinaire de Toulouse, Toulouse, France, 2001; 160p. [Google Scholar]
- Cornet, M.; Robin, Y.; Château, R.; Heme, G.; Adam, C.; Valade, M.; Le Gonidec, G.; Jan, C.; Renaudet, J.; Dieng, P.L.; et al. Isolements d’arbovirus au Sénégal Oriental h partir de moustiques (1972–4977) et notes sur l’épidémiologie des virus transmis par les Aedes, en particulier du virus amaril. Cah. ORSTOM Ser. Entomol. Med. Parasitol. 1979, 17, 149–163. [Google Scholar]
- Thiemann, T.C.; Lemenager, D.A.; Kluh, S.; Carrol, B.D.; Lothrop, H.D.; Keisen, W.K. Spatial Variation in Host Feeding Patterns of Culex tarsalis and the Culex pipiens complex (Diptera: Culicidae) in California. J. Med. Entomol. 2012, 49, 903–916. [Google Scholar] [CrossRef]
- Cordelier, R.; Geoffroy, B. Observqtions sur les vecteurs potentiels de la fievre jaune en Républiaque Centrafricaine. Cah. ORSTOM Ser. Entomol. Med. Parasitol. 1972, 10, 127–144. [Google Scholar]
- Gratz, N.G. Critical review of the vector status of Aedes albopictus. Med. Vet. Entomol. 2004, 18, 215–227. [Google Scholar] [CrossRef]
- Wong, P.-S.J.; Li, M.-Z.I.; Chong, C.-S.; Ng, L.-C.; Tan, C.-H. Aedes (Stegomyia) albopictus (Skuse): A potential vector of Zika virus in Singapore. PLoS Negl. Trop. Dis. 2013, 7, e2348. [Google Scholar] [CrossRef] [PubMed]
- WHO. Vector-Borne Diseases. 2020. Available online: www.who.int (accessed on 30 May 2023).
- Fros, J.J.; Miesen, P.; Vogels, C.B.; Gaibani, P.; Sambri, V.; Martina, B.E.; Koenraadt, C.J.; Rij, R.P.V.; Vlak, J.M.; Takken, W.; et al. Comparative Usutu and West Nile virus transmission potential by local Culex pipiens mosquitoes in north-western Europe. One Health 2015, 1, 31–36. [Google Scholar] [CrossRef] [PubMed]
- Ochieng, C.; Lutomiah, J.; Makio, A.; Koka, H.; Chepkorir, E.; Yalwala, S.; Mutisya, J.; Musila, L.; Khamadi, S.; Richardson, J.; et al. Mosquito-borne arbovirus surveillance at selected sites in diverse ecological zones of Kenya; 2007–2012. Virol. J. 2013, 10, 140. [Google Scholar] [CrossRef] [PubMed]
- Pinto, M.R.; Filipe, A.R. Arbovirus studies in Luanda, Angola. Virological and serological studies during a yellow fever epidemic. Bull. Org. Mond. Sant. 1973, 49, 31–35. [Google Scholar]
- Huang, Y.-M. The subgenus Stegomyia of Aedes in the Afrotropical Region with keys to the species (Diptera: Culicidae). Zootaxa 2004, 700, 27. [Google Scholar] [CrossRef]
- Adam Digoutte, D. Pasteur Institute and IRD CRORA. 2005. Available online: http://www.pasteur.fr/recherche/banques/CRORAdatabase (accessed on 5 October 2023).
