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The Anti-Constipation Effect of Bifidobacterium Longum W11 Is Likely Due to a Key Genetic Factor Governing Arabinan Utilization

Microorganisms 2024, 12(8), 1626; https://doi.org/10.3390/microorganisms12081626
by Francesco Di Pierro 1,2,3,*, Nicola Zerbinati 3, Massimiliano Cazzaniga 2, Alexander Bertuccioli 1,4, Chiara Maria Palazzi 1, Ilaria Cavecchia 1,5, Mariarosaria Matera 1,6, Edoardo Labrini 7, Valeria Sagheddu 7 and Sara Soldi 7
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Microorganisms 2024, 12(8), 1626; https://doi.org/10.3390/microorganisms12081626
Submission received: 21 July 2024 / Revised: 7 August 2024 / Accepted: 7 August 2024 / Published: 9 August 2024
(This article belongs to the Special Issue Probiotics, Prebiotics, and Gut Microbes)

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

This is a very specialized research, aiming  to document the  existence of two of the three genes encoding arabinofuranosidases, the abfA and abfB in Bifidobacterium longum W11, known to ameliorate functional constipation.

These results open the way for similar search in other strains as to whether they are ably to metabolize arabinan through the expression of these genes and thus positive implicated in the alleviation of constipation.

Minor comment

although there are references in irritable bower syndrome, I would suggest authors to remain to simply "functional constipation" [para 1, line 15], since in IBD other psychosomatic mechanisms theoretically are implicated, too

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Reviewer 2 Report

Comments and Suggestions for Authors

The manuscript by Francesco Di Pierro et al. is largely a repeated work of "A key genetic factor governing arabinan utilization in the gut microbiome alleviates constipation" published in Cell Host Microbe. I have not seen any new ideas and new findings in it. I suggest to reject it. 

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

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