Haplotype Diversity of NADPH-Cytochrome P450 Reductase Gene of Ophiocordyceps sinensis and the Effect on Fungal Infection in Host Insects
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sampling
2.2. DNA Extraction and Sequencing
2.3. Data Analyses
3. Results
3.1. Gene Sequence Characteristics and Protein Structures
3.2. Haplotypes Diversity of NADPH CPR Genes
3.3. Functional Analysis of NADPH CPR
3.4. Correspondence between O. sinensis and Its Host Insects
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Position | H1 | H5 | H8 | H14 | H16 | H17 | H28 |
---|---|---|---|---|---|---|---|
335 | T | T | - | T | - | T | T |
397 | T | - | - | T | - | - | - |
401 | S | - | - | S | - | - | - |
425 | - | S | S | - | S | S | S |
443 | - | T | T | - | T | T | T |
454 | - | S | S | - | S | S | S |
636 | T | T | T | - | - | T | - |
Position | H1 | H4 | H10 | H12 | H29 |
---|---|---|---|---|---|
23 | T | - | - | - | - |
24 | T | - | - | - | - |
25 | S | - | - | - | - |
189 | - | S | S | S | S |
252 | S | - | - | - | - |
257 | - | T | T | T | T |
437 | - | Y | Y | Y | - |
539 | - | Y | Y | Y | - |
777 | S | - | S | S | S |
881 | S | S | - | - | S |
Functional Site | H1 | H5 | H8 | H14 | H16 | H17/H28 | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Position | Sequence | Position | Sequence | Position | Sequence | Position | Sequence | Position | Sequence | Position | Sequence | |
N-gly | 167–170 | NNTY | 167–170 | NNTY | 167–170 | NNTY | 167–170 | NNTY | 167–170 | NNTY | 167–170 | NNTY |
244–247 | NLTR | 244–247 | NLTR | 244–247 | NLTR | 244–247 | NLTR | 244–247 | NLTR | 244–247 | NLTR | |
305–308 | NLTY | 305–308 | NLTY | 305–308 | NLTY | 305–308 | NLTY | 305–308 | NLTY | 305–308 | NLTY | |
cA/GMP | 461–464 | KKIS | 461–464 | KKIS | 461–464 | KKIS | 461–464 | KKIS | 461–464 | KKIS | 461–464 | KKIS |
581–584 | RKRT | 581–584 | RKRT | 581–584 | RKRT | 581–584 | RKRT | 581–584 | RKRT | 581–584 | RKRT | |
PK C | 61–63 | SGK | 61–63 | SGK | 61–63 | SGK | 61–63 | SGK | 61–63 | SGK | 61–63 | SGK |
113–115 | SDK | 113–115 | SDK | 113–115 | SDK | 113–115 | SDK | 113–115 | SDK | 113–115 | SDK | |
265–267 | TAK | 265–267 | TAK | 265–267 | TAK | 265–267 | TAK | 265–267 | TAK | 265–267 | TAK | |
335–337 | TAK | 335–337 | TAK | 335–337 | TAK | 335–337 | TAK | 335–337 | TAK | |||
343–345 | SVK | 343–345 | SVK | 343–345 | SVK | 343–345 | SVK | 343–345 | SVK | 343–345 | SVK | |
350–352 | TAK | 350–352 | TAK | 350–352 | TAK | 350–352 | TAK | 350–352 | TAK | 350–352 | TAK | |
401–403 | SDK | 401–403 | SDK | |||||||||
615–617 | SKK | 615–617 | SKK | 615–617 | SKK | 615–617 | SKK | 615–617 | SKK | 615–617 | SKK | |
636–638 | TQK | 636–638 | TQK | 636–638 | TQK | |||||||
CK II | 75–78 | TAED | 75–78 | TAED | 75–78 | TAED | 75–78 | TAED | 75–78 | TAED | 75–78 | TAED |
123–126 | TYGE | 123–126 | TYGE | 123–126 | TYGE | 123–126 | TYGE | 123–126 | TYGE | 123–126 | TYGE | |
141–144 | TADD | 141–144 | TADD | 141–144 | TADD | 141–144 | TADD | 141–144 | TADD | 141–144 | TADD | |
204–207 | TMEE | 204–207 | TMEE | 204–207 | TMEE | 204–207 | TMEE | 204–207 | TMEE | 204–207 | TMEE | |
359–362 | TTFD | 359–362 | TTFD | 359–362 | TTFD | 359–362 | TTFD | 359–362 | TTFD | 359–362 | TTFD | |