- Germain, M.; Robin, Y.; Geoffrey, B.; Cornet, M.; Vauchez, M.F. Isolements du virus de la fièvre jaune à partir d’Aedes du groupe A. africanus (Theobald) en République Centrafricaine. Importance des savanes humides et semi-humides en tant que zone d’émergence du virus amaril. Cah. ORSTOM Ser. Entomol. Med. Parasitol. 1976, 14, 125–139. [Google Scholar]
- Kokernot, R.H.; Paterso, H.E.; De Meillon, B. Studies on the transmission of wesselsbron virus by Aedes (Oclherotatus) Caballus (Theo). Med. J. 1958, 32, 546–548. [Google Scholar]
- White, G.B. Notes on a Catalogue of Culicidae of the Ethiopian Region. Mosq. Syst. 1975, 7, 303–344. [Google Scholar]
- Diallo, M. Dynamique comparée des populations de Culicidae à Kédougou (zone soudano-guinéenne) et à Barkédji (zone de savane sahélienne): Conséquences dans la transmission des arbovirus. Univ. Cheikh Anta Diop Dakar Fac. Sci. Tech. 1995, 96. [Google Scholar]
- Blanchard, R. Les Moustiques: Histoire Naturelle et Médicale; Rudeval: Paris, France, 1905. [Google Scholar]
- Brottes, H.; Rickenbach, A.; Brls, P.; Salaun, J.J.; Feraa, L. Les arbovirus au Cameroun, Isolements à partir de moustiques. Bull. World Health Organ. 1966, 35, 811–825. [Google Scholar]
- Ernest Gould, E.; Pettersson, J.; Higgse, S.; Charrela, R.; de Lamballerie, X. Emerging arboviruses: Why today? One Health 2017, 4, 1–13. [Google Scholar] [CrossRef] [PubMed]
- Salaun, J.J.; Rickenbach, A.; Brts, P.; Brottes, H.; Germain, M.; Eouzan, J.-P.; Ferrara, L. Les arbovirus isolés à partir de moustiques au Cameroun. Bull. World Health Organ. 1969, 41, 233–241. [Google Scholar] [PubMed]
- Ndiaye, E.H.; Fall, G.; Gaye, A.; Bob, N.S.; Talla, C.; Diagne, C.T.; Diallo, D.; Ba, Y.; Dia, I.; Kohl, A.; et al. Vector competence of Aedes vexans (Meigen), Culex poicilipes (Theobald) and Cx. quinquefasciatus Say from Senegal for West and East African lineages of Rift Valley fever virus. Parasites Vectors 2016, 9, 94. [Google Scholar] [CrossRef] [PubMed]
- Chippaux, A. Généralités sur arbovirus et arboviroses. Méd. Mal. Infect. 2003, 33, 377–384. [Google Scholar] [CrossRef]
- Morin-Rivat, J.; Fayolle, A.; Favier, C.; Bremond, L.; Gourlet-Fleury, S.; Bayol, N.; Lejeune, P.; Beeckman, H.; Doucet, J.-L. Present-day central African forest is a legacy of the 19th century human history. eLife 2017, 6, e20343. [Google Scholar] [CrossRef]
- Casal, J. Comparative Virology, Arboviruses: Incorporation in a General System of Virus Classification; Academic Press: Cambridge, MA, USA, 1979; pp. 307–333. [Google Scholar]
- WHO. Surveillance and Control of Arboviral Diseases in the WHO African Region World Health Organization on Behalf of the UNICEF/UNDP/World Bank/WHO; Special Programme for Research and Training in Tropical Diseases; WHO: Geneva, Switzerland, 2022; p. 148. [Google Scholar]
- Bourgarel, M.; Wauquier, N.; Gonzalez, J.P. Emerging viral threats in Gabon: Health capacities and response to the risk of emerging zoonotic diseases in Central Africa. Emerg. Health Threats J. 2010, 3, e7. [Google Scholar] [CrossRef]
- Madewell, Z.J. Arboviruses and Their Vectors. South. Med. J. 2020, 113, 520–523. [Google Scholar] [CrossRef]
- Fokam, E.B.; Levai, L.D.; Guzman, H.; Amelia, P.A.; Titanji, V.P.K.; Tesh, R.B.; Weaver, S.C. Silent circulation of arboviruses in Cameroon. East. Afr. Med. J. 2010, 87, 262. [Google Scholar] [CrossRef]
