Tracing Acinetobacter baumannii’s Journey from Hospitals to Aquatic Ecosystems
Abstract
:1. Introduction
2. Materials and Methods
2.1. Water Sampling Campaign
2.2. Strain Isolation, Quantification, Identification, and Antimicrobial Susceptibility Profiles
2.3. Characterization of Genotypic Resistance Profiles
2.4. Whole Genome Sequencing (WGS) and Bioinformatic Analyses of Clinical and Aquatic A. baumannii Isolates
2.5. Collection of Fish Samples
2.6. Metagenomic Analysis of Surface Water Samples and Fish Microbiota to Highlight the Connection between the Environment and Fish Microbiota
3. Results
3.1. Phenotypic and Genotypic AR Profiles of A. baumannii Isolates
3.1.1. Isolation and Quantification of A. baumannii from Romanian Wastewater and Surface Water Samples
- ESBL phenotype: EF Glina, Bucharest > IN Glina, Bucharest > IN Târgoviște >AS Glina, Bucharest > EF Târgoviște.
- CARBA phenotype: EF Glina, Bucharest > IN Glina, Bucharest > IN Târgoviște >AS Glina, Bucharest > EF Târgoviște.
- total Acinetobacter phenotype: IN Glina, Bucharest > IN Târgoviște > AS Glina, Bucharest > EF Glina, Bucharest > EF Târgoviște (Figure 2 and Supplementary Table S4).
3.1.2. Antimicrobial Susceptibilities Profiles of A. baumannii Isolates from Different Isolation Sources and by Geographical Location
3.1.3. Genotypic Characterization of β-Lactam Resistance in Clinical, Wastewater, and Surface Water A. baumannii Isolates
3.1.4. WGS Analysis in Clinical, Wastewater, and Surface Water A. baumannii Isolates
3.2. Metagenomic Analysis of Surface Water Samples and Fish Microbiota to Highlight the Connection between the Environment and Fish Microbiota
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Antibiotic/ Isolate | 22012-CA5 | 22012-ENE6 | 22013-CA5 | 22013-ENE4 | 22014-CA2 | 22014-COLN5 | 22015-CA3 | 22015-CA4 | 22015-ENE6 | 22016-CA2 | 22016-CNE3 | 22016-CNE4 | 22017-CNE1 | 22018-CA5 | 22018-CA6 | 22019-CNE4 | 22019-CNE5 | 24-IHI | 3-IHI | 49-IHI |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Colistin (µG/ML) | 0.25 | <0.25 | <0.25 | <0.25 | <0.25 | <0.25 | <0.25 | <0.25 | <0.25 | <0.25 | <0.25 | <0.25 | <0.25 | <0.25 | <0.25 | <0.25 | <0.25 | 1 | <0.25 | <0.25 |
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Gheorghe-Barbu, I.; Dragomir, R.-I.; Gradisteanu Pircalabioru, G.; Surleac, M.; Dinu, I.A.; Gaboreanu, M.D.; Czobor Barbu, I. Tracing Acinetobacter baumannii’s Journey from Hospitals to Aquatic Ecosystems. Microorganisms 2024, 12, 1703. https://doi.org/10.3390/microorganisms12081703
Gheorghe-Barbu I, Dragomir R-I, Gradisteanu Pircalabioru G, Surleac M, Dinu IA, Gaboreanu MD, Czobor Barbu I. Tracing Acinetobacter baumannii’s Journey from Hospitals to Aquatic Ecosystems. Microorganisms. 2024; 12(8):1703. https://doi.org/10.3390/microorganisms12081703
Chicago/Turabian StyleGheorghe-Barbu, Irina, Rares-Ionut Dragomir, Gratiela Gradisteanu Pircalabioru, Marius Surleac, Iulia Adelina Dinu, Madalina Diana Gaboreanu, and Ilda Czobor Barbu. 2024. "Tracing Acinetobacter baumannii’s Journey from Hospitals to Aquatic Ecosystems" Microorganisms 12, no. 8: 1703. https://doi.org/10.3390/microorganisms12081703