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Open AccessArticle
Exploring the Spatial Variation in the Microbiota and Bile Acid Metabolism of the Compound Stomach in Intensively Farmed Yaks
by
Shichun He
Shichun He 1,†,
Zaimei Yuan
Zaimei Yuan 2,†,
Sifan Dai
Sifan Dai 1,
Zibei Wang
Zibei Wang 1,
Shusheng Zhao
Shusheng Zhao 1,
Bin Zhang
Bin Zhang 3,
Huaming Mao
Huaming Mao 1,* and
Dongwang Wu
Dongwang Wu 1,*
1
Yunnan Provincial Key Laboratory of Animal Nutrition and Feed, Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China
2
Kunming Animal Disease Prevention and Control Center, Kunming 650106, China
3
Yunnan Academy of Animal Husbandry and Veterinary Sciences, Kunming 650224, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Microorganisms 2024, 12(10), 1968; https://doi.org/10.3390/microorganisms12101968 (registering DOI)
Submission received: 3 September 2024
/
Revised: 24 September 2024
/
Accepted: 26 September 2024
/
Published: 28 September 2024
Abstract
Yaks are one of the important livestock on the Qinghai–Tibet Plateau, providing abundant dairy and meat products for the local people. The formation of these dairy and meat products mainly relies on the microbiota in their gastrointestinal tract, which digests and metabolizes plant feed. The yak’s gastrointestinal microbiota is closely related to the health and production performance of the host, but the molecular mechanisms of diet-induced effects in intensively farmed yaks remain to be elucidated. In this study, 40 chyme samples were collected from the four stomach chambers of 10 intensively farmed yaks, and the bacterial diversity and bile acid changes in the rumen (SFRM), reticulum (SFRC), omasum (SFOM), and abomasum (SFAM) were systematically analyzed using 16S rRNA sequencing and bile acid metabolism. Our results showed that the gastrointestinal microbiota mainly distributes in the four-chambered stomach, with the highest microbial diversity in the reticulum. There is a highly negative correlation among the microbiota in the four chambers. The dominant bacterial phyla, Bacteroidota and Firmicutes, were identified, with Rikenellaceae_RC9_gut_group being the dominant genus, which potentially helps maintain short-chain fatty acid levels in the stomach. In contrast, the microbiome within the four stomach chambers synergistically and selectively altered the content and diversity of bile acid metabolites in response to intensive feeding. The results of this study provide new insights into the microbiota and bile acid metabolism functions in the rumen, reticulum, omasum, and abomasum of yaks. This can help uncover the role of gastrointestinal microbiota in yak growth and metabolic regulation, while also providing references for improving the production efficiency and health of ruminants.
Share and Cite
MDPI and ACS Style
He, S.; Yuan, Z.; Dai, S.; Wang, Z.; Zhao, S.; Zhang, B.; Mao, H.; Wu, D.
Exploring the Spatial Variation in the Microbiota and Bile Acid Metabolism of the Compound Stomach in Intensively Farmed Yaks. Microorganisms 2024, 12, 1968.
https://doi.org/10.3390/microorganisms12101968
AMA Style
He S, Yuan Z, Dai S, Wang Z, Zhao S, Zhang B, Mao H, Wu D.
Exploring the Spatial Variation in the Microbiota and Bile Acid Metabolism of the Compound Stomach in Intensively Farmed Yaks. Microorganisms. 2024; 12(10):1968.
https://doi.org/10.3390/microorganisms12101968
Chicago/Turabian Style
He, Shichun, Zaimei Yuan, Sifan Dai, Zibei Wang, Shusheng Zhao, Bin Zhang, Huaming Mao, and Dongwang Wu.
2024. "Exploring the Spatial Variation in the Microbiota and Bile Acid Metabolism of the Compound Stomach in Intensively Farmed Yaks" Microorganisms 12, no. 10: 1968.
https://doi.org/10.3390/microorganisms12101968
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