- Ushijima, Y.; Abe, H.; Ngema-Ondo, G.; Bikangui, R.; Massinga, L.M.; Zadeh, V.R.; Essimengane, J.G.E.; Mbouna, A.V.N.; Bache, E.B.; Agnandji, S.T.; et al. Surveillance of the major pathogenic arboviruses of public health concern in Gabon, Central Africa: Increased risk of West Nile virus and dengue virus infections. BMC Infect. Dis. 2021, 21, 265. [Google Scholar] [CrossRef]
- Neto, Z.; Martinez, P.A.; HillI, S.C.; Jandondo, D.; Theze, J.; Mirandela, M.; Aguiar, R.S.; Xavier, J.; Sebastião, C.D.S.; Candido, A.M.; et al. Molecular and genomic investigation of an urban outbreak of dengue virus serotype 2 in Angola, 2017–2019. PLoS Negl. Trop. Dis. 2022, 16, e0010255. [Google Scholar] [CrossRef]
- Demanou, M.; Antonio-Nkondjio, C.; Ngapana, E.; Rousset, D.; Paupy, C.; Manuguerra, J.-C.; Zeller, H. Chikungunya outbreak in a rural area of Western Cameroon in 2006: A retrospective serological and entomological survey. BMC Res. Notes 2010, 3, 128. [Google Scholar] [CrossRef] [PubMed]
- Ngoagouni, C.; Kamgang, B.; Manirakiza, A.; Nangouma, A.; Paupy, C.; Nakoune, E.; Kazanji, M. Entomological profile of yellow fever epidemics in the Central African Republic, 2006–2010. Parasites Vectors 2012, 5, 175. [Google Scholar] [CrossRef] [PubMed]
- Bessaud, M.; Peyrefitte, C.N.; Pastorino, B.A.M.; Gravier, P.; Tock, F.; Boete, F.; Tolou, H.J.; Grandadam, M. O’nyong-nyong virus, Chad. Emerg. Infect. Dis. 2006, 12, 1248–1250. [Google Scholar] [CrossRef]
- Ushijima, Y.; Abe, H.; Mbadinga, M.J.; Ondo, G.N.; Bikangui, R.; Agnandji, S.T.; Lell, B.; Yasuda, J. Re-emergence of dengue, chikungunya, and Zika viruses in 2021 after a 10-year gap in Gabon. IJID Reg. 2022, 5, 68–71. [Google Scholar] [CrossRef] [PubMed]
- Mbanzulu, K.M.; Wumba, R.; Mukendi, J.P.K.; Zanga, J.K.; Shija, F.; Bobanga, T.L.; Aloni, M.N.; Misinzo, G. Mosquito-borne viruses circulating in Kinshasa, Democratic Republic of the Congo. Int. J. Infect. Dis. 2017, 57, 32–37. [Google Scholar] [CrossRef] [PubMed]
- Collao, X.; Negredo, A.I.; Cano, J.; Tenorio, A.; de Ory, F.; Benito, A.; Masia, M.; Sánchez-Seco, M.-P. Different Lineages of Chikungunya Virus in Equatorial Guinea in 2002 and 2006. Am. J. Trop. Med. Hyg. 2010, 82, 505–507. [Google Scholar] [CrossRef]
- Vairo, F.; Coussoud-Mavoungou, M.P.A.; Ntoumi, F.; Castilletti, C.; Kitembo, L.; Haider, N.; Carletti, F.; Colavita, F.; Gruber, C.E.M.; Iannetta, M.; et al. Chikungunya Outbreak in the Republic of the Congo, 2019-Epidemiological, Virological and Entomological Findings of a South-North Multidisciplinary Taskforce Investigation. Viruses 2020, 12, 1020. [Google Scholar] [CrossRef]
- Massengo, N.R.B.; Tinto, B.; Simonin, Y. One Health Approach to Arbovirus Control in Africa: Interests, Challenges, and Difficulties. Microorganisms 2023, 11, 1496. [Google Scholar] [CrossRef]
- Zahouli, J.B.Z.; Koudou, B.G.; Müller, P.; Malone, D.; Tano, Y.; Utzinger, J. Urbanization is a main driver for the larval ecology of Aedes mosquitoes in arbovirus endemic settings in south-eastern Côte d’Ivoire. PLoS Negl. Trop. Dis. 2017, 11, e0005751. [Google Scholar] [CrossRef]
- Dongho, G.B.D.; Venturi, G.; Fortuna, C.; Paganotti, G.M.; Severini, C.L.; Episcopia, M.; Tsapi, A.T.; Benedetti, E.; Marsili, G.; Amendola, A.; et al. Dengue and Chikungunya virus circulation in Cameroon and Gabon: Molecular evidence among symptomatic individuals Access. Microbiology 2022, 2, 000340. [Google Scholar]