401–404 | SDKD | 401–404 | SDKD | |||||||||
591–594 | SEWE | 591–594 | SEWE | 591–594 | SEWE | 591–594 | SEWE | 591–594 | SEWE | 591–594 | SEWE | |
425–428 | SKGE | 425–428 | SKGE | 425–428 | SKGE | 425–428 | SKGE | |||||
TK 1 | 582–589 | KRTEDFLY | 582–589 | KRTEDFLY | 582–589 | KRTEDFLY | 582–589 | KRTEDFLY | 582–589 | KRTEDFLY | 582–589 | KRTEDFLY |
N-myr | 26–31 | GTYWGV | 26–31 | GTYWGV | 26–31 | GTYWGV | 26–31 | GTYWGV | 26–31 | GTYWGV | 26–31 | GTYWGV |
45–50 | GVKAGR | 45–50 | GVKAGR | 45–50 | GVKAGR | 45–50 | GVKAGR | 45–50 | GVKAGR | 45–50 | GVKAGR | |
70–75 | GSQTGT | 70–75 | GSQTGT | 70–75 | GSQTGT | 70–75 | GSQTGT | 70–75 | GSQTGT | 70–75 | GSQTGT | |
150–155 | GNDPAL | 150–155 | GNDPAL | 150–155 | GNDPAL | 150–155 | GNDPAL | 150–155 | GNDPAL | 150–155 | GNDPAL | |
164–169 | GLGNNT | 164–169 | GLGNNT | 164–169 | GLGNNT | 164–169 | GLGNNT | 164–169 | GLGNNT | 164–169 | GLGNNT | |
303–308 | GSNLTY | 303–308 | GSNLTY | 303–308 | GSNLTY | 303–308 | GSNLTY | 303–308 | GSNLTY | 303–308 | GSNLTY | |
483–488 | GVATNY | 483–488 | GVATNY | 483–488 | GVATNY | 483–488 | GVATNY | 483–488 | GVATNY | 483–488 | GVATNY | |
568–573 | GLDVGR | 568–573 | GLDVGR | 568–573 | GLDVGR | 568–573 | GLDVGR | 568–573 | GLDVGR | 568–573 | GLDVGR | |
TK 2 | 398–405 | RLGSDKDY | 398–405 | RLGNDKDY | 398–405 | RLGNDKDY | 398–405 | RLGSDKDY | 398–405 | RLGNDKDY | 398–405 | RLGNDKDY |
582–589 | KRTEDFLY | 582–589 | KRTEDFLY | 582–589 | KRTEDFLY | 582–589 | KRTEDFLY | 582–589 | KRTEDFLY | 582–589 | KRTEDFLY |
Functional Site | H1 | H4 | H10/H12 | H29 | ||||
---|---|---|---|---|---|---|---|---|
Position | Sequence | Position | Sequence | Position | Sequence | Position | Sequence | |
N-gly | 255–258 | NITD | 255–258 | NITD | 255–258 | NITD | 255–258 | NITD |
429–432 | NFSL | 429–432 | NFSL | 429–432 | NFSL | 429–432 | NFSL | |
734–737 | NLSW | 734–737 | NLSW | 734–737 | NLSW | 734–737 | NLSW | |
cA/GMP | 190–193 | KRPS | 190–193 | KRPS | 190–193 | KRPS | 190–193 | KRPS |
225–228 | RKES | 225–228 | RKES | 225–228 | RKES | 225–228 | RKES | |
670–673 | RKVT | 670–673 | RKVT | 670–673 | RKVT | 670–673 | RKVT | |
809–812 | KRTS | 809–812 | KRTS | 809–812 | KRTS | 809–812 | KRTS | |
PK C | 112–114 | SIR | 112–114 | SIR | 112–114 | SIR | 112–114 | SIR |
230–232 | SGR | 230–232 | SGR | 230–232 | SGR | 230–232 | SGR | |
323–325 | TLR | 323–325 | TLR | 323–325 | TLR | 323–325 | TLR | |
446–448 | TIK | 446–448 | TIK | 446–448 | TIK | 446–448 | TIK | |
457–459 | SLR | 457–459 | SLR | 457–459 | SLR | 457–459 | SLR | |
673–675 | TLR | 673–675 | TLR | 673–675 | TLR | 673–675 | TLR | |
685–687 | SAR | 685–687 | SAR | 685–687 | SAR | 685–687 | SAR | |
694–696 | SVK | 694–696 | SVK | 694–696 | SVK | 694–696 | SVK | |
708–710 | TYR | 708–710 | TYR | 708–710 | TYR | 708–710 | TYR | |
740–742 | TLK | 740–742 | TLK | 740–742 | TLK | 740–742 | TLK | |
745–747 | SDR | 745–747 | SDR | 745–747 | SDR | 745–747 | SDR | |
774–776 | TKR | 774–776 | TKR | 774–776 | TKR | 774–776 | TKR | |
822–824 | SIK | 822–824 | SIK | 822–824 | SIK | 822–824 | SIK | |
189–191 | SKR | 189–191 | SKR | 189–191 | SKR | |||
