The HIV-1 Transgenic Rat: Relevance for HIV Noninfectious Comorbidity Research
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Tissue Processing
2.3. Immunocytochemistry for Transgene Products gp120 and Tat
2.4. Immunochemistry for CXCR4
2.5. Special Stain
2.6. Routine Stain
2.7. Slide Review of H&E Serial Sections
2.8. Slide Review Silver Stain
2.9. Slide Review of CXCR4 Immunostaining
2.10. Imaging
3. Results
3.1. Cortical Pathology
3.2. Hippocampal Pathology
3.3. Striatal Pathology
3.4. Immunohistochemical Identification of gp120 and Tat in the Brain
3.5. Spleen Pathology
3.5.1. Immunohistochemical Identification of gp120 and Tat
3.5.2. Identification of CXCR4 in the Spleen
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Denaro, F.; Benedetti, F.; Worthington, M.D.; Scapagnini, G.; Krauss, C.C.; Williams, S.; Bryant, J.; Davis, H.; Latinovic, O.S.; Zella, D. The HIV-1 Transgenic Rat: Relevance for HIV Noninfectious Comorbidity Research. Microorganisms 2020, 8, 1643. https://doi.org/10.3390/microorganisms8111643
Denaro F, Benedetti F, Worthington MD, Scapagnini G, Krauss CC, Williams S, Bryant J, Davis H, Latinovic OS, Zella D. The HIV-1 Transgenic Rat: Relevance for HIV Noninfectious Comorbidity Research. Microorganisms. 2020; 8(11):1643. https://doi.org/10.3390/microorganisms8111643
Chicago/Turabian StyleDenaro, Frank, Francesca Benedetti, Myla D. Worthington, Giovanni Scapagnini, Christopher C. Krauss, Sumiko Williams, Joseph Bryant, Harry Davis, Olga S. Latinovic, and Davide Zella. 2020. "The HIV-1 Transgenic Rat: Relevance for HIV Noninfectious Comorbidity Research" Microorganisms 8, no. 11: 1643. https://doi.org/10.3390/microorganisms8111643