- Darriet, F. Des Moustiques et des Hommes. Chronique d’une Pullulation Annonée; coll. Didactiques; IRD: Marseille, France, 2014. [Google Scholar]
- Grard, G.; Caron, M.; Mombo, I.M.; Nkoghe, D.; Ondo, S.M.; Jiolle, D.; Fontenille, D.; Paupy, C.; Leroy, E.M. Zika Virus in Gabon (Central Africa)—2007: A New Threat from Aedes albopictus? PLoS Negl. Trop. Dis. 2014, 8, e2681. [Google Scholar] [CrossRef]
- Page, M.J.; Mckenzi, J.E.; Bossuyt, P.M.; Boutron, I.; Hoffmann, T.C.; Mulrow, C.D.; Shamseer, L.; Tetzlaff, J.M.; Akl, E.A.; Brennan, S.E.; et al. The PRISMA 2020 statement: An updated guideline for reporting systematic reviews. BJM 2021, 372, n71. [Google Scholar] [CrossRef] [PubMed]
- Moher, D.; Cook, D.J.; Eastwood, S.; Olkin, I.; Rennie, D.; Stroup, D.F.; QUOROM Group. Improving the quality of reports of meta-analyses of randomised controlled trials: The QUOROM statement. Br. J. Surg. 2000, 87, 1448–1454. [Google Scholar] [CrossRef] [PubMed]
- Mutebi, J.-P.; Barrett, A.D.T. The epidemiology of yellow fever in Africa. Microb. Infect. 2002, 4, 1459–1468. [Google Scholar] [CrossRef] [PubMed]
- Adam, A.; Jassoy, C. Epidemiology and Laboratory Diagnostics of Dengue, Yellow Fever, Zika, and Chikungunya Virus Infections in Africa. Pathogens 2021, 10, 1324. [Google Scholar] [CrossRef]
- Bakker, R.C.; Veenstra, J.; Dingemans-Dumas, A.M.; Wetsteyn, C.F.M.; Kager, P.A. Imported Dengue in the Netherlands. J. Travel Med. 1996, 3, 204–208. [Google Scholar] [CrossRef]
- Schwartz, E.; Meltzer, E.; Mendelson, M.; Tooke, A.; Steiner, F.; Gautret, P.; Friedrich-Jaenicke, B.; Libman, M.; Bin, H.; Wilder-Smith, A.; et al. Detection on four continents of dengue fever cases related to an ongoing outbreak in Luanda, Angola, March to May 2013. Euro Surveill. 2003, 18, 20488. [Google Scholar] [CrossRef]
- Sharp, T.M.; Moreira, R.; Soares, M.J.; da Costa, L.M.; Mann, J.; DeLorey, M.; Hunsperger, E.; Muñoz-Jordán, J.L.; Colón, C.; Margolis, H.S.; et al. Underrecognition of Dengue during 2013 Epidemic in Luanda, Angola. Emerg. Infect. Dis. 2015, 27, 1311–1316. [Google Scholar] [CrossRef]
- Santiago, G.A.; Vázquez, J.; Courtney, S.; Matías, K.Y.; Andersen, L.E.; Colón, C.; Butler, A.E.; Roulo, R.; Bowzard, J.; Villanueva, J.M.; et al. Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses. Nat. Commun. 2018, 9, 1391. [Google Scholar] [CrossRef]
- Parreira, R.; Centeno-Lima, S.; Lopes, A.; Portugal-Calisto, D.; Constantino, A.; Nina, J. Dengue virus serotype 4 and chikungunya virus coinfection in a traveller returning from Luanda, Angola. Euro Surveill. 2014, 19, 20730. [Google Scholar] [CrossRef]
- Filipe, A.R.; Pinto, M.R. Arbovirus studies in Luanda, Angola. Bull. World Health Organ. 1973, 49, 37–40. [Google Scholar] [PubMed]
- Sebastião, C.S.; Gaston, C.; Paixão, J.P.; Sacomboio, E.N.M.; Neto, Z.; de Vasconcelos, J.N.; Morais, J. Coinfection between SARS-CoV-2 and vector-borne diseases in Luanda, Angola. J. Med. Virol. 2021, 94, 366–371. [Google Scholar] [CrossRef] [PubMed]
- Overgaard, H.J.; Sæbo, S.; Reddy, M.R.; Reddy, V.P.; Abaga, S.; Matias, A.; Slotman, M.A. Light traps fail to estimate reliable malaria mosquitobiting rates on Bioko Island, Equatorial Guinea. Malar. J. 2012, 11, 56. [Google Scholar] [CrossRef] [PubMed]