CK II | 54–57 | STNE | 54–57 | STNE | 54–57 | STNE | 54–57 | STNE |
89–92 | TAYD | 89–92 | TAYD | 89–92 | TAYD | 89–92 | TAYD | |
246–249 | TTGD | 246–249 | TTGD | 246–249 | TTGD | 246–249 | TTGD | |
431–434 | SLDD | 431–434 | SLDD | 431–434 | SLDD | 431–434 | SLDD | |
463–466 | TPTE | 463–466 | TPTE | 463–466 | TPTE | 463–466 | TPTE | |
476–479 | TAAE | 476–479 | TAAE | 476–479 | TAAE | 476–479 | TAAE | |
568–471 | SWME | 568–471 | SWME | 568–471 | SWME | 568–471 | SWME | |
631–634 | SAFE | 631–634 | SAFE | 631–634 | SAFE | 631–634 | SAFE | |
657–660 | SGND | 657–660 | SGND | 657–660 | SGND | 657–660 | SGND | |
758–761 | SASD | 758–761 | SASD | 758–761 | SASD | 758–761 | SASD | |
812–815 | SILD | 812–815 | SILD | 812–815 | SILD | 812–815 | SILD | |
859–862 | SVLE | 859–862 | SVLE | 859–862 | SVLE | 859–862 | SVLE | |
881–884 | SGLE | 881–884 | SGLE | 881–884 | SGLE | |||
950–953 | SPDE | 950–953 | SPDE | 950–953 | SPDE | 950–953 | SPDE | |
N-myr | 82–87 | GVHDGL | 82–87 | GVHDGL | 82–87 | GVHDGL | 82–87 | GVHDGL |
242–247 | GVDPTT | 242–247 | GVDPTT | 242–247 | GVDPTT | 242–247 | GVDPTT | |
461–466 | GMTPTE | 461–466 | GMTPTE | |||||
491–496 | GIAHTR | 491–496 | GIAHTR | 491–496 | GIAHTR | 491–496 | GIAHTR | |
505–510 | GSNSGT | 505–510 | GSNSGT | 505–510 | GSNSGT | 505–510 | GSNSGT | |
557–562 | GQPPSN | 557–562 | GQPPSN | 557–562 | GQPPSN | 557–562 | GQPPSN | |
580–585 | GVSYAV | 580–585 | GVSYAV | 580–585 | GVSYAV | 580–585 | GVSYAV | |
650–655 | GTEDSS | 650–655 | GTEDSS | 650–655 | GTEDSS | 650–655 | GTEDSS | |
693–698 | GSVKNH | 693–698 | GSVKNH | 693–698 | GSVKNH | 693–698 | GSVKNH | |
828–833 | GSYLGM | 828–833 | GSYLGM | 828–833 | GSYLGM | 828–833 | GSYLGM | |
874–879 | GVATSF | 874–879 | GVATSF | 874–879 | GVATSF | 874–879 | GVATSF | |
882–887 | GLEPGE | 882–887 | GLEPGE | 882–887 | GLVPGE | 882–887 | GLEPGE | |
1035–1040 | GCKQGW | 1035–1040 | GCKQGW | 1035–1040 | GCKQGW | 1035–1040 | GCKQGW | |
Ami | 185–188 | CGKR | 185–188 | CGKR | 185–188 | CGKR | 185–188 | CGKR |
230–233 | SGRK | 230–233 | SGRK | 230–233 | SGRK | 230–233 | SGRK | |
402–405 | NGKR | 402–405 | NGKR | 402–405 | NGKR | 402–405 | NGKR | |
935–938 | AGRK | 935–938 | AGRK | 935–938 | AGRK | 935–938 | AGRK | |
CYP450 | 400–409 | FGNGKRACIG | 400–409 | FGNGKRACIG | 400–409 | FGNGKRACIG |
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Xu, Z.; Zhu, Y.; Xuan, L.; Li, S.; Cheng, Z. Haplotype Diversity of NADPH-Cytochrome P450 Reductase Gene of Ophiocordyceps sinensis and the Effect on Fungal Infection in Host Insects. Microorganisms 2020, 8, 968. https://doi.org/10.3390/microorganisms8070968
Xu Z, Zhu Y, Xuan L, Li S, Cheng Z. Haplotype Diversity of NADPH-Cytochrome P450 Reductase Gene of Ophiocordyceps sinensis and the Effect on Fungal Infection in Host Insects. Microorganisms. 2020; 8(7):968. https://doi.org/10.3390/microorganisms8070968
Chicago/Turabian StyleXu, Zixian, Yunguo Zhu, Lingyan Xuan, Shan Li, and Zhou Cheng. 2020. "Haplotype Diversity of NADPH-Cytochrome P450 Reductase Gene of Ophiocordyceps sinensis and the Effect on Fungal Infection in Host Insects" Microorganisms 8, no. 7: 968. https://doi.org/10.3390/microorganisms8070968