Biocontrol Potential of Bacillus stercoris Strain DXQ-1 Against Rice Blast Fungus Guy11

Xu, Qian; Shan, Zhengli; Yang, Zhihao; Ma, Haoyu; Zou, Lijuan; Dong, Ming; Qi, Tuo

doi:10.3390/microorganisms13071538

This is an early access version, the complete PDF, HTML, and XML versions will be available soon.

Open AccessArticle

Biocontrol Potential of Bacillus stercoris Strain DXQ-1 Against Rice Blast Fungus Guy11

by

Qian Xu

,

Zhengli Shan

,

Zhihao Yang

,

Haoyu Ma

,

Lijuan Zou

,

Ming Dong

and

Tuo Qi

^*

Ecological Security and Protection Key Laboratory of Sichuan Province, Mianyang Teachers’ College, Mianyang 621000, China

^*

Author to whom correspondence should be addressed.

Microorganisms 2025, 13(7), 1538; https://doi.org/10.3390/microorganisms13071538

Submission received: 26 May 2025 / Revised: 19 June 2025 / Accepted: 27 June 2025 / Published: 30 June 2025

(This article belongs to the Section Plant Microbe Interactions)

Download Review Reports Versions Notes

Abstract

Fungal diseases severely threaten global agriculture, while conventional chemical fungicides face increasing restrictions due to environmental and safety concerns. In this study, we isolated a soil-derived Bacillus stercoris strain, DXQ-1, exhibiting strong antagonistic activity against plant pathogenic fungi, notably Magnaporthe oryzae, the causal agent of rice blast. Scanning electron microscopy revealed that DXQ-1 disrupts fungal hyphae and inhibits conidial germination, with a 24 h crude broth treatment reducing germination to 83.33% and completely blocking appressoria formation. LC-MS-based metabolomic analysis identified key antifungal components, including lipids (35.83%), organic acid derivatives (22.15%), and small bioactive molecules (e.g., Leu-Pro, LPE 15:0). After optimizing fermentation conditions (LB medium, pH 7.0, 28 °C, 48 h), the broth showed >90% inhibition against M. oryzae and Nigrospora oryzae and retained high thermal (68 °C, 1 h) and UV (4 h) stability. Field trials demonstrated effective disease control and significant promotion of rice growth, increasing plant height (17.7%), fresh weight (53.3%), and dry weight (33.3%). These findings highlight DXQ-1 as a promising biocontrol agent, offering a sustainable and effective alternative for managing fungal diseases in crops.

Keywords: Bacillus; fungal pathogen; plant disease control; Magnaporthe oryzae; antifungal metabolites

Share and Cite

MDPI and ACS Style

Xu, Q.; Shan, Z.; Yang, Z.; Ma, H.; Zou, L.; Dong, M.; Qi, T. Biocontrol Potential of Bacillus stercoris Strain DXQ-1 Against Rice Blast Fungus Guy11. Microorganisms 2025, 13, 1538. https://doi.org/10.3390/microorganisms13071538

AMA Style

Xu Q, Shan Z, Yang Z, Ma H, Zou L, Dong M, Qi T. Biocontrol Potential of Bacillus stercoris Strain DXQ-1 Against Rice Blast Fungus Guy11. Microorganisms. 2025; 13(7):1538. https://doi.org/10.3390/microorganisms13071538

Chicago/Turabian Style

Xu, Qian, Zhengli Shan, Zhihao Yang, Haoyu Ma, Lijuan Zou, Ming Dong, and Tuo Qi. 2025. "Biocontrol Potential of Bacillus stercoris Strain DXQ-1 Against Rice Blast Fungus Guy11" Microorganisms 13, no. 7: 1538. https://doi.org/10.3390/microorganisms13071538

APA Style

Xu, Q., Shan, Z., Yang, Z., Ma, H., Zou, L., Dong, M., & Qi, T. (2025). Biocontrol Potential of Bacillus stercoris Strain DXQ-1 Against Rice Blast Fungus Guy11. Microorganisms, 13(7), 1538. https://doi.org/10.3390/microorganisms13071538

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Menu

Biocontrol Potential of Bacillus stercoris Strain DXQ-1 Against Rice Blast Fungus Guy11

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Further Information

Guidelines

MDPI Initiatives

Follow MDPI