- Cuamba, N.; Choi, K.S.; Townson, H. Malaria vectors in Angola: Distribution of species and molecular forms of the Anopheles gambiae complex, their pyrethroid insecticide knockdown resistance (kdr) status and Plasmodium. Malar. J. 2006, 5, 2. [Google Scholar] [CrossRef]
- Morais, P.; Pinto, J.; Jorge, C.P.; Troco, A.D.; Fortes, F.; Sousa, C.A.; Parreira, R. Insect-specific flaviviruses and densoviruses, suggested to have been transmitted vertically, found in mosquitoes collected in Angola: Genome detection and phylogenetic characterization of viral sequences. Infect. Genet. Evol. 2020, 80, 104191. [Google Scholar] [CrossRef]
- Fernández, M.C.M.; Arletty, T.; Cani, P.; Flores, Y.H. Longitudinal spatial distribution of Aedes aegypti (Diptera: Culicidae) during the yellow fever epidemic in Angola, 2016. Glob. J. Zool. 2018, 4, 1–6. [Google Scholar] [CrossRef]
- Demanou, M.; Antonio-Nkondjio, C.; Ngapana, E.; Kouagang, E.; Nfor, C.; Ndiforchu, V.; Vola, L.S.; Tchendjou, P.; Rousset, D.; Manuguerra, J.C.; et al. Retrospective Investigation of a Dengue-Like Syndrome in a Rural Area in Western Cameroon. Int. J. Infect. Dis. 2008, 12, e62. [Google Scholar] [CrossRef]
- Nana-Ndjangwo, S.M.; Djiappi-Tchamen, B.; Mony, R.; Demanou, M.; Keumezeu-Tsafack, J.; Bamou, R.; Awono-Ambene, P.; Bilong, B.C.F.; Antonio-Nkondjio, C. Assessment of Dengue and Chikungunya Infections among Febrile Patients Visiting Four Healthcare Centres in Yaoundé and Dizangué, Cameroon. Viruses 2022, 14, 2127. [Google Scholar] [CrossRef]
- Ndip, L.M.; Bouyer, D.H.; Da Rosa, A.P.A.T.; Titanji, V.P.K.; Tesh, R.B.; Walker, D.H. Acute Spotted Fever Rickettsiosis among Febrile Patients, Cameroon. Emerg. Infect. Dis. 2004, 10, 432. [Google Scholar] [CrossRef]
- Peyrefitte, C.N.; Rousset, D.; Pastorino, B.A.M.; Pouillot, R.; Bessaud, M.; Tock, F.; Mansaray, H.; Merle, O.L.; Pascual, A.M.; Paupy, C.; et al. Chikungunya Virus, Cameroon, 2006. Emerg. Infect. Dis. 2007, 13, 768–771. [Google Scholar] [CrossRef]
- Mayi, M.P.A.; Bamou, R.; Djiappi-Tchamen, B.; Djojo-Tachegoum, C.; Fontaine, A.; Antonio-Nkondjio, C.; Tchuinkam, T. A mosquito survey along a transect of urbanization in Dschang, West Region of Cameroon, reveals potential risk of arbovirus Spillovers. bioRxiv 2019. [Google Scholar] [CrossRef]
- Sado, F.B.Y.; Simo, F.B.N.; Ngouanet, S.A.; Mekanda, F.M.O.; Demanou, M. Detection and serotyping of dengue viruses in febrile patients consulting at the New-Bell District Hospital in Douala, Cameroon. PLoS ONE 2018, 13, e0204143. [Google Scholar]
- Tchetgna, H.D.S.; Yousseu, F.B.S.; Kamgang, B.; Tedjou, A.; Mccall, P.J.; Wondj, C.S. Concurrent circulation of Dengue Serotype 1, 2, and 3 among acute febrile patients in cameroon. Int. J. Infect. Dis. 2021, 116, S125. [Google Scholar] [CrossRef]
- Techetgna, H.S.; Descorps-Declere, S.; Selekon, B.; Garba-Ouangole, S.; Konamna, X.; Berthet, N. Continuous Circulation of Yellow Fever among Rural Populations in the Central African Republic. Viruses 2022, 14, 2014. [Google Scholar] [CrossRef]
- Saluzzo, J.F.; Vincent, T.; Miller, J.; Veas, F.; Gonzalez, J.P. Arbovirus Discovery in Central African Republic (1973–1993): Zika, Bozo, Bouboui, and More. Ann. Infect. Dis. Epidemiol. 2017, 2, 1022. [Google Scholar]
