Chemical Ecology of Streptomyces albidoflavus Strain A10 Associated with Carpenter Ant Camponotus vagus
Abstract
:1. Introduction
2. Materials and Methods
2.1. Collection and Microbial Isolation
2.2. Phenotypic Characterization
2.3. Gene Amplification and Phylogenetic Analysis
2.4. Fermentation and Antimicrobial Assay
2.5. Isolation and Identification of Antimycins
3. Results
3.1. Isolation and Characterization of Streptomyces Strain A10 Associated with Carpenter Ants
3.2. Antimicrobial Properties of Streptomyces sp. A10
3.3. Identification of Antifungal Compounds Produced by Strain A10
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Strains | 1 | 2 | 3 | 4 |
---|---|---|---|---|
Yeast extract-malt extract agar (ISP-2) | ||||
Growth | good | good | good | good |
Aerial mycelium | greenish beige | sparse, white | white yellow | grey |
Substrate mycelium | pale yellow | colorless | brown | ivory |
Diffusible pigment | none | none | none | none |
Oatmeal agar (ISP-3) | ||||
Growth | good | good | good | good |
Aerial mycelium | greenish beige | none | white yellow | grey |
Substrate mycelium | light yellow | colorless | brown | ivory |
Diffusible pigment | none/reddish | none/reddish | none | none |
Inorganic salts-starch agar (ISP-4) | ||||
Growth | moderate | good | good | good |
Aerial mycelium | none | sparse, white | white yellow | grey |
Substrate mycelium | pale yellow | colorless | brown | ivory |
Diffusible pigment | none | none | none | none |
Glycerol-asparagine agar (ISP-5) | ||||
Growth | good | good | weak | good |
Aerial mycelium | beige | white | white yellow | white/yellow |
Substrate mycelium | yellowish white | colorless | creamy | yellow |
Diffusible pigment | none | none | none | none |
Peptone-yeast extract-iron agar (ISP-6) | ||||
Growth | good | good | good | n/d |
Aerial mycelium | slightly pink | white | white/grey | n/d |
Substrate mycelium | brownish pink | brown | dark brown | n/d |
Diffusible pigment | none | brown | dark brown | n/d |
Tyrosine agar (ISP-7) | ||||
Growth | good | good | good | n/d |
Aerial mycelium | beige | sparse, white | white | n/d |
Substrate mycelium | slightly pink | colorless | brown | n/d |
Diffusible pigment | none | none | brown | n/d |
Phenotypic Tests | 1 | 2 | 3 | 4 |
---|---|---|---|---|
Morphology spore chains | RF | RF | RF | RF |
Spore surface | smooth | smooth | smooth | smooth |
Melanoid pigment | - | - | + | - |
Growth: | ||||
1% NaCl | + | + | + | + |
5% NaCl | + | + | + | + |
8% NaCl | + | w | + | n/d |
10 °C | + | n/d | + | n/d |
45 °C | w | n/d | n/d | n/d |
Acid from: | ||||
Adonitol | - | n/d | - | n/d |
Arabinose | - | -/+ | + | + |
Dulcit | - | n/d | n/d | n/d |
Fructose | + | + | + | - |
Galactose | + | + | n/d | n/d |
Glucose | + | + | n/d | + |
Inositol | - | - | - | - |
Lactose | - | n/d | n/d | n/d |
Maltose | + | n/d | n/d | n/d |
Mannitol | + | - | + | - |
Mannose | w | + | n/d | n/d |
Raffinose | - | - | - | - |
Rhamnose | - | - | - | - |
Salicin | - | n/d | n/d | n/d |
Sorbitol | - | n/d | n/d | n/d |
Sucrose | w | w | - | - |
Xylose | + | + | + | - |
Enzymes: | ||||
L-arginine | + | + | n/d | n/d |
L-ornitin | + | + | n/d | n/d |
L-lysin | + | + | n/d | n/d |
Decomposition of: | ||||
Cellulose | - | - | - | - |
Gelatin | + | + | + | n/d |
Starch | + | + | + | n/d |
Urea | + | + | n/d | n/d |
Production of: | ||||
Soluble pigments | + | + | + | n/d |
Citrate utilization | + | + | n/d | n/d |
Malonate utilization | + | n/d | n/d | n/d |
β-glucosidase | - | n/d | n/d | n/d |
Test Strain | Growth Inhibition Rate (%) * from Dual-Culture Assay | Growth Inhibition from Cross Streak Assay |
---|---|---|
O. sinensis VKPM F-1479 | 5.0 ± 3.2 b | - |
C. coronatus VKPM F-1359 | 15.7 ± 1.8 a | + |
B. bassiana VKPM F-1357 | 18.2 ± 3.6 b | + |
C. coronatus VKPM F-442 | 28.9 ± 3.4 a | ++ |
M. rileyi VKPM F-1360 | n/a | +++ |
L. lecanii VKPM F-837 | n/a | - |
C. albicans CBS 8836 | n/a | +++ |
A. niger INA 00760 | n/a | - |
St. aureus ATCC 25923 | n/a | - |
B. subtilis ATCC 6633 | n/a | - |
B. thuringiensis VKPM B-6650 | n/a | - |
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Baranova, A.A.; Chistov, A.A.; Tyurin, A.P.; Prokhorenko, I.A.; Korshun, V.A.; Biryukov, M.V.; Alferova, V.A.; Zakalyukina, Y.V. Chemical Ecology of Streptomyces albidoflavus Strain A10 Associated with Carpenter Ant Camponotus vagus. Microorganisms 2020, 8, 1948. https://doi.org/10.3390/microorganisms8121948
Baranova AA, Chistov AA, Tyurin AP, Prokhorenko IA, Korshun VA, Biryukov MV, Alferova VA, Zakalyukina YV. Chemical Ecology of Streptomyces albidoflavus Strain A10 Associated with Carpenter Ant Camponotus vagus. Microorganisms. 2020; 8(12):1948. https://doi.org/10.3390/microorganisms8121948
Chicago/Turabian StyleBaranova, Anna A., Alexey A. Chistov, Anton P. Tyurin, Igor A. Prokhorenko, Vladimir A. Korshun, Mikhail V. Biryukov, Vera A. Alferova, and Yuliya V. Zakalyukina. 2020. "Chemical Ecology of Streptomyces albidoflavus Strain A10 Associated with Carpenter Ant Camponotus vagus" Microorganisms 8, no. 12: 1948. https://doi.org/10.3390/microorganisms8121948