Rapid Changes in Microbial Community Structures along a Meandering River
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Area
2.2. Sampling Sites and Methods
2.3. DNA Extraction, DNA Amplification, and Sequencing
2.4. Sequencing Analyses
2.5. Quantitative Real-Time PCR
2.6. Physicochemical Parameters
2.7. Statistical Analyses
3. Results
3.1. Environmental Parameters
3.2. Microbial Community Structures
3.3. Presence/Absence of OTUs
3.4. Correlations between Bacterial OTUs, Eukaryotic OTUs, and Environmental Parameters
3.5. Variations in the Relative Proportions of the Main Bacterial Taxa
3.6. Variations in the Relative Proportions of the Main Eukaryotic Taxa
3.7. Quantitative Real-Time PCR
4. Discussion
4.1. Progressive Changes of the Global Microbial Community along the River Course
4.2. Contrasted Dynamics among Microbial Communities
4.3. Fall of the Lake Microbial Communities
4.4. Rise of the Riverine Microbial Communities
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Cruaud, P.; Vigneron, A.; Dorea, C.C.; Rodriguez, M.J.; Charette, S.J. Rapid Changes in Microbial Community Structures along a Meandering River. Microorganisms 2020, 8, 1631. https://doi.org/10.3390/microorganisms8111631
Cruaud P, Vigneron A, Dorea CC, Rodriguez MJ, Charette SJ. Rapid Changes in Microbial Community Structures along a Meandering River. Microorganisms. 2020; 8(11):1631. https://doi.org/10.3390/microorganisms8111631
Chicago/Turabian StyleCruaud, Perrine, Adrien Vigneron, Caetano C. Dorea, Manuel J. Rodriguez, and Steve J. Charette. 2020. "Rapid Changes in Microbial Community Structures along a Meandering River" Microorganisms 8, no. 11: 1631. https://doi.org/10.3390/microorganisms8111631