- Simo, F.B.N.; Sado, F.B.Y.; Mbarga, A.E.; Bigna, J.J.; Melong, A.; Ntoude, A.; Kamgang, B.; Bouyne, R.; Fewou, P.M.; Demanou, M. Investigation of an Outbreak of Dengue Virus Serotype 1 in a Rural Area of Kribi, South Cameroon: A Cross-Sectional Study. Intervirology 2018, 61, 265–271. [Google Scholar]
- Tchuandom, S.B.; Tchouangueu, T.F.; Antonio-Nkondjio, C.; Lissom, A.; Djang, J.O.N.; Atabonkeng, E.P.; Assompta, K.; Nchinda, G.; Kuiate, J.-R. Seroprevalence of dengue virus among children presenting with febrile illness in some public health facilities in Cameroon. Pan Afr. Med. J. 2018, 31, 177. [Google Scholar] [CrossRef]
- Tchuandom, S.B.; Tchadji, J.C.; Tchouangueu, T.F.; Biloa, M.Z.; Atabonkeng, E.P.; Fumba, M.I.M.; Massom, E.S.; Nchinda, G.G.; Kuiate, J.R. A cross-sectional study of acute dengue infection in paediatric clinics in Cameroon. BMC Public Health 2019, 19, 958. [Google Scholar] [CrossRef]
- Ali, I.M.; Tchuenkam, V.P.K.; Colton, M.; Stittleburg, V.; Mitchell, C.; Gaither, C.; Thwai, K.; Espinoza, D.O.; Zhu, Y.; Jamal, H.; et al. Arboviruses as an unappreciated cause of non-malarial acute febrile illness in the Dschang Health District of western Cameroon. PLoS Negl. Trop. Dis. 2022, 16, e0010790. [Google Scholar] [CrossRef]
- Monamele, G.C.; Demanou, M. First documented evidence of dengue and malaria co-infection in children attending two health centers in Yaoundé, Cameroon. Pan Afr. Med. J. 2018, 29, 227. [Google Scholar] [CrossRef]
- Galani, B.R.T.; Mapouokam, D.W.; Simo, F.B.N.; Mohamadou, H.; Chuisseu, P.D.D.; Njintang, N.Y.; Moundipa, P.F. Investigation of dengue-malaria coinfection among febrile patients consulting at Ngaoundere Regional Hospital, Cameroon. J. Med. Virol. 2021, 93, 3350–3361. [Google Scholar] [CrossRef] [PubMed]
- Kamgang, B.; Happi, J.Y.; Boisier, P.; Njiokou, F.; Hervé, J.P.; Simard, F.; Paupy, C. Geographic and ecological distribution of the dengue and chikungunya virus vectors Aedes aegypti and Aedes albopictus in three major Cameroonian towns. Med. Vet. Entomol. 2010, 24, 132–141. [Google Scholar] [CrossRef] [PubMed]
- Tedjou, A.N.; Kamgang, B.; Yougang, A.P.; Wilson-Bahun, T.A.; Njiokou, F.; Wondji, C.S. Patterns of Ecological Adaptation of Aedes aegypti and Aedes albopictus and Stegomyia Indices Highlight the Potential Risk of Arbovirus Transmission in Yaoundé, the Capital City of Cameroon. Pathogens 2020, 9, 491. [Google Scholar] [CrossRef]
- Hondt, O.E.N.; Ntonga, P.A.; Hiol, J.V.N.; Edou, D.N.; Tonga, C.; Dadji, G.A.F.; Kekeunou, S. Adaptation compétitive d’Aedes albopictus Skuse, 1894 en présence d’Aedes aegypti Linné, 1862 dans quelques gîtes larvaires temporaires de la ville de Douala (Cameroun) dans un contexte de résistance aux pyréthrinoïdes. Bull. Soc. Pathol. Exot. 2020, 113, 79–87. [Google Scholar] [CrossRef] [PubMed]
- Djiappi-Tchamen, B.; Nana-Ndjangwo, M.S.; Nchoutpouen, E.; Makoudjou, I.; Ngangue-Siewe, I.N.; Talipouo, A.; Mayi, M.P.A.; Awono-Ambene, P.; Wondji, C.; Tchuinkam, T.; et al. Aedes Mosquito Surveillance Using Ovitraps, Sweep Nets, and Biogent Traps in the City of Yaoundé, Cameroon. Insects 2022, 13, 793. [Google Scholar] [CrossRef] [PubMed]
- Demanou, M.; Ratsitoharana, R.; Seukap, E.; Abassora, M.; Nimpa, M.; Onambay, C.; Gake, B.; Tabonfack, A.; Faye, O.; Sall, A.; et al. Yellow fever outbreak in North Cameroon, 2011. Conference Paper. January 2011. Available online: https://www.researchgate.net/publication/331089142 (accessed on 10 October 2023).
