Dynamic Succession of Microbiota during Ensiling of Whole Plant Corn Following Inoculation with Lactobacillus buchneri and Lactobacillus hilgardii Alone or in Combination
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ensiling and Experimental Treatments
2.2. Fermentation Profiles and Physical Characterization
2.3. DNA Extraction
2.4. High-Throughput Sequencing and Bioinformatics Analysis
2.5. Statistical Analysis
3. Results and Discussion
3.1. Eubacterial Succession over the Incubation Period
3.2. Fungal Succession during the Course of the Incubation Period
3.3. Fermentation Profiling
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Treatments | pH a | Total VFA a | Lactic Acid a | Acetic Acid a | Ratio Lactate/(Acetate + EtOH) a |
---|---|---|---|---|---|
Lact. buchneri 40788 | 0.855 | 0.972 | 0.011 | <0.001 | <0.001 |
Lact. hilgardii 4785 | 0.736 | 0.206 | 0.410 | <0.001 | <0.001 |
Combo LB–LH | 0.552 | 0.396 | 0.109 | <0.001 | <0.001 |
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Drouin, P.; Tremblay, J.; Chaucheyras-Durand, F. Dynamic Succession of Microbiota during Ensiling of Whole Plant Corn Following Inoculation with Lactobacillus buchneri and Lactobacillus hilgardii Alone or in Combination. Microorganisms 2019, 7, 595. https://doi.org/10.3390/microorganisms7120595
Drouin P, Tremblay J, Chaucheyras-Durand F. Dynamic Succession of Microbiota during Ensiling of Whole Plant Corn Following Inoculation with Lactobacillus buchneri and Lactobacillus hilgardii Alone or in Combination. Microorganisms. 2019; 7(12):595. https://doi.org/10.3390/microorganisms7120595
Chicago/Turabian StyleDrouin, Pascal, Julien Tremblay, and Frédérique Chaucheyras-Durand. 2019. "Dynamic Succession of Microbiota during Ensiling of Whole Plant Corn Following Inoculation with Lactobacillus buchneri and Lactobacillus hilgardii Alone or in Combination" Microorganisms 7, no. 12: 595. https://doi.org/10.3390/microorganisms7120595