Transcriptional Regulation of the Outer Membrane Protein A in Acinetobacter baumannii
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains, Plasmids, and Culture Conditions
2.2. Construction of the ΔA1S_0316 Mutant Strain
2.3. Construction of a ΔA1S_0316 Complementary Strain Using pWH1266:A1S_0316
2.4. Construction of pB4:A1S_0316
2.5. Construction of pSA508:AbOmpAp for the In Vitro Transcription Assay
2.6. Primer Extension Assay
2.7. DNA Affinity Chromatography
2.8. Purification of A1S_0316
2.9. Electrophoretic Mobility Shift Assay (EMSA)
2.10. Real-Time qPCR
2.11. Western Blotting of the Bacterial Membrane Fraction
2.12. In Vitro Transcription Assay
3. Results
3.1. Characterization of the OmpA Promoter Region in A. baumannii
3.2. Identification of Proteins that Bind to the OmpA Promoter
3.3. Molecular Features of A1S_0316
3.4. Effect of A1S_0316 and AbH-NS on OmpA Expression In Vivo
3.5. Effect of A1S_0316 and AbH-NS on OmpA Expression In Vitro
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Oh, K.-W.; Kim, K.; Islam, M.M.; Jung, H.-W.; Lim, D.; Lee, J.C.; Shin, M. Transcriptional Regulation of the Outer Membrane Protein A in Acinetobacter baumannii. Microorganisms 2020, 8, 706. https://doi.org/10.3390/microorganisms8050706
Oh K-W, Kim K, Islam MM, Jung H-W, Lim D, Lee JC, Shin M. Transcriptional Regulation of the Outer Membrane Protein A in Acinetobacter baumannii. Microorganisms. 2020; 8(5):706. https://doi.org/10.3390/microorganisms8050706
Chicago/Turabian StyleOh, Kyu-Wan, Kyeongmin Kim, Md. Maidul Islam, Hye-Won Jung, Daejin Lim, Je Chul Lee, and Minsang Shin. 2020. "Transcriptional Regulation of the Outer Membrane Protein A in Acinetobacter baumannii" Microorganisms 8, no. 5: 706. https://doi.org/10.3390/microorganisms8050706