- Nemg, F.B.S.; Abanda, N.N.; Yonga, M.G.; Ouapi, D.; Samme, I.E.; Djoumetio, M.D.; Endegue-Zanga, M.C.; Demanou, M.; Njouom, R. Sustained circulation of yellow fever virus in Cameroon: An analysis of laboratory surveillance data, 2010–2020. BMC Infect. Dis. 2022, 22, 418. [Google Scholar] [CrossRef] [PubMed]
- Gake, B.; Vernet, M.A.; Leparc-Goffart, I.; Drexler, J.F.; Goulda, E.A.; Galliana, P.; de Lamballerie, X. Low seroprevalence of Zika virus in Cameroonian blood donors. Braz. J. Infect. Dis. 2017, 21, 481–483. [Google Scholar] [CrossRef]
- Kamgang, B.; Vazeille, M.; Tedjou, A.; Yougang, A.P.; Wilson-Bahun, T.A.; Mousson, L.; Wondji, C.S.; Failloux, A.-B. Different populations of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) from Central Africa are susceptible to Zika virus infection. PLoS Negl. Trop. Dis. 2020, 14, e0008163. [Google Scholar] [CrossRef]
- Namegni, R.S.P.; Njan-Nloga, A.M.; Wade, A.; Eisenbarth, A.; Groschup, M.H.; Stoek, F. Diversity and Abundance of Potential Vectors of Rift Valley Fever Virus in the North Region of Cameroon. Insects 2020, 11, 814. [Google Scholar]
- Staples, J.E.; Diallo, M.; Janusz, K.B.; Manengu, C.; Lewis, R.F.; Perea, W.; Yactayo, S.; Sallb, A.A.; RCA Risk Assessment Team. Yellow fever risk assessment in the Central African Republic. Trans. R. Soc. Trop. Med. Hyg. 2010, 108, 608–615. [Google Scholar] [CrossRef]
- Mathiot, C.C.; Gonzalez, J.P.; Georges, A.J. Current problems of arboviruses in central Africa. Bull. Soc. Pathol. Exot. Fil. 1988, 81, 396–401. [Google Scholar]
- Tricou, V.; Desdouits, M.; Nakouné, E.; Gessain, A.; Kazanji, M.; Berthet, N. Complete genome sequences of two chikungunya viruses isolated in the Central African Republic in the 1970s and 1980s. Genome Announc. 2017, 5, e00003-17. [Google Scholar] [CrossRef] [PubMed]
- Saluzzo, J.F.; Gonzalez, J.P.; Hervé, J.P.; Georges, A.J. Serological survey for the prevalence of certain arboviruses in the human population of the south-east area of Central African Republic. Bull. Soc. Pathol. Exot. Fil. 1981, 74, 490–499. [Google Scholar]
- Desdouits, M.; Kamgang, B.; Berthet, N.; Tricou, V.; Ngoagouni, C.; Gessain, A.; Manuguerra, J.-C.; Nakouné, E.; Kazanji, M. Genetic characterization of Chikungunya virus in the Central African Republic. Infect. Genet. Evol. 2015, 33, 25–31. [Google Scholar] [CrossRef] [PubMed]
- Ngoagouni, C.; Kamgang, B.; Brengues, C.; Yahouedo, G.; Paupy, C.; Nakouné, E.; Kazanji, M.; Fabrice, C.F. Susceptibility profile and metabolic mechanisms involved in Aedes aegypti and Aedes albopictus resistant to DDT and deltamethrin in the Central African Republic. Parasites Vectors 2016, 9, 599. [Google Scholar] [CrossRef]
- Ngoagouni, C.; Kamgang, B.; Kazanji, M.; Paupy, C.; Nakouné, E. Potential of Aedes aegypti and Aedes albopictus populations in the Central African Republic to transmit enzootic chikungunya virus strains. Parasites Vectors 2017, 10, 164. [Google Scholar] [CrossRef]
- Diarra, A.Z.; Laroche, M.; Berger, F.; Parola, P. Use of MALDI-TOF MS for the Identification of Chad Mosquitoes and the Origin of Their Blood Meal. Am. J. Trop. Med. Hyg. 2019, 100, 47–53. [Google Scholar] [CrossRef]
- Nakouné, E.; Kamgang, B.; Berthet, N.; Manirakiza, A.; Kazanji, M. Rift Valley Fever Virus Circulating among Ruminants, Mosquitoes and Humans in the Central African Republic. PLoS Negl. Trop. Dis. 2016, 10, e0005082. [Google Scholar] [CrossRef]
- Guilherm, J.M.; Gonella-Legall, C.; Legall, F.; Nanouke, E.; Vincent, J. Seroprevalence of five arboviruses in Zebu cattle in the Central African Republic. Trans. R. Soc. Trop. Med. Hyg. 1996, 90, 31–33. [Google Scholar] [CrossRef]
- Bessimbaye, N.; Mbanga, D.; Moussa, A.M.; Ferdinand, D.Y.; Maxime, N.D.; Barro, N.; Tidjani, A.; Ouchemi, C. Evaluation of yellow fever surveillance in Chad, 2015–2020. Afr. J. Microbiol. Res. 2021, 15, 152–160. [Google Scholar]
Figure 1.
Flow chart of the steps in the reviewing process.
Figure 2.
Number of articles retained by each country from 1993 to 2023.
Figure 3.
The periodic trend in the publication of entomological and epidemiological data by country.
Figure 3.
The periodic trend in the publication of entomological and epidemiological data by country.
Figure 4.
Genera of mosquitoes identified in the Central African subregion from January 1993 to June 2023; (**) unidentified species.
Figure 4.
Genera of mosquitoes identified in the Central African subregion from January 1993 to June 2023; (**) unidentified species.
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Poungou, N.; Sevidzem, S.L.; Koumba, A.A.; Koumba, C.R.Z.; Mbehang, P.; Onanga, R.; Zahouli, J.Z.B.; Maganga, G.D.; Djogbénou, L.S.; Borrmann, S.; et al. Mosquito-Borne Arboviruses Occurrence and Distribution in the Last Three Decades in Central Africa: A Systematic Literature Review. Microorganisms 2024, 12, 4. https://doi.org/10.3390/microorganisms12010004
AMA Style
Poungou N, Sevidzem SL, Koumba AA, Koumba CRZ, Mbehang P, Onanga R, Zahouli JZB, Maganga GD, Djogbénou LS, Borrmann S, et al. Mosquito-Borne Arboviruses Occurrence and Distribution in the Last Three Decades in Central Africa: A Systematic Literature Review. Microorganisms. 2024; 12(1):4. https://doi.org/10.3390/microorganisms12010004
Chicago/Turabian StylePoungou, Natacha, Silas Lendzele Sevidzem, Aubin Armel Koumba, Christophe Roland Zinga Koumba, Phillipe Mbehang, Richard Onanga, Julien Zahouli Bi Zahouli, Gael Darren Maganga, Luc Salako Djogbénou, Steffen Borrmann, and et al. 2024. "Mosquito-Borne Arboviruses Occurrence and Distribution in the Last Three Decades in Central Africa: A Systematic Literature Review" Microorganisms 12, no. 1: 4. https://doi.org/10.3390/microorganisms